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The map label for this gene is trpB

Identifier: 116516622

GI number: 116516622

Start: 1612842

End: 1614065

Strand: Reverse

Name: trpB

Synonym: SPD_1597

Alternate gene names: 116516622

Gene position: 1614065-1612842 (Counterclockwise)

Preceding gene: 116515967

Following gene: 116515328

Centisome position: 78.88

GC content: 48.53

Gene sequence:

>1224_bases
ATGGCATATCAAGAACCAAATAAAGATGGATTTTACGGAAAATTTGGCGGACGTTTTGTCCCAGAAACATTGATGACAGC
AGTTTTGGAGTTGGAGAAGGCCTACCGTGAAAGTCAGGCAGACCCAAGTTTCCAAGAGGAATTAAACCAACTTTTGCGCC
AGTATGTGGGACGTGAAACTCCCCTTTACTACGCAAAAAACTTGACCCAGCATATCGGCGGAGCCAAGATTTATCTCAAA
CGGGAAGACCTTAACCATACAGGAGCCCACAAGATTAACAATGCCTTAGGACAAGTTTGGCTTGCCAAACGCATGGGTAA
AAAGAAAATTATCGCAGAAACGGGTGCTGGTCAGCACGGTGTGGCAACTGCAACTGCTGCAGCCCTCTTTAATATGGAAT
GTATCATCTACATGGGTGAGGAAGATGTCAAACGCCAAGCTCTCAATGTCTTCCGTATGGAGCTTTTGGGAGCTAAGGTC
GAGGCCGTGACAGATGGTTCGCGCGTGCTCAAGGATGCGGTCAATGCAGCCCTTCGTTCATGGGTAGCTAATATCGATGA
TACCCACTATATCCTTGGTTCTACCTTGGGGCCTCATCCATTTCCAGAAATCGTTCGTGACTTCCAAAGTGTCATCGGTC
GAGAGGCTAAACAACAGTACCGTGACTTGACAGGACAAAATCTGCCAGATGCCCTAGTAGCCTGTGTTGGTGGTGGGTCG
AATGCCATCGGTCTCTTCCATCCCTTTGTAGAAGATGAGTCTGTAGCTATGTATGGGGCTGAAGCAGCTGGACTTGGTGT
GGATACGGAGCACCACGCAGCTACCTTGACCAAGGGTCGTCCAGGTGTCCTTCACGGTTCCCTCATGGATGTGCTCCAAG
ATGCCCATGGTCAAATTCTTGAAGCCTTCTCTATCTCAGCAGGTTTGGACTATCCTGGTATCGGTCCAGAGCATTCTCAC
TACCACGATATTAAACGTGCCAGCTATGTCCCTGTGACAGACGAAGAAGCCTTGGAAGGATTCCAACTCTTGTCTCGTGT
GGAAGGGATTATCCCAGCCTTGGAATCTAGCCATGCTATCGCTTTTGCGGTGAAATTGGCCAAAGAACTTGGACCAGAAA
AGTCTATGATTGTCTGCCTATCAGGTCGTGGGGACAAGGATGTGGTTCAAGTCAAAGACCGCTTGGAAGCAGATGCAGCA
AAGAAGGGAGAAGCTCATGCCTAA

Upstream 100 bases:

>100_bases
CAACACTTTACTCCCTATGCAGTAGATGTATCAAGCGGAGTGGAGACAGATGGACAAAAAGATCATGAAAAGATTAGAAG
ATTTATAGAGAGGGTAAAGA

Downstream 100 bases:

>100_bases
GACACTAACAGAAAAATTGAATGCTATAAAAGCAACTGGAAAAGGAATTTTTGTTCCCTATATCATGGCTGGTGACCACG
AGAAAGGTTTGGATGGACTA

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSFQEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLK
REDLNHTGAHKINNALGQVWLAKRMGKKKIIAETGAGQHGVATATAAALFNMECIIYMGEEDVKRQALNVFRMELLGAKV
EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSTLGPHPFPEIVRDFQSVIGREAKQQYRDLTGQNLPDALVACVGGGS
NAIGLFHPFVEDESVAMYGAEAAGLGVDTEHHAATLTKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSH
YHDIKRASYVPVTDEEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVKDRLEADAA
KKGEAHA

Sequences:

>Translated_407_residues
MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSFQEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLK
REDLNHTGAHKINNALGQVWLAKRMGKKKIIAETGAGQHGVATATAAALFNMECIIYMGEEDVKRQALNVFRMELLGAKV
EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSTLGPHPFPEIVRDFQSVIGREAKQQYRDLTGQNLPDALVACVGGGS
NAIGLFHPFVEDESVAMYGAEAAGLGVDTEHHAATLTKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSH
YHDIKRASYVPVTDEEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVKDRLEADAA
KKGEAHA
>Mature_406_residues
AYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSFQEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKR
EDLNHTGAHKINNALGQVWLAKRMGKKKIIAETGAGQHGVATATAAALFNMECIIYMGEEDVKRQALNVFRMELLGAKVE
AVTDGSRVLKDAVNAALRSWVANIDDTHYILGSTLGPHPFPEIVRDFQSVIGREAKQQYRDLTGQNLPDALVACVGGGSN
AIGLFHPFVEDESVAMYGAEAAGLGVDTEHHAATLTKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHY
HDIKRASYVPVTDEEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVKDRLEADAAK
KGEAHA

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family

Homologues:

Organism=Escherichia coli, GI1787515, Length=389, Percent_Identity=52.9562982005141, Blast_Score=411, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6321412, Length=392, Percent_Identity=57.1428571428571, Blast_Score=473, Evalue=1e-134,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPB_STRP2 (Q04IY9)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_817049.1
- ProteinModelPortal:   Q04IY9
- SMR:   Q04IY9
- STRING:   Q04IY9
- EnsemblBacteria:   EBSTRT00000020572
- GeneID:   4442400
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_1597
- eggNOG:   COG0133
- GeneTree:   EBGT00050000028106
- HOGENOM:   HBG303148
- OMA:   LKREDLC
- ProtClustDB:   PRK04346
- HAMAP:   MF_00133
- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026
- TIGRFAMs:   TIGR00263

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.2.1.20

Molecular weight: Translated: 44259; Mature: 44128

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSFQEELNQLLRQYVGRET
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCC
PLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLAKRMGKKKIIAETGAGQHG
CHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCC
VATATAAALFNMECIIYMGEEDVKRQALNVFRMELLGAKVEAVTDGSRVLKDAVNAALRS
HHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHH
WVANIDDTHYILGSTLGPHPFPEIVRDFQSVIGREAKQQYRDLTGQNLPDALVACVGGGS
HHHCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCC
NAIGLFHPFVEDESVAMYGAEAAGLGVDTEHHAATLTKGRPGVLHGSLMDVLQDAHGQIL
CCEEHCCCCCCCCCEEEECCCCCCCCCCCCHHHHEECCCCCCCCHHHHHHHHHHHHHHHH
EAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTDEEALEGFQLLSRVEGIIPALESSHAI
HHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHH
AFAVKLAKELGPEKSMIVCLSGRGDKDVVQVKDRLEADAAKKGEAHA
HHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSFQEELNQLLRQYVGRET
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCC
PLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLAKRMGKKKIIAETGAGQHG
CHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCC
VATATAAALFNMECIIYMGEEDVKRQALNVFRMELLGAKVEAVTDGSRVLKDAVNAALRS
HHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHH
WVANIDDTHYILGSTLGPHPFPEIVRDFQSVIGREAKQQYRDLTGQNLPDALVACVGGGS
HHHCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCC
NAIGLFHPFVEDESVAMYGAEAAGLGVDTEHHAATLTKGRPGVLHGSLMDVLQDAHGQIL
CCEEHCCCCCCCCCEEEECCCCCCCCCCCCHHHHEECCCCCCCCHHHHHHHHHHHHHHHH
EAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTDEEALEGFQLLSRVEGIIPALESSHAI
HHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHH
AFAVKLAKELGPEKSMIVCLSGRGDKDVVQVKDRLEADAAKKGEAHA
HHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA