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The map label for this gene is dkgB [H]

Identifier: 116621975

GI number: 116621975

Start: 3620930

End: 3621880

Strand: Reverse

Name: dkgB [H]

Synonym: Acid_2860

Alternate gene names: 116621975

Gene position: 3621880-3620930 (Counterclockwise)

Preceding gene: 116621979

Following gene: 116621974

Centisome position: 36.34

GC content: 62.15

Gene sequence:

>951_bases
ATGCCGGCGATCGGGCTCGGCACGTTTGGAAGCGACCACGTTTCGGCGGAGCAGATTGCCGACGTGGTGCGCGACGCAGC
GGTTGTGGGCTACCGGCATTTTGATTGCGCGAGCGTCTACCGCAATGAAGACCGGATCGGGTTCGCGTTTCGGGACATCA
TGAAGGGCGGGATCCGGCGGGAGGATTTGTGGATCACGAGCAAGCTGTGGAACGATAAGCACGGCGAAGGGGACGTGATC
GCGTCATGCAGGCAATCGCTGGCCGATCTTCAGCTCGATTATCTGGACATGTACCTGGTGCACTGGCCGTTTCCCAACTT
CCACCCACCGGGGTGCGATGTCAGTTCGCGGAGCCCGGACGCGCGTCCGTATATCCACGAGAATTACATGAAGACCTGGC
GGGAGATGGAGAGGCTGGTGGAGCTAGGGCTGGTGCGGCATATCGGCACCAGCAATGTGACGATCCCGAAGCTGAAGCTG
ATCCTGCGCGACGCGCGGATCCGGCCGGCGGTCAGCGAGATGGAGATGCATCCGCACTTTCAGCAGAGGGAACTGTTCGA
GTTTGTGCGCGCGGCAGGGATCGAGCCGATCGCGTACAGCCCGATCGGCTCGCCCGGGCGGCCCGAGCGCGACCGGACGC
CGGAGGATACGGTGGATATCGAAGATCCGGTGATCGTCGCGATTGCGAAGCGGATCGGGGTCCATCCAGCGGTGGTCTGC
GTGAAGTGGGCGGAGCAACTGGGCGCGACGCCGATTCCGTTTTCGACGCGGCGGCGGAACTACCTTGCGAACCTGCAGGG
CGTGGTGAGCGACCCGCTGACCGCGGCCGATATGGAGGCTATCGCCGGAATCGATCGCAACTGCCGGCTGATTAAAGGGC
AGGTATTTTTGTGGAAACAGGACCAGACGTGGGAAGATCTCTGGGACCTGAAGGGAGTGATCGCACAATGA

Upstream 100 bases:

>100_bases
TCCCTTAGAGGCGGGCAGTAAACGATAAAGGTGGGGGAATTTGGCTGTAATATAGAGGTTCATGAACGTTCCTCAGCGGA
CACTTTATACAGGAGCACGG

Downstream 100 bases:

>100_bases
TTGGGGCCTATCTGCCGGGGAATAGCACGGTCGAGATGAAGGAAGTGCCGGTGCCGGTTCCGGCACACGGCGAGGTTCTG
CTGCGCATGAAGGCGTCAAC

Product: aldehyde reductase

Products: NA

Alternate protein names: 2,5-DKG reductase B; 2,5-DKGR B; 25DKGR-B; AKR5D [H]

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MPAIGLGTFGSDHVSAEQIADVVRDAAVVGYRHFDCASVYRNEDRIGFAFRDIMKGGIRREDLWITSKLWNDKHGEGDVI
ASCRQSLADLQLDYLDMYLVHWPFPNFHPPGCDVSSRSPDARPYIHENYMKTWREMERLVELGLVRHIGTSNVTIPKLKL
ILRDARIRPAVSEMEMHPHFQQRELFEFVRAAGIEPIAYSPIGSPGRPERDRTPEDTVDIEDPVIVAIAKRIGVHPAVVC
VKWAEQLGATPIPFSTRRRNYLANLQGVVSDPLTAADMEAIAGIDRNCRLIKGQVFLWKQDQTWEDLWDLKGVIAQ

Sequences:

>Translated_316_residues
MPAIGLGTFGSDHVSAEQIADVVRDAAVVGYRHFDCASVYRNEDRIGFAFRDIMKGGIRREDLWITSKLWNDKHGEGDVI
ASCRQSLADLQLDYLDMYLVHWPFPNFHPPGCDVSSRSPDARPYIHENYMKTWREMERLVELGLVRHIGTSNVTIPKLKL
ILRDARIRPAVSEMEMHPHFQQRELFEFVRAAGIEPIAYSPIGSPGRPERDRTPEDTVDIEDPVIVAIAKRIGVHPAVVC
VKWAEQLGATPIPFSTRRRNYLANLQGVVSDPLTAADMEAIAGIDRNCRLIKGQVFLWKQDQTWEDLWDLKGVIAQ
>Mature_315_residues
PAIGLGTFGSDHVSAEQIADVVRDAAVVGYRHFDCASVYRNEDRIGFAFRDIMKGGIRREDLWITSKLWNDKHGEGDVIA
SCRQSLADLQLDYLDMYLVHWPFPNFHPPGCDVSSRSPDARPYIHENYMKTWREMERLVELGLVRHIGTSNVTIPKLKLI
LRDARIRPAVSEMEMHPHFQQRELFEFVRAAGIEPIAYSPIGSPGRPERDRTPEDTVDIEDPVIVAIAKRIGVHPAVVCV
KWAEQLGATPIPFSTRRRNYLANLQGVVSDPLTAADMEAIAGIDRNCRLIKGQVFLWKQDQTWEDLWDLKGVIAQ

Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) [H]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI24497577, Length=293, Percent_Identity=39.2491467576792, Blast_Score=200, Evalue=1e-51,
Organism=Homo sapiens, GI5174391, Length=293, Percent_Identity=39.2491467576792, Blast_Score=200, Evalue=1e-51,
Organism=Homo sapiens, GI45446745, Length=312, Percent_Identity=33.6538461538462, Blast_Score=178, Evalue=5e-45,
Organism=Homo sapiens, GI4503285, Length=312, Percent_Identity=33.6538461538462, Blast_Score=178, Evalue=5e-45,
Organism=Homo sapiens, GI310109920, Length=300, Percent_Identity=34.3333333333333, Blast_Score=178, Evalue=6e-45,
Organism=Homo sapiens, GI5453543, Length=300, Percent_Identity=34, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI24497583, Length=312, Percent_Identity=33.0128205128205, Blast_Score=172, Evalue=3e-43,
Organism=Homo sapiens, GI93277124, Length=313, Percent_Identity=34.8242811501597, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI310109922, Length=311, Percent_Identity=31.5112540192926, Blast_Score=167, Evalue=9e-42,
Organism=Homo sapiens, GI24497585, Length=312, Percent_Identity=31.0897435897436, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI4502049, Length=295, Percent_Identity=37.9661016949153, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI223468663, Length=293, Percent_Identity=34.4709897610922, Blast_Score=158, Evalue=6e-39,
Organism=Homo sapiens, GI5174695, Length=304, Percent_Identity=30.5921052631579, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI291291012, Length=295, Percent_Identity=32.2033898305085, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI300116273, Length=271, Percent_Identity=31.7343173431734, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI300116271, Length=302, Percent_Identity=26.8211920529801, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI207028673, Length=104, Percent_Identity=41.3461538461538, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI310109926, Length=104, Percent_Identity=40.3846153846154, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI310109924, Length=175, Percent_Identity=32, Blast_Score=92, Evalue=5e-19,
Organism=Escherichia coli, GI87082198, Length=290, Percent_Identity=30, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1786400, Length=292, Percent_Identity=32.8767123287671, Blast_Score=135, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17537075, Length=293, Percent_Identity=34.4709897610922, Blast_Score=177, Evalue=7e-45,
Organism=Caenorhabditis elegans, GI17537077, Length=293, Percent_Identity=34.8122866894198, Blast_Score=175, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17552492, Length=286, Percent_Identity=31.1188811188811, Blast_Score=140, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI17564128, Length=296, Percent_Identity=30.0675675675676, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17537079, Length=297, Percent_Identity=29.2929292929293, Blast_Score=134, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17550248, Length=300, Percent_Identity=29.3333333333333, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17566692, Length=266, Percent_Identity=30.4511278195489, Blast_Score=128, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI71998625, Length=291, Percent_Identity=30.5841924398625, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17561298, Length=305, Percent_Identity=29.1803278688525, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17561300, Length=271, Percent_Identity=29.8892988929889, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17562292, Length=291, Percent_Identity=27.4914089347079, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17538386, Length=206, Percent_Identity=31.0679611650485, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324694, Length=298, Percent_Identity=35.2348993288591, Blast_Score=159, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6321896, Length=305, Percent_Identity=32.1311475409836, Blast_Score=155, Evalue=8e-39,
Organism=Saccharomyces cerevisiae, GI6320576, Length=302, Percent_Identity=29.4701986754967, Blast_Score=138, Evalue=9e-34,
Organism=Saccharomyces cerevisiae, GI6319625, Length=267, Percent_Identity=31.8352059925094, Blast_Score=125, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6322556, Length=271, Percent_Identity=26.5682656826568, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6320079, Length=285, Percent_Identity=24.2105263157895, Blast_Score=86, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24662789, Length=295, Percent_Identity=35.5932203389831, Blast_Score=174, Evalue=7e-44,
Organism=Drosophila melanogaster, GI24644950, Length=304, Percent_Identity=34.8684210526316, Blast_Score=163, Evalue=1e-40,
Organism=Drosophila melanogaster, GI20129731, Length=260, Percent_Identity=38.0769230769231, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI21356425, Length=295, Percent_Identity=34.5762711864407, Blast_Score=160, Evalue=8e-40,
Organism=Drosophila melanogaster, GI24663317, Length=293, Percent_Identity=32.7645051194539, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24657054, Length=259, Percent_Identity=35.5212355212355, Blast_Score=158, Evalue=4e-39,
Organism=Drosophila melanogaster, GI221378297, Length=324, Percent_Identity=33.3333333333333, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24662785, Length=299, Percent_Identity=37.123745819398, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24662781, Length=299, Percent_Identity=37.123745819398, Blast_Score=155, Evalue=2e-38,
Organism=Drosophila melanogaster, GI45553081, Length=275, Percent_Identity=34.5454545454545, Blast_Score=145, Evalue=4e-35,
Organism=Drosophila melanogaster, GI281366140, Length=247, Percent_Identity=34.8178137651822, Blast_Score=135, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: =1.1.1.274 [H]

Molecular weight: Translated: 35852; Mature: 35721

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAIGLGTFGSDHVSAEQIADVVRDAAVVGYRHFDCASVYRNEDRIGFAFRDIMKGGIRR
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHCCCCC
EDLWITSKLWNDKHGEGDVIASCRQSLADLQLDYLDMYLVHWPFPNFHPPGCDVSSRSPD
HHEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHEEHEECCCCCCCCCCCCCCCCCCC
ARPYIHENYMKTWREMERLVELGLVRHIGTSNVTIPKLKLILRDARIRPAVSEMEMHPHF
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHCCCCH
QQRELFEFVRAAGIEPIAYSPIGSPGRPERDRTPEDTVDIEDPVIVAIAKRIGVHPAVVC
HHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHH
VKWAEQLGATPIPFSTRRRNYLANLQGVVSDPLTAADMEAIAGIDRNCRLIKGQVFLWKQ
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEEECEEEEEEC
DQTWEDLWDLKGVIAQ
CCCHHHHHHHHHHHCC
>Mature Secondary Structure 
PAIGLGTFGSDHVSAEQIADVVRDAAVVGYRHFDCASVYRNEDRIGFAFRDIMKGGIRR
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHCCCCC
EDLWITSKLWNDKHGEGDVIASCRQSLADLQLDYLDMYLVHWPFPNFHPPGCDVSSRSPD
HHEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHEEHEECCCCCCCCCCCCCCCCCCC
ARPYIHENYMKTWREMERLVELGLVRHIGTSNVTIPKLKLILRDARIRPAVSEMEMHPHF
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHCCCCH
QQRELFEFVRAAGIEPIAYSPIGSPGRPERDRTPEDTVDIEDPVIVAIAKRIGVHPAVVC
HHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHH
VKWAEQLGATPIPFSTRRRNYLANLQGVVSDPLTAADMEAIAGIDRNCRLIKGQVFLWKQ
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEEECEEEEEEC
DQTWEDLWDLKGVIAQ
CCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]