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The map label for this gene is trpF

Identifier: 116515967

GI number: 116515967

Start: 1614043

End: 1614642

Strand: Reverse

Name: trpF

Synonym: SPD_1598

Alternate gene names: 116515967

Gene position: 1614642-1614043 (Counterclockwise)

Preceding gene: 116516838

Following gene: 116516622

Centisome position: 78.91

GC content: 43.5

Gene sequence:

>600_bases
TTGACAAAGGTTAAAATTTGTGGACTATCGACCAAAGAAGCGGTGGAAACAGCCGTTTCAGCAGGAGCCGACTATATCGG
TTTTGTCTTTGCACCTAGTAAAAGACAGGTGACTTTAGAAGAGGCAGCTGAGTTGGCAAAGCTTATTCCTGCAGATGTGA
AAAAGGTTGGAGTATTTGTTTCACCAAGTCGGGTAGAACTGCTGGAAGCGATTGACAAAGTTGGCTTGGACTTGGTTCAA
GTTCACGGTCAGGTAGCAGATGATTTATTTGAGAATTTGCCTTGTGCCAGTATTCAGGCTGTGCAGGTAGATGGAAATGG
GCATGTCCCTAATTCTCAGGCAGATTATCTACTCTTTGATGCCCCTGTGGCAGGAAGTGGCCAGCCCTTTGATTGGGGTC
AACTGGATACGACTGGACTAGCACAGCCCTTCTTTATCGCAGGTGGCCTTAATGAAGATAATGTAGTAAAAGCAATTCAA
CACTTTACTCCCTATGCAGTAGATGTATCAAGCGGAGTGGAGACAGATGGACAAAAAGATCATGAAAAGATTAGAAGATT
TATAGAGAGGGTAAAGAATGGCATATCAAGAACCAAATAA

Upstream 100 bases:

>100_bases
CAGGATGCGGAACGACTAGCCCCATACTTTAATGGAATTTTGGTAGGGACAGCTCTTATGCAGGCAGAAAATGTGGTCCA
GAGAATCAAGGAGTTGCAGA

Downstream 100 bases:

>100_bases
AGATGGATTTTACGGAAAATTTGGCGGACGTTTTGTCCCAGAAACATTGATGACAGCAGTTTTGGAGTTGGAGAAGGCCT
ACCGTGAAAGTCAGGCAGAC

Product: N-(5'-phosphoribosyl)anthranilate isomerase

Products: NA

Alternate protein names: PRAI

Number of amino acids: Translated: 199; Mature: 198

Protein sequence:

>199_residues
MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAELAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQ
VHGQVADDLFENLPCASIQAVQVDGNGHVPNSQADYLLFDAPVAGSGQPFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKNGISRTK

Sequences:

>Translated_199_residues
MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAELAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQ
VHGQVADDLFENLPCASIQAVQVDGNGHVPNSQADYLLFDAPVAGSGQPFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKNGISRTK
>Mature_198_residues
TKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAELAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQV
HGQVADDLFENLPCASIQAVQVDGNGHVPNSQADYLLFDAPVAGSGQPFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQH
FTPYAVDVSSGVETDGQKDHEKIRRFIERVKNGISRTK

Specific function: Bifunctional Enzyme That Catalyzes Two Sequential Steps Of Tryptophan Biosynthetic Pathway. The First Reaction Is Catalyzed By The Isomerase, Coded By The Trpf Domain; The Second Reaction Is Catalyzed By The Synthase, Coded By The Trpc Domain. [C]

COG id: COG0135

COG function: function code E; Phosphoribosylanthranilate isomerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpF family

Homologues:

Organism=Escherichia coli, GI87081863, Length=185, Percent_Identity=34.5945945945946, Blast_Score=69, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320210, Length=212, Percent_Identity=31.6037735849057, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRPF_STRP2 (Q04IY8)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_817050.1
- ProteinModelPortal:   Q04IY8
- SMR:   Q04IY8
- STRING:   Q04IY8
- EnsemblBacteria:   EBSTRT00000019903
- GeneID:   4441409
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_1598
- eggNOG:   COG0135
- GeneTree:   EBGT00050000029364
- HOGENOM:   HBG554803
- OMA:   IQLHGRE
- ProtClustDB:   PRK01222
- HAMAP:   MF_00135_B
- InterPro:   IPR013785
- InterPro:   IPR001240
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF00697 PRAI; SSF51366 RibP_bind_barrel

EC number: =5.3.1.24

Molecular weight: Translated: 21366; Mature: 21235

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAELAKLIPADVKKVGVFV
CCEEEEECCCHHHHHHHHHHCCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHCEEE
SPSRVELLEAIDKVGLDLVQVHGQVADDLFENLPCASIQAVQVDGNGHVPNSQADYLLFD
CCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCEEEEE
APVAGSGQPFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQHFTPYAVDVSSGVETDGQKD
CCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCEEEECCCCCCCCCCHH
HEKIRRFIERVKNGISRTK
HHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAELAKLIPADVKKVGVFV
CEEEEECCCHHHHHHHHHHCCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHCEEE
SPSRVELLEAIDKVGLDLVQVHGQVADDLFENLPCASIQAVQVDGNGHVPNSQADYLLFD
CCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCEEEEE
APVAGSGQPFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQHFTPYAVDVSSGVETDGQKD
CCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCEEEECCCCCCCCCCHH
HEKIRRFIERVKNGISRTK
HHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA