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The map label for this gene is tesA [H]

Identifier: 116689940

GI number: 116689940

Start: 2130940

End: 2131608

Strand: Direct

Name: tesA [H]

Synonym: Bcen2424_1919

Alternate gene names: 116689940

Gene position: 2130940-2131608 (Clockwise)

Preceding gene: 116689938

Following gene: 116689946

Centisome position: 61.17

GC content: 69.36

Gene sequence:

>669_bases
ATGGACACGACATTTCGCTGGAAAAGACGCGCGGCGGCCGCCGCGCTGCTGGGCACCCTGCTGGCCGTCACCGTGCCCGC
ACGCGCCGCGACGGCGCCGGCATCGGGCCCGCCCGTGATCGTCGTCCTCGGCGACAGTCTCTCGGCCGAATACGGCTTGC
CGCGCGACACCGGCTGGGTGGCGTTACTGCGGCAACGGCTCACGACCGAGCGAATCGATTATAGCGTCGCGAACGCCAGC
GTCAGCGGCGACACCACGAGCGGCGGCCGTGCGCGGCTGCCCGCGGTGCTGCAGCGGCTCAAGCCGTCGATCGTCGTCGT
CGAACTCGGCTCGAACGACGCGCTGCGCGGCGTACCGCTCGCGACGACCGAGCAGAACCTGCGCGACATCATCGCCAGCG
CGCAGCGCGCGCGGGCGAAGGTCGTGCTGGTCGGCATGTACGTGCCACCCAATTACGGCCCCGACTACACGCAGAAGTTC
CACGCGGTCTATACGCGGCTGTCGAAAGACCTCGGCGTGCCGCTCGTGCCATTCCTGCTGGCCGGCATCGAGAACAAGCC
CGAGCTGTTCCAGGCCGACCAGATGCATCCCGCGCAGCAGGCGCAGGGCATCCTGCTCGACAATGTGTGGCCGGCACTGA
AGCCGTTGCTCGGCAAGCCGCGCGGCTGA

Upstream 100 bases:

>100_bases
GGCGAGCGTGATGCCGTCGAGGATCGTCAGCTCGCCCGTTGCATCGGCAACCCGCTTGCACACGTCATGGACTTCGATGA
TCGGATCGGTAATCTTGAAC

Downstream 100 bases:

>100_bases
CCGCAGCCGAGCGTCGCAAAACGAAAAGCCCCGCTTCGAGCGGGGCTTTTTGATGGTTGCGGGCGAAACGAGGCTTAGTT
GTCGCCGTCCTGCTGCTGGC

Product: GDSL family lipase

Products: NA

Alternate protein names: Lecithinase B; Lysophospholipase L1; Protease I [H]

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MDTTFRWKRRAAAAALLGTLLAVTVPARAATAPASGPPVIVVLGDSLSAEYGLPRDTGWVALLRQRLTTERIDYSVANAS
VSGDTTSGGRARLPAVLQRLKPSIVVVELGSNDALRGVPLATTEQNLRDIIASAQRARAKVVLVGMYVPPNYGPDYTQKF
HAVYTRLSKDLGVPLVPFLLAGIENKPELFQADQMHPAQQAQGILLDNVWPALKPLLGKPRG

Sequences:

>Translated_222_residues
MDTTFRWKRRAAAAALLGTLLAVTVPARAATAPASGPPVIVVLGDSLSAEYGLPRDTGWVALLRQRLTTERIDYSVANAS
VSGDTTSGGRARLPAVLQRLKPSIVVVELGSNDALRGVPLATTEQNLRDIIASAQRARAKVVLVGMYVPPNYGPDYTQKF
HAVYTRLSKDLGVPLVPFLLAGIENKPELFQADQMHPAQQAQGILLDNVWPALKPLLGKPRG
>Mature_222_residues
MDTTFRWKRRAAAAALLGTLLAVTVPARAATAPASGPPVIVVLGDSLSAEYGLPRDTGWVALLRQRLTTERIDYSVANAS
VSGDTTSGGRARLPAVLQRLKPSIVVVELGSNDALRGVPLATTEQNLRDIIASAQRARAKVVLVGMYVPPNYGPDYTQKF
HAVYTRLSKDLGVPLVPFLLAGIENKPELFQADQMHPAQQAQGILLDNVWPALKPLLGKPRG

Specific function: Hydrolyzes only long chain acyl thioesters (C12-C18). Specificity similar to chymotrypsin [H]

COG id: COG2755

COG function: function code E; Lysophospholipase L1 and related esterases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDSL' lipolytic enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1786702, Length=219, Percent_Identity=38.8127853881278, Blast_Score=147, Evalue=5e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013830
- InterPro:   IPR013831
- InterPro:   IPR001087
- InterPro:   IPR008265 [H]

Pfam domain/function: PF00657 Lipase_GDSL [H]

EC number: =3.1.1.5 [H]

Molecular weight: Translated: 23808; Mature: 23808

Theoretical pI: Translated: 10.48; Mature: 10.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTTFRWKRRAAAAALLGTLLAVTVPARAATAPASGPPVIVVLGDSLSAEYGLPRDTGWV
CCCCHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCEEEEECCCCCCCCCCCCCHHHH
ALLRQRLTTERIDYSVANASVSGDTTSGGRARLPAVLQRLKPSIVVVELGSNDALRGVPL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCC
ATTEQNLRDIIASAQRARAKVVLVGMYVPPNYGPDYTQKFHAVYTRLSKDLGVPLVPFLL
CCCHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHH
AGIENKPELFQADQMHPAQQAQGILLDNVWPALKPLLGKPRG
HCCCCCCHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDTTFRWKRRAAAAALLGTLLAVTVPARAATAPASGPPVIVVLGDSLSAEYGLPRDTGWV
CCCCHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCEEEEECCCCCCCCCCCCCHHHH
ALLRQRLTTERIDYSVANASVSGDTTSGGRARLPAVLQRLKPSIVVVELGSNDALRGVPL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCC
ATTEQNLRDIIASAQRARAKVVLVGMYVPPNYGPDYTQKFHAVYTRLSKDLGVPLVPFLL
CCCHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHH
AGIENKPELFQADQMHPAQQAQGILLDNVWPALKPLLGKPRG
HCCCCCCHHHCCCCCCHHHHHCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]