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The map label for this gene is pyk [H]

Identifier: 116492854

GI number: 116492854

Start: 1086542

End: 1088305

Strand: Reverse

Name: pyk [H]

Synonym: PEPE_1092

Alternate gene names: 116492854

Gene position: 1088305-1086542 (Counterclockwise)

Preceding gene: 116492855

Following gene: 116492852

Centisome position: 59.39

GC content: 39.29

Gene sequence:

>1764_bases
ATGAAGAAAACCAAAATCGTTTCAACACTTGGTCCTGCAAGTACTTCTGTTGACACAATTGTTAAATTAATTGAAGCCGG
TGCTAATGTATTCCGCTTTAACTTTTCACATGGTGACCATGAAGAACATCTAGGTCGTTATAACATGGTTAAAGAAGCAG
AAAAGATTACTGGCAAGTCAGTAGGTATTTTGCTTGATACAAAGGGTGCTGAAATCCGTACAACTCCACAAAAAGATGGT
AACCAAGAATATCACACAAATGATAAGGTTCGCATCTCAATGGATGACACTTTGGAAACAACAAAAGAAAAGATTGCTGT
TACATATGATGGACTTTTTGATGACGTTCATGTTGGTGGACACGTTCTTTTTGATGATGGACTTCTTGATTTTAAGATTG
ACGAAAAAGATGAAGAAAACCGCGAATTAGTTGCTCATGCAACTAACAATGGTGTTCTTGGATCACGTAAGGGTACAAAT
GCTCCTGGTGTTTCAATTAACTTACCTGGTATTACTGAAAAAGATGCTGATGATATTCGTTTTGGTCTTGAATCAATGAA
CATCAACTTCATCGCTGCAAGTTTCGTACGTAAGCCACAAGACGTTCTTGAAATTCGTGAATTGTTGGAAGAAAAGAACA
TGGAAGATGTTCAAATTTTCCCTAAGATCGAATCACAAGAAGGTATTGACAACACAGACGAAATCTTGAAAGTTTCAGAT
GGTATTATGATTGCTCGTGGTGACATGGGTGTTGAAATTCCAGCTGAAAATGTTCCTTTGGTACAAAAGACATTGATCAA
GAAGTGTAATGCAGTTGGTTTGCCTGTTATCACAGCAACTCAAATGCTTGACTCAATGATCGAAAACCCACGTCCAACAC
GTGCTGAAGCTTCTGACGTTGCTAATGCTGTTTGGGATGGTACAGATGCTACTATGCTTTCTGGTGAAAGTGCTAATGGT
GACTACCCTGTTGAAGCTGTTGCTACAATGGCTAAGATTGACGAAAAAGCTGAAAATGCTATGGCTGAAGACGGAAATCT
TCAAATCAACACATTTGATCAAAGTGATGTTACAGAAACAATTAGTGCTGCTGTTGCTCGTGCTGCTAAGAACCTTGGTG
TTAAGACTATCGTTGCTGCAACACAATCAGGTTACACAGCTCGTATGATTTCTAAGTATCGTCCAGATGCTGACATTCTT
GCAATCACATTTGACGAACGTGTTCGTCGCGGTTTGATGGTTAACTGGGGTGTACACCCAATCATCGCTGACCGTCCATC
AACAACTGATGAAATGTTTGAACTAGCTGCTAACAAAGCTGTAGATCTTGGTCTTGCTAAAGAAGGAGACTTGATTCTTG
TTGTTGCTGGTGTTCCAGTTGGCGAAAGTGGAACAACAAACATCATGAAGTTACAACTAATTGGTTCAAAGCTTGCTTCT
GGTCAAGGTGTTGGTGATGAAACAGTTATTGGTAAAGCAGTTGTTGCTACATCAGCTGATGAAGCTAACAAAAAAGCTGT
TGAAGGCGGAGTACTTGTTACAAAGACAACTGACAAAGACTATCTACCAGCAATTGAAAAATCAAGTGCTTTAGTAGTTG
AAAATGGTGGTTTGACATCACACGCTGCAGTAGTTGGTATTTCAATGGGAATTCCTGTTATTGTTGGTGTACAAAACGCA
ACTTCAATCCTTTCAGATGATGAATTGATTACAGTTGACTCACGTCGTGGTATTATCTACCATGGTGCATCAAACTCACT
ATAA

Upstream 100 bases:

>100_bases
CCAATTAAGTGAAGATTTATCATTCTAGAGTTCTATTAATATTTGGATAAAATGACTTAAGAAGTCTTTTATAATTTAAA
ATCAAGGGAGAGATTCTGTA

Downstream 100 bases:

>100_bases
AAATTTAATTTAAATTTTTAAAACCTGGAACATTATATGTTCTAGGTTTTTTTGTTTGGCAGAATGAAAGTTGAATTTAG
ACTGTTAGGGTTCTAGATTT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 587; Mature: 587

Protein sequence:

>587_residues
MKKTKIVSTLGPASTSVDTIVKLIEAGANVFRFNFSHGDHEEHLGRYNMVKEAEKITGKSVGILLDTKGAEIRTTPQKDG
NQEYHTNDKVRISMDDTLETTKEKIAVTYDGLFDDVHVGGHVLFDDGLLDFKIDEKDEENRELVAHATNNGVLGSRKGTN
APGVSINLPGITEKDADDIRFGLESMNINFIAASFVRKPQDVLEIRELLEEKNMEDVQIFPKIESQEGIDNTDEILKVSD
GIMIARGDMGVEIPAENVPLVQKTLIKKCNAVGLPVITATQMLDSMIENPRPTRAEASDVANAVWDGTDATMLSGESANG
DYPVEAVATMAKIDEKAENAMAEDGNLQINTFDQSDVTETISAAVARAAKNLGVKTIVAATQSGYTARMISKYRPDADIL
AITFDERVRRGLMVNWGVHPIIADRPSTTDEMFELAANKAVDLGLAKEGDLILVVAGVPVGESGTTNIMKLQLIGSKLAS
GQGVGDETVIGKAVVATSADEANKKAVEGGVLVTKTTDKDYLPAIEKSSALVVENGGLTSHAAVVGISMGIPVIVGVQNA
TSILSDDELITVDSRRGIIYHGASNSL

Sequences:

>Translated_587_residues
MKKTKIVSTLGPASTSVDTIVKLIEAGANVFRFNFSHGDHEEHLGRYNMVKEAEKITGKSVGILLDTKGAEIRTTPQKDG
NQEYHTNDKVRISMDDTLETTKEKIAVTYDGLFDDVHVGGHVLFDDGLLDFKIDEKDEENRELVAHATNNGVLGSRKGTN
APGVSINLPGITEKDADDIRFGLESMNINFIAASFVRKPQDVLEIRELLEEKNMEDVQIFPKIESQEGIDNTDEILKVSD
GIMIARGDMGVEIPAENVPLVQKTLIKKCNAVGLPVITATQMLDSMIENPRPTRAEASDVANAVWDGTDATMLSGESANG
DYPVEAVATMAKIDEKAENAMAEDGNLQINTFDQSDVTETISAAVARAAKNLGVKTIVAATQSGYTARMISKYRPDADIL
AITFDERVRRGLMVNWGVHPIIADRPSTTDEMFELAANKAVDLGLAKEGDLILVVAGVPVGESGTTNIMKLQLIGSKLAS
GQGVGDETVIGKAVVATSADEANKKAVEGGVLVTKTTDKDYLPAIEKSSALVVENGGLTSHAAVVGISMGIPVIVGVQNA
TSILSDDELITVDSRRGIIYHGASNSL
>Mature_587_residues
MKKTKIVSTLGPASTSVDTIVKLIEAGANVFRFNFSHGDHEEHLGRYNMVKEAEKITGKSVGILLDTKGAEIRTTPQKDG
NQEYHTNDKVRISMDDTLETTKEKIAVTYDGLFDDVHVGGHVLFDDGLLDFKIDEKDEENRELVAHATNNGVLGSRKGTN
APGVSINLPGITEKDADDIRFGLESMNINFIAASFVRKPQDVLEIRELLEEKNMEDVQIFPKIESQEGIDNTDEILKVSD
GIMIARGDMGVEIPAENVPLVQKTLIKKCNAVGLPVITATQMLDSMIENPRPTRAEASDVANAVWDGTDATMLSGESANG
DYPVEAVATMAKIDEKAENAMAEDGNLQINTFDQSDVTETISAAVARAAKNLGVKTIVAATQSGYTARMISKYRPDADIL
AITFDERVRRGLMVNWGVHPIIADRPSTTDEMFELAANKAVDLGLAKEGDLILVVAGVPVGESGTTNIMKLQLIGSKLAS
GQGVGDETVIGKAVVATSADEANKKAVEGGVLVTKTTDKDYLPAIEKSSALVVENGGLTSHAAVVGISMGIPVIVGVQNA
TSILSDDELITVDSRRGIIYHGASNSL

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI10835121, Length=490, Percent_Identity=41.2244897959184, Blast_Score=345, Evalue=9e-95,
Organism=Homo sapiens, GI33286418, Length=495, Percent_Identity=40.8080808080808, Blast_Score=344, Evalue=1e-94,
Organism=Homo sapiens, GI32967597, Length=490, Percent_Identity=41.2244897959184, Blast_Score=344, Evalue=1e-94,
Organism=Homo sapiens, GI33286422, Length=490, Percent_Identity=40.8163265306122, Blast_Score=343, Evalue=2e-94,
Organism=Homo sapiens, GI33286420, Length=490, Percent_Identity=40.8163265306122, Blast_Score=343, Evalue=2e-94,
Organism=Homo sapiens, GI310128732, Length=291, Percent_Identity=42.2680412371134, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI310128730, Length=291, Percent_Identity=42.2680412371134, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI310128736, Length=239, Percent_Identity=38.0753138075314, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI310128734, Length=239, Percent_Identity=38.0753138075314, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI310128738, Length=218, Percent_Identity=37.1559633027523, Blast_Score=132, Evalue=1e-30,
Organism=Escherichia coli, GI1787965, Length=476, Percent_Identity=46.8487394957983, Blast_Score=410, Evalue=1e-115,
Organism=Escherichia coli, GI1788160, Length=481, Percent_Identity=37.8378378378378, Blast_Score=261, Evalue=8e-71,
Organism=Escherichia coli, GI1787994, Length=165, Percent_Identity=32.1212121212121, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17544584, Length=483, Percent_Identity=40.9937888198758, Blast_Score=353, Evalue=1e-97,
Organism=Caenorhabditis elegans, GI17506829, Length=488, Percent_Identity=42.4180327868852, Blast_Score=323, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI71984413, Length=488, Percent_Identity=42.4180327868852, Blast_Score=323, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI17506831, Length=488, Percent_Identity=42.4180327868852, Blast_Score=323, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI71984406, Length=488, Percent_Identity=42.4180327868852, Blast_Score=323, Evalue=2e-88,
Organism=Saccharomyces cerevisiae, GI6319279, Length=487, Percent_Identity=40.041067761807, Blast_Score=330, Evalue=4e-91,
Organism=Saccharomyces cerevisiae, GI6324923, Length=493, Percent_Identity=40.1622718052738, Blast_Score=312, Evalue=1e-85,
Organism=Drosophila melanogaster, GI24648964, Length=488, Percent_Identity=40.3688524590164, Blast_Score=324, Evalue=1e-88,
Organism=Drosophila melanogaster, GI28571814, Length=488, Percent_Identity=40.3688524590164, Blast_Score=323, Evalue=2e-88,
Organism=Drosophila melanogaster, GI24648966, Length=414, Percent_Identity=41.7874396135266, Blast_Score=296, Evalue=2e-80,
Organism=Drosophila melanogaster, GI24581235, Length=480, Percent_Identity=34.375, Blast_Score=259, Evalue=4e-69,
Organism=Drosophila melanogaster, GI24646914, Length=327, Percent_Identity=38.5321100917431, Blast_Score=206, Evalue=4e-53,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 62845; Mature: 62845

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTKIVSTLGPASTSVDTIVKLIEAGANVFRFNFSHGDHEEHLGRYNMVKEAEKITGKS
CCCCEEEEECCCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHCCHHHHHHHHHHHCCCE
VGILLDTKGAEIRTTPQKDGNQEYHTNDKVRISMDDTLETTKEKIAVTYDGLFDDVHVGG
EEEEEECCCCEEEECCCCCCCCCCCCCCEEEEEECCHHHHCHHEEEEEECCCCCCEECCC
HVLFDDGLLDFKIDEKDEENRELVAHATNNGVLGSRKGTNAPGVSINLPGITEKDADDIR
EEEEECCEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHH
FGLESMNINFIAASFVRKPQDVLEIRELLEEKNMEDVQIFPKIESQEGIDNTDEILKVSD
HHHHHCCEEEEEHHHHCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHEEECC
GIMIARGDMGVEIPAENVPLVQKTLIKKCNAVGLPVITATQMLDSMIENPRPTRAEASDV
CEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHH
ANAVWDGTDATMLSGESANGDYPVEAVATMAKIDEKAENAMAEDGNLQINTFDQSDVTET
HHHHCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHH
ISAAVARAAKNLGVKTIVAATQSGYTARMISKYRPDADILAITFDERVRRGLMVNWGVHP
HHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHCCCCCCEEEEEEHHHHHCCEEEECCCCE
IIADRPSTTDEMFELAANKAVDLGLAKEGDLILVVAGVPVGESGTTNIMKLQLIGSKLAS
EECCCCCCHHHHHHHHHCCCEEECCCCCCCEEEEEECCCCCCCCCCCEEEEEEEHHHHCC
GQGVGDETVIGKAVVATSADEANKKAVEGGVLVTKTTDKDYLPAIEKSSALVVENGGLTS
CCCCCCCHHHCEEEEEECCCCCCHHHHCCCEEEEECCCCCCCCCCCCCCEEEEECCCCCC
HAAVVGISMGIPVIVGVQNATSILSDDELITVDSRRGIIYHGASNSL
CCEEEEEECCCCEEEECCCHHHHCCCCCEEEEECCCCEEEECCCCCC
>Mature Secondary Structure
MKKTKIVSTLGPASTSVDTIVKLIEAGANVFRFNFSHGDHEEHLGRYNMVKEAEKITGKS
CCCCEEEEECCCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHCCHHHHHHHHHHHCCCE
VGILLDTKGAEIRTTPQKDGNQEYHTNDKVRISMDDTLETTKEKIAVTYDGLFDDVHVGG
EEEEEECCCCEEEECCCCCCCCCCCCCCEEEEEECCHHHHCHHEEEEEECCCCCCEECCC
HVLFDDGLLDFKIDEKDEENRELVAHATNNGVLGSRKGTNAPGVSINLPGITEKDADDIR
EEEEECCEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHH
FGLESMNINFIAASFVRKPQDVLEIRELLEEKNMEDVQIFPKIESQEGIDNTDEILKVSD
HHHHHCCEEEEEHHHHCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHEEECC
GIMIARGDMGVEIPAENVPLVQKTLIKKCNAVGLPVITATQMLDSMIENPRPTRAEASDV
CEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHH
ANAVWDGTDATMLSGESANGDYPVEAVATMAKIDEKAENAMAEDGNLQINTFDQSDVTET
HHHHCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHH
ISAAVARAAKNLGVKTIVAATQSGYTARMISKYRPDADILAITFDERVRRGLMVNWGVHP
HHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHCCCCCCEEEEEEHHHHHCCEEEECCCCE
IIADRPSTTDEMFELAANKAVDLGLAKEGDLILVVAGVPVGESGTTNIMKLQLIGSKLAS
EECCCCCCHHHHHHHHHCCCEEECCCCCCCEEEEEECCCCCCCCCCCEEEEEEEHHHHCC
GQGVGDETVIGKAVVATSADEANKKAVEGGVLVTKTTDKDYLPAIEKSSALVVENGGLTS
CCCCCCCHHHCEEEEEECCCCCCHHHHCCCEEEEECCCCCCCCCCCCCCEEEEECCCCCC
HAAVVGISMGIPVIVGVQNATSILSDDELITVDSRRGIIYHGASNSL
CCEEEEEECCCCEEEECCCHHHHCCCCCEEEEECCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8755908; 8366023; 8274531 [H]