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The map label for this gene is trpA

Identifier: 116515328

GI number: 116515328

Start: 1612073

End: 1612849

Strand: Reverse

Name: trpA

Synonym: SPD_1596

Alternate gene names: 116515328

Gene position: 1612849-1612073 (Counterclockwise)

Preceding gene: 116516622

Following gene: 116517126

Centisome position: 78.82

GC content: 45.3

Gene sequence:

>777_bases
ATGCCTAAGACACTAACAGAAAAATTGAATGCTATAAAAGCAACTGGAAAAGGAATTTTTGTTCCCTATATCATGGCTGG
TGACCACGAGAAAGGTTTGGATGGACTAGGAGAAACAATCCACTTTCTAGAAGATTTGGGTGTCTCTGCAATTGAAGTGG
GTATTCCCTTTTCAGACCCTGTTGCAGATGGACCTGTTATCGAAGAAGCTGGCTTGCGCAGTTTAGCCCACGGGACCTCT
ACCCAGGCTTTAGTTGAAACCTTGAAAACCATTGAAACAGAGATTCCACTGGTCATCATGACCTACTTCAACCCCCTCTT
TCAGTACGGTGTGGAGAACTTTGTCAAAGATCTGGCTGATACAGCTGTGAAAGGCTTGATTATCCCAGACCTGCCTCATG
AACATGCCAACTTTGTAGAACCATTTTTGGCAGATACAGATATCGCCTTGATTCCTTTAGTAAGCTTGACCACAGGAATT
GAGCGCCAGAAAGAGTTGATTGAAGGGGCGGAGGGATTCGTCTATGCCGTTGCCATCAATGGAGTGACAGGGAAATCAGG
CAATTACCGTGCAGATTTGGACAAGCACTTGGCGCAACTTCATCAAGTGGCCGACATCCCAGTCTTGACAGGTTTTGGTG
TATCTAGTCAAGCCGATCTGGAACGCTTCAATGCGGTGTCAGATGGTGTTATCGTCGGTTCGAAAATAGTAAAAGCTCTC
CACCAAGGAGAGCCGATTCAGGACTTTATCAGACAAGCAGTAGCTTACCAAAAATAA

Upstream 100 bases:

>100_bases
GAAAAGTCTATGATTGTCTGCCTATCAGGTCGTGGGGACAAGGATGTGGTTCAAGTCAAAGACCGCTTGGAAGCAGATGC
AGCAAAGAAGGGAGAAGCTC

Downstream 100 bases:

>100_bases
TCCCACAAGCAGCAAGTAAGAGGAAAGGCACGAAAGGAAGTCTTTCCTTTTTCTTTTGCAGGAGAAAGGCTAGTTTTATA
GTGAATTGAATCTGGAATAT

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MPKTLTEKLNAIKATGKGIFVPYIMAGDHEKGLDGLGETIHFLEDLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTS
TQALVETLKTIETEIPLVIMTYFNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
ERQKELIEGAEGFVYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQADLERFNAVSDGVIVGSKIVKAL
HQGEPIQDFIRQAVAYQK

Sequences:

>Translated_258_residues
MPKTLTEKLNAIKATGKGIFVPYIMAGDHEKGLDGLGETIHFLEDLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTS
TQALVETLKTIETEIPLVIMTYFNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
ERQKELIEGAEGFVYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQADLERFNAVSDGVIVGSKIVKAL
HQGEPIQDFIRQAVAYQK
>Mature_257_residues
PKTLTEKLNAIKATGKGIFVPYIMAGDHEKGLDGLGETIHFLEDLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTST
QALVETLKTIETEIPLVIMTYFNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGIE
RQKELIEGAEGFVYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQADLERFNAVSDGVIVGSKIVKALH
QGEPIQDFIRQAVAYQK

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=236, Percent_Identity=29.6610169491525, Blast_Score=99, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6321412, Length=244, Percent_Identity=34.8360655737705, Blast_Score=147, Evalue=2e-36,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_STRP2 (Q04IZ0)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_817048.1
- ProteinModelPortal:   Q04IZ0
- SMR:   Q04IZ0
- STRING:   Q04IZ0
- EnsemblBacteria:   EBSTRT00000019614
- GeneID:   4442671
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_1596
- eggNOG:   COG0159
- GeneTree:   EBGT00050000029204
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- ProtClustDB:   PRK13111
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 27742; Mature: 27611

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 52-52 ACT_SITE 63-63

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKTLTEKLNAIKATGKGIFVPYIMAGDHEKGLDGLGETIHFLEDLGVSAIEVGIPFSDP
CCCHHHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCC
VADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTYFNPLFQYGVENFVKDLAD
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
TAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGIERQKELIEGAEGFVYAVAIN
HHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEE
GVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQADLERFNAVSDGVIVGSKIVKAL
CCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHCCCEEHHHHHHHHH
HQGEPIQDFIRQAVAYQK
HCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
PKTLTEKLNAIKATGKGIFVPYIMAGDHEKGLDGLGETIHFLEDLGVSAIEVGIPFSDP
CCHHHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCC
VADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTYFNPLFQYGVENFVKDLAD
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
TAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGIERQKELIEGAEGFVYAVAIN
HHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEE
GVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQADLERFNAVSDGVIVGSKIVKAL
CCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHCCCEEHHHHHHHHH
HQGEPIQDFIRQAVAYQK
HCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA