Click here to switch to the map view.

The map label for this gene is rbsB [H]

Identifier: 116689563

GI number: 116689563

Start: 1703915

End: 1704859

Strand: Reverse

Name: rbsB [H]

Synonym: Bcen2424_1541

Alternate gene names: 116689563

Gene position: 1704859-1703915 (Counterclockwise)

Preceding gene: 116689564

Following gene: 116689562

Centisome position: 48.94

GC content: 65.5

Gene sequence:

>945_bases
ATGTTCAAGCACACCGCCGCCCTGACCGCCGTCGCCTGCGCACTCGCCTTCGGCGCTGCCGCCGCCCAGGCAGCCGACAA
GCCGCTGAAATCGATCGGCGTCACGGTCGGCTCGCTCGGCAACCCGTACTTCGTCACGATCGTGAAAGGCGCCGAAGCGC
GCGCGAAGCAGCTCAACCCGAACGCGAAAGTCACGGCCGTCTCGGCCGACTACGACCTGAACAAGCAGTTCACGCAGATC
GACAACTTCATTTCCGCGCACGTCGACATGATCCTGCTCAACGCGACCGACCCGAAGGCGATCGAGCCGGCGGTGAAAAA
GGCGCAGGCGGCCGGCATCACGGTCGTCGCGGTGGACGTCGCGGCGGCCGGCGCGAACGCGACCGTGCAGACCAACAACG
TGAAGGCCGGCGAACTCGCGTGCGACTACCTCGCGAAGAAGCTGAACGGCAAGGGCAACGTGATCATCGAGAACGGCCCG
CAGGTGTCGGCCGTGATCGATCGCGTGAACGGCTGCAAGGCCGTGCTCGCGAAAAACGCCGGCATCAAGCTGCTGTCGAG
CGACCAGGACGGCAAGGGCTCCCGCGAAGGCGGGATGAACGCGATGCAGGGCTACCTGACGCGCTTTCCGAAGCTCGACG
GCGTGTTCACGATCAACGACCCGCAGGCGATCGGCAGCGATCTCGCGGCGAAACAGCTGAACCGCCCGAACATCGTGATC
ACGTCGGTCGACGGCGCGCCCGACATCGAGGTCGCACTCAAGTCGAACACGCTCGTGCAAGCGTCGTCGAGCCAGGACCC
GTGGGCGATGGCCCAGCAGGCCGTCAACGTCGGCTACGGGATCATGAACGGCCAGAAGCCGGCCAATCCGATGATCCTGA
TCGAGCCGACGCTGATCACGCGCGACAACGTGAAGACCTACAAGGGCTGGAGCTCCCCGCGCTGA

Upstream 100 bases:

>100_bases
CGGCCCGTACCTGACTCCCTTCCCTCATCGTTCAACCATCGGCGCGCAGGCAGGTCCTCCTGCGCGCGTTCACCAACAGA
CCTCCCGGAGACACAACGAC

Downstream 100 bases:

>100_bases
TCACGCCGATCGCCCGCGAGGCCGGGCAGTGCCGGGCCGCGCCGCCACGCGCGACGCCCCGCACCGCCCGGCTGTTCAGA
TTCAGATCAACCGCTGTACG

Product: periplasmic binding protein/LacI transcriptional regulator

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MFKHTAALTAVACALAFGAAAAQAADKPLKSIGVTVGSLGNPYFVTIVKGAEARAKQLNPNAKVTAVSADYDLNKQFTQI
DNFISAHVDMILLNATDPKAIEPAVKKAQAAGITVVAVDVAAAGANATVQTNNVKAGELACDYLAKKLNGKGNVIIENGP
QVSAVIDRVNGCKAVLAKNAGIKLLSSDQDGKGSREGGMNAMQGYLTRFPKLDGVFTINDPQAIGSDLAAKQLNRPNIVI
TSVDGAPDIEVALKSNTLVQASSSQDPWAMAQQAVNVGYGIMNGQKPANPMILIEPTLITRDNVKTYKGWSSPR

Sequences:

>Translated_314_residues
MFKHTAALTAVACALAFGAAAAQAADKPLKSIGVTVGSLGNPYFVTIVKGAEARAKQLNPNAKVTAVSADYDLNKQFTQI
DNFISAHVDMILLNATDPKAIEPAVKKAQAAGITVVAVDVAAAGANATVQTNNVKAGELACDYLAKKLNGKGNVIIENGP
QVSAVIDRVNGCKAVLAKNAGIKLLSSDQDGKGSREGGMNAMQGYLTRFPKLDGVFTINDPQAIGSDLAAKQLNRPNIVI
TSVDGAPDIEVALKSNTLVQASSSQDPWAMAQQAVNVGYGIMNGQKPANPMILIEPTLITRDNVKTYKGWSSPR
>Mature_314_residues
MFKHTAALTAVACALAFGAAAAQAADKPLKSIGVTVGSLGNPYFVTIVKGAEARAKQLNPNAKVTAVSADYDLNKQFTQI
DNFISAHVDMILLNATDPKAIEPAVKKAQAAGITVVAVDVAAAGANATVQTNNVKAGELACDYLAKKLNGKGNVIIENGP
QVSAVIDRVNGCKAVLAKNAGIKLLSSDQDGKGSREGGMNAMQGYLTRFPKLDGVFTINDPQAIGSDLAAKQLNRPNIVI
TSVDGAPDIEVALKSNTLVQASSSQDPWAMAQQAVNVGYGIMNGQKPANPMILIEPTLITRDNVKTYKGWSSPR

Specific function: Involved in the high-affinity D-ribose membrane transport system [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=289, Percent_Identity=29.0657439446367, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 32728; Mature: 32728

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKHTAALTAVACALAFGAAAAQAADKPLKSIGVTVGSLGNPYFVTIVKGAEARAKQLNP
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCEEECCCCCCEEEEEEECCCHHHHHCCC
NAKVTAVSADYDLNKQFTQIDNFISAHVDMILLNATDPKAIEPAVKKAQAAGITVVAVDV
CCEEEEEECCCCCCHHHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHHHHCCEEEEEEEE
AAAGANATVQTNNVKAGELACDYLAKKLNGKGNVIIENGPQVSAVIDRVNGCKAVLAKNA
EECCCCCEEEECCCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHCCCCEEEECCC
GIKLLSSDQDGKGSREGGMNAMQGYLTRFPKLDGVFTINDPQAIGSDLAAKQLNRPNIVI
CEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHCCCCCEEE
TSVDGAPDIEVALKSNTLVQASSSQDPWAMAQQAVNVGYGIMNGQKPANPMILIEPTLIT
EECCCCCCEEEEEECCEEEEECCCCCHHHHHHHHHHCCEEECCCCCCCCCEEEEECCEEE
RDNVKTYKGWSSPR
CCCCCEECCCCCCC
>Mature Secondary Structure
MFKHTAALTAVACALAFGAAAAQAADKPLKSIGVTVGSLGNPYFVTIVKGAEARAKQLNP
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCEEECCCCCCEEEEEEECCCHHHHHCCC
NAKVTAVSADYDLNKQFTQIDNFISAHVDMILLNATDPKAIEPAVKKAQAAGITVVAVDV
CCEEEEEECCCCCCHHHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHHHHCCEEEEEEEE
AAAGANATVQTNNVKAGELACDYLAKKLNGKGNVIIENGPQVSAVIDRVNGCKAVLAKNA
EECCCCCEEEECCCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHCCCCEEEECCC
GIKLLSSDQDGKGSREGGMNAMQGYLTRFPKLDGVFTINDPQAIGSDLAAKQLNRPNIVI
CEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHCCCCCEEE
TSVDGAPDIEVALKSNTLVQASSSQDPWAMAQQAVNVGYGIMNGQKPANPMILIEPTLIT
EECCCCCCEEEEEECCEEEEECCCCCHHHHHHHHHHCCEEECCCCCCCCCEEEEECCEEE
RDNVKTYKGWSSPR
CCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 7921236 [H]