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The map label for this gene is mlhB [H]

Identifier: 117919062

GI number: 117919062

Start: 713656

End: 714567

Strand: Direct

Name: mlhB [H]

Synonym: Shewana3_0609

Alternate gene names: 117919062

Gene position: 713656-714567 (Clockwise)

Preceding gene: 117919061

Following gene: 117919063

Centisome position: 14.35

GC content: 51.21

Gene sequence:

>912_bases
ATGCCAAGCTGGCAAGCATCTGTATTAAATACTATTTTATCTTATGTGGCACGGCCATCCATCAGCCGTGGCAAGGCTAA
ACTCCCCCTTAATCAAGTACGGCAACGCTTATTAGAGCTGGATAAGCGCTGGCTGCCGTGGCCCGAAAAACTCGTTACGG
CCTCGATTCCCTTGCAACATTCTACCCTGTTGCATTATGTGCTGCTGAATGACACACCGCGTTTGGGCAATCTGTTTTAC
ATTCGAGGCGGCGGATTTTGTTTTAAAACTCCCCATGCCCACGCAAGACTGGTGGCCGATATCAGTGAGCGTTGTCGCCT
CGATACGTTTATCCCAGATTATCGTTTAGCGCCGGAGCATCCGTATCCCGCGCCCTGTGACGATGTGCTCGAGGCTTACC
TGCACTTAATTGAGTTGAAAGGCAGCGATAATCTGGTGTTGATGGGCGATTCCGCCGGCGGCAATTTAGCCTTGAGTCTG
CTGCTCGAACTGAAAAAGCGCCATCTGCCCATGCCTAAAGCCTGTGTGTTGTTGTCACCCGCGCTTGATTTAGCCATTAC
CGGCGATACCGAGCTGATTTTAGGGGCGGACGATCCGTTCTTTACCATTGAGTCGCTTTTGCGGCTGCGCGGCGCTTATC
TTGCTGGGGCGGATCCTATGTCTGAACGGGTTTCGCCTTTGCAGGGGAACTTGGCTGACTTGCCGCCACTCTTAGTGATT
GCCGGTACGCGCGAGTTGCTTTTACAGGACTCAGAACGACTGGTTATGCAAATTAAGGCCTGTGGTGGTGTGGTAGAATC
GCGTTTTTATCACAACATGCCCCATGTTTTTCCCCTGTTCCATCTGTTGCCAGAAGCGATTGAGGCGAGGGAACAAATTG
CAGATTTTGTGCTTTCACAGCTGATTCGTTAA

Upstream 100 bases:

>100_bases
CTGCGCCTAAAAGCACAGAATTATCGGAAGTTGGATCTGCTGTGATCTGCACGCCGTTCCCATCGGTGTCCTATTGCGGC
ACTGATGTGGAGGACGGGAA

Downstream 100 bases:

>100_bases
AATTGTGTAGTTATTCATATTCAGTGAATAGCTAGCAAAAATAGGGGTAAATAACTTTTTTTCTGACTAAATATGCTAAA
GGCCTCTGGTGTAAACGCTT

Product: alpha/beta hydrolase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MPSWQASVLNTILSYVARPSISRGKAKLPLNQVRQRLLELDKRWLPWPEKLVTASIPLQHSTLLHYVLLNDTPRLGNLFY
IRGGGFCFKTPHAHARLVADISERCRLDTFIPDYRLAPEHPYPAPCDDVLEAYLHLIELKGSDNLVLMGDSAGGNLALSL
LLELKKRHLPMPKACVLLSPALDLAITGDTELILGADDPFFTIESLLRLRGAYLAGADPMSERVSPLQGNLADLPPLLVI
AGTRELLLQDSERLVMQIKACGGVVESRFYHNMPHVFPLFHLLPEAIEAREQIADFVLSQLIR

Sequences:

>Translated_303_residues
MPSWQASVLNTILSYVARPSISRGKAKLPLNQVRQRLLELDKRWLPWPEKLVTASIPLQHSTLLHYVLLNDTPRLGNLFY
IRGGGFCFKTPHAHARLVADISERCRLDTFIPDYRLAPEHPYPAPCDDVLEAYLHLIELKGSDNLVLMGDSAGGNLALSL
LLELKKRHLPMPKACVLLSPALDLAITGDTELILGADDPFFTIESLLRLRGAYLAGADPMSERVSPLQGNLADLPPLLVI
AGTRELLLQDSERLVMQIKACGGVVESRFYHNMPHVFPLFHLLPEAIEAREQIADFVLSQLIR
>Mature_302_residues
PSWQASVLNTILSYVARPSISRGKAKLPLNQVRQRLLELDKRWLPWPEKLVTASIPLQHSTLLHYVLLNDTPRLGNLFYI
RGGGFCFKTPHAHARLVADISERCRLDTFIPDYRLAPEHPYPAPCDDVLEAYLHLIELKGSDNLVLMGDSAGGNLALSLL
LELKKRHLPMPKACVLLSPALDLAITGDTELILGADDPFFTIESLLRLRGAYLAGADPMSERVSPLQGNLADLPPLLVIA
GTRELLLQDSERLVMQIKACGGVVESRFYHNMPHVFPLFHLLPEAIEAREQIADFVLSQLIR

Specific function: Lactone hydrolase that is required for the degradation of monocyclic monoterpenes such as menthol and carvone. Catalyzes the ring opening of lactones formed during degradation of monocyclic monoterpenes. Can hydrolyze epsilon-caprolactone, the lactone der

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1786682, Length=240, Percent_Identity=28.3333333333333, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.83 [H]

Molecular weight: Translated: 33799; Mature: 33668

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSWQASVLNTILSYVARPSISRGKAKLPLNQVRQRLLELDKRWLPWPEKLVTASIPLQH
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHEEECCCCCH
STLLHYVLLNDTPRLGNLFYIRGGGFCFKTPHAHARLVADISERCRLDTFIPDYRLAPEH
HHHEEEEEECCCCCCCCEEEEECCCEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCC
PYPAPCDDVLEAYLHLIELKGSDNLVLMGDSAGGNLALSLLLELKKRHLPMPKACVLLSP
CCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHEEECC
ALDLAITGDTELILGADDPFFTIESLLRLRGAYLAGADPMSERVSPLQGNLADLPPLLVI
CCCEEEECCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCEEEE
AGTRELLLQDSERLVMQIKACGGVVESRFYHNMPHVFPLFHLLPEAIEAREQIADFVLSQ
ECCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LIR
HCC
>Mature Secondary Structure 
PSWQASVLNTILSYVARPSISRGKAKLPLNQVRQRLLELDKRWLPWPEKLVTASIPLQH
CCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHEEECCCCCH
STLLHYVLLNDTPRLGNLFYIRGGGFCFKTPHAHARLVADISERCRLDTFIPDYRLAPEH
HHHEEEEEECCCCCCCCEEEEECCCEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCC
PYPAPCDDVLEAYLHLIELKGSDNLVLMGDSAGGNLALSLLLELKKRHLPMPKACVLLSP
CCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHEEECC
ALDLAITGDTELILGADDPFFTIESLLRLRGAYLAGADPMSERVSPLQGNLADLPPLLVI
CCCEEEECCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCEEEE
AGTRELLLQDSERLVMQIKACGGVVESRFYHNMPHVFPLFHLLPEAIEAREQIADFVLSQ
ECCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LIR
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA