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The map label for this gene is ligA

Identifier: 116516470

GI number: 116516470

Start: 1011017

End: 1012975

Strand: Direct

Name: ligA

Synonym: SPD_1001

Alternate gene names: 116516470

Gene position: 1011017-1012975 (Clockwise)

Preceding gene: 116515698

Following gene: 116515362

Centisome position: 49.41

GC content: 44.0

Gene sequence:

>1959_bases
ATGAATAAAAGAATGAATGAGTTAGTCGCTTTGCTCAATCGCTATGCGACTGAGTACTATACCAGCGATAATCCCTCGGT
TTCAGACAGTGAGTATGACCGCCTTTACCGTGAGTTGGTCGAGTTAGAAACTGCTTATCCAGAGCAAGTGCTAGCAGACA
GTCCGACTCATCGTGTTGGTGGCAAGGTTTTAGATGGTTTTGAAAAATACAGTCATCAGTATCCTCTTTATAGTTTGCAG
GATGCTTTTTCACGTGAGGAGCTAGATGCTTTTGATGCGCGTGTTCGTAAGGAAGTGGCTCATCCGACCTATATTTGTGA
GCTGAAAATCGATGGCTTATCTATCTCGCTGACTTATGAAAAGGGGATTTTGGTTGCTGGGGTAACACGTGGAGATGGTT
CAATTGGTGAAAATATCACAGAAAACCTCAAGCGTGTTAAGGACATCCCTTTGACTTTGCCAGAAGAACTAGATATCACA
GTTCGTGGGGAATGTTACATGCCACGCGCTTCCTTTGACCAAGTTAACCAAGCGCGCCAAGAAAATGGAGAGCCTGAATT
TGCTAATCCTCGTAATGCGGCAGCAGGAACTCTGCGTCAGTTGGATACAGCAGTAGTTGCCAAGCGTAATCTTGCAACGT
TTCTCTATCAAGAAGCCAGCCCTTCAACTCGTGATAGCCAAGAAAAGGGTTTGAAGTACCTAGAACAACTAGGTTTTGTG
GTCAATCCTAAGCGAATCTTGGCTGAAAACATAGATGAAATCTGGAATTTTATCCAAGAAGTAGGACAGGAACGGGAAAA
TCTGCCTTACGATATTGATGGAGTGGTAATCAAGGTCAACGACCTAGCAAGTCAAGAAGAACTTGGTTTTACCGTTAAGG
CTCCAAAGTGGGCAGTAGCCTACAAGTTCCCTGCTGAAGAAAAAGAAGCTCAACTCTTATCAGTTGACTGGACAGTTGGC
CGTACCGGTGTTGTAACTCCAACTGCTAATCTAACACCAGTACAACTTGCCGGTACGACTGTTAGCCGTGCGACCCTGCA
CAATGTAGATTATATTGCTGAAAAAGATATCCGAAAAGACGATACGGTCATTGTATATAAGGCTGGTGACATCATCCCTG
CCGTTTTACGTGTGGTAGAGTCCAAACGGGTTTCTGAAGAAAAACTAGATATCCCTACAAACTGTCCAAGTTGTAACTCT
GACTTGTTGCACTTTGAAGATGAAGTGGCCCTACGTTGTATCAATCCGCGTTGCCCTGCTCAAATCATGGAAGGCTTGAT
TCACTTTGCTTCTCGTGATGCTATGAATATTACAGGCCTTGGTCCATCTATTGTTGAGAAGCTTTTTGCTGCTAATTTAG
TCAAGGATGTGGCGGATATTTATCGTTTGCAAGAAGAGGATTTCCTCCTTTTAGAGGGGGTTAAGGAAAAGTCCGCTGCT
AAACTGTATCAGGCTATCCAAGCATCAAAGGAAAATTCTGCCGAGAAGCTCTTATTTGGTTTGGGAATTCGTCATGTCGG
AAGCAAGGCTAGTCAGCTTTTACTTCAATATTTCCATTCAATTGAAAATCTGTATCAGGCAGATTCAGAGGAAGTGGCTA
GTATTGAAAGTCTAGGTGGCGTGATTGCCAAAAGTCTTCAGACTTATTTTGCGGCAGAAGGCTCTGAAATTCTGCTCAGA
GAATTGAAAGAAACTGGGGTCAATCTGGACTATAAAGGACAGACGGTAGTAGCGGATGCGGCCTTGTCAGGTTTGACCGT
GGTATTGACAGGAAAATTGGAACGACTCAAGCGCTCAGAAGCTAAAAGTAAACTCGAAAGTCTGGGTGCCAAAGTGACAG
GTAGTGTTTCTAAAAAGACCGACCTCGTCGTGGTAGGTGCAGACGCTGGAAGTAAACTGCAAAAAGCACAAGAACTTGGT
ATCCAGGTCAGAGATGAGGCATGGCTAGAAAGTTTGTAA

Upstream 100 bases:

>100_bases
TGATATAAGGTTGCTTGAGTTTCAAAAATAATTTCAAACTAAGTGTTTTTTCCGCCCTTCTCGTTTGTGAGGAGGGCTTG
TTTTATGGTAAAATGGTCTT

Downstream 100 bases:

>100_bases
TGGATCGTTTAAAAACAGAGTTTAGAGAATATGACTATGTCTGTTAATTGAGACGAGATTGACAAAAATTTATTAGTGAA
ATAGAAACAAAGTAAAAAGG

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 652; Mature: 652

Protein sequence:

>652_residues
MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELETAYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQ
DAFSREELDAFDARVRKEVAHPTYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
VRGECYMPRASFDQVNQARQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQEASPSTRDSQEKGLKYLEQLGFV
VNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVVIKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVG
RTGVVTPTANLTPVQLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIPTNCPSCNS
DLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFAANLVKDVADIYRLQEEDFLLLEGVKEKSAA
KLYQAIQASKENSAEKLLFGLGIRHVGSKASQLLLQYFHSIENLYQADSEEVASIESLGGVIAKSLQTYFAAEGSEILLR
ELKETGVNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSVSKKTDLVVVGADAGSKLQKAQELG
IQVRDEAWLESL

Sequences:

>Translated_652_residues
MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELETAYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQ
DAFSREELDAFDARVRKEVAHPTYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
VRGECYMPRASFDQVNQARQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQEASPSTRDSQEKGLKYLEQLGFV
VNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVVIKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVG
RTGVVTPTANLTPVQLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIPTNCPSCNS
DLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFAANLVKDVADIYRLQEEDFLLLEGVKEKSAA
KLYQAIQASKENSAEKLLFGLGIRHVGSKASQLLLQYFHSIENLYQADSEEVASIESLGGVIAKSLQTYFAAEGSEILLR
ELKETGVNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSVSKKTDLVVVGADAGSKLQKAQELG
IQVRDEAWLESL
>Mature_652_residues
MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELETAYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQ
DAFSREELDAFDARVRKEVAHPTYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
VRGECYMPRASFDQVNQARQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQEASPSTRDSQEKGLKYLEQLGFV
VNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVVIKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVG
RTGVVTPTANLTPVQLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIPTNCPSCNS
DLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFAANLVKDVADIYRLQEEDFLLLEGVKEKSAA
KLYQAIQASKENSAEKLLFGLGIRHVGSKASQLLLQYFHSIENLYQADSEEVASIESLGGVIAKSLQTYFAAEGSEILLR
ELKETGVNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSVSKKTDLVVVGADAGSKLQKAQELG
IQVRDEAWLESL

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=662, Percent_Identity=42.7492447129909, Blast_Score=521, Evalue=1e-149,
Organism=Escherichia coli, GI87082305, Length=505, Percent_Identity=22.5742574257426, Blast_Score=107, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_STRP2 (Q04KH2)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_816476.1
- ProteinModelPortal:   Q04KH2
- STRING:   Q04KH2
- EnsemblBacteria:   EBSTRT00000020230
- GeneID:   4442940
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_1001
- eggNOG:   COG0272
- GeneTree:   EBGT00050000028051
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   PRK07956
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 72247; Mature: 72247

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 109-109 BINDING 107-107 BINDING 130-130 BINDING 164-164 BINDING 278-278 BINDING 302-302

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELETAYPEQVLADSPTHRVG
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHC
GKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHPTYICELKIDGLSISLTYE
HHHHHHHHHHCCCCCCHHHHHHHCHHHHHHHHHHHHHHHCCCEEEEEEEECCEEEEEEEE
KGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDITVRGECYMPRASFDQVNQARQ
CCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEECCCEECCCCCHHHHHHHHH
ENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQEASPSTRDSQEKGLKYLEQLGFV
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCE
VNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVVIKVNDLASQEELGFTVKAPKWAVA
ECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHCCEEEECCCEEEE
YKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPVQLAGTTVSRATLHNVDYIAEKDIRKD
EECCCCCCCCEEEEEEEECCCCEEECCCCCCCEEEECCCCHHHHHHHCHHHHHHCCCCCC
DTVIVYKAGDIIPAVLRVVESKRVSEEKLDIPTNCPSCNSDLLHFEDEVALRCINPRCPA
CCEEEEECCCHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCEECCCHHHEEECCCCCHH
QIMEGLIHFASRDAMNITGLGPSIVEKLFAANLVKDVADIYRLQEEDFLLLEGVKEKSAA
HHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHH
KLYQAIQASKENSAEKLLFGLGIRHVGSKASQLLLQYFHSIENLYQADSEEVASIESLGG
HHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
VIAKSLQTYFAAEGSEILLRELKETGVNLDYKGQTVVADAALSGLTVVLTGKLERLKRSE
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCEEEEHHHHCCEEEEEECHHHHHHHHH
AKSKLESLGAKVTGSVSKKTDLVVVGADAGSKLQKAQELGIQVRDEAWLESL
HHHHHHHCCCCEECCCCCCCCEEEEECCCCHHHHHHHHCCCEECCHHHHHCC
>Mature Secondary Structure
MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELETAYPEQVLADSPTHRVG
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHC
GKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHPTYICELKIDGLSISLTYE
HHHHHHHHHHCCCCCCHHHHHHHCHHHHHHHHHHHHHHHCCCEEEEEEEECCEEEEEEEE
KGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDITVRGECYMPRASFDQVNQARQ
CCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEECCCEECCCCCHHHHHHHHH
ENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQEASPSTRDSQEKGLKYLEQLGFV
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCE
VNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVVIKVNDLASQEELGFTVKAPKWAVA
ECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHCCEEEECCCEEEE
YKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPVQLAGTTVSRATLHNVDYIAEKDIRKD
EECCCCCCCCEEEEEEEECCCCEEECCCCCCCEEEECCCCHHHHHHHCHHHHHHCCCCCC
DTVIVYKAGDIIPAVLRVVESKRVSEEKLDIPTNCPSCNSDLLHFEDEVALRCINPRCPA
CCEEEEECCCHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCEECCCHHHEEECCCCCHH
QIMEGLIHFASRDAMNITGLGPSIVEKLFAANLVKDVADIYRLQEEDFLLLEGVKEKSAA
HHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHH
KLYQAIQASKENSAEKLLFGLGIRHVGSKASQLLLQYFHSIENLYQADSEEVASIESLGG
HHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
VIAKSLQTYFAAEGSEILLRELKETGVNLDYKGQTVVADAALSGLTVVLTGKLERLKRSE
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCEEEEHHHHCCEEEEEECHHHHHHHHH
AKSKLESLGAKVTGSVSKKTDLVVVGADAGSKLQKAQELGIQVRDEAWLESL
HHHHHHHCCCCEECCCCCCCCEEEEECCCCHHHHHHHHCCCEECCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA