Click here to switch to the map view.

The map label for this gene is cysS

Identifier: 116515227

GI number: 116515227

Start: 347242

End: 348672

Strand: Reverse

Name: cysS

Synonym: BCc_306

Alternate gene names: 116515227

Gene position: 348672-347242 (Counterclockwise)

Preceding gene: 116515229

Following gene: 116515215

Centisome position: 83.74

GC content: 19.78

Gene sequence:

>1431_bases
ATGTTAAGAATTTTTAATACATTAACAAAAAAAAAAGAAATTTTTGATTTTTTATTGAATAAGAAAATTAATATTTATGT
TTGTGGAGTCACTACATATGATTTTTGTCATATAGGACACGCTCGTACATTTATTATATTTGATATTATCATACGTTATT
TACAATATTTAGGATATAATACTTTTTATATAAGAAATATTACTGATATTGATGATAAAATTATTAATAAAGCAAAAATA
AATAATGAATCAATTCATATTCTAGTTAATCGTATGATTAAATTAATGCATAAAGATTTTTTATCTTTAAATTTAATTAA
ACCTGATCAAGAACCTAGAGTTACACAACACATATCTAATATTATTTCTTCTATTAAGTTATTATTAAAAAATAATTATG
CTTATGTTTCTGATAATAAAGATATTTTATTTAAACTTAATAATTTTAAGGAATATGGATTTTTATCTAATAGAATGTTT
AATTGTTCAGAAAAAAATTTTACTACTATTAGTAAAGAAAAAAATTATATTAATAACATTAATGATTTTGTTCTTTGGAA
ACATAGTAATCAATTATCAATAGATACATGTACAAATTGGTCTTCCCCTTGGGGAGCTGGTCGTCCAGGATGGCATATTG
AATGTTCTTCTATAATTAATAATTTTTTTAAAGATGGAGTAGTAGATATTCATGGAGGAGGGATTGATTTATTATTTCCT
CATCATGAAAATGAAATAGCACAATTAAAAAGTATGAATAATTCTTTTTCTGTTAATTTTTGGATACATTCAGGTATGGT
TATTAATAAAAATCATAAAATGTCAAAATCATTTTCTAATTCTGTTTCTATTCAATATTTATTGAAAAAATATGATTCTG
AAATTATTAGATGGTATTTTCTTGCTACTCATTATAGACACCCACTTTATTATTCTGAAAAAAATTTATTAATGATGAAA
AATATTTTTATTAAGATTTATAGATCTTTATTAGATTGTGTTGTAACTATTAATACAAAAATTGATTATGAATATCATTT
AAGAAATAAAATAAAAAGAAAATTTTTTAGTGCAATGAATGATGATTTTAATACACCCAAAGCATGTAGTATTTTGCAAA
AAATATCAAAATTAATTTACAAAAATAAAAAAAATAATACTTATTTAGCTAATATTTTAGCATCTGATTTAGTTTATTTA
GGAAAAATTTTAGGTTTATTTCAAAATGATCCTAAAAATTTTTTTTTAAAAGATAATTTTTGTAATAAATCAACTGATTT
ATTAATTATAGTTGