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The map label for this gene is nupG [H]

Identifier: 116493455

GI number: 116493455

Start: 1702243

End: 1703511

Strand: Reverse

Name: nupG [H]

Synonym: PEPE_1727

Alternate gene names: 116493455

Gene position: 1703511-1702243 (Counterclockwise)

Preceding gene: 116493456

Following gene: 116493454

Centisome position: 92.97

GC content: 38.14

Gene sequence:

>1269_bases
ATGTTTTTAGCAATAAATATTATTGGTATTATTGTATTTCTAGGCGTCGCTGTTCTATTTTCAAAAGACCGTAAACATAT
CAACTGGGCGTCGGTTGGTATTATGGTAGTGATCAACATTCTGTTAGCTTGGTTCTTAGTTAACTCGTCTGTTGGTATTA
ACGGGGTTAAAGCCGCTGCTGATGGCTTTAAAGCTTTGGTTGATGTTTCTTATAAAGGTATTGAATTTGCGTTGCCTGAT
TGGGTACACGTTAAATCAATGAACTTTGTTACAAGTGCATTGCTCCCAATTTTGATGATTGTTCCATTGTTTGATATTTT
AACTTACATTGGTATCCTTCCATGGATTATCAAGTGGGTTGGTAAGGGTCTATCATTCATTACCCGTCAACCTAAGTTTG
AATCATTCTTCGCTGTAGAAATGATGTTTTTAGGTAACACTGAAGCTCTTGCTGTTTCAGGTTTACAACTTCGTAACATG
AAGTCACAACGTTTGGTTACAATTGCCATGATGTCAATGAGTTGTGTTACTGCATCAATTTTAGGTTCATACTTACTTAT
GATGCCGGGTCAATATATCATCGCTGCTGTTCCAATTAACATTGTTAATTCAATTATTGTTACAACAGTTTTGAATCCAG
TTACTGTAACACCAGAAGAAGATACAATTGCTAAGATTGGCGGAAGTGCCTCTGGCGAAAGTACAGATACCATCGAATCT
GGTTCTGAAGGGGTTGCTGAAGAAACAGTTAGCAAGAAGCCTGAACGCGAACCATTTTTCTCATTCTTGGGCGACTCAAT
TTTGGGCGCTGGTCGTTTGATCTTAATTATCACAGCTAACGTAATTGCCTTTGTTGCTTTGGCTGCTTTGATTGATGCTT
TACTAGGTAAGATTAACCCAGTTCTTTCTCTAGAAAACATTCTTGGTTGTGTAATGTTCCCATTTGCATGGTTATTAGGT
TTCGACCCTAGTCATGCTTTCCAAATGGCTCAATATATGGGTACCAAGTTAGTTACCAATGAATTTGTAGTTATGGGTGA
TGTAACTGGTAAGATGAAAGATTTCGCACCTCATTTTGCTGCAACATTGACCGTCTTCTTAACTTCATTTGCTAACTTCT
CAACCACAGGGATGATCATCGGTGCTTTCAAGGGTCTTGTCGATCGTGAAAGAAATGATGCAATTTCTAAGAATGTTGGT
TACATGCTTCTTTCCGGTATTTTAGTTTCATTACTATCAGCTGGATTTGTAGGCGTGTTCGTCTGGTAA

Upstream 100 bases:

>100_bases
TTTAAAAATCTTTATATTTTGTATTTACAGACTAATATTTAGTAGTTATTATTATTACAACGTCTGTTACATAATTTGGA
TCATAGAGGGAGATTTCGGC

Downstream 100 bases:

>100_bases
CTTTTTTAACAGTTAATTATTAATTAATTTAAATAGATAGTGAAAAAGAATGGACGTAACGTGCATTCTTTTTTAGAATG
GTAATGAGGACGCTTTCATA

Product: nucleoside permease

Products: uridine [Cytoplasm]; thymidine [Cytoplasm]; inosine [Cytoplasm]; Deoxyuridine [Cytoplasm]; deoxyinosine [Cytoplasm]; deoxycytidine [Cytoplasm]; deoxyadenosine [Cytoplasm]; cytidine [Cytoplasm]; Proton [Cytoplasm]; adenosine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MFLAINIIGIIVFLGVAVLFSKDRKHINWASVGIMVVINILLAWFLVNSSVGINGVKAAADGFKALVDVSYKGIEFALPD
WVHVKSMNFVTSALLPILMIVPLFDILTYIGILPWIIKWVGKGLSFITRQPKFESFFAVEMMFLGNTEALAVSGLQLRNM
KSQRLVTIAMMSMSCVTASILGSYLLMMPGQYIIAAVPINIVNSIIVTTVLNPVTVTPEEDTIAKIGGSASGESTDTIES
GSEGVAEETVSKKPEREPFFSFLGDSILGAGRLILIITANVIAFVALAALIDALLGKINPVLSLENILGCVMFPFAWLLG
FDPSHAFQMAQYMGTKLVTNEFVVMGDVTGKMKDFAPHFAATLTVFLTSFANFSTTGMIIGAFKGLVDRERNDAISKNVG
YMLLSGILVSLLSAGFVGVFVW

Sequences:

>Translated_422_residues
MFLAINIIGIIVFLGVAVLFSKDRKHINWASVGIMVVINILLAWFLVNSSVGINGVKAAADGFKALVDVSYKGIEFALPD
WVHVKSMNFVTSALLPILMIVPLFDILTYIGILPWIIKWVGKGLSFITRQPKFESFFAVEMMFLGNTEALAVSGLQLRNM
KSQRLVTIAMMSMSCVTASILGSYLLMMPGQYIIAAVPINIVNSIIVTTVLNPVTVTPEEDTIAKIGGSASGESTDTIES
GSEGVAEETVSKKPEREPFFSFLGDSILGAGRLILIITANVIAFVALAALIDALLGKINPVLSLENILGCVMFPFAWLLG
FDPSHAFQMAQYMGTKLVTNEFVVMGDVTGKMKDFAPHFAATLTVFLTSFANFSTTGMIIGAFKGLVDRERNDAISKNVG
YMLLSGILVSLLSAGFVGVFVW
>Mature_422_residues
MFLAINIIGIIVFLGVAVLFSKDRKHINWASVGIMVVINILLAWFLVNSSVGINGVKAAADGFKALVDVSYKGIEFALPD
WVHVKSMNFVTSALLPILMIVPLFDILTYIGILPWIIKWVGKGLSFITRQPKFESFFAVEMMFLGNTEALAVSGLQLRNM
KSQRLVTIAMMSMSCVTASILGSYLLMMPGQYIIAAVPINIVNSIIVTTVLNPVTVTPEEDTIAKIGGSASGESTDTIES
GSEGVAEETVSKKPEREPFFSFLGDSILGAGRLILIITANVIAFVALAALIDALLGKINPVLSLENILGCVMFPFAWLLG
FDPSHAFQMAQYMGTKLVTNEFVVMGDVTGKMKDFAPHFAATLTVFLTSFANFSTTGMIIGAFKGLVDRERNDAISKNVG
YMLLSGILVSLLSAGFVGVFVW

Specific function: Involved in the uptake of the purine ribonucleosides inosine and guanosine [H]

COG id: COG1972

COG function: function code F; Nucleoside permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the concentrative nucleoside transporter (CNT) (TC 2.A.41) family [H]

Homologues:

Organism=Homo sapiens, GI11545853, Length=354, Percent_Identity=23.728813559322, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI42542381, Length=440, Percent_Identity=22.9545454545455, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI227116277, Length=444, Percent_Identity=21.8468468468468, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1788737, Length=403, Percent_Identity=33.9950372208437, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1788488, Length=443, Percent_Identity=26.6365688487585, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788485, Length=435, Percent_Identity=25.2873563218391, Blast_Score=97, Evalue=2e-21,
Organism=Drosophila melanogaster, GI45552519, Length=429, Percent_Identity=22.6107226107226, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI45552517, Length=429, Percent_Identity=22.6107226107226, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI19921868, Length=429, Percent_Identity=22.6107226107226, Blast_Score=79, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008276
- InterPro:   IPR018270
- InterPro:   IPR011657
- InterPro:   IPR002668 [H]

Pfam domain/function: PF07662 Nucleos_tra2_C; PF01773 Nucleos_tra2_N [H]

EC number: NA

Molecular weight: Translated: 45565; Mature: 45565

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLAINIIGIIVFLGVAVLFSKDRKHINWASVGIMVVINILLAWFLVNSSVGINGVKAAA
CEEEHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
DGFKALVDVSYKGIEFALPDWVHVKSMNFVTSALLPILMIVPLFDILTYIGILPWIIKWV
HHHHHHHHCCCCCEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GKGLSFITRQPKFESFFAVEMMFLGNTEALAVSGLQLRNMKSQRLVTIAMMSMSCVTASI
HCCHHHHHCCCCCHHHHHHHHHEECCCHHHEECCHHHHCCCHHHHHHHHHHHHHHHHHHH
LGSYLLMMPGQYIIAAVPINIVNSIIVTTVLNPVTVTPEEDTIAKIGGSASGESTDTIES
HHHHHHHCCCCCEEEEECHHHHHHHHHHHHCCCEEECCCCCHHHHCCCCCCCCCCCCCCC
GSEGVAEETVSKKPEREPFFSFLGDSILGAGRLILIITANVIAFVALAALIDALLGKINP
CCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCH
VLSLENILGCVMFPFAWLLGFDPSHAFQMAQYMGTKLVTNEFVVMGDVTGKMKDFAPHFA
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHCCCCEEEEECCCCHHHHHHHHHH
ATLTVFLTSFANFSTTGMIIGAFKGLVDRERNDAISKNVGYMLLSGILVSLLSAGFVGVF
HHHHHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEE
VW
EC
>Mature Secondary Structure
MFLAINIIGIIVFLGVAVLFSKDRKHINWASVGIMVVINILLAWFLVNSSVGINGVKAAA
CEEEHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
DGFKALVDVSYKGIEFALPDWVHVKSMNFVTSALLPILMIVPLFDILTYIGILPWIIKWV
HHHHHHHHCCCCCEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GKGLSFITRQPKFESFFAVEMMFLGNTEALAVSGLQLRNMKSQRLVTIAMMSMSCVTASI
HCCHHHHHCCCCCHHHHHHHHHEECCCHHHEECCHHHHCCCHHHHHHHHHHHHHHHHHHH
LGSYLLMMPGQYIIAAVPINIVNSIIVTTVLNPVTVTPEEDTIAKIGGSASGESTDTIES
HHHHHHHCCCCCEEEEECHHHHHHHHHHHHCCCEEECCCCCHHHHCCCCCCCCCCCCCCC
GSEGVAEETVSKKPEREPFFSFLGDSILGAGRLILIITANVIAFVALAALIDALLGKINP
CCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCH
VLSLENILGCVMFPFAWLLGFDPSHAFQMAQYMGTKLVTNEFVVMGDVTGKMKDFAPHFA
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHCCCCEEEEECCCCHHHHHHHHHH
ATLTVFLTSFANFSTTGMIIGAFKGLVDRERNDAISKNVGYMLLSGILVSLLSAGFVGVF
HHHHHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEE
VW
EC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine [Periplasm]; thymidine [Periplasm]; inosine [Periplasm]; Deoxyuridine [Periplasm]; deoxyinosine [Periplasm]; deoxycytidine [Periplasm]; deoxyadenosine [Periplasm]; cytidine [Periplasm]; Proton [Periplasm]; adenosine [Periplasm] [C]

Specific reaction: Proton [Periplasm] + uridine [Periplasm] = Proton [Cytoplasm] + uridine [Cytoplasm] Proton [Periplasm] + thymidine [Periplasm] = Proton [Cytoplasm] + thymidine [Cytoplasm] Proton [Periplasm] + inosine [Periplasm] = Proton [Cytoplasm] + inosine [Cytoplasm]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969509; 9384377 [H]