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The map label for this gene is rhmD [H]

Identifier: 116624401

GI number: 116624401

Start: 6671129

End: 6672301

Strand: Reverse

Name: rhmD [H]

Synonym: Acid_5323

Alternate gene names: 116624401

Gene position: 6672301-6671129 (Counterclockwise)

Preceding gene: 116624402

Following gene: 116624400

Centisome position: 66.95

GC content: 62.66

Gene sequence:

>1173_bases
ATGAAGATCACTGAGATTCGCACCCGCGTCGTCGAGTGGCGCGGCAAGACCGTGCCGCCGCAGCCGCACTTCTGCACCAA
CCCAATGGACATCCTCGACCTTCCGGCCGATTCCATGGGGAGCTTTCGCTTCCATGGCTGGTTGATCGTGGAGGTCTTCA
CCGACGCCGGCATCGTGGGCATCGGCAATGCCGCGCTCTCACCGCGCGTCACCAAGCAGCTGATCGATGTATACCTGAAG
CCGCTGCTGCTCGGCAAAGACCCCTTCGATTACGAGTTCCTCTGGCAGCACATGTACCGGCAGACTATGGCATTCGGCCG
CAAAGGTGTCGCCATGGTGGCGATCAGCGCGCTCGATATTGCCATCTGGGACATCCTCGGCAAAGCCGCGAAGCAGCCCG
TCTTCAAACTGCTCGGCGGCCGCACCAAGCAGAAGATCCCGGTCTACGCCAGCCGTCTCTATAGCCAGCCGCTCGACCAG
TTGGCCGCCGAGGCGAAGCTGTACAAGGACCAGGGATATCGCGCGATGAAACTTCGCTTCGGCTGGGGTCCGGTGGATGG
CGCGGCGGGGATGCAGCGCAATGTCGAACTGGTGCGCACCGTGCGCGAAGTGGTCGGCAGCGAGATCGATGTGATGGCCG
ATGCCTACATGGGCTGGACCCTCGACTACGCGCGCCGCATGCTGCCGCTGCTCGAGTCCTTCCACCTGCGCTGGCTCGAA
GAGCCCGTCATCCCCGACGATATTCACGGCTATGCCGCGCTCAAGGCGCTCGGCCGGATCCCGATCGCGGGAGGCGAGCA
CGAGCACACCTCGTACGGCTTCCGCGAACTGCTGGAAGCCAAGGCCGTTGACTACATTCAGTTCGACACCAACCGCGTCG
GCGGCATCACGCAGGCGCGCAAGGTGCAGTCCCTCGCCGAGGCCTTCTCGGTGCCGGTCGTGCCGCATGCCGGGCAGATG
CACAACTTCCACGTCGTGATGGCGAGCCTGAATTCGCCGATGGCGGAGTTCTTCCCGGTTGTGGATGTGGAGATCGGCAA
CGAGCTCTTCTGGTACATCTTCGAGGGCGAGCCAACGCCGATGGATGGCTATATCGATTTGCGTGACGACGTTCCCGGGC
TGGGGCTGACTATCAAGGAAGACGCGCTCGGCCAGTTTGCGATCATCGAATAA

Upstream 100 bases:

>100_bases
TGGTGAGCACCATGCACGTGATCGCATTCGGCGTCATCCTGCTGACCGTGCGCAAGGTCGCGCCGCTGGCGGCGTTCCGC
GAGAAGATAGAAAAACGACC

Downstream 100 bases:

>100_bases
TCGCTATGCGCCTGATTCAACTTCATGGCCCCGAAGGCCGCCGGGTCGGCATCGTGGATGGTGACTTCATCCGCCTTTTG
AACGAAGCCCGGTCTATCTA

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: RhamD [H]

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MKITEIRTRVVEWRGKTVPPQPHFCTNPMDILDLPADSMGSFRFHGWLIVEVFTDAGIVGIGNAALSPRVTKQLIDVYLK
PLLLGKDPFDYEFLWQHMYRQTMAFGRKGVAMVAISALDIAIWDILGKAAKQPVFKLLGGRTKQKIPVYASRLYSQPLDQ
LAAEAKLYKDQGYRAMKLRFGWGPVDGAAGMQRNVELVRTVREVVGSEIDVMADAYMGWTLDYARRMLPLLESFHLRWLE
EPVIPDDIHGYAALKALGRIPIAGGEHEHTSYGFRELLEAKAVDYIQFDTNRVGGITQARKVQSLAEAFSVPVVPHAGQM
HNFHVVMASLNSPMAEFFPVVDVEIGNELFWYIFEGEPTPMDGYIDLRDDVPGLGLTIKEDALGQFAIIE

Sequences:

>Translated_390_residues
MKITEIRTRVVEWRGKTVPPQPHFCTNPMDILDLPADSMGSFRFHGWLIVEVFTDAGIVGIGNAALSPRVTKQLIDVYLK
PLLLGKDPFDYEFLWQHMYRQTMAFGRKGVAMVAISALDIAIWDILGKAAKQPVFKLLGGRTKQKIPVYASRLYSQPLDQ
LAAEAKLYKDQGYRAMKLRFGWGPVDGAAGMQRNVELVRTVREVVGSEIDVMADAYMGWTLDYARRMLPLLESFHLRWLE
EPVIPDDIHGYAALKALGRIPIAGGEHEHTSYGFRELLEAKAVDYIQFDTNRVGGITQARKVQSLAEAFSVPVVPHAGQM
HNFHVVMASLNSPMAEFFPVVDVEIGNELFWYIFEGEPTPMDGYIDLRDDVPGLGLTIKEDALGQFAIIE
>Mature_390_residues
MKITEIRTRVVEWRGKTVPPQPHFCTNPMDILDLPADSMGSFRFHGWLIVEVFTDAGIVGIGNAALSPRVTKQLIDVYLK
PLLLGKDPFDYEFLWQHMYRQTMAFGRKGVAMVAISALDIAIWDILGKAAKQPVFKLLGGRTKQKIPVYASRLYSQPLDQ
LAAEAKLYKDQGYRAMKLRFGWGPVDGAAGMQRNVELVRTVREVVGSEIDVMADAYMGWTLDYARRMLPLLESFHLRWLE
EPVIPDDIHGYAALKALGRIPIAGGEHEHTSYGFRELLEAKAVDYIQFDTNRVGGITQARKVQSLAEAFSVPVVPHAGQM
HNFHVVMASLNSPMAEFFPVVDVEIGNELFWYIFEGEPTPMDGYIDLRDDVPGLGLTIKEDALGQFAIIE

Specific function: Catalyzes the dehydration of L-rhamnonate to 2-keto-3- deoxy-L-rhamnonate (KDR) [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. RhamD subfamily [H]

Homologues:

Organism=Homo sapiens, GI42544119, Length=362, Percent_Identity=27.9005524861878, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI186972148, Length=262, Percent_Identity=30.9160305343511, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI42544117, Length=328, Percent_Identity=26.5243902439024, Blast_Score=99, Evalue=5e-21,
Organism=Escherichia coli, GI226510960, Length=342, Percent_Identity=29.5321637426901, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI48994953, Length=350, Percent_Identity=30, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI1787864, Length=374, Percent_Identity=25.4010695187166, Blast_Score=116, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.90 [H]

Molecular weight: Translated: 43783; Mature: 43783

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITEIRTRVVEWRGKTVPPQPHFCTNPMDILDLPADSMGSFRFHGWLIVEVFTDAGIVG
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHEECCCCCCCCEEECCEEEEEEECCCCEEE
IGNAALSPRVTKQLIDVYLKPLLLGKDPFDYEFLWQHMYRQTMAFGRKGVAMVAISALDI
ECCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
AIWDILGKAAKQPVFKLLGGRTKQKIPVYASRLYSQPLDQLAAEAKLYKDQGYRAMKLRF
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHCCCCEEEEEEE
GWGPVDGAAGMQRNVELVRTVREVVGSEIDVMADAYMGWTLDYARRMLPLLESFHLRWLE
CCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EPVIPDDIHGYAALKALGRIPIAGGEHEHTSYGFRELLEAKAVDYIQFDTNRVGGITQAR
CCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEECCCCCCCHHHHH
KVQSLAEAFSVPVVPHAGQMHNFHVVMASLNSPMAEFFPVVDVEIGNELFWYIFEGEPTP
HHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCHHHHCCEEEEEECCEEEEEEECCCCCC
MDGYIDLRDDVPGLGLTIKEDALGQFAIIE
CCCEEEECCCCCCCCEEEECCCCCCEEECC
>Mature Secondary Structure
MKITEIRTRVVEWRGKTVPPQPHFCTNPMDILDLPADSMGSFRFHGWLIVEVFTDAGIVG
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHEECCCCCCCCEEECCEEEEEEECCCCEEE
IGNAALSPRVTKQLIDVYLKPLLLGKDPFDYEFLWQHMYRQTMAFGRKGVAMVAISALDI
ECCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
AIWDILGKAAKQPVFKLLGGRTKQKIPVYASRLYSQPLDQLAAEAKLYKDQGYRAMKLRF
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHCCCCEEEEEEE
GWGPVDGAAGMQRNVELVRTVREVVGSEIDVMADAYMGWTLDYARRMLPLLESFHLRWLE
CCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EPVIPDDIHGYAALKALGRIPIAGGEHEHTSYGFRELLEAKAVDYIQFDTNRVGGITQAR
CCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEECCCCCCCHHHHH
KVQSLAEAFSVPVVPHAGQMHNFHVVMASLNSPMAEFFPVVDVEIGNELFWYIFEGEPTP
HHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCHHHHCCEEEEEECCEEEEEEECCCCCC
MDGYIDLRDDVPGLGLTIKEDALGQFAIIE
CCCEEEECCCCCCCCEEEECCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA