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The map label for this gene is ciaR [H]

Identifier: 116515465

GI number: 116515465

Start: 714757

End: 715497

Strand: Direct

Name: ciaR [H]

Synonym: SPD_0701

Alternate gene names: 116515465

Gene position: 714757-715497 (Clockwise)

Preceding gene: 116515610

Following gene: 116516689

Centisome position: 34.93

GC content: 37.25

Gene sequence:

>741_bases
ATGCAGAGAGTTGAGTTTTTTTTAAGGCAAATTTGGTATAATAGTTTTAATAAGGAGGAGTTTCTCATGATAAAAATCTT
ATTGGTTGAGGATGACCTAGGTCTGTCAAATTCAGTATTTGACTTTTTAGACGATTTTGCGGATGTTATGCAGGTATTTG
ATGGAGAAGAAGGTCTCTACGAAGCTGAGAGTGGTGTCTATGACTTGATTTTGCTGGATTTGATGTTGCCAGAAAAAAAT
GGTTTCCAAGTCTTAAAAGAATTGCGTGAAAAGGGAATTACGACACCAGTTCTGATTATGACTGCCAAGGAAAGTTTGGA
TGACAAGGGACATGGATTTGAACTGGGAGCGGATGATTATCTGACCAAACCTTTCTACCTAGAAGAACTTAAAATGCGGA
TTCAGGCCCTTCTCAAACGTTCAGGGAAGTTTAATGAAAACACCTTGACTTATGGGAATATCGTGGTTAATTTATCAACC
AATACCGTTAAAGTTGAAGATACTCCTGTCGAATTGCTGGGGAAAGAGTTCGATTTACTAGTTTATTTCCTTCAAAATCA
AAATGTGATTTTGCCTAAGACGCAGATTTTTGACCGTCTATGGGGATTTGATAGTGATACAACGATTTCGGTTGTCGAAG
TCTATGTTTCAAAAGTCCGTAAGAAATTAAAGGGAACCACTTTTGCAGAGAATTTGCAAACCTTGCGTAGTGTTGGGTAT
CTTTTAAAAGATGTTCAGTAA

Upstream 100 bases:

>100_bases
GATTAGCCGTGAAAAAGCTGCAGTCGAAGCAGTTGTTCTTCAATACGGAAATGCATAAATAAGCCTAAAATAAAAAGAAA
ACTCAGCTATCTCATGTAAA

Downstream 100 bases:

>100_bases
ACTTAAAAAAACATGGTATGCGGATGACTTTAGTTATTTTATCCGCAACTTCGGTGTCTTCACCCTGATTTTTTCTACAA
TGACTCTGATTATTTTACAA

Product: DNA-binding response regulator CiaR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MQRVEFFLRQIWYNSFNKEEFLMIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGVYDLILLDLMLPEKN
GFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKPFYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLST
NTVKVEDTPVELLGKEFDLLVYFLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
LLKDVQ

Sequences:

>Translated_246_residues
MQRVEFFLRQIWYNSFNKEEFLMIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGVYDLILLDLMLPEKN
GFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKPFYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLST
NTVKVEDTPVELLGKEFDLLVYFLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
LLKDVQ
>Mature_246_residues
MQRVEFFLRQIWYNSFNKEEFLMIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGVYDLILLDLMLPEKN
GFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKPFYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLST
NTVKVEDTPVELLGKEFDLLVYFLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
LLKDVQ

Specific function: Member of the two-component regulatory system CiaH/CiaR. Involved in early steps of competence regulation and in penicillin susceptibility [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789402, Length=223, Percent_Identity=36.322869955157, Blast_Score=143, Evalue=9e-36,
Organism=Escherichia coli, GI1786784, Length=229, Percent_Identity=34.9344978165939, Blast_Score=137, Evalue=6e-34,
Organism=Escherichia coli, GI87082012, Length=219, Percent_Identity=36.0730593607306, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1790552, Length=225, Percent_Identity=33.7777777777778, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1786599, Length=220, Percent_Identity=30.4545454545455, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1787375, Length=219, Percent_Identity=27.3972602739726, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1786911, Length=207, Percent_Identity=30.4347826086957, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1790860, Length=222, Percent_Identity=30.6306306306306, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI1789809, Length=228, Percent_Identity=29.3859649122807, Blast_Score=97, Evalue=7e-22,
Organism=Escherichia coli, GI2367329, Length=230, Percent_Identity=35.2173913043478, Blast_Score=97, Evalue=8e-22,
Organism=Escherichia coli, GI1787229, Length=227, Percent_Identity=29.0748898678414, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1790863, Length=226, Percent_Identity=28.3185840707965, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788394, Length=221, Percent_Identity=25.7918552036199, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI145693140, Length=240, Percent_Identity=28.3333333333333, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790437, Length=126, Percent_Identity=30.1587301587302, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788550, Length=72, Percent_Identity=38.8888888888889, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 28374; Mature: 28374

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRVEFFLRQIWYNSFNKEEFLMIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLY
CCHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCH
EAESGVYDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDY
HHCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEEHHHCCCCCCCEECCCCCC
LTKPFYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLL
CCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCEEEECCCCHHHHCCHHHEE
VYFLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
EEEECCCCEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LLKDVQ
HHHCCC
>Mature Secondary Structure
MQRVEFFLRQIWYNSFNKEEFLMIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLY
CCHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCH
EAESGVYDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDY
HHCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEEHHHCCCCCCCEECCCCCC
LTKPFYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLL
CCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEECCCEEEECCCCHHHHCCHHHEE
VYFLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
EEEECCCCEECCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LLKDVQ
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11463916 [H]