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The map label for this gene is ciaH

Identifier: 116516689

GI number: 116516689

Start: 715487

End: 716821

Strand: Direct

Name: ciaH

Synonym: SPD_0702

Alternate gene names: 116516689

Gene position: 715487-716821 (Clockwise)

Preceding gene: 116515465

Following gene: 116516394

Centisome position: 34.97

GC content: 38.5

Gene sequence:

>1335_bases
ATGTTCAGTAAACTTAAAAAAACATGGTATGCGGATGACTTTAGTTATTTTATCCGCAACTTCGGTGTCTTCACCCTGAT
TTTTTCTACAATGACTCTGATTATTTTACAAGTCATGCATTCGAGTCTTTATACTTCGGTGGACGATAAGCTTCATGGAC
TGAGTGAAAATCCTCAAGCAGTTATTCAGCTGGCTATAAATAGGGCAACAGAAGAGATTAAAGATTTAGAAAATGCTAGG
GCGGACGCTAGTAAAGTAGAAATAAAACCTAATGTCAGTTCCAATACGGAAGTCATTCTCTTTGATAAGGACTTTACTCA
ACTTCTTTCTGGAAATCGATTTTTGGGCTTGGATAAGATTAAGTTAGAAAAGAAAGAACTAGGACATATCTACCAGATTC
AGGTTTTTAATAGCTATGGGCAGGAAGAAATCTATCGTGTGATTTTGATGGAGACCAATATTAGTTCGGTTTCAACCAAT
ATCAAGTATGCTGCTGTCTTGATTAATACCAGTCAGTTGGAGCAGGCTAGTCAAAAGCATGAGCAATTGATTGTGGTCGT
GATGGCTAGTTTCTGGATTTTGTCTTTACTTGCCAGTCTCTATCTAGCTAGGGTCAGTGTTAGGCCCCTGCTTGAGAGTA
TGCAGAAGCAACAGTCTTTTGTGGAAAATGCCAGTCATGAGTTACGAACTCCACTCGCAGTTTTGCAAAATCGCTTAGAG
ACCCTTTTTCGTAAGCCAGAAGCTACCATTATGGATGTGAGCGAAAGCATTGCATCGAGTTTGGAAGAAGTCCGAAATAT
GCGTTTTTTAACGACAAGCTTGCTGAACTTAGCTCGGAGAGATGATGGGATTAAGCCGGAGCTTGCAGAAGTTCCAACTA
GCTTTTTTAATACAACTTTCACAAACTATGAGATGATTGCTTCGGAAAATAATCGTGTCTTCCGTTTTGAAAATCGTATC
CATCGAACAATTGTCACAGATCAGCTTCTTCTGAAACAACTGATGACCATTCTTTTCGATAATGCCGTCAAGTATACTGA
GGAGGATGGTGAAATTGATTTTCTTATCTCGGCGACCGATCGCAATCTTTATTTACTTGTTTCTGATAATGGAATCGGTA
TTTCGACAGAAGATAAAAAGAAAATTTTTGACCGTTTTTATCGAGTAGACAAGGCTAGAACCCGGCAAAAAGGTGGTTTT
GGTTTAGGATTATCCCTAGCCAAGCAAATTGTAGATGCTCTAAAAGGAACTGTTACTGTCAAAGATAATAAACCCAAGGG
AACAATCTTTGAAGTGAAGATTGCCATTCAGACACCATCTAAAAAGAAAAAATAA

Upstream 100 bases:

>100_bases
GTTGTCGAAGTCTATGTTTCAAAAGTCCGTAAGAAATTAAAGGGAACCACTTTTGCAGAGAATTTGCAAACCTTGCGTAG
TGTTGGGTATCTTTTAAAAG

Downstream 100 bases:

>100_bases
AAATATCGCTCCAATTGGGGCGATATTTTGGATTTATCTTCTACGTTTTCGTTTGATAATAGACCGTTGAACTTTTAAAA
CAAGTAAGCTGAATCCGATT

Product: sensor histidine kinase CiaH

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLYTSVDDKLHGLSENPQAVIQLAINRATEEIKDLENAR
ADASKVEIKPNVSSNTEVILFDKDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQSFVENASHELRTPLAVLQNRLE
TLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLNLARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRI
HRTIVTDQLLLKQLMTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKARTRQKGGF
GLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK

Sequences:

>Translated_444_residues
MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLYTSVDDKLHGLSENPQAVIQLAINRATEEIKDLENAR
ADASKVEIKPNVSSNTEVILFDKDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQSFVENASHELRTPLAVLQNRLE
TLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLNLARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRI
HRTIVTDQLLLKQLMTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKARTRQKGGF
GLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK
>Mature_444_residues
MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLYTSVDDKLHGLSENPQAVIQLAINRATEEIKDLENAR
ADASKVEIKPNVSSNTEVILFDKDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQSFVENASHELRTPLAVLQNRLE
TLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLNLARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRI
HRTIVTDQLLLKQLMTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKARTRQKGGF
GLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK

Specific function: Member of the two-component regulatory system CiaH/CiaR. Involved in early steps of competence regulation and in penicillin susceptibility. Probably phosphorylates CiaR

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain

Homologues:

Organism=Escherichia coli, GI1786600, Length=231, Percent_Identity=31.6017316017316, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1788393, Length=239, Percent_Identity=30.1255230125523, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1786783, Length=226, Percent_Identity=29.646017699115, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788279, Length=345, Percent_Identity=24.9275362318841, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787894, Length=219, Percent_Identity=28.310502283105, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1789149, Length=230, Percent_Identity=27.3913043478261, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788713, Length=227, Percent_Identity=24.6696035242291, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1786912, Length=287, Percent_Identity=28.5714285714286, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI145693157, Length=245, Percent_Identity=26.530612244898, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI87082128, Length=233, Percent_Identity=25.7510729613734, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787374, Length=240, Percent_Identity=28.75, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1790346, Length=235, Percent_Identity=26.8085106382979, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790861, Length=229, Percent_Identity=24.0174672489083, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI48994928, Length=239, Percent_Identity=26.7782426778243, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CIAH_STRPN (P0A4I5)

Other databases:

- EMBL:   AE005672
- PIR:   G95092
- RefSeq:   NP_345296.1
- ProteinModelPortal:   P0A4I5
- EnsemblBacteria:   EBSTRT00000026371
- GeneID:   930749
- GenomeReviews:   AE005672_GR
- KEGG:   spn:SP_0799
- TIGR:   SP_0799
- GeneTree:   EBGT00050000026967
- HOGENOM:   HBG532068
- OMA:   IVFWLIS
- ProtClustDB:   CLSK876906
- BioCyc:   SPNE170187-1:SP_0799-MONOMER
- BRENDA:   2.7.13.3
- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- Gene3D:   G3DSA:3.30.565.10
- PRINTS:   PR00344
- SMART:   SM00387
- SMART:   SM00388

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; SSF55874 ATP_bd_ATPase; SSF47384 His_kin_homodim

EC number: =2.7.13.3

Molecular weight: Translated: 50674; Mature: 50674

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1ae4beec)-; HASH(0x1b059128)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLYTSVDDKLHGLSENPQA
CCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
VIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFDKDFTQLLSGNRFLGLDKI
HHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEECCHHHHHHCCCCEECCHHH
KLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTNIKYAAVLINTSQLEQASQKH
EECHHHCCCEEEEEEECCCCHHHHEEEEEEECCCHHHHCCCEEEEEEEEHHHHHHHHHHH
EQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQSFVENASHELRTPLAVLQNRLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
TLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLNLARRDDGIKPELAEVPTSFFNTTF
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHCCC
TNYEMIASENNRVFRFENRIHRTIVTDQLLLKQLMTILFDNAVKYTEEDGEIDFLISATD
CCEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCEEEEEEECC
RNLYLLVSDNGIGISTEDKKKIFDRFYRVDKARTRQKGGFGLGLSLAKQIVDALKGTVTV
CEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCEEEE
KDNKPKGTIFEVKIAIQTPSKKKK
ECCCCCCCEEEEEEEEECCCCCCH
>Mature Secondary Structure
MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLYTSVDDKLHGLSENPQA
CCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
VIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFDKDFTQLLSGNRFLGLDKI
HHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEECCHHHHHHCCCCEECCHHH
KLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTNIKYAAVLINTSQLEQASQKH
EECHHHCCCEEEEEEECCCCHHHHEEEEEEECCCHHHHCCCEEEEEEEEHHHHHHHHHHH
EQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQSFVENASHELRTPLAVLQNRLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
TLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLNLARRDDGIKPELAEVPTSFFNTTF
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHCCC
TNYEMIASENNRVFRFENRIHRTIVTDQLLLKQLMTILFDNAVKYTEEDGEIDFLISATD
CCEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCEEEEEEECC
RNLYLLVSDNGIGISTEDKKKIFDRFYRVDKARTRQKGGFGLGLSLAKQIVDALKGTVTV
CEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCEEEE
KDNKPKGTIFEVKIAIQTPSKKKK
ECCCCCCCEEEEEEEEECCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11463916