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The map label for this gene is rsmB [H]

Identifier: 116622296

GI number: 116622296

Start: 4039536

End: 4040618

Strand: Direct

Name: rsmB [H]

Synonym: Acid_3190

Alternate gene names: 116622296

Gene position: 4039536-4040618 (Clockwise)

Preceding gene: 116622295

Following gene: 116622297

Centisome position: 40.53

GC content: 66.76

Gene sequence:

>1083_bases
ATGCCTATAGCCGCTGCCCGCATCGCCGCGTTTGACATCCTTCAGCTGGTAGAGAGCGGCGGGTACGCGTCGGATTTGCT
GCTGGCGCGCACCGCGGATCTCGATTCGCGCGACGCCGGGCTCGCCTCCGAGATTGTTTTCGGAGTGCTGCGGTACCGGG
CGCAGCTCGATTTTCTGATCATTCACTACGCCGGACGCTCGCGGAAGCTCGATCCAGAGGTTCGCACCGCGCTGCGCATG
GCGATTTACCAGATTCGCTACCTCGAGCGGGTGCCGCCGCATGCGGCCGTGAAGGAAAGCGTGGAACTGGTGAAGCGGGC
ACGCAAAACATCCGCTGCCGGCTTCGTCAACGCCATTCTGCGGCAGGTGGACCGCGATCCGGTGATCTGGCCGGCGCGGG
AAATCGAATACTCCTGTCCGGAATGGCTGCTGGCGCGGTGGGAACGCGAGTACGGCGAGACGGCGGCGCTCGGAATCGCC
CAGGCGGCCCTGCGGGAGCCCGAGAAATACGTCCGGATCGGGACGGCCGGCGAACGCACGCAGGACATCGGGGCGCAATC
CATCGTGCCGCTGCTCGACCTCCAACCGGGACAGCGCTTCCTCGATCTCTGCGCGGCGCCGGGGAATAAGACGGCGCAGG
CGCTGGAGGCGGGCGTCCAGGCGGTGGCATGCGACCTTCACTTCCACCGGCTGGTGCAGCTCAAGCCGCTCACGCCGAAC
CTGGTGGTGGTGGACGGCACCCGGCCGCTGCCGTTCGCGGCGCGGTTCGACCGCATTCTGGTGGATGCGCCCTGCTCCGG
GACGGGGACGCTCGGCCGCAATCCGGAGATCAAGTGGCGGCTGACGCCGGCCGATCTCGCCGACCTGCACGCGCGCCAAT
CGGCCCTGCTGGCCAATGCCCTGAGCCTGCTCGCGCCGGGGGGGACTCTGGTCTATTCCACGTGCTCGCTGGAGCCCGAA
GAGAACGAAGGCGTCGCCGTTCAGGCGCGTGTGGAGCGGACCGAGCAGCGGCTTCCCGGCCGTGAAGCCGGGGACGGTTT
CTATGCTGCTGTGATAAAATGCGATATAGCCGTAAATGATTGA

Upstream 100 bases:

>100_bases
GGTTGAGGAGACGCGGCACTGGCGTTTGTATGTACTGTGCGGGGGCGTCGGCCGTCTTCAGGCGGGATTGCCTGCCCGCT
AACGGCTAGCATAGAGGGGA

Downstream 100 bases:

>100_bases
AATCGTTCCCTCCATCCTGTCTGCCGATTTCGCCAGACTGGCAGATGAAATCGCCCGCGTCGAGCGTGGCGGTGCGCGTA
TGCTGCACCTTGACGTGATG

Product: NusB/RsmB/TIM44

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MPIAAARIAAFDILQLVESGGYASDLLLARTADLDSRDAGLASEIVFGVLRYRAQLDFLIIHYAGRSRKLDPEVRTALRM
AIYQIRYLERVPPHAAVKESVELVKRARKTSAAGFVNAILRQVDRDPVIWPAREIEYSCPEWLLARWEREYGETAALGIA
QAALREPEKYVRIGTAGERTQDIGAQSIVPLLDLQPGQRFLDLCAAPGNKTAQALEAGVQAVACDLHFHRLVQLKPLTPN
LVVVDGTRPLPFAARFDRILVDAPCSGTGTLGRNPEIKWRLTPADLADLHARQSALLANALSLLAPGGTLVYSTCSLEPE
ENEGVAVQARVERTEQRLPGREAGDGFYAAVIKCDIAVND

Sequences:

>Translated_360_residues
MPIAAARIAAFDILQLVESGGYASDLLLARTADLDSRDAGLASEIVFGVLRYRAQLDFLIIHYAGRSRKLDPEVRTALRM
AIYQIRYLERVPPHAAVKESVELVKRARKTSAAGFVNAILRQVDRDPVIWPAREIEYSCPEWLLARWEREYGETAALGIA
QAALREPEKYVRIGTAGERTQDIGAQSIVPLLDLQPGQRFLDLCAAPGNKTAQALEAGVQAVACDLHFHRLVQLKPLTPN
LVVVDGTRPLPFAARFDRILVDAPCSGTGTLGRNPEIKWRLTPADLADLHARQSALLANALSLLAPGGTLVYSTCSLEPE
ENEGVAVQARVERTEQRLPGREAGDGFYAAVIKCDIAVND
>Mature_359_residues
PIAAARIAAFDILQLVESGGYASDLLLARTADLDSRDAGLASEIVFGVLRYRAQLDFLIIHYAGRSRKLDPEVRTALRMA
IYQIRYLERVPPHAAVKESVELVKRARKTSAAGFVNAILRQVDRDPVIWPAREIEYSCPEWLLARWEREYGETAALGIAQ
AALREPEKYVRIGTAGERTQDIGAQSIVPLLDLQPGQRFLDLCAAPGNKTAQALEAGVQAVACDLHFHRLVQLKPLTPNL
VVVDGTRPLPFAARFDRILVDAPCSGTGTLGRNPEIKWRLTPADLADLHARQSALLANALSLLAPGGTLVYSTCSLEPEE
NEGVAVQARVERTEQRLPGREAGDGFYAAVIKCDIAVND

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI39995082, Length=204, Percent_Identity=31.3725490196078, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI301336155, Length=204, Percent_Identity=31.3725490196078, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI32698918, Length=201, Percent_Identity=31.8407960199005, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI76150625, Length=172, Percent_Identity=34.8837209302326, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI76150623, Length=172, Percent_Identity=34.8837209302326, Blast_Score=79, Evalue=7e-15,
Organism=Escherichia coli, GI2367212, Length=424, Percent_Identity=33.4905660377358, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI87081985, Length=182, Percent_Identity=31.8681318681319, Blast_Score=76, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17536757, Length=160, Percent_Identity=40, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI25143471, Length=168, Percent_Identity=36.3095238095238, Blast_Score=88, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6319447, Length=194, Percent_Identity=39.1752577319588, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6324268, Length=162, Percent_Identity=34.5679012345679, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21355201, Length=176, Percent_Identity=38.0681818181818, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI22024126, Length=177, Percent_Identity=35.0282485875706, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19920494, Length=171, Percent_Identity=34.5029239766082, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24668781, Length=184, Percent_Identity=34.2391304347826, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI21356513, Length=184, Percent_Identity=34.2391304347826, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 39378; Mature: 39246

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIAAARIAAFDILQLVESGGYASDLLLARTADLDSRDAGLASEIVFGVLRYRAQLDFLI
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHEEEEE
IHYAGRSRKLDPEVRTALRMAIYQIRYLERVPPHAAVKESVELVKRARKTSAAGFVNAIL
EEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RQVDRDPVIWPAREIEYSCPEWLLARWEREYGETAALGIAQAALREPEKYVRIGTAGERT
HHCCCCCCCCCHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHEEEECCCCCHH
QDIGAQSIVPLLDLQPGQRFLDLCAAPGNKTAQALEAGVQAVACDLHFHRLVQLKPLTPN
HHCCHHHCCHHEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCC
LVVVDGTRPLPFAARFDRILVDAPCSGTGTLGRNPEIKWRLTPADLADLHARQSALLANA
EEEEECCCCCCHHHHHCEEEEECCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHH
LSLLAPGGTLVYSTCSLEPEENEGVAVQARVERTEQRLPGREAGDGFYAAVIKCDIAVND
HHHHCCCCEEEEEEECCCCCCCCCEEEEHHHHHHHHHCCCCCCCCCEEEEEEEEEEEECC
>Mature Secondary Structure 
PIAAARIAAFDILQLVESGGYASDLLLARTADLDSRDAGLASEIVFGVLRYRAQLDFLI
CCCHHHHHHHHHHHHHHCCCCHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHEEEEE
IHYAGRSRKLDPEVRTALRMAIYQIRYLERVPPHAAVKESVELVKRARKTSAAGFVNAIL
EEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RQVDRDPVIWPAREIEYSCPEWLLARWEREYGETAALGIAQAALREPEKYVRIGTAGERT
HHCCCCCCCCCHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHEEEECCCCCHH
QDIGAQSIVPLLDLQPGQRFLDLCAAPGNKTAQALEAGVQAVACDLHFHRLVQLKPLTPN
HHCCHHHCCHHEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCC
LVVVDGTRPLPFAARFDRILVDAPCSGTGTLGRNPEIKWRLTPADLADLHARQSALLANA
EEEEECCCCCCHHHHHCEEEEECCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHH
LSLLAPGGTLVYSTCSLEPEENEGVAVQARVERTEQRLPGREAGDGFYAAVIKCDIAVND
HHHHCCCCEEEEEEECCCCCCCCCEEEEHHHHHHHHHCCCCCCCCCEEEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA