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The map label for this gene is rihC [H]

Identifier: 116493454

GI number: 116493454

Start: 1701210

End: 1702124

Strand: Reverse

Name: rihC [H]

Synonym: PEPE_1726

Alternate gene names: 116493454

Gene position: 1702124-1701210 (Counterclockwise)

Preceding gene: 116493455

Following gene: 116493452

Centisome position: 92.89

GC content: 41.64

Gene sequence:

>915_bases
ATGACAACAAAAATTATTATGGATACAGATCCAGGAATTGATGATGCAGCTGCGATTTCCGTGGCCATCAATAATCCTGA
AATCGAATTAGAATTAATTACGACGGTGGCTGGGAATGTTACGGTTGATAAAACTACTAAGAACGCGATGAAGTTAGTAA
AATTCTTCAACGCTGATATTCCGGTTGCTGGTGGGGCAGAACAACCTTTGATTAAACCATTTGAAGATGCTGCTCGAGTT
CACGGCGAATCCGGGATGCCTGGTTACGATTTTGGTGCAGAACCCACTAACATTCCAGTTAACAATGCAGTGGATGTTTT
ATATCACACTATTATGGCTAACGATGAACCGATCGTTTTGGTACCCACGGGATCGTATACTAACATTGCAGTACTACTTA
CTGAACATCCCGATGTAAAAACCAAGATCGAAAAAATTGTGGCCATGGGTGGCGCAATTGGCATGGGAAATATGACAAGC
GCTGCAGAATTCAACGTCTTTACTGATCCCCACGCTGCTGAAATTTTATATAAGAGTGGCATTCCAGTAGTAATGGTTGG
CCTAGATGTAACGATGAAAGCTCTTCTAACACAATCTACGTTAGACACAGTAAAATCCTTAGGTAAAGCTGGAGAAATGC
TGGGAGCCATTTTTAACCACTATTACGAGGGAAATGAAGGTGGTATCCCAATGCATGATGTTAATACGTTGGCATACTTA
CTAAAACCCGAAATGTATAAGACCATCCCATATTGGATTGACGTAGTTACTGAAGGACCGGCGAATGGTGAAACCGTTGC
AGATATCCGAGGTGCTTATCACGATGGAAAGACCAACGCTGAAGTGTGTGTTGATATTGATGCCGACGCCTTCAATGCCT
GGTTTATTGAAGAAGTTAAAAAGATGAATGCTTAA

Upstream 100 bases:

>100_bases
ATTAATTAATTTAAATAGATAGTGAAAAAGAATGGACGTAACGTGCATTCTTTTTTAGAATGGTAATGAGGACGCTTTCA
TAAAAGCTGGAGGTAAGAAA

Downstream 100 bases:

>100_bases
TTTAAATGAATAAAAGTCCCATGATGATTAAAGCTTAGTTTTTCCTAAGCCAATCATCATGGGATTTTTTTAAACCTTTA
AGGCTCGTTTTAACAAGATT

Product: ribonucleoside hydrolase RihC

Products: D-ribose; purine

Alternate protein names: Purine/pyrimidine ribonucleoside hydrolase [H]

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MTTKIIMDTDPGIDDAAAISVAINNPEIELELITTVAGNVTVDKTTKNAMKLVKFFNADIPVAGGAEQPLIKPFEDAARV
HGESGMPGYDFGAEPTNIPVNNAVDVLYHTIMANDEPIVLVPTGSYTNIAVLLTEHPDVKTKIEKIVAMGGAIGMGNMTS
AAEFNVFTDPHAAEILYKSGIPVVMVGLDVTMKALLTQSTLDTVKSLGKAGEMLGAIFNHYYEGNEGGIPMHDVNTLAYL
LKPEMYKTIPYWIDVVTEGPANGETVADIRGAYHDGKTNAEVCVDIDADAFNAWFIEEVKKMNA

Sequences:

>Translated_304_residues
MTTKIIMDTDPGIDDAAAISVAINNPEIELELITTVAGNVTVDKTTKNAMKLVKFFNADIPVAGGAEQPLIKPFEDAARV
HGESGMPGYDFGAEPTNIPVNNAVDVLYHTIMANDEPIVLVPTGSYTNIAVLLTEHPDVKTKIEKIVAMGGAIGMGNMTS
AAEFNVFTDPHAAEILYKSGIPVVMVGLDVTMKALLTQSTLDTVKSLGKAGEMLGAIFNHYYEGNEGGIPMHDVNTLAYL
LKPEMYKTIPYWIDVVTEGPANGETVADIRGAYHDGKTNAEVCVDIDADAFNAWFIEEVKKMNA
>Mature_303_residues
TTKIIMDTDPGIDDAAAISVAINNPEIELELITTVAGNVTVDKTTKNAMKLVKFFNADIPVAGGAEQPLIKPFEDAARVH
GESGMPGYDFGAEPTNIPVNNAVDVLYHTIMANDEPIVLVPTGSYTNIAVLLTEHPDVKTKIEKIVAMGGAIGMGNMTSA
AEFNVFTDPHAAEILYKSGIPVVMVGLDVTMKALLTQSTLDTVKSLGKAGEMLGAIFNHYYEGNEGGIPMHDVNTLAYLL
KPEMYKTIPYWIDVVTEGPANGETVADIRGAYHDGKTNAEVCVDIDADAFNAWFIEEVKKMNA

Specific function: Hydrolyzes both purine and pyrimidine ribonucleosides with a broad-substrate specificity [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihC subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786213, Length=303, Percent_Identity=46.8646864686469, Blast_Score=256, Evalue=1e-69,
Organism=Escherichia coli, GI1786871, Length=311, Percent_Identity=42.7652733118971, Blast_Score=232, Evalue=2e-62,
Organism=Escherichia coli, GI1788486, Length=308, Percent_Identity=39.9350649350649, Blast_Score=197, Evalue=8e-52,
Organism=Caenorhabditis elegans, GI32565474, Length=191, Percent_Identity=34.0314136125654, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17565698, Length=198, Percent_Identity=30.3030303030303, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320608, Length=257, Percent_Identity=32.295719844358, Blast_Score=102, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24583915, Length=201, Percent_Identity=29.3532338308458, Blast_Score=81, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24641839, Length=188, Percent_Identity=30.8510638297872, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022976 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: 3.2.2.1

Molecular weight: Translated: 32621; Mature: 32489

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTKIIMDTDPGIDDAAAISVAINNPEIELELITTVAGNVTVDKTTKNAMKLVKFFNADI
CCEEEEEECCCCCCCCEEEEEEECCCCEEEEEEEEECCCEEEECCHHHHHHHHHHHCCCC
PVAGGAEQPLIKPFEDAARVHGESGMPGYDFGAEPTNIPVNNAVDVLYHTIMANDEPIVL
CCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEE
VPTGSYTNIAVLLTEHPDVKTKIEKIVAMGGAIGMGNMTSAAEFNVFTDPHAAEILYKSG
EECCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHCC
IPVVMVGLDVTMKALLTQSTLDTVKSLGKAGEMLGAIFNHYYEGNEGGIPMHDVNTLAYL
CCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
LKPEMYKTIPYWIDVVTEGPANGETVADIRGAYHDGKTNAEVCVDIDADAFNAWFIEEVK
HCCHHHHHCCEEEEEEECCCCCCCCHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHH
KMNA
HHCC
>Mature Secondary Structure 
TTKIIMDTDPGIDDAAAISVAINNPEIELELITTVAGNVTVDKTTKNAMKLVKFFNADI
CEEEEEECCCCCCCCEEEEEEECCCCEEEEEEEEECCCEEEECCHHHHHHHHHHHCCCC
PVAGGAEQPLIKPFEDAARVHGESGMPGYDFGAEPTNIPVNNAVDVLYHTIMANDEPIVL
CCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEE
VPTGSYTNIAVLLTEHPDVKTKIEKIVAMGGAIGMGNMTSAAEFNVFTDPHAAEILYKSG
EECCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHCC
IPVVMVGLDVTMKALLTQSTLDTVKSLGKAGEMLGAIFNHYYEGNEGGIPMHDVNTLAYL
CCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
LKPEMYKTIPYWIDVVTEGPANGETVADIRGAYHDGKTNAEVCVDIDADAFNAWFIEEVK
HCCHHHHHCCEEEEEEECCCCCCCCHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHH
KMNA
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-D-ribosylpurine; H2O

Specific reaction: an N-D-ribosylpurine + H2O = D-ribose + a purine

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA