Click here to switch to the map view.

The map label for this gene is tyrS

Identifier: 116492043

GI number: 116492043

Start: 276150

End: 277403

Strand: Direct

Name: tyrS

Synonym: PEPE_0235

Alternate gene names: 116492043

Gene position: 276150-277403 (Clockwise)

Preceding gene: 116492042

Following gene: 116492044

Centisome position: 15.07

GC content: 36.92

Gene sequence:

>1254_bases
ATGAATATTATTGATGAACTTACTTGGCGTGGTGCGGTTAACCAACAAACAGACGAAGAAGGCTTGAAAAAGTTAACAGA
CGAAAAGAAGATTGGGCTTTATGCTGGTATTGATCCAACGGGTGATAGTATGCATATTGGACACTTAATTCCTTTCATGG
TTTTGAAGAGATTCCAACAAGCAGGTCATAAACCAGTTATTTTAATTGGTGGTGGGACTGGATCGATTGGTGATCCATCA
GGTAAAAAATCAGAACGAGTTCTACAAACTATGGAACAAGTTCACCATAATGAAGAAGCCTTGAAGTCTCAAATGGTGAA
ATTTTTTGGAACCGATAATTTCCGAATGGTTAACAATTATGATTGGCTTTCTAAAATGTCCTTGTTAGACTTCTTACGTG
ATTACGGTAAGTTATTTAACGTTAATACGATGTTAGCTAAGGATATCGTAGCAAGTCGTTTGGAAGTTGGTATTTCATTT
ACAGAATTTACATACCAAATTCTACAATCAATTGACTTTTTACATCTATATAAGAATGAAGATGTTCAACTTCAAATCGG
TGGAGGAGATCAATGGGGTAATATTACAGCTGGTACAGATTTAATTCACCGAATGGAAGGTCAAGAAGCAAAGGTTTATG
GTTTAACTATTCCATTGTTATTGAAAGCTGACGGAACCAAGTTTGGTAAGTCCGAGGGTGGTAACGTTTGGCTAGATGCT
GAAAAGACCACTCCTTATGAATTCTACCAATTCTGGTTGAATCAAGATGATCGGGATGTAGTTAAATTCTTGAAGTACTT
TACATTCTTAAGTCATGAAGAAATTGAACGTTTGGCTGAAACAGTTAAAACAGCTCCAGAAAAACGAGAAGCACAACGTC
GTTTGGCAGAAGAAGTTACTTCATTTGTGCACGGGGATGCAGCGGTTGAAGAAGCACAACATATTTCAGCAGCTTTATTC
TCAGGTGAAGTAAAAGATTTAACTGCTAGTGAAATTGAACAAGGCTTTAAGAATATGCCAAGTGTTGATGTTGAGAATAA
GAAAGAAAACATTGTACTTTGGTTAGTTGATACCACTAAGATTGAATCATCACGGCGCCAAGCTCGGGAAGACATTCAAA
ATGGAGCTATTCGAATTAACGGAGAAAAAGTAACAGACGTTAATGCTGAAATCGACCCTGCAAGTAACTTTGATGGTAAG
TTTGTAATTGTACGTCGGGGAAAGAAGAAGTATTTCTTAGCTCGAGTAAAATAA

Upstream 100 bases:

>100_bases
GGTGGTAACGCGGAAAAATTCGTCCCTGATCTTAATTTTTTAAGATTGGGGACGTTTTTATTTCTAATTGAAGATGGTCG
ATAAAATTGGAGGAATGAAA

Downstream 100 bases:

>100_bases
TAGTATAAAAAAGATTGAGTCAATTTTGGCTCAATCTTTTTTATTTTCAATATATATGGGCTTAAGAGCGATGAAAATAA
CACCCCCAAGGGATGTGAAT

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MNIIDELTWRGAVNQQTDEEGLKKLTDEKKIGLYAGIDPTGDSMHIGHLIPFMVLKRFQQAGHKPVILIGGGTGSIGDPS
GKKSERVLQTMEQVHHNEEALKSQMVKFFGTDNFRMVNNYDWLSKMSLLDFLRDYGKLFNVNTMLAKDIVASRLEVGISF
TEFTYQILQSIDFLHLYKNEDVQLQIGGGDQWGNITAGTDLIHRMEGQEAKVYGLTIPLLLKADGTKFGKSEGGNVWLDA
EKTTPYEFYQFWLNQDDRDVVKFLKYFTFLSHEEIERLAETVKTAPEKREAQRRLAEEVTSFVHGDAAVEEAQHISAALF
SGEVKDLTASEIEQGFKNMPSVDVENKKENIVLWLVDTTKIESSRRQAREDIQNGAIRINGEKVTDVNAEIDPASNFDGK
FVIVRRGKKKYFLARVK

Sequences:

>Translated_417_residues
MNIIDELTWRGAVNQQTDEEGLKKLTDEKKIGLYAGIDPTGDSMHIGHLIPFMVLKRFQQAGHKPVILIGGGTGSIGDPS
GKKSERVLQTMEQVHHNEEALKSQMVKFFGTDNFRMVNNYDWLSKMSLLDFLRDYGKLFNVNTMLAKDIVASRLEVGISF
TEFTYQILQSIDFLHLYKNEDVQLQIGGGDQWGNITAGTDLIHRMEGQEAKVYGLTIPLLLKADGTKFGKSEGGNVWLDA
EKTTPYEFYQFWLNQDDRDVVKFLKYFTFLSHEEIERLAETVKTAPEKREAQRRLAEEVTSFVHGDAAVEEAQHISAALF
SGEVKDLTASEIEQGFKNMPSVDVENKKENIVLWLVDTTKIESSRRQAREDIQNGAIRINGEKVTDVNAEIDPASNFDGK
FVIVRRGKKKYFLARVK
>Mature_417_residues
MNIIDELTWRGAVNQQTDEEGLKKLTDEKKIGLYAGIDPTGDSMHIGHLIPFMVLKRFQQAGHKPVILIGGGTGSIGDPS
GKKSERVLQTMEQVHHNEEALKSQMVKFFGTDNFRMVNNYDWLSKMSLLDFLRDYGKLFNVNTMLAKDIVASRLEVGISF
TEFTYQILQSIDFLHLYKNEDVQLQIGGGDQWGNITAGTDLIHRMEGQEAKVYGLTIPLLLKADGTKFGKSEGGNVWLDA
EKTTPYEFYQFWLNQDDRDVVKFLKYFTFLSHEEIERLAETVKTAPEKREAQRRLAEEVTSFVHGDAAVEEAQHISAALF
SGEVKDLTASEIEQGFKNMPSVDVENKKENIVLWLVDTTKIESSRRQAREDIQNGAIRINGEKVTDVNAEIDPASNFDGK
FVIVRRGKKKYFLARVK

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=375, Percent_Identity=37.0666666666667, Blast_Score=229, Evalue=4e-60,
Organism=Escherichia coli, GI1787925, Length=420, Percent_Identity=49.2857142857143, Blast_Score=393, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI25145802, Length=395, Percent_Identity=38.2278481012658, Blast_Score=237, Evalue=8e-63,
Organism=Caenorhabditis elegans, GI25145805, Length=332, Percent_Identity=35.2409638554217, Blast_Score=172, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6325160, Length=441, Percent_Identity=38.0952380952381, Blast_Score=301, Evalue=9e-83,
Organism=Drosophila melanogaster, GI20130383, Length=434, Percent_Identity=36.8663594470046, Blast_Score=287, Evalue=1e-77,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_PEDPA (Q03HI6)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_803778.1
- ProteinModelPortal:   Q03HI6
- SMR:   Q03HI6
- STRING:   Q03HI6
- GeneID:   4418578
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_0235
- NMPDR:   fig|278197.10.peg.218
- eggNOG:   COG0162
- HOGENOM:   HBG288125
- OMA:   WINQDDR
- PhylomeDB:   Q03HI6
- ProtClustDB:   PRK13354
- BioCyc:   PPEN278197:PEPE_0235-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_02006
- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- SMART:   SM00363
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 47249; Mature: 47249

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 34-34 BINDING 165-165 BINDING 169-169 BINDING 230-230

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIIDELTWRGAVNQQTDEEGLKKLTDEKKIGLYAGIDPTGDSMHIGHLIPFMVLKRFQQ
CCCHHHHHHCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCCCCEEHHHHHHHHHHHHHHH
AGHKPVILIGGGTGSIGDPSGKKSERVLQTMEQVHHNEEALKSQMVKFFGTDNFRMVNNY
CCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEECCC
DWLSKMSLLDFLRDYGKLFNVNTMLAKDIVASRLEVGISFTEFTYQILQSIDFLHLYKNE
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEEECCC
DVQLQIGGGDQWGNITAGTDLIHRMEGQEAKVYGLTIPLLLKADGTKFGKSEGGNVWLDA
CEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEEEEEEEEEEECCCCCCCCCCCCEEEEC
EKTTPYEFYQFWLNQDDRDVVKFLKYFTFLSHEEIERLAETVKTAPEKREAQRRLAEEVT
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
SFVHGDAAVEEAQHISAALFSGEVKDLTASEIEQGFKNMPSVDVENKKENIVLWLVDTTK
HHHCCHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEEECHH
IESSRRQAREDIQNGAIRINGEKVTDVNAEIDPASNFDGKFVIVRRGKKKYFLARVK
HHHHHHHHHHHHHCCCEEECCCEEEECCCCCCCCCCCCCEEEEEECCCCEEEEEECC
>Mature Secondary Structure
MNIIDELTWRGAVNQQTDEEGLKKLTDEKKIGLYAGIDPTGDSMHIGHLIPFMVLKRFQQ
CCCHHHHHHCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCCCCEEHHHHHHHHHHHHHHH
AGHKPVILIGGGTGSIGDPSGKKSERVLQTMEQVHHNEEALKSQMVKFFGTDNFRMVNNY
CCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEECCC
DWLSKMSLLDFLRDYGKLFNVNTMLAKDIVASRLEVGISFTEFTYQILQSIDFLHLYKNE
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEEECCC
DVQLQIGGGDQWGNITAGTDLIHRMEGQEAKVYGLTIPLLLKADGTKFGKSEGGNVWLDA
CEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEEEEEEEEEEECCCCCCCCCCCCEEEEC
EKTTPYEFYQFWLNQDDRDVVKFLKYFTFLSHEEIERLAETVKTAPEKREAQRRLAEEVT
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
SFVHGDAAVEEAQHISAALFSGEVKDLTASEIEQGFKNMPSVDVENKKENIVLWLVDTTK
HHHCCHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEEECHH
IESSRRQAREDIQNGAIRINGEKVTDVNAEIDPASNFDGKFVIVRRGKKKYFLARVK
HHHHHHHHHHHHHCCCEEECCCEEEECCCCCCCCCCCCCEEEEEECCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA