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The map label for this gene is queA [H]

Identifier: 116494314

GI number: 116494314

Start: 764630

End: 765700

Strand: Direct

Name: queA [H]

Synonym: LSEI_0770

Alternate gene names: 116494314

Gene position: 764630-765700 (Clockwise)

Preceding gene: 116494313

Following gene: 116494315

Centisome position: 26.41

GC content: 45.56

Gene sequence:

>1071_bases
GTGCAAGAGAACAAACGTTATCACAAAGTAGAAGACAAACATTATCAGTTATCGGATTTCGATTATGACTTGCCGCATGA
ATTGATTGCGCAGACACCTTTGAAGGAACGTGATGCCAGTCGGCTGTTGGTATTAAATTCTGAAACTGGTGAGATGCAAG
ATAAGCATTTTTACGACATTCTTGATTATTTCAAGCCAGGTGATGCGTTGGTCATGAATGATACGCGGGTGATGCCAGCG
CGATTGTATGGGGTTAAGCCAGAGACTGGTGCCCATATGGAAGTGTTATTGCTTAACAACACGTCTGGTGATGATTGGGA
AACCTTGATGAAGCCTGCGCGCAAGGCTCCGGTTGGCACGGTGATTGATTTTGGCGATGGCTTACTAAAGGCCACAGTTA
CCAAGGAACTCGAACATGGCGGTCGAATGATTCACTTTGACTATGACGGCATCTTTATGGAAATCCTCGATAAGTTAGGC
GAGACGCCATTGCCGCCTTACATCAAGGAAAAGCTTGAAGATCCAGAAATGTATCAAACTGTTTATTCTAAAGAGTTAGG
CTCTGCAGCGGCACCGACAGCCGGTCTTCACTGGACCAAAGATTTGCTGAAAAAAGTGCAAGAAAAGGGCGTCAAGTTGG
TTTACTTGACGTTGCATGTTGGCTTGGGTACTTTCCGGCCAGTAGTTGAGGAAAACATCGAAGCACATAAAATGCACAGT
GAATTTTATCGGTTGACTCCGGAAGCAGCAGCGACACTGAATGATGTTCGCGACAATGGCGGTCGAATTATTGCTACCGG
TACGACATCCATTCGCACCCTCGAGGCCATCGGTACCAAATTTGATGGTGCCATCAAGGCAGATTCGGGTTGGACTGACA
TTTTTATCAAGCCAGGGTATCAGTGGAAGGTTGTTGATGCATTTATCACCAACTTCCATTTGCCAAAGTCAACGTTAGTT
ATGCTCGTGGCAGCGTTCACCGGGCGCGATACAATTTTAAATGCGTATAAGCATGCGATCGACGAAAAATATCGCTTCTT
CAGCTTTGGCGATGCGATGTTCATTTACTAA

Upstream 100 bases:

>100_bases
TGGCCAAAATTCAGCCATTTAGGCTTCAAGCCCACTTATGCTCCGACTTCTAAGCGCTCCTGCTTGCGCTCACTGATTAA
AATGAATGGAAGCGAATATG

Downstream 100 bases:

>100_bases
TCACGAAGCTAGTTTCTGCTCACTTTAGTGTGTTTACTAGCCCGGCAACTTGTCTGTAAAGAGCTTAGCCGCTTACTTTC
CGGGTGTTAACCGCTGGCTT

Product: S-adenosylmethionine:tRNA-ribosyltransferase- isomerase (queuine synthetase)

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MQENKRYHKVEDKHYQLSDFDYDLPHELIAQTPLKERDASRLLVLNSETGEMQDKHFYDILDYFKPGDALVMNDTRVMPA
RLYGVKPETGAHMEVLLLNNTSGDDWETLMKPARKAPVGTVIDFGDGLLKATVTKELEHGGRMIHFDYDGIFMEILDKLG
ETPLPPYIKEKLEDPEMYQTVYSKELGSAAAPTAGLHWTKDLLKKVQEKGVKLVYLTLHVGLGTFRPVVEENIEAHKMHS
EFYRLTPEAAATLNDVRDNGGRIIATGTTSIRTLEAIGTKFDGAIKADSGWTDIFIKPGYQWKVVDAFITNFHLPKSTLV
MLVAAFTGRDTILNAYKHAIDEKYRFFSFGDAMFIY

Sequences:

>Translated_356_residues
MQENKRYHKVEDKHYQLSDFDYDLPHELIAQTPLKERDASRLLVLNSETGEMQDKHFYDILDYFKPGDALVMNDTRVMPA
RLYGVKPETGAHMEVLLLNNTSGDDWETLMKPARKAPVGTVIDFGDGLLKATVTKELEHGGRMIHFDYDGIFMEILDKLG
ETPLPPYIKEKLEDPEMYQTVYSKELGSAAAPTAGLHWTKDLLKKVQEKGVKLVYLTLHVGLGTFRPVVEENIEAHKMHS
EFYRLTPEAAATLNDVRDNGGRIIATGTTSIRTLEAIGTKFDGAIKADSGWTDIFIKPGYQWKVVDAFITNFHLPKSTLV
MLVAAFTGRDTILNAYKHAIDEKYRFFSFGDAMFIY
>Mature_356_residues
MQENKRYHKVEDKHYQLSDFDYDLPHELIAQTPLKERDASRLLVLNSETGEMQDKHFYDILDYFKPGDALVMNDTRVMPA
RLYGVKPETGAHMEVLLLNNTSGDDWETLMKPARKAPVGTVIDFGDGLLKATVTKELEHGGRMIHFDYDGIFMEILDKLG
ETPLPPYIKEKLEDPEMYQTVYSKELGSAAAPTAGLHWTKDLLKKVQEKGVKLVYLTLHVGLGTFRPVVEENIEAHKMHS
EFYRLTPEAAATLNDVRDNGGRIIATGTTSIRTLEAIGTKFDGAIKADSGWTDIFIKPGYQWKVVDAFITNFHLPKSTLV
MLVAAFTGRDTILNAYKHAIDEKYRFFSFGDAMFIY

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=347, Percent_Identity=46.9740634005764, Blast_Score=286, Evalue=1e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 40376; Mature: 40376

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQENKRYHKVEDKHYQLSDFDYDLPHELIAQTPLKERDASRLLVLNSETGEMQDKHFYDI
CCCCCCCCCCCCCCEEECCCCCCCCHHHHHCCCCCCCCCCEEEEEECCCCCCCHHHHHHH
LDYFKPGDALVMNDTRVMPARLYGVKPETGAHMEVLLLNNTSGDDWETLMKPARKAPVGT
HHHCCCCCEEEEECCEECCHHEECCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCE
VIDFGDGLLKATVTKELEHGGRMIHFDYDGIFMEILDKLGETPLPPYIKEKLEDPEMYQT
EEECCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCCCHHHHHHCCCHHHHHH
VYSKELGSAAAPTAGLHWTKDLLKKVQEKGVKLVYLTLHVGLGTFRPVVEENIEAHKMHS
HHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCHHHHHHHHCHHHHHHHH
EFYRLTPEAAATLNDVRDNGGRIIATGTTSIRTLEAIGTKFDGAIKADSGWTDIFIKPGY
HHHHCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCCCCEEECCCCCEEEEEECCC
QWKVVDAFITNFHLPKSTLVMLVAAFTGRDTILNAYKHAIDEKYRFFSFGDAMFIY
CEEEHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCEEEEEECCEEEEC
>Mature Secondary Structure
MQENKRYHKVEDKHYQLSDFDYDLPHELIAQTPLKERDASRLLVLNSETGEMQDKHFYDI
CCCCCCCCCCCCCCEEECCCCCCCCHHHHHCCCCCCCCCCEEEEEECCCCCCCHHHHHHH
LDYFKPGDALVMNDTRVMPARLYGVKPETGAHMEVLLLNNTSGDDWETLMKPARKAPVGT
HHHCCCCCEEEEECCEECCHHEECCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCE
VIDFGDGLLKATVTKELEHGGRMIHFDYDGIFMEILDKLGETPLPPYIKEKLEDPEMYQT
EEECCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCCCHHHHHHCCCHHHHHH
VYSKELGSAAAPTAGLHWTKDLLKKVQEKGVKLVYLTLHVGLGTFRPVVEENIEAHKMHS
HHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEECCCHHHHHHHHCHHHHHHHH
EFYRLTPEAAATLNDVRDNGGRIIATGTTSIRTLEAIGTKFDGAIKADSGWTDIFIKPGY
HHHHCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCCCCEEECCCCCEEEEEECCC
QWKVVDAFITNFHLPKSTLVMLVAAFTGRDTILNAYKHAIDEKYRFFSFGDAMFIY
CEEEHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCEEEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12566566 [H]