| Definition | Streptococcus pneumoniae D39, complete genome. |
|---|---|
| Accession | NC_008533 |
| Length | 2,046,115 |
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The map label for this gene is pulA [H]
Identifier: 116515362
GI number: 116515362
Start: 1013087
End: 1015366
Strand: Direct
Name: pulA [H]
Synonym: SPD_1002
Alternate gene names: 116515362
Gene position: 1013087-1015366 (Clockwise)
Preceding gene: 116516470
Following gene: 116516523
Centisome position: 49.51
GC content: 41.01
Gene sequence:
>2280_bases ATGTATAACTACCCTATGCGCATTCATTACCATCGTAAGAATGGAGAATATGACACTTGCTCCTTTGTAAAAAGTCAGGA TCAACGAATTGATTTGCTAACCTACAAAGAAGATTATTTTGGAGCTTTATTTAGCTTTGAACACCCAAGTTCACATGTTA TAGAAAGCTTGAATTTTGTGGTTCATACAGGTCAAACTAGTAAAGAATATTCTATCCGTTTTAATCACTATCCCCTATTA ACAGAGGTCTGGATTTTGGAAGGCGATGATAGGATTTATTACTCTGAAAATCCTGCTATTGCCAGTCCTTTCTATAAAAA TCAAAATCCTTTTGCCTTTGATAAGGCCATTAACAGTGCTAGTTTTGATCATCATTGGGGTTACCAAGGAGAATTGGGTT GCCGTGTAGAGGACAATCAGGCTCATTTTTCCCTCTGGTCACCTACAGCGACAGAAGTGCAAGTTGTCGTTTATGAATCA GCTGCTAATGATGCACCCGTTTGGAAGACTTTTGAGATGAAAAGAGGCAATAGCTACTCTTATAATCATAAGGACAATAC AATCGGTGTCTGGAGTTTGGATGTTGAAGAAGATTTGGTAGGTAAGACTTATCAGTATCAAGTCCAATTCCCTCATCACC AAACACTGACACGTGATCCTTATACAATCGCGACCAGCCCTGATGGCAAACGTTCAGCTATTCTGAGCCATGTAGAAAAG CAAGTTGAAAACTTCGAGGTTAAGCACGGTTCGGAGGCTACTTGGCGCTTGGAAAATCCATGTAAGGCAGTTATCTGTGA AATGCACATTCGTGATTTGACTAAATCACCTACATCGGGTGTAGATGAACATCTTCGAGGAACTTTCTTGGGTGCTGCTC AGGCTGGAACAGTTAACCAATACGGCCAGTCAACTGCTTTTGATTACATCAAGAAGCTGGGCTACAATTATGTTCAATTG CAACCAATTGCAGACCGTCATAAAGAATACGATGAGGATGGAAATGTAACCTACAACTGGGGTTATGATCCACAAAACTA TAACGCGCCAGAAACTAGTTTTTCAACTAATCCAGATGATCCAGCTCAGGTCATTCGTGATTTGAAGGTGATGGTTCAAG CTTATCACGATGCGGGTATTGGAGTCATTATGGATGTAGTCTATAACCATACCTTCTCAGTTGTTGATGCACCATTCCAA ACAACAGTCCCTGATTACTATTATCGTATGAATCCAGATGGTACCTTCCAGAATGGAACGGGTGTTGGAAATGAAACAGC CAGTGAACACGAAATGTTTCGCAAGTATATGATTGATTCTCTTCTATACTGGGTGCAGGAATATAATATTGACGGCTTCC GTTTTGACTTGATGGGGATTCATGATGTCAAGACCATGCAGATGATTCGTCAAAGCTTGGATGAAATCGACTCCAACATT ATCCTCTATGGAGAAGGATGGGATATGGGAACAGGTCTTGCCCCTTATGATAAGGCCAAGAAGGACAATGCCTACCAGAT GCCAAATATTGGTTTCTTTAATGACAATCAGCGCGATGCTGTCAAAGGGGGAGAAGTTTATGGTGCTATCAAGTCAGGTT TTGTCAGTGGTGCTGCGACAGAGCCAATTCTAGCTAAAGCAATCCTAGGAAGTCGTGAATTAGGAAGCTATACACATCCA AATCAGGTGCTTAACTATGTAGAAGCCCATGACAATTACAATCTTCACGATTTATTGGCAACCCTTCATCCAGACCAAAG TTCAGAGCAAATCATGCGCAAGGTCGAAACTGCCACAGCCATGAATCTGCTCATGCAGGGGATGGCCTTTATGGAAATCG GTCAAGAATTTGGTCGTACCAAACTGGTTGCGACTGGTGAAAATGGTGAGTTGACCCATGATGATAGAGAGCGTGCGATG AATAGCTATAATGCTCCTGACAGTGTGAACCAAGTGAACTGGAACTTGATTAATGAGCGTCAAGACAGTATTGAGTTTAT CCGTCAAGTCATCCGATTGAAGACAAAAACTGGTGCCTTTTCTTACTCTAGCTATGATGAAATTTACCATCATGTCTTTG TGCATTCTGCGATTGAACATAGCGGCTGCCTTATCTATGAAGTTCACGGCAAAGAACACCTCTTGGTAGTTGTGAATGCT AAATCAGAACCCTATCAATTTGAAAATGCAGGAAATTTAGCTATGTTGGTAACCAACAGTCGCTCAAAAGAAGATAATGT TTTAAATGATATTAGTCTAGCTGTCTTGAGTGTTTTATAA
Upstream 100 bases:
>100_bases AAAACAGAGTTTAGAGAATATGACTATGTCTGTTAATTGAGACGAGATTGACAAAAATTTATTAGTGAAATAGAAACAAA GTAAAAAGGAAAAATAAAAA
Downstream 100 bases:
>100_bases ATACCATCTATCTCGCTATTTTTACACAGACAAATTCCCAGTTATGAGTATTGATAAAAAATCCATTCATTGAAATTGAA AGAATAGAAAGAACCTGGCT
Product: pullulanase, type I
Products: NA
Alternate protein names: Alpha-dextrin endo-1,6-alpha-glucosidase; Pullulan 6-glucanohydrolase [H]
Number of amino acids: Translated: 759; Mature: 759
Protein sequence:
>759_residues MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALFSFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLL TEVWILEGDDRIYYSENPAIASPFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKTYQYQVQFPHHQTLTRDPYTIATSPDGKRSAILSHVEK QVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKSPTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQL QPIADRHKEYDEDGNVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTFSVVDAPFQ TTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNIDGFRFDLMGIHDVKTMQMIRQSLDEIDSNI ILYGEGWDMGTGLAPYDKAKKDNAYQMPNIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHP NQVLNYVEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVATGENGELTHDDRERAM NSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSYSSYDEIYHHVFVHSAIEHSGCLIYEVHGKEHLLVVVNA KSEPYQFENAGNLAMLVTNSRSKEDNVLNDISLAVLSVL
Sequences:
>Translated_759_residues MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALFSFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLL TEVWILEGDDRIYYSENPAIASPFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKTYQYQVQFPHHQTLTRDPYTIATSPDGKRSAILSHVEK QVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKSPTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQL QPIADRHKEYDEDGNVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTFSVVDAPFQ TTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNIDGFRFDLMGIHDVKTMQMIRQSLDEIDSNI ILYGEGWDMGTGLAPYDKAKKDNAYQMPNIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHP NQVLNYVEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVATGENGELTHDDRERAM NSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSYSSYDEIYHHVFVHSAIEHSGCLIYEVHGKEHLLVVVNA KSEPYQFENAGNLAMLVTNSRSKEDNVLNDISLAVLSVL >Mature_759_residues MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALFSFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLL TEVWILEGDDRIYYSENPAIASPFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKTYQYQVQFPHHQTLTRDPYTIATSPDGKRSAILSHVEK QVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKSPTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQL QPIADRHKEYDEDGNVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTFSVVDAPFQ TTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNIDGFRFDLMGIHDVKTMQMIRQSLDEIDSNI ILYGEGWDMGTGLAPYDKAKKDNAYQMPNIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHP NQVLNYVEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVATGENGELTHDDRERAM NSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSYSSYDEIYHHVFVHSAIEHSGCLIYEVHGKEHLLVVVNA KSEPYQFENAGNLAMLVTNSRSKEDNVLNDISLAVLSVL
Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]
COG id: COG1523
COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the glycosyl hydrolase 13 family [H]
Homologues:
Organism=Escherichia coli, GI2367229, Length=499, Percent_Identity=24.0480961923848, Blast_Score=105, Evalue=1e-23,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR006047 - InterPro: IPR004193 - InterPro: IPR017853 - InterPro: IPR013781 - InterPro: IPR013783 - InterPro: IPR011840 [H]
Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]
EC number: =3.2.1.41 [H]
Molecular weight: Translated: 86518; Mature: 86518
Theoretical pI: Translated: 5.02; Mature: 5.02
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.7 %Cys (Translated Protein) 2.8 %Met (Translated Protein) 3.4 %Cys+Met (Translated Protein) 0.7 %Cys (Mature Protein) 2.8 %Met (Mature Protein) 3.4 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALFSFEHPSSHVIESLNFV CCCCCEEEEEECCCCCCCHHHHHCCCCCEEEEEEECHHHHHHEEECCCCHHHHHHHCEEE VHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIASPFYKNQNPFAFDKAINSA EECCCCCCEEEEEECCCCCEEEEEEEECCCEEEECCCCCCCCCCCCCCCCCHHHHHHCCC SFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYESAANDAPVWKTFEMKRGNSYS CCCCCCCCCCCCCCEEECCCCEEEEECCCCCEEEEEEEECCCCCCCCEEEEEECCCCCCC YNHKDNTIGVWSLDVEEDLVGKTYQYQVQFPHHQTLTRDPYTIATSPDGKRSAILSHVEK CCCCCCEEEEEEECCCHHHCCCEEEEEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHH QVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKSPTSGVDEHLRGTFLGAAQAGTVNQ HHHHCEECCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHEEECCCCCCCHHH YGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDGNVTYNWGYDPQNYNAPETSFSTNPDD CCCHHHHHHHHHCCCCEEEEEEHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCC PAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTFSVVDAPFQTTVPDYYYRMNPDGTFQNGT HHHHHHHHHHHHHHHHCCCHHHHHHHHHCCEEEEECCCCCCCCCCEEEEECCCCCCCCCC GVGNETASEHEMFRKYMIDSLLYWVQEYNIDGFRFDLMGIHDVKTMQMIRQSLDEIDSNI CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCE ILYGEGWDMGTGLAPYDKAKKDNAYQMPNIGFFNDNQRDAVKGGEVYGAIKSGFVSGAAT EEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCEEEHHHHCCCCCCCC EPILAKAILGSRELGSYTHPNQVLNYVEAHDNYNLHDLLATLHPDQSSEQIMRKVETATA CHHHHHHHHCCHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHH MNLLMQGMAFMEIGQEFGRTKLVATGENGELTHDDRERAMNSYNAPDSVNQVNWNLINER HHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCCEEECCCC QDSIEFIRQVIRLKTKTGAFSYSSYDEIYHHVFVHSAIEHSGCLIYEVHGKEHLLVVVNA HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEEEEC KSEPYQFENAGNLAMLVTNSRSKEDNVLNDISLAVLSVL CCCCEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHCC >Mature Secondary Structure MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALFSFEHPSSHVIESLNFV CCCCCEEEEEECCCCCCCHHHHHCCCCCEEEEEEECHHHHHHEEECCCCHHHHHHHCEEE VHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIASPFYKNQNPFAFDKAINSA EECCCCCCEEEEEECCCCCEEEEEEEECCCEEEECCCCCCCCCCCCCCCCCHHHHHHCCC SFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYESAANDAPVWKTFEMKRGNSYS CCCCCCCCCCCCCCEEECCCCEEEEECCCCCEEEEEEEECCCCCCCCEEEEEECCCCCCC YNHKDNTIGVWSLDVEEDLVGKTYQYQVQFPHHQTLTRDPYTIATSPDGKRSAILSHVEK CCCCCCEEEEEEECCCHHHCCCEEEEEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHH QVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKSPTSGVDEHLRGTFLGAAQAGTVNQ HHHHCEECCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHEEECCCCCCCHHH YGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDGNVTYNWGYDPQNYNAPETSFSTNPDD CCCHHHHHHHHHCCCCEEEEEEHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCC PAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTFSVVDAPFQTTVPDYYYRMNPDGTFQNGT HHHHHHHHHHHHHHHHCCCHHHHHHHHHCCEEEEECCCCCCCCCCEEEEECCCCCCCCCC GVGNETASEHEMFRKYMIDSLLYWVQEYNIDGFRFDLMGIHDVKTMQMIRQSLDEIDSNI CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCE ILYGEGWDMGTGLAPYDKAKKDNAYQMPNIGFFNDNQRDAVKGGEVYGAIKSGFVSGAAT EEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCEEEHHHHCCCCCCCC EPILAKAILGSRELGSYTHPNQVLNYVEAHDNYNLHDLLATLHPDQSSEQIMRKVETATA CHHHHHHHHCCHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHH MNLLMQGMAFMEIGQEFGRTKLVATGENGELTHDDRERAMNSYNAPDSVNQVNWNLINER HHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCCEEECCCC QDSIEFIRQVIRLKTKTGAFSYSSYDEIYHHVFVHSAIEHSGCLIYEVHGKEHLLVVVNA HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEEEEC KSEPYQFENAGNLAMLVTNSRSKEDNVLNDISLAVLSVL CCCCEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9387221; 9384377 [H]