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The map label for this gene is folC [H]

Identifier: 116516745

GI number: 116516745

Start: 269018

End: 270340

Strand: Direct

Name: folC [H]

Synonym: SPD_0270

Alternate gene names: 116516745

Gene position: 269018-270340 (Clockwise)

Preceding gene: 116516653

Following gene: 116515945

Centisome position: 13.15

GC content: 46.11

Gene sequence:

>1323_bases
ATGAAAGAAATTGAAAACAATCAATGGATTGCTAACTACCGGACGGATCAACCGCATTTTGGCTTGGAACGAATGGTGGA
ACTGTTAGCTTTGCGTGGCAATCCCCATCTCAAACTCAAGGTCCTCCATATCGGAGGAACTAACGGCAAGGGCTCGACTA
TTGCTTTTTTGAAAAAGATGCTAGAAAAGCTAGGGCTGAGAGTTGGCGTGTTTAGCTCGCCCTATCTCATTCATTACACA
GACCAGATTAGCATCAATGGGGAATCGATCTCAGAAGCGAGGCTAGAAGCTCTCATGGCAGACTATCAGTCTTTGCTGGA
GGGAGAAGCGGTCGCCAATTTACAGGGCACAACCGAGTTTGAGATTATCACAGCCCTGGCCTATGACTACTTTGCCTCAG
AGCAAGTAGATGTGGCCATCATGGAAGTTGGCATGGGTGGACTTTTTGATAGTACCAATGTCTGTCAGCCCATTTTGACA
GGAATTACAACTATTGGCTTGGATCATGTGGCCTTACTTGGTGACACCTTGGAGGCCATAGCAGAGCAGAAGGCAGGTAT
TATCAAACAAGGGATGCCCTTGGTAACAGGGCGTATTGCTCCAGAAGCCTTGGCTGTGATTGACCGCATTGCGGAAGGGA
AAGATGCGCCGAGACTTGCCTACGGGACAGATTATCAGGTTCGTCATCAAGAAAGTGTGGTGACAGGGGAAGTCTTTGAC
TATACAAGTGCTGTCAGACAAGGTCGCTTCCAGACTAGCCTGCTTGGTTTGTACCAAATAGAGAATGCTGGGATGGCCAT
AGCTTTACTTGATACTTTTTGTCAAGAAGATGGTCGAGAGCTAGCAAGCAATGACTTTCTTGGTCAAGCCTTGGAAGAAA
CAAGTTGGCCAGGGCGTTTGGAAATCGTGTCAAGAGATCCCTTGATGATTTTGGATGGAGCCCATAATCCCCATGCTATC
AAGGCCTTGTTGGTAACCTTGCAAGAACGTTTTGCGGATTATCATAAGGAAATCCTCTTCACTTGTATCAAAACCAAAGC
CTTGGAGGATATGTTGGACTTGCTGGGAGCCATGCCAGATACCGAGCTTACTCTAACACATTTTGCGGATAGTCGGGCGA
CGGATGAAAGCGTGCTGAAAGAGGCAGCTAAGTCTAGAAATCTCAGCTACCAAGATTGGCATGATTTTCTAGAGCAGAAT
GTGACAGATAAAAAAGAAGAGAAACAAACAGTTAGGATTGTCACAGGTTCCTTGTATTTCTTGAGCCAAGTGAGGGTCTA
TCTGATGGAGAGGAAGAACGAGAATGGATACACAAAAGATTGA

Upstream 100 bases:

>100_bases
GTGCATGACGTAGCTAGTCACAGGATGGCAGTTGAAATTGCCTCTGCCATTCGTCTGGCTGATGAAGCGGAAAATTTAGA
TTTAAAACAATATAAATAAG

Downstream 100 bases:

>100_bases
AGCAGCTGTAAAAATGATTATCGAGGCTGTAGGAGAGGACGCTAATCGCGAGGGTTTGCAGGAAACACCTGCTCGTGTAG
CCCGTATGTATCAAGAGATT

Product: folylpolyglutamate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHIGGTNGKGSTIAFLKKMLEKLGLRVGVFSSPYLIHYT
DQISINGESISEARLEALMADYQSLLEGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLFDSTNVCQPILT
GITTIGLDHVALLGDTLEAIAEQKAGIIKQGMPLVTGRIAPEALAVIDRIAEGKDAPRLAYGTDYQVRHQESVVTGEVFD
YTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQEDGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAI
KALLVTLQERFADYHKEILFTCIKTKALEDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQDWHDFLEQN
VTDKKEEKQTVRIVTGSLYFLSQVRVYLMERKNENGYTKD

Sequences:

>Translated_440_residues
MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHIGGTNGKGSTIAFLKKMLEKLGLRVGVFSSPYLIHYT
DQISINGESISEARLEALMADYQSLLEGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLFDSTNVCQPILT
GITTIGLDHVALLGDTLEAIAEQKAGIIKQGMPLVTGRIAPEALAVIDRIAEGKDAPRLAYGTDYQVRHQESVVTGEVFD
YTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQEDGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAI
KALLVTLQERFADYHKEILFTCIKTKALEDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQDWHDFLEQN
VTDKKEEKQTVRIVTGSLYFLSQVRVYLMERKNENGYTKD
>Mature_440_residues
MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHIGGTNGKGSTIAFLKKMLEKLGLRVGVFSSPYLIHYT
DQISINGESISEARLEALMADYQSLLEGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLFDSTNVCQPILT
GITTIGLDHVALLGDTLEAIAEQKAGIIKQGMPLVTGRIAPEALAVIDRIAEGKDAPRLAYGTDYQVRHQESVVTGEVFD
YTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQEDGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAI
KALLVTLQERFADYHKEILFTCIKTKALEDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQDWHDFLEQN
VTDKKEEKQTVRIVTGSLYFLSQVRVYLMERKNENGYTKD

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=312, Percent_Identity=33.6538461538462, Blast_Score=155, Evalue=9e-38,
Organism=Homo sapiens, GI66932990, Length=312, Percent_Identity=33.6538461538462, Blast_Score=154, Evalue=1e-37,
Organism=Escherichia coli, GI1788654, Length=428, Percent_Identity=28.2710280373832, Blast_Score=132, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17553150, Length=413, Percent_Identity=28.3292978208232, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17553148, Length=413, Percent_Identity=28.3292978208232, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI71984923, Length=413, Percent_Identity=28.3292978208232, Blast_Score=166, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6323760, Length=427, Percent_Identity=30.6791569086651, Blast_Score=182, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6324815, Length=329, Percent_Identity=29.1793313069909, Blast_Score=127, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6322718, Length=295, Percent_Identity=28.4745762711864, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24641571, Length=337, Percent_Identity=30.2670623145401, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24581568, Length=211, Percent_Identity=33.175355450237, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 48998; Mature: 48998

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHIGGTNGKGSTIAFLKKM
CCCCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEEECCCCCCHHHHHHHHH
LEKLGLRVGVFSSPYLIHYTDQISINGESISEARLEALMADYQSLLEGEAVANLQGTTEF
HHHCCCEEEECCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCHH
EIITALAYDYFASEQVDVAIMEVGMGGLFDSTNVCQPILTGITTIGLDHVALLGDTLEAI
HHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEQKAGIIKQGMPLVTGRIAPEALAVIDRIAEGKDAPRLAYGTDYQVRHQESVVTGEVFD
HHHHCCHHHCCCCCEECCCCHHHHHHHHHHHCCCCCCEEECCCCCEEECCCCHHCCHHHH
YTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQEDGRELASNDFLGQALEETSWPGRL
HHHHHHCCCHHHHHHHHEEECCCCEEHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEE
EIVSRDPLMILDGAHNPHAIKALLVTLQERFADYHKEILFTCIKTKALEDMLDLLGAMPD
EEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TELTLTHFADSRATDESVLKEAAKSRNLSYQDWHDFLEQNVTDKKEEKQTVRIVTGSLYF
CCEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHCCEEEEEECHHHH
LSQVRVYLMERKNENGYTKD
HHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHIGGTNGKGSTIAFLKKM
CCCCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEEECCCCCCHHHHHHHHH
LEKLGLRVGVFSSPYLIHYTDQISINGESISEARLEALMADYQSLLEGEAVANLQGTTEF
HHHCCCEEEECCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCHH
EIITALAYDYFASEQVDVAIMEVGMGGLFDSTNVCQPILTGITTIGLDHVALLGDTLEAI
HHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEQKAGIIKQGMPLVTGRIAPEALAVIDRIAEGKDAPRLAYGTDYQVRHQESVVTGEVFD
HHHHCCHHHCCCCCEECCCCHHHHHHHHHHHCCCCCCEEECCCCCEEECCCCHHCCHHHH
YTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQEDGRELASNDFLGQALEETSWPGRL
HHHHHHCCCHHHHHHHHEEECCCCEEHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEE
EIVSRDPLMILDGAHNPHAIKALLVTLQERFADYHKEILFTCIKTKALEDMLDLLGAMPD
EEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TELTLTHFADSRATDESVLKEAAKSRNLSYQDWHDFLEQNVTDKKEEKQTVRIVTGSLYF
CCEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHCCEEEEEECHHHH
LSQVRVYLMERKNENGYTKD
HHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]