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The map label for this gene is manX [C]

Identifier: 116494116

GI number: 116494116

Start: 551921

End: 552415

Strand: Direct

Name: manX [C]

Synonym: LSEI_0560

Alternate gene names: 116494116

Gene position: 551921-552415 (Clockwise)

Preceding gene: 116494115

Following gene: 116494117

Centisome position: 19.06

GC content: 44.65

Gene sequence:

>495_bases
ATGGCAATTTCGTTTGTACGTATTGATGATCGCATGATTCATGGCTTGATCACGGTTCGTTGGGGCAAGGAATACCCGAT
GGATGGGATTATTGCGGTAAACGACAAAGCGGCAAACAATCCGATTTTGAAGGAAGCCTATATGGCAGCGTCTGACAAGA
AGACCTTCGTTTGGACGTTAGATCATTTTGACAAGGTTAAGGATAAGGTGTTGAACTCAGCAACCAAGTATTTTCTCATC
ACCAAGAGTCCTCAAGATATGAAAAAGATCTTGGTCGATATGAACTTCAAGCCGGGCGACATTAAAACAGTTGTTGTCGG
TCCAGGTAATGATCGCGACAACGCGGTCAAACTCGGAGACAATCAGTCCTTTACCCAAGAGGAAGGCGATGCCTTTGAAG
CGATTGAGAAGGCTGGTTATAAAGTTGAGTTTGCGCTGTTGCCAGATCAGCGGATTGGTAGTTGGGACAAATTCAAGTCA
CGCTTTGGGTATTAA

Upstream 100 bases:

>100_bases
AAAAGAAAAAGCATTTTCAGAAGCAACCGCGGCCATCAAAGAATTTCAGACGACCTCATCAGAAGCTTCTGACGATGACG
ATATTTAAGGAGTGAGCAAA

Downstream 100 bases:

>100_bases
TGGTTGAACAATAACGCGCTCGCAAAAAAGGAAGGTAAGAAAAATGACAATTAGTTGGTTTCAAGCAGCTATTTTAGGTG
TTTTCGCTTGCTTATGTTCC

Product: phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IIB

Products: Protein Histidine; Sugar Phosphate.; D-glucosamine-6-phosphate [Cytoplasm]; pyruvate; glucose-6-phosphate [Cytoplasm]; N-acetyl-D-glucosamine-6-phosphate [Cytoplasm]; mannose-6-phosphate [Cytoplasm]; fructose-6-phosphate [Cytoplasm] [C]

Alternate protein names: PTS System Protein; PTS System Fructose(Mannose)-Specific IIB; PTS System Mannose-Specific IIB Component; PTS System Sorbose Subfamily Transporter Subunit IIB; Pts System Mannose-Specific Eiiab Component; PTS System Fructose/Mannose-Specific IIB Component; Phosphotransferase System PTS; PTS System; LevB Protein

Number of amino acids: Translated: 164; Mature: 163

Protein sequence:

>164_residues
MAISFVRIDDRMIHGLITVRWGKEYPMDGIIAVNDKAANNPILKEAYMAASDKKTFVWTLDHFDKVKDKVLNSATKYFLI
TKSPQDMKKILVDMNFKPGDIKTVVVGPGNDRDNAVKLGDNQSFTQEEGDAFEAIEKAGYKVEFALLPDQRIGSWDKFKS
RFGY

Sequences:

>Translated_164_residues
MAISFVRIDDRMIHGLITVRWGKEYPMDGIIAVNDKAANNPILKEAYMAASDKKTFVWTLDHFDKVKDKVLNSATKYFLI
TKSPQDMKKILVDMNFKPGDIKTVVVGPGNDRDNAVKLGDNQSFTQEEGDAFEAIEKAGYKVEFALLPDQRIGSWDKFKS
RFGY
>Mature_163_residues
AISFVRIDDRMIHGLITVRWGKEYPMDGIIAVNDKAANNPILKEAYMAASDKKTFVWTLDHFDKVKDKVLNSATKYFLIT
KSPQDMKKILVDMNFKPGDIKTVVVGPGNDRDNAVKLGDNQSFTQEEGDAFEAIEKAGYKVEFALLPDQRIGSWDKFKSR
FGY

Specific function: This Is A Component Of The Phosphoenolpyruvate-Dependent Sugar Phosphotransferase System (Pts), A Major Carbohydrate Active -Transport System. The IIcd Domains Contain The Sugar Binding Site And The Transmembrane Channel; The IIa Domain Contains The Prim

COG id: COG3444

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IIB

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.1.69 [C]

Molecular weight: Translated: 18602; Mature: 18470

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: PS51101 PTS_EIIB_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAISFVRIDDRMIHGLITVRWGKEYPMDGIIAVNDKAANNPILKEAYMAASDKKTFVWTL
CEEEEEEECCCEEEEEEEEEECCCCCCCCEEEECCCCCCCCHHHHHHHHCCCCCEEEEEH
DHFDKVKDKVLNSATKYFLITKSPQDMKKILVDMNFKPGDIKTVVVGPGNDRDNAVKLGD
HHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEECC
NQSFTQEEGDAFEAIEKAGYKVEFALLPDQRIGSWDKFKSRFGY
CCCCCCCCCHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHCCC
>Mature Secondary Structure 
AISFVRIDDRMIHGLITVRWGKEYPMDGIIAVNDKAANNPILKEAYMAASDKKTFVWTL
EEEEEEECCCEEEEEEEEEECCCCCCCCEEEECCCCCCCCHHHHHHHHCCCCCEEEEEH
DHFDKVKDKVLNSATKYFLITKSPQDMKKILVDMNFKPGDIKTVVVGPGNDRDNAVKLGD
HHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEECC
NQSFTQEEGDAFEAIEKAGYKVEFALLPDQRIGSWDKFKSRFGY
CCCCCCCCCHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protein N-Phosphohistidine; Sugar; phosphoenolpyruvate; glucosamine [Periplasm]; phosphoenolpyruvate; beta-D-glucose [Periplasm]; N-acetyl-D-glucosamine [Periplasm]; mannose [Periplasm]; fructose [Periplasm] [C]

Specific reaction: Protein N-Phosphohistidine + Sugar = Protein Histidine + Sugar Phosphate. phosphoenolpyruvate + glucosamine [Periplasm] = D-glucosamine-6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + beta-D-glucose [Periplasm] = glucose-6-phosphate [Cytoplasm]

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA