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The map label for this gene is folE

Identifier: 116515945

GI number: 116515945

Start: 270321

End: 270875

Strand: Direct

Name: folE

Synonym: SPD_0271

Alternate gene names: 116515945

Gene position: 270321-270875 (Clockwise)

Preceding gene: 116516745

Following gene: 116517074

Centisome position: 13.21

GC content: 43.06

Gene sequence:

>555_bases
ATGGATACACAAAAGATTGAAGCAGCTGTAAAAATGATTATCGAGGCTGTAGGAGAGGACGCTAATCGCGAGGGTTTGCA
GGAAACACCTGCTCGTGTAGCCCGTATGTATCAAGAGATTTTTTCAGGTCTTGGTCAAACAGCAGAGGAACATTTGTCAA
AATCCTTTGAAATTATTGACGATAATATGGTGGTAGAAAAGGATATCTTTTTCCATACCATGTGTGAACACCACTTCTTG
CCATTTTATGGTAGAGCGCACATTGCCTACATTCCAGATGGTCGTGTGGCAGGCTTGTCTAAGCTAGCCCGTACGGTTGA
AGTTTATTCGAAAAAACCACAAATTCAAGAACGTTTGAATATCGAAGTGGCCGATGCCTTGATGGACTATCTAGGTGCTA
AAGGAGCCTTTGTTATCATTGAGGCGGAACATATGTGTATGAGTATGCGTGGTGTTAGAAAACCAGGCACTGCAACCTTG
ACGACAGTAGCTCGTGGTCTATTTGAAACAGATAAGGACCTCCGCGACCAAGCTTATCGTTTAATGGGGCTATAA

Upstream 100 bases:

>100_bases
ACAGATAAAAAAGAAGAGAAACAAACAGTTAGGATTGTCACAGGTTCCTTGTATTTCTTGAGCCAAGTGAGGGTCTATCT
GATGGAGAGGAAGAACGAGA

Downstream 100 bases:

>100_bases
AAAGAATCCGTTTCAAGCGGATTTTTCTAGAAAGGAATCATTATGGATCAACTGCAGATTAAGGATTTGGAAATGTTTGC
CTATCATGGTCTTTTTCCTA

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MDTQKIEAAVKMIIEAVGEDANREGLQETPARVARMYQEIFSGLGQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFL
PFYGRAHIAYIPDGRVAGLSKLARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVIIEAEHMCMSMRGVRKPGTATL
TTVARGLFETDKDLRDQAYRLMGL

Sequences:

>Translated_184_residues
MDTQKIEAAVKMIIEAVGEDANREGLQETPARVARMYQEIFSGLGQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFL
PFYGRAHIAYIPDGRVAGLSKLARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVIIEAEHMCMSMRGVRKPGTATL
TTVARGLFETDKDLRDQAYRLMGL
>Mature_184_residues
MDTQKIEAAVKMIIEAVGEDANREGLQETPARVARMYQEIFSGLGQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFL
PFYGRAHIAYIPDGRVAGLSKLARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVIIEAEHMCMSMRGVRKPGTATL
TTVARGLFETDKDLRDQAYRLMGL

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family

Homologues:

Organism=Homo sapiens, GI66932968, Length=183, Percent_Identity=40.9836065573771, Blast_Score=149, Evalue=1e-36,
Organism=Homo sapiens, GI4503949, Length=183, Percent_Identity=40.9836065573771, Blast_Score=149, Evalue=1e-36,
Organism=Homo sapiens, GI66932970, Length=143, Percent_Identity=41.2587412587413, Blast_Score=117, Evalue=5e-27,
Organism=Homo sapiens, GI66932972, Length=143, Percent_Identity=41.2587412587413, Blast_Score=117, Evalue=8e-27,
Organism=Escherichia coli, GI1788476, Length=170, Percent_Identity=38.8235294117647, Blast_Score=114, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17560486, Length=176, Percent_Identity=43.1818181818182, Blast_Score=153, Evalue=6e-38,
Organism=Saccharomyces cerevisiae, GI6321706, Length=182, Percent_Identity=42.8571428571429, Blast_Score=154, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24656782, Length=174, Percent_Identity=39.6551724137931, Blast_Score=132, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24656796, Length=174, Percent_Identity=39.6551724137931, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24656791, Length=174, Percent_Identity=39.6551724137931, Blast_Score=132, Evalue=2e-31,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): GCH1_STRP2 (Q04MF9)

Other databases:

- EMBL:   CP000410
- RefSeq:   YP_815789.1
- ProteinModelPortal:   Q04MF9
- SMR:   Q04MF9
- STRING:   Q04MF9
- EnsemblBacteria:   EBSTRT00000020396
- GeneID:   4441347
- GenomeReviews:   CP000410_GR
- KEGG:   spd:SPD_0271
- eggNOG:   COG0302
- GeneTree:   EBGT00050000028325
- HOGENOM:   HBG370191
- OMA:   ARFMSMI
- ProtClustDB:   PRK09347
- GO:   GO:0005737
- HAMAP:   MF_00223
- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234
- TIGRFAMs:   TIGR00063

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =3.5.4.16

Molecular weight: Translated: 20728; Mature: 20728

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTQKIEAAVKMIIEAVGEDANREGLQETPARVARMYQEIFSGLGQTAEEHLSKSFEIID
CCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHC
DNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKLARTVEVYSKKPQIQERLN
CCEEEHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHC
IEVADALMDYLGAKGAFVIIEAEHMCMSMRGVRKPGTATLTTVARGLFETDKDLRDQAYR
HHHHHHHHHHHCCCCCEEEEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHH
LMGL
HHCC
>Mature Secondary Structure
MDTQKIEAAVKMIIEAVGEDANREGLQETPARVARMYQEIFSGLGQTAEEHLSKSFEIID
CCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHC
DNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKLARTVEVYSKKPQIQERLN
CCEEEHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHC
IEVADALMDYLGAKGAFVIIEAEHMCMSMRGVRKPGTATLTTVARGLFETDKDLRDQAYR
HHHHHHHHHHHCCCCCEEEEEHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHH
LMGL
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA