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The map label for this gene is pucI [H]

Identifier: 116492044

GI number: 116492044

Start: 283174

End: 284538

Strand: Direct

Name: pucI [H]

Synonym: PEPE_0240

Alternate gene names: 116492044

Gene position: 283174-284538 (Clockwise)

Preceding gene: 116492043

Following gene: 116492045

Centisome position: 15.45

GC content: 37.07

Gene sequence:

>1365_bases
ATGAATACAAAGTACCAAGTAGAAAGTATTGATAAGAAACATCGACATATGACATATTGGGATATGTTTGCAACCTGGGT
TGGTGCTAATGCTAACAATGGAACCTGGTTCATCGGTGGAATTATTGCTGCTTGTGGATTCTGGGGCGGAATTAAAGCCT
TAATTTTAGCTTCGAGTATTTCATACATATTCTTAATGTTAGTTGGTTATATGGGCACCAAAACTGGAGTTACAACAATG
GCGTTGTCACGTGCTTCTTTTGGAATTAGAGGCAGCATTATTCCTTCGATTGTTAATTTGACACAATTTGTTGGTTGGAC
AGCGGTTAATACTTTTATTGCTGCGACTTCTGTATCATATATTTTGCACAGCTTCTTGGGATGGCCTGTATATGGTCAAC
CGGGTGGAAATCTGGGATTGATTATGGGAATTGCAGTGATGAGTATCCTACATTTAATTAGTATTGTTTCAGGACAGAAA
TCCATTCAAATTATTGAACGAATTGGGATTATCTTAGTTATTATCTTTGTGATTTGGGAATCAATAGTAGTTTTTAAAGA
TGTATCCTTTAACCAAATCGCTGCTTGGAAAGTTCCAAGCCATCTTAAAATGGGGTTTGGGACGGCCATGGACACGTTAG
CCGCCTTTAATTTAGCGTGGGTGACTGCTGGCGCGGATTTTACTAGATTTACTAAGAAATCTGTTACAGCGACCACCGCA
CCTTTTCTAGGAGCAAACTTAGGTGTTTTCGGATTTGCATTTATTGGATTAAGTGCCACAATAAGTATTGCAATTAGTTC
AGGCGTGTACGATCCTAATAATTCTGATCCAAGTACAATTGCTAATAGATTAGGGTTAGGGCTCATTGCAATGATTGTAA
TTATGCTGACTAGCATGACGGCCAATGCCGTCAATATTCAGGCAGCGGGTTCAGCATTAAACAATATGTTCACTAAGCTA
ACTTTAAAACAGTCACTACTCATTACCACGGTTATTGCAACCTTAGTAACTTTTATACCGGTTTTCTATGGTAGTTTTTT
AGAAACCTTCACCGCTTTCTTGGACTATGTAGGTATGGTTCTAGGACCGATTATTAGTATTATGGTGGTTGATTATTTCC
TTATTTCAAAACAGAAATATGATCCTATGGAATTGGGAAATCCAAACGGAAGATACTGGTATAAAAATGGTATCCACTGG
ACGGCTTTTATTTTATGGTTAGTAGGCGTAATTTTTTACAGCTTATTACAGAAATTGGACTTTGTTCAAACCTATACTGG
GGCAACGTTCATTGTAATGATTATTATTGGAATTTTATATTGGCTAATTATGCAGTTTGAAAAGAGGAACAGCAATGTTA
ATTAA

Upstream 100 bases:

>100_bases
TTTTGAAAGTTAAATTTGAACCAATTCATCTTTGATTGTGTACAGTGAATCTAGTATAATTTTTTAAAATGTGTAAAAAA
TACTAGGAGGAACTGTGCGA

Downstream 100 bases:

>100_bases
AAACGTTCATATCGAAAATGATCCTGAAGTAAAAGATGTTAGAATCGAAAATGGAATTTTTAAAGCAATTGGTAAAAATC
TTGAAGCATCTGATGACGAG

Product: purine-cytosine permease related protein

Products: NA

Alternate protein names: Allantoin transport protein [H]

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MNTKYQVESIDKKHRHMTYWDMFATWVGANANNGTWFIGGIIAACGFWGGIKALILASSISYIFLMLVGYMGTKTGVTTM
ALSRASFGIRGSIIPSIVNLTQFVGWTAVNTFIAATSVSYILHSFLGWPVYGQPGGNLGLIMGIAVMSILHLISIVSGQK
SIQIIERIGIILVIIFVIWESIVVFKDVSFNQIAAWKVPSHLKMGFGTAMDTLAAFNLAWVTAGADFTRFTKKSVTATTA
PFLGANLGVFGFAFIGLSATISIAISSGVYDPNNSDPSTIANRLGLGLIAMIVIMLTSMTANAVNIQAAGSALNNMFTKL
TLKQSLLITTVIATLVTFIPVFYGSFLETFTAFLDYVGMVLGPIISIMVVDYFLISKQKYDPMELGNPNGRYWYKNGIHW
TAFILWLVGVIFYSLLQKLDFVQTYTGATFIVMIIIGILYWLIMQFEKRNSNVN

Sequences:

>Translated_454_residues
MNTKYQVESIDKKHRHMTYWDMFATWVGANANNGTWFIGGIIAACGFWGGIKALILASSISYIFLMLVGYMGTKTGVTTM
ALSRASFGIRGSIIPSIVNLTQFVGWTAVNTFIAATSVSYILHSFLGWPVYGQPGGNLGLIMGIAVMSILHLISIVSGQK
SIQIIERIGIILVIIFVIWESIVVFKDVSFNQIAAWKVPSHLKMGFGTAMDTLAAFNLAWVTAGADFTRFTKKSVTATTA
PFLGANLGVFGFAFIGLSATISIAISSGVYDPNNSDPSTIANRLGLGLIAMIVIMLTSMTANAVNIQAAGSALNNMFTKL
TLKQSLLITTVIATLVTFIPVFYGSFLETFTAFLDYVGMVLGPIISIMVVDYFLISKQKYDPMELGNPNGRYWYKNGIHW
TAFILWLVGVIFYSLLQKLDFVQTYTGATFIVMIIIGILYWLIMQFEKRNSNVN
>Mature_454_residues
MNTKYQVESIDKKHRHMTYWDMFATWVGANANNGTWFIGGIIAACGFWGGIKALILASSISYIFLMLVGYMGTKTGVTTM
ALSRASFGIRGSIIPSIVNLTQFVGWTAVNTFIAATSVSYILHSFLGWPVYGQPGGNLGLIMGIAVMSILHLISIVSGQK
SIQIIERIGIILVIIFVIWESIVVFKDVSFNQIAAWKVPSHLKMGFGTAMDTLAAFNLAWVTAGADFTRFTKKSVTATTA
PFLGANLGVFGFAFIGLSATISIAISSGVYDPNNSDPSTIANRLGLGLIAMIVIMLTSMTANAVNIQAAGSALNNMFTKL
TLKQSLLITTVIATLVTFIPVFYGSFLETFTAFLDYVGMVLGPIISIMVVDYFLISKQKYDPMELGNPNGRYWYKNGIHW
TAFILWLVGVIFYSLLQKLDFVQTYTGATFIVMIIIGILYWLIMQFEKRNSNVN

Specific function: Transport of allantoin [H]

COG id: COG1457

COG function: function code F; Purine-cytosine permease and related proteins

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine-cytosine permease family [H]

Homologues:

Organism=Escherichia coli, GI87081749, Length=472, Percent_Identity=24.364406779661, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001248
- InterPro:   IPR012681 [H]

Pfam domain/function: PF02133 Transp_cyt_pur [H]

EC number: NA

Molecular weight: Translated: 49745; Mature: 49745

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure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HHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 11344136 [H]