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The map label for this gene is qor [C]

Identifier: 116492057

GI number: 116492057

Start: 300239

End: 301174

Strand: Direct

Name: qor [C]

Synonym: PEPE_0253

Alternate gene names: 116492057

Gene position: 300239-301174 (Clockwise)

Preceding gene: 116492056

Following gene: 116492058

Centisome position: 16.39

GC content: 41.99

Gene sequence:

>936_bases
ATGAAAGCAATTGCGATTAGCGCATATGGTGATGTTGACCAACTTCATGAAGAGGAGTTAGATATTCCTCAAATTGCAAA
CGATGAGGTCTTGGTAAAAATTAAAGCAACTAGTATCAATCCGATTGACTGGAAGGCTCGAATGGGATTTTTGAAGCAAA
TGTACGATTGGCAATTCCCAGTTGTTTTAGGATGGGATCTTTCCGGAATAATTACCCAAGTCGGTAAAGATGTTAAGCAT
TTTAAAGTTGGAGATGAAGTCTTTGCTAGACCCGATATTTATGAAGATGGGACGAAAGGGACATATGCAGAATACGCGGC
AGTTAAGGAAGATAAATTAGCATTAAAGCCTAGCAATGTTTCGTTTGAAGCAGCAGCTGCAATTCCATTAGCTGGACTGA
CTGCATGGCAGGTGATTGTAGATCGGTTAAAGATTAAAAAGGGTGATAAAGTTTTAATTCAAGCAGGAGCTGGCGGAGTC
GGGATTTTCGCCATTCAAATTGCTAAACATTTAGGAGCCTATGTAGCCACAACGGCTAGCGCCAAAAATGCAGAATTTTT
AACTAATTTAGGGGCAGATGAAGTCATTGACTACCACGAACACCAAATTGGGGAAGTCCTTAATCAGTATGATGCTGTTT
TTGATACAGTTGATGATTTAGAAGCGGGACTAGCAATTTTGAAACCAACCGGGAAATTGGTCACCATCGCGGGACGCCCA
ACCCAAGCCCAACAGGACGCTCAACCTTCAGCAACTTCTTGGTGGCTCCAACCAAATGGCAAACAACTGGCTGAACTAGG
TGATTTAGTGGCTGAGGGCGAGGTCAAAGTAATTATTGATAGTACATTTCCACTTACTGAAGATGGGTTACGAGCGGCTC
ACGAACGCAGTCAAACTAACCATGCTAGAGGAAAAATTGTTATCAATGTGGAATAG

Upstream 100 bases:

>100_bases
GATTTGGCAATAAATGAGACTTAAATGTAGAACTTACTTTTTATAAGTGATATGCTTAAAAAATATATATTGAATTTATC
TAAGTCTAGGGGGAACAAAA

Downstream 100 bases:

>100_bases
AAAATGAAAATGTAAAAAGTCATGACATTGTTGCTTGTGTCATGATTTTTTTAGTTTAAAATAAAGTTATAGTAACGATG
GAAACGCTTTAACCGAAACT

Product: NADPH:quinone reductase related Zn-dependent oxidoreductase

Products: NADP(+); Semiquinone. [C]

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MKAIAISAYGDVDQLHEEELDIPQIANDEVLVKIKATSINPIDWKARMGFLKQMYDWQFPVVLGWDLSGIITQVGKDVKH
FKVGDEVFARPDIYEDGTKGTYAEYAAVKEDKLALKPSNVSFEAAAAIPLAGLTAWQVIVDRLKIKKGDKVLIQAGAGGV
GIFAIQIAKHLGAYVATTASAKNAEFLTNLGADEVIDYHEHQIGEVLNQYDAVFDTVDDLEAGLAILKPTGKLVTIAGRP
TQAQQDAQPSATSWWLQPNGKQLAELGDLVAEGEVKVIIDSTFPLTEDGLRAAHERSQTNHARGKIVINVE

Sequences:

>Translated_311_residues
MKAIAISAYGDVDQLHEEELDIPQIANDEVLVKIKATSINPIDWKARMGFLKQMYDWQFPVVLGWDLSGIITQVGKDVKH
FKVGDEVFARPDIYEDGTKGTYAEYAAVKEDKLALKPSNVSFEAAAAIPLAGLTAWQVIVDRLKIKKGDKVLIQAGAGGV
GIFAIQIAKHLGAYVATTASAKNAEFLTNLGADEVIDYHEHQIGEVLNQYDAVFDTVDDLEAGLAILKPTGKLVTIAGRP
TQAQQDAQPSATSWWLQPNGKQLAELGDLVAEGEVKVIIDSTFPLTEDGLRAAHERSQTNHARGKIVINVE
>Mature_311_residues
MKAIAISAYGDVDQLHEEELDIPQIANDEVLVKIKATSINPIDWKARMGFLKQMYDWQFPVVLGWDLSGIITQVGKDVKH
FKVGDEVFARPDIYEDGTKGTYAEYAAVKEDKLALKPSNVSFEAAAAIPLAGLTAWQVIVDRLKIKKGDKVLIQAGAGGV
GIFAIQIAKHLGAYVATTASAKNAEFLTNLGADEVIDYHEHQIGEVLNQYDAVFDTVDDLEAGLAILKPTGKLVTIAGRP
TQAQQDAQPSATSWWLQPNGKQLAELGDLVAEGEVKVIIDSTFPLTEDGLRAAHERSQTNHARGKIVINVE

