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The map label for this gene is tgt [H]

Identifier: 116494315

GI number: 116494315

Start: 766221

End: 767363

Strand: Direct

Name: tgt [H]

Synonym: LSEI_0771

Alternate gene names: 116494315

Gene position: 766221-767363 (Clockwise)

Preceding gene: 116494314

Following gene: 116494316

Centisome position: 26.46

GC content: 47.94

Gene sequence:

>1143_bases
ATGGAACCAGCAGTTAAGTATCGTCTGATTCATCAGGATAAGCATAGCGGTGCGCGGTTGGGTGAGTTGATCACGCCGCA
CGGCACATTTCCCACCCCAATGTTTATGCCTGTTGGCACACAGGCGAGCGTGAAAACAATGGCGCCTGAAGAATTGGATA
CGATGGGGGCCACTGTTATCTTGGCGAACACGTATCATCTGTGGTTACGGCCGGGGCCAGAACTGATTGAAGAAGCGGGC
GGCTTGCACAATTTTATGAACTGGCACAAAGGTATCCTGACTGACTCAGGTGGCTTTCAAGTCTTTTCGCTAGCCGACAC
TAGACATATTACTGAAGAAGGTGTCACTTTCAAAAATCATTTGAATGGTGCCAAGATGTTCCTGTCCCCAGAAAAGGCGA
TCGCAATTGAAAACGCGTTGGGACCTGACATCATGATGAGTTTGGATGAATGTCCGCCGTTTTTTGAAAGCTATGATTAT
GTTAAAAAGAGTGTTGCTCGAACCAGTCGTTGGGCTGAACGTGGCTTGCAGGCTCACAAGAATCCTGATTGGCAGGCGCT
TTTTGGTATTGTGCAAGGTGCCGGATTCAAAGACTTGCGCGAGCAAAGTGCTAAGGATCTCGTTAGCATGGATTTTCCTG
GCTATTCGATTGGTGGCTTGTCTGTTGGTGAATCAAAAGATGAGATGAACCATGTCCTTGATTTCACTACCCCGCTGCTG
CCGGAAAACAAACCGCGCTATTTGATGGGTGTCGGCTCTGCGGATGCGCTGATTGATGGCGCCTTGCGTGGCATTGATAT
GTTTGACTGCGTCTTACCAACTCGGATTGCCCGAAATGGTACTTGCATGACCAGCCGCGGCCGGCTCGTCATTAAAAATG
CGAAATATGCGCATGACTTTGGACCGCTGGATCCTAATTGCGATTGCTACACGTGTCGTAACTATAGCCGCGCCTATATT
CGCCATCTGATCCATGTTGGTGAAGCTTTCGGTATTCGGTTGACGACCTACCATAATCTCTACTTCTTACTGCACTTGAT
GCAACAAGTGCGTGAAGCGATTGCCAATGATGATTTGCTGGCTTTCCGTGAAGATTTCTTTGAAGCATACGGATACAACA
AGGAAAAAGCAAAGAATTTTTAG

Upstream 100 bases:

>100_bases
ATGCACTCACTTTTAAATAACAAGTAACTCTGTTAAACTGACGTCACGAGAAAATCAAGCGGCGGTGCTGCGAGGGCATT
GCCATGAATGAGGAGAATGT

Downstream 100 bases:

>100_bases
ATTATTTGGAGTAGACCGCCTAATTCTGTTACACTGTTTGTTGACAAAATAAAAATAAGAGGTGCCTAAATTGAATGGTA
ATATGACAATGATCATTATG

Product: queuine/archaeosine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MEPAVKYRLIHQDKHSGARLGELITPHGTFPTPMFMPVGTQASVKTMAPEELDTMGATVILANTYHLWLRPGPELIEEAG
GLHNFMNWHKGILTDSGGFQVFSLADTRHITEEGVTFKNHLNGAKMFLSPEKAIAIENALGPDIMMSLDECPPFFESYDY
VKKSVARTSRWAERGLQAHKNPDWQALFGIVQGAGFKDLREQSAKDLVSMDFPGYSIGGLSVGESKDEMNHVLDFTTPLL
PENKPRYLMGVGSADALIDGALRGIDMFDCVLPTRIARNGTCMTSRGRLVIKNAKYAHDFGPLDPNCDCYTCRNYSRAYI
RHLIHVGEAFGIRLTTYHNLYFLLHLMQQVREAIANDDLLAFREDFFEAYGYNKEKAKNF

Sequences:

>Translated_380_residues
MEPAVKYRLIHQDKHSGARLGELITPHGTFPTPMFMPVGTQASVKTMAPEELDTMGATVILANTYHLWLRPGPELIEEAG
GLHNFMNWHKGILTDSGGFQVFSLADTRHITEEGVTFKNHLNGAKMFLSPEKAIAIENALGPDIMMSLDECPPFFESYDY
VKKSVARTSRWAERGLQAHKNPDWQALFGIVQGAGFKDLREQSAKDLVSMDFPGYSIGGLSVGESKDEMNHVLDFTTPLL
PENKPRYLMGVGSADALIDGALRGIDMFDCVLPTRIARNGTCMTSRGRLVIKNAKYAHDFGPLDPNCDCYTCRNYSRAYI
RHLIHVGEAFGIRLTTYHNLYFLLHLMQQVREAIANDDLLAFREDFFEAYGYNKEKAKNF
>Mature_380_residues
MEPAVKYRLIHQDKHSGARLGELITPHGTFPTPMFMPVGTQASVKTMAPEELDTMGATVILANTYHLWLRPGPELIEEAG
GLHNFMNWHKGILTDSGGFQVFSLADTRHITEEGVTFKNHLNGAKMFLSPEKAIAIENALGPDIMMSLDECPPFFESYDY
VKKSVARTSRWAERGLQAHKNPDWQALFGIVQGAGFKDLREQSAKDLVSMDFPGYSIGGLSVGESKDEMNHVLDFTTPLL
PENKPRYLMGVGSADALIDGALRGIDMFDCVLPTRIARNGTCMTSRGRLVIKNAKYAHDFGPLDPNCDCYTCRNYSRAYI
RHLIHVGEAFGIRLTTYHNLYFLLHLMQQVREAIANDDLLAFREDFFEAYGYNKEKAKNF

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=365, Percent_Identity=46.5753424657534, Blast_Score=346, Evalue=2e-95,
Organism=Escherichia coli, GI1786607, Length=355, Percent_Identity=52.3943661971831, Blast_Score=388, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17544680, Length=354, Percent_Identity=46.045197740113, Blast_Score=303, Evalue=1e-82,
Organism=Caenorhabditis elegans, GI25146502, Length=213, Percent_Identity=27.6995305164319, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19920510, Length=365, Percent_Identity=43.5616438356164, Blast_Score=320, Evalue=1e-87,
Organism=Drosophila melanogaster, GI21355311, Length=258, Percent_Identity=27.1317829457364, Blast_Score=86, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 42648; Mature: 42648

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPAVKYRLIHQDKHSGARLGELITPHGTFPTPMFMPVGTQASVKTMAPEELDTMGATVI
CCCCCEEEEEECCCCCCCHHHHHCCCCCCCCCCEEEECCCCCCHHHCCHHHHHHCCCEEE
LANTYHLWLRPGPELIEEAGGLHNFMNWHKGILTDSGGFQVFSLADTRHITEEGVTFKNH
EEEEEEEEECCCHHHHHHCCCHHHHHHHHCCCEECCCCEEEEEECCCHHHHHCCCCHHHC
LNGAKMFLSPEKAIAIENALGPDIMMSLDECPPFFESYDYVKKSVARTSRWAERGLQAHK
CCCCEEEECCCHHEEECCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
NPDWQALFGIVQGAGFKDLREQSAKDLVSMDFPGYSIGGLSVGESKDEMNHVLDFTTPLL
CCCHHHHHHHHHCCCHHHHHHHHHHHHEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
PENKPRYLMGVGSADALIDGALRGIDMFDCVLPTRIARNGTCMTSRGRLVIKNAKYAHDF
CCCCCCEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEECCCEEEEECCCHHCCC
GPLDPNCDCYTCRNYSRAYIRHLIHVGEAFGIRLTTYHNLYFLLHLMQQVREAIANDDLL
CCCCCCCCEEECCCHHHHHHHHHHHHCHHHEEEEEHHHHHHHHHHHHHHHHHHHCCCCHH
AFREDFFEAYGYNKEKAKNF
HHHHHHHHHHCCCHHHCCCC
>Mature Secondary Structure
MEPAVKYRLIHQDKHSGARLGELITPHGTFPTPMFMPVGTQASVKTMAPEELDTMGATVI
CCCCCEEEEEECCCCCCCHHHHHCCCCCCCCCCEEEECCCCCCHHHCCHHHHHHCCCEEE
LANTYHLWLRPGPELIEEAGGLHNFMNWHKGILTDSGGFQVFSLADTRHITEEGVTFKNH
EEEEEEEEECCCHHHHHHCCCHHHHHHHHCCCEECCCCEEEEEECCCHHHHHCCCCHHHC
LNGAKMFLSPEKAIAIENALGPDIMMSLDECPPFFESYDYVKKSVARTSRWAERGLQAHK
CCCCEEEECCCHHEEECCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
NPDWQALFGIVQGAGFKDLREQSAKDLVSMDFPGYSIGGLSVGESKDEMNHVLDFTTPLL
CCCHHHHHHHHHCCCHHHHHHHHHHHHEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
PENKPRYLMGVGSADALIDGALRGIDMFDCVLPTRIARNGTCMTSRGRLVIKNAKYAHDF
CCCCCCEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEECCCEEEEECCCHHCCC
GPLDPNCDCYTCRNYSRAYIRHLIHVGEAFGIRLTTYHNLYFLLHLMQQVREAIANDDLL
CCCCCCCCEEECCCHHHHHHHHHHHHCHHHEEEEEHHHHHHHHHHHHHHHHHHHCCCCHH
AFREDFFEAYGYNKEKAKNF
HHHHHHHHHHCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12566566 [H]