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The map label for this gene is pfkA

Identifier: 116492855

GI number: 116492855

Start: 1088378

End: 1089346

Strand: Reverse

Name: pfkA

Synonym: PEPE_1093

Alternate gene names: 116492855

Gene position: 1089346-1088378 (Counterclockwise)

Preceding gene: 116492856

Following gene: 116492854

Centisome position: 59.45

GC content: 43.24

Gene sequence:

>969_bases
ATGAAACGTATTGGTATCATGACAAGTGGTGGGGATGCTCCAGGGATGAACTTGGCTATCCGCGCCGTTGCAAGAAAGGC
CCTAAGCTCTGGTCTTGAAGCTTACGGAATTAACTACGGGTTTGCCGGTCTAGTTGCTGGTGATATCCACGAATTCAAGG
CAGCAGATTTAGACGATATGGTTTCACAAGGTGGAACGATGCTTTATTCAGCTCGTTATCCTGAATTTGCACAAGAAGAA
AGCCAATTGAAAGGTATTGAACAACTTAAGAAGTTCGGTATCGATGCATTGGTTGTTATCGGAGGAGACGGTTCATATCA
TGGTGCTCTTCGTTTGACAGAACATGGATACAACACCATTGGTCTTCCAGGAACAATCGATAATGATATTCCTTTCACTG
ACTTCACTATTGGTTTTGATACAGCGTTAAACACTGCAGTTGACGCGATTGATAAAATTCGCGATACTGCTAAGAGTCAT
CAACGGGTCTTTGCTGTCCAAGTTATGGGCCGCAATGCTGCTGATATTGCTCTTTGGGCTGGTGTTGCTTCAGGAGCAGA
TGCGGTTATCGCACCTGGTTTTGATTATGACGTTGAAGCAATTGCCAACAAGTTGAAAAAGAACCGTGCAAACGGTAAGG
ACTACGGCATTATTGTCATCGCTGAAGGTGATGCTAATTCAGATGCAGCTCCTGAATTCATTGACCAGCTTAAGCAATAC
GGCGATTTTGATGCACGTGCTACTGTAATTGGACATGTCCAACGTGGTGGTGTTCCAAGTGCTAAAGATCGCGTTTTAGC
AAGTAAGATGGGTGCTTACGCTGTTGAGCTACTCCTTGAAGGTAAGGGTGGTTTAGCAGTTGGAATCTTAGAAAATAAGG
TTCAAGCTCATAACATGCTTGACTTGTTTGATGCAAAACATCAAGCAGATGATTCACTTTACCAATTAAGTGAAGATTTA
TCATTCTAG

Upstream 100 bases:

>100_bases
TCGATTAATTAAGAAAATGGTACAGACAACTGTTTTCAAAAGTGATAAAATCAACAATGAAGTTTTGAAAAAACTCAATA
TTTCTGTTTGAGGTGAAAAG

Downstream 100 bases:

>100_bases
AGTTCTATTAATATTTGGATAAAATGACTTAAGAAGTCTTTTATAATTTAAAATCAAGGGAGAGATTCTGTAATGAAGAA
AACCAAAATCGTTTCAACAC

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MKRIGIMTSGGDAPGMNLAIRAVARKALSSGLEAYGINYGFAGLVAGDIHEFKAADLDDMVSQGGTMLYSARYPEFAQEE
SQLKGIEQLKKFGIDALVVIGGDGSYHGALRLTEHGYNTIGLPGTIDNDIPFTDFTIGFDTALNTAVDAIDKIRDTAKSH
QRVFAVQVMGRNAADIALWAGVASGADAVIAPGFDYDVEAIANKLKKNRANGKDYGIIVIAEGDANSDAAPEFIDQLKQY
GDFDARATVIGHVQRGGVPSAKDRVLASKMGAYAVELLLEGKGGLAVGILENKVQAHNMLDLFDAKHQADDSLYQLSEDL
SF

Sequences:

>Translated_322_residues
MKRIGIMTSGGDAPGMNLAIRAVARKALSSGLEAYGINYGFAGLVAGDIHEFKAADLDDMVSQGGTMLYSARYPEFAQEE
SQLKGIEQLKKFGIDALVVIGGDGSYHGALRLTEHGYNTIGLPGTIDNDIPFTDFTIGFDTALNTAVDAIDKIRDTAKSH
QRVFAVQVMGRNAADIALWAGVASGADAVIAPGFDYDVEAIANKLKKNRANGKDYGIIVIAEGDANSDAAPEFIDQLKQY
GDFDARATVIGHVQRGGVPSAKDRVLASKMGAYAVELLLEGKGGLAVGILENKVQAHNMLDLFDAKHQADDSLYQLSEDL
SF
>Mature_322_residues
MKRIGIMTSGGDAPGMNLAIRAVARKALSSGLEAYGINYGFAGLVAGDIHEFKAADLDDMVSQGGTMLYSARYPEFAQEE
SQLKGIEQLKKFGIDALVVIGGDGSYHGALRLTEHGYNTIGLPGTIDNDIPFTDFTIGFDTALNTAVDAIDKIRDTAKSH
QRVFAVQVMGRNAADIALWAGVASGADAVIAPGFDYDVEAIANKLKKNRANGKDYGIIVIAEGDANSDAAPEFIDQLKQY
GDFDARATVIGHVQRGGVPSAKDRVLASKMGAYAVELLLEGKGGLAVGILENKVQAHNMLDLFDAKHQADDSLYQLSEDL
SF

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family

Homologues:

Organism=Homo sapiens, GI11321601, Length=315, Percent_Identity=40.9523809523809, Blast_Score=202, Evalue=3e-52,
Organism=Homo sapiens, GI266453768, Length=313, Percent_Identity=40.8945686900958, Blast_Score=202, Evalue=5e-52,
Organism=Homo sapiens, GI266453748, Length=313, Percent_Identity=40.8945686900958, Blast_Score=202, Evalue=5e-52,
Organism=Homo sapiens, GI4505749, Length=313, Percent_Identity=40.8945686900958, Blast_Score=202, Evalue=5e-52,
Organism=Homo sapiens, GI266453619, Length=313, Percent_Identity=40.8945686900958, Blast_Score=201, Evalue=6e-52,
Organism=Homo sapiens, GI48762920, Length=311, Percent_Identity=40.8360128617363, Blast_Score=196, Evalue=2e-50,
Organism=Escherichia coli, GI1790350, Length=301, Percent_Identity=52.1594684385382, Blast_Score=305, Evalue=2e-84,
Organism=Caenorhabditis elegans, GI25147584, Length=311, Percent_Identity=38.9067524115756, Blast_Score=195, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17558788, Length=309, Percent_Identity=35.9223300970874, Blast_Score=176, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI72003601, Length=291, Percent_Identity=31.2714776632302, Blast_Score=138, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6323861, Length=342, Percent_Identity=36.8421052631579, Blast_Score=192, Evalue=7e-50,
Organism=Saccharomyces cerevisiae, GI6321679, Length=330, Percent_Identity=38.1818181818182, Blast_Score=186, Evalue=3e-48,
Organism=Drosophila melanogaster, GI24652337, Length=311, Percent_Identity=38.9067524115756, Blast_Score=197, Evalue=1e-50,
Organism=Drosophila melanogaster, GI17647809, Length=311, Percent_Identity=38.9067524115756, Blast_Score=196, Evalue=1e-50,
Organism=Drosophila melanogaster, GI28573326, Length=311, Percent_Identity=39.2282958199357, Blast_Score=196, Evalue=1e-50,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): K6PF_PEDPA (Q03F74)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_804590.1
- ProteinModelPortal:   Q03F74
- SMR:   Q03F74
- STRING:   Q03F74
- GeneID:   4417028
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_1093
- NMPDR:   fig|278197.10.peg.958
- eggNOG:   COG0205
- HOGENOM:   HBG405044
- OMA:   RCEEFKT
- PhylomeDB:   Q03F74
- ProtClustDB:   PRK03202
- BioCyc:   PPEN278197:PEPE_1093-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00339
- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023
- PIRSF:   PIRSF000532
- PRINTS:   PR00476
- TIGRFAMs:   TIGR02482

Pfam domain/function: PF00365 PFK; SSF53784 Ppfruckinase

EC number: =2.7.1.11

Molecular weight: Translated: 34248; Mature: 34248

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: ACT_SITE 127-127 BINDING 162-162 BINDING 246-246 BINDING 252-252 BINDING 255-255

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIGIMTSGGDAPGMNLAIRAVARKALSSGLEAYGINYGFAGLVAGDIHEFKAADLDDM
CCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCHHCCCCCCCHHHHHCCHHHHHCCCHHHH
VSQGGTMLYSARYPEFAQEESQLKGIEQLKKFGIDALVVIGGDGSYHGALRLTEHGYNTI
HHCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCCCCC
GLPGTIDNDIPFTDFTIGFDTALNTAVDAIDKIRDTAKSHQRVFAVQVMGRNAADIALWA
CCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCHHEEEE
GVASGADAVIAPGFDYDVEAIANKLKKNRANGKDYGIIVIAEGDANSDAAPEFIDQLKQY
CCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHH
GDFDARATVIGHVQRGGVPSAKDRVLASKMGAYAVELLLEGKGGLAVGILENKVQAHNML
CCCCCHHHEEEEHHCCCCCCHHHHHHHHHHHHHEEEEEEECCCCEEEEEECCHHHHHHHH
DLFDAKHQADDSLYQLSEDLSF
HHHHHHHCCCHHHHHHHHHCCC
>Mature Secondary Structure
MKRIGIMTSGGDAPGMNLAIRAVARKALSSGLEAYGINYGFAGLVAGDIHEFKAADLDDM
CCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCHHCCCCCCCHHHHHCCHHHHHCCCHHHH
VSQGGTMLYSARYPEFAQEESQLKGIEQLKKFGIDALVVIGGDGSYHGALRLTEHGYNTI
HHCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCCCCC
GLPGTIDNDIPFTDFTIGFDTALNTAVDAIDKIRDTAKSHQRVFAVQVMGRNAADIALWA
CCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCHHEEEE
GVASGADAVIAPGFDYDVEAIANKLKKNRANGKDYGIIVIAEGDANSDAAPEFIDQLKQY
CCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHH
GDFDARATVIGHVQRGGVPSAKDRVLASKMGAYAVELLLEGKGGLAVGILENKVQAHNML
CCCCCHHHEEEEHHCCCCCCHHHHHHHHHHHHHEEEEEEECCCCEEEEEECCHHHHHHHH
DLFDAKHQADDSLYQLSEDLSF
HHHHHHHCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA