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The map label for this gene is ampE [H]

Identifier: 117918881

GI number: 117918881

Start: 483246

End: 484097

Strand: Direct

Name: ampE [H]

Synonym: Shewana3_0424

Alternate gene names: 117918881

Gene position: 483246-484097 (Clockwise)

Preceding gene: 117918880

Following gene: 117918882

Centisome position: 9.72

GC content: 47.65

Gene sequence:

>852_bases
ATGGCATTATTTTCATTACTGGTCGCCATTCTCGTAGAACGATTGAAGTTTCTTCCTGCCTCTTGGCAATGCGATCGCGT
GCTGCAATCCTATCAATCGACCTTTTTTGGTGATAAAGCTTCTCTTACTAGTACTAACATGTTGCTCGCCCTCGTGTTAC
CGGCTTTGGTCGTTCATGTTTTGGGGTGGGTTGCGTCAGGCATGTTCTGGGGGCTGTTAACTCTTGCCCTGTGGGTGGTG
GTGGCAATTATTTGCTTTAATCATCAAAAACAGCGCGATAGCTTCAAAAAGTATATGCAGGCGGCGTGTCGCTCCGATGT
GCAAGCCTGTTACCACTATGCCGCCGAACTAGATTGCAGTGAATGTTTGGATGCGGTGTCTGAAAAAGACTTAGGCGCTA
AGGTCGGGCAGAGTGTTGCCTGGATTAACTATCGTTATTACGGCGCAGTGGCATTATGTTTTATCTTCCTAGGCCCAGTC
GGCGCGGTGTTATATTGCACCGCTCGCTTTTATGCCGAAGAGAATGTGCGTAAGTCGCTTAATTTACCGCTGATTGAAGA
TATTATGTTTGTTATGGATTGGATCCCGAGTCGGATATTTTCCTTTGGCTATGCGCTCAGCGGTCAGTTTAGCGAAGGGC
TCTCGGCTTGGCGTGACCATGCCTTAAATATCCATGCGAGTGCCAGACGAGTAGTCACTGAAACGGCATTAGCGGCTCAA
CCGCTGCCGGAGGAGTCTAGTGCACCAGTTTGTGTACAATCGACCTTAGCATTATTAGTCTTAAGTAAACGCAACTTTAC
GCTGATGGTTGCTGTGCTATCGCTGTTGACCATTTTTGGGTTGGTGAGTTAA

Upstream 100 bases:

>100_bases
CTAGAACCTAGAACCTAGAACCTAGAACCTAGGGCCTAGAAGGGCGAAGCCCGCGCTGCTTTTTCTAGTCCGTCCAGCTT
TTTTACAAGGAGTATTACTG

Downstream 100 bases:

>100_bases
TAAGAAGGACACTGCGCGGCTTCTAGAATCTAGGACGCTTTGCTGCTAGGACGCAGCAAAAAGCGCTGCTCTAGGACGCC
ACTTCGTGGCTCTAGAATCT

Product: regulatory protein AmpE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MALFSLLVAILVERLKFLPASWQCDRVLQSYQSTFFGDKASLTSTNMLLALVLPALVVHVLGWVASGMFWGLLTLALWVV
VAIICFNHQKQRDSFKKYMQAACRSDVQACYHYAAELDCSECLDAVSEKDLGAKVGQSVAWINYRYYGAVALCFIFLGPV
GAVLYCTARFYAEENVRKSLNLPLIEDIMFVMDWIPSRIFSFGYALSGQFSEGLSAWRDHALNIHASARRVVTETALAAQ
PLPEESSAPVCVQSTLALLVLSKRNFTLMVAVLSLLTIFGLVS

Sequences:

>Translated_283_residues
MALFSLLVAILVERLKFLPASWQCDRVLQSYQSTFFGDKASLTSTNMLLALVLPALVVHVLGWVASGMFWGLLTLALWVV
VAIICFNHQKQRDSFKKYMQAACRSDVQACYHYAAELDCSECLDAVSEKDLGAKVGQSVAWINYRYYGAVALCFIFLGPV
GAVLYCTARFYAEENVRKSLNLPLIEDIMFVMDWIPSRIFSFGYALSGQFSEGLSAWRDHALNIHASARRVVTETALAAQ
PLPEESSAPVCVQSTLALLVLSKRNFTLMVAVLSLLTIFGLVS
>Mature_282_residues
ALFSLLVAILVERLKFLPASWQCDRVLQSYQSTFFGDKASLTSTNMLLALVLPALVVHVLGWVASGMFWGLLTLALWVVV
AIICFNHQKQRDSFKKYMQAACRSDVQACYHYAAELDCSECLDAVSEKDLGAKVGQSVAWINYRYYGAVALCFIFLGPVG
AVLYCTARFYAEENVRKSLNLPLIEDIMFVMDWIPSRIFSFGYALSGQFSEGLSAWRDHALNIHASARRVVTETALAAQP
LPEESSAPVCVQSTLALLVLSKRNFTLMVAVLSLLTIFGLVS

Specific function: Putative signaling protein in beta-lactamase regulation. AmpE seems not to act as a direct sensor for beta-lactams [H]

COG id: COG3725

COG function: function code V; Membrane protein required for beta-lactamase induction

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786301, Length=295, Percent_Identity=27.1186440677966, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: NA

Molecular weight: Translated: 31360; Mature: 31229

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ALFSLLVAILVERLKFLPASWQCDRVLQSYQSTFFGDKASLTSTNMLLALVLPALVVHV
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
LGWVASGMFWGLLTLALWVVVAIICFNHQKQRDSFKKYMQAACRSDVQACYHYAAELDCS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
ECLDAVSEKDLGAKVGQSVAWINYRYYGAVALCFIFLGPVGAVLYCTARFYAEENVRKSL
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NLPLIEDIMFVMDWIPSRIFSFGYALSGQFSEGLSAWRDHALNIHASARRVVTETALAAQ
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
PLPEESSAPVCVQSTLALLVLSKRNFTLMVAVLSLLTIFGLVS
CCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2691840; 2607970; 9278503 [H]