Specific function: Unknown

COG id: COG0604

COG function: function code CR; NADPH:quinone reductase and related Zn-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI47519420, Length=358, Percent_Identity=31.2849162011173, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI194239674, Length=252, Percent_Identity=27.7777777777778, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI13236495, Length=252, Percent_Identity=27.7777777777778, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI194239676, Length=202, Percent_Identity=29.7029702970297, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI18379349, Length=269, Percent_Identity=27.8810408921933, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI24308257, Length=264, Percent_Identity=25.7575757575758, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI22538446, Length=209, Percent_Identity=32.0574162679426, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI22538444, Length=209, Percent_Identity=32.0574162679426, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI67078404, Length=219, Percent_Identity=28.310502283105, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI90669511, Length=167, Percent_Identity=31.7365269461078, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1790485, Length=242, Percent_Identity=33.8842975206612, Blast_Score=107, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17507255, Length=331, Percent_Identity=29.3051359516616, Blast_Score=113, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71987554, Length=353, Percent_Identity=28.8951841359773, Blast_Score=103, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI71988145, Length=219, Percent_Identity=26.4840182648402, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17536829, Length=178, Percent_Identity=31.4606741573034, Blast_Score=64, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6319520, Length=347, Percent_Identity=27.9538904899135, Blast_Score=95, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6324195, Length=258, Percent_Identity=26.7441860465116, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319945, Length=252, Percent_Identity=28.1746031746032, Blast_Score=70, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6323493, Length=252, Percent_Identity=28.1746031746032, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24581341, Length=367, Percent_Identity=28.8828337874659, Blast_Score=104, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24581339, Length=367, Percent_Identity=28.8828337874659, Blast_Score=104, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR014182
- InterPro:   IPR011032
- InterPro:   IPR016040
- InterPro:   IPR002364 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 33814; Mature: 33814

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS01162 QOR_ZETA_CRYSTAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAIAISAYGDVDQLHEEELDIPQIANDEVLVKIKATSINPIDWKARMGFLKQMYDWQFP
CCEEEEECCCCHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCC
VVLGWDLSGIITQVGKDVKHFKVGDEVFARPDIYEDGTKGTYAEYAAVKEDKLALKPSNV
EEECCCHHHHHHHHCCCHHHEECCCHHHCCCCCCCCCCCCCHHHHHHCCCCCEEECCCCC
SFEAAAAIPLAGLTAWQVIVDRLKIKKGDKVLIQAGAGGVGIFAIQIAKHLGAYVATTAS
CEEHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCHHEEECCC
AKNAEFLTNLGADEVIDYHEHQIGEVLNQYDAVFDTVDDLEAGLAILKPTGKLVTIAGRP
CCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEEEECCC
TQAQQDAQPSATSWWLQPNGKQLAELGDLVAEGEVKVIIDSTFPLTEDGLRAAHERSQTN
CCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHCCC
HARGKIVINVE
CCCCEEEEEEC
>Mature Secondary Structure
MKAIAISAYGDVDQLHEEELDIPQIANDEVLVKIKATSINPIDWKARMGFLKQMYDWQFP
CCEEEEECCCCHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCC
VVLGWDLSGIITQVGKDVKHFKVGDEVFARPDIYEDGTKGTYAEYAAVKEDKLALKPSNV
EEECCCHHHHHHHHCCCHHHEECCCHHHCCCCCCCCCCCCCHHHHHHCCCCCEEECCCCC
SFEAAAAIPLAGLTAWQVIVDRLKIKKGDKVLIQAGAGGVGIFAIQIAKHLGAYVATTAS
CEEHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCHHEEECCC
AKNAEFLTNLGADEVIDYHEHQIGEVLNQYDAVFDTVDDLEAGLAILKPTGKLVTIAGRP
CCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCEEEEECCC
TQAQQDAQPSATSWWLQPNGKQLAELGDLVAEGEVKVIIDSTFPLTEDGLRAAHERSQTN
CCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHCCC
HARGKIVINVE
CCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.065 {1,4-benzoquinone}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.26 {1,4-naphthoquinone}} 0.0024 {1,2-naphthoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA