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The map label for this gene is manX [C]

Identifier: 116494115

GI number: 116494115

Start: 551471

End: 551908

Strand: Direct

Name: manX [C]

Synonym: LSEI_0559

Alternate gene names: 116494115

Gene position: 551471-551908 (Clockwise)

Preceding gene: 116494113

Following gene: 116494116

Centisome position: 19.05

GC content: 44.75

Gene sequence:

>438_bases
ATGAAATATTTGCTTCTTGTCAGTCACGGTGATTTCTCCAGTGGCTTAAAGCAAACCTTGGGTATGTTTGCGGGCGATGA
TGCGATTGGTTCTGTCATTGCGGTTGGTTTAAAACCCGATGAAGCAGCTTCAACATTTGGAACACGGTTTGAGGCATTGC
TAAACACCCTGCCAGAGGATGCAAGCTTTGTTGTTCTGGCAGATATTGTCGGCGGTAGTCCACTCACAACGGTTTGCAAC
ATTTTGAACGACCATGGCAAGCTGCAAGATACGCTTGTTCTTGGCGGGATGAACTTCCCAATGGCGTTAACCACATTGAT
GTCGAAAGACAGTTTAGATAACTCAGCGTTAAAAGAAAAAGCATTTTCAGAAGCAACCGCGGCCATCAAAGAATTTCAGA
CGACCTCATCAGAAGCTTCTGACGATGACGATATTTAA

Upstream 100 bases:

>100_bases
TGTCCGAACAATAATGGATCACTTTCAGTTGTACTTCGGTTGATTGTCGCCTTTTGCGGTAAATCCATATTTTCATAGTC
TTGATAAAGGGATGATTTAG

Downstream 100 bases:

>100_bases
GGAGTGAGCAAAATGGCAATTTCGTTTGTACGTATTGATGATCGCATGATTCATGGCTTGATCACGGTTCGTTGGGGCAA
GGAATACCCGATGGATGGGA

Product: phosphotransferase system, mannose/fructose-specific component IIA

Products: Protein Histidine; Sugar Phosphate.; D-glucosamine-6-phosphate [Cytoplasm]; pyruvate; glucose-6-phosphate [Cytoplasm]; N-acetyl-D-glucosamine-6-phosphate [Cytoplasm]; mannose-6-phosphate [Cytoplasm]; fructose-6-phosphate [Cytoplasm] [C]

Alternate protein names: PTS System Sugar-Specific IIA Component; LevA Protein; PTS System Mannose-Specific IIAB Component; PTS System Fructose(Mannose)-Specific IIA Component; PTS System; PTS System Fructose Family Transporter Subunit IIA

Number of amino acids: Translated: 145; Mature: 145

Protein sequence:

>145_residues
MKYLLLVSHGDFSSGLKQTLGMFAGDDAIGSVIAVGLKPDEAASTFGTRFEALLNTLPEDASFVVLADIVGGSPLTTVCN
ILNDHGKLQDTLVLGGMNFPMALTTLMSKDSLDNSALKEKAFSEATAAIKEFQTTSSEASDDDDI

Sequences:

>Translated_145_residues
MKYLLLVSHGDFSSGLKQTLGMFAGDDAIGSVIAVGLKPDEAASTFGTRFEALLNTLPEDASFVVLADIVGGSPLTTVCN
ILNDHGKLQDTLVLGGMNFPMALTTLMSKDSLDNSALKEKAFSEATAAIKEFQTTSSEASDDDDI
>Mature_145_residues
MKYLLLVSHGDFSSGLKQTLGMFAGDDAIGSVIAVGLKPDEAASTFGTRFEALLNTLPEDASFVVLADIVGGSPLTTVCN
ILNDHGKLQDTLVLGGMNFPMALTTLMSKDSLDNSALKEKAFSEATAAIKEFQTTSSEASDDDDI

Specific function: This Is A Component Of The Phosphoenolpyruvate-Dependent Sugar Phosphotransferase System (Pts), A Major Carbohydrate Active -Transport System. The IIcd Domains Contain The Sugar Binding Site And The Transmembrane Channel; The IIa Domain Contains The Prim

COG id: COG2893

COG function: function code G; Phosphotransferase system, mannose/fructose-specific component IIA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.1.69 [C]

Molecular weight: Translated: 15237; Mature: 15237

Theoretical pI: Translated: 4.03; Mature: 4.03

Prosite motif: PS51096 PTS_EIIA_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYLLLVSHGDFSSGLKQTLGMFAGDDAIGSVIAVGLKPDEAASTFGTRFEALLNTLPED
CCEEEEEECCCHHHHHHHHHHHHCCCHHHHHEEEECCCCCHHHHHHHHHHHHHHHHCCCC
ASFVVLADIVGGSPLTTVCNILNDHGKLQDTLVLGGMNFPMALTTLMSKDSLDNSALKEK
CCEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCCHHHHHHH
AFSEATAAIKEFQTTSSEASDDDDI
HHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKYLLLVSHGDFSSGLKQTLGMFAGDDAIGSVIAVGLKPDEAASTFGTRFEALLNTLPED
CCEEEEEECCCHHHHHHHHHHHHCCCHHHHHEEEECCCCCHHHHHHHHHHHHHHHHCCCC
ASFVVLADIVGGSPLTTVCNILNDHGKLQDTLVLGGMNFPMALTTLMSKDSLDNSALKEK
CCEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCCHHHHHHH
AFSEATAAIKEFQTTSSEASDDDDI
HHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protein N-Phosphohistidine; Sugar; phosphoenolpyruvate; glucosamine [Periplasm]; phosphoenolpyruvate; beta-D-glucose [Periplasm]; N-acetyl-D-glucosamine [Periplasm]; mannose [Periplasm]; fructose [Periplasm] [C]

Specific reaction: Protein N-Phosphohistidine + Sugar = Protein Histidine + Sugar Phosphate. phosphoenolpyruvate + glucosamine [Periplasm] = D-glucosamine-6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + beta-D-glucose [Periplasm] = glucose-6-phosphate [Cytoplasm]

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA