Click here to switch to the map view.

The map label for this gene is ampD [H]

Identifier: 117918880

GI number: 117918880

Start: 482516

End: 483061

Strand: Direct

Name: ampD [H]

Synonym: Shewana3_0423

Alternate gene names: 117918880

Gene position: 482516-483061 (Clockwise)

Preceding gene: 117918877

Following gene: 117918881

Centisome position: 9.7

GC content: 48.35

Gene sequence:

>546_bases
ATGAGTTTTGAGTTTGGTTGGTATCGCGCGGCGCGGCGCTGTGAATCACCACATTTTAACCGTCGGCCCTTTGGAGAGAT
CAGCCTGTTGGTGATCCACAATATTAGTTTGCCCGCGGGATGCTTTGGGCTGCCTTATATTGATCAATTATTTCAAGGGT
GTTTAGATACCAGTGCCGATGAAAGTTTCGCCGATTTAGCTGGGCTGGAGGTGTCGGCTCATTTTTTAATTCGCCGCGAT
GGCGAATGTGTGCAGTATGTGAGTTGTGAAGATAGAGCTTGGCACGCGGGTGTATCCCGTTATGGCGAAAGGGAAAATTG
TAATGATTTCTCTATTGGTATTGAACTTGAAGGTACGGATACTGAACCCTATACTGCCGCCCAATATCAGCAACTTACCG
CATTGACCCAAGCTTTATTGGCACAATATCCTGCGTTAACCGCAGAGCGGATTGTGGGACATTGCGATATTGCACCAATG
CGGAAGACTGACCCAGGCCCAAGCTTTGACTGGCAAAGATATTTAGCGGCGCTGCGCGACTTTTAG

Upstream 100 bases:

>100_bases
CTTCGTGGCTCTAGAATCTAGCACCTAGAACCTAGCACCTAGAACCCAGAACCTAGAACCTAGCCGTCTCGTAGTGACGT
TCCGTTTTTAAGGAGTAAAC

Downstream 100 bases:

>100_bases
AATATAGGAGGTTGCTGCGCAACTGCTAGGACGCAGCAAAAGCGCTGCTCTAGGACGCTACTTCGTAGCTCTAGAGCCTA
GAACCTAGAACCTAGAACCT

Product: N-acetyl-anhydromuranmyl-L-alanine amidase

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase [H]

Number of amino acids: Translated: 181; Mature: 180

Protein sequence:

>181_residues
MSFEFGWYRAARRCESPHFNRRPFGEISLLVIHNISLPAGCFGLPYIDQLFQGCLDTSADESFADLAGLEVSAHFLIRRD
GECVQYVSCEDRAWHAGVSRYGERENCNDFSIGIELEGTDTEPYTAAQYQQLTALTQALLAQYPALTAERIVGHCDIAPM
RKTDPGPSFDWQRYLAALRDF

Sequences:

>Translated_181_residues
MSFEFGWYRAARRCESPHFNRRPFGEISLLVIHNISLPAGCFGLPYIDQLFQGCLDTSADESFADLAGLEVSAHFLIRRD
GECVQYVSCEDRAWHAGVSRYGERENCNDFSIGIELEGTDTEPYTAAQYQQLTALTQALLAQYPALTAERIVGHCDIAPM
RKTDPGPSFDWQRYLAALRDF
>Mature_180_residues
SFEFGWYRAARRCESPHFNRRPFGEISLLVIHNISLPAGCFGLPYIDQLFQGCLDTSADESFADLAGLEVSAHFLIRRDG
ECVQYVSCEDRAWHAGVSRYGERENCNDFSIGIELEGTDTEPYTAAQYQQLTALTQALLAQYPALTAERIVGHCDIAPMR
KTDPGPSFDWQRYLAALRDF

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1786300, Length=175, Percent_Identity=63.4285714285714, Blast_Score=217, Evalue=4e-58,
Organism=Escherichia coli, GI1787092, Length=130, Percent_Identity=39.2307692307692, Blast_Score=85, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 20425; Mature: 20294

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFEFGWYRAARRCESPHFNRRPFGEISLLVIHNISLPAGCFGLPYIDQLFQGCLDTSAD
CCCCCHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCC
ESFADLAGLEVSAHFLIRRDGECVQYVSCEDRAWHAGVSRYGERENCNDFSIGIELEGTD
CHHHHHHCCCEEEEEEEECCCCEEEEECCCCCHHHHHHHHCCCCCCCCCEEEEEEEECCC
TEPYTAAQYQQLTALTQALLAQYPALTAERIVGHCDIAPMRKTDPGPSFDWQRYLAALRD
CCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHC
F
C
>Mature Secondary Structure 
SFEFGWYRAARRCESPHFNRRPFGEISLLVIHNISLPAGCFGLPYIDQLFQGCLDTSAD
CCCCHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCC
ESFADLAGLEVSAHFLIRRDGECVQYVSCEDRAWHAGVSRYGERENCNDFSIGIELEGTD
CHHHHHHCCCEEEEEEEECCCCEEEEECCCCCHHHHHHHHCCCCCCCCCEEEEEEEECCC
TEPYTAAQYQQLTALTQALLAQYPALTAERIVGHCDIAPMRKTDPGPSFDWQRYLAALRD
CCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHC
F
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8383940 [H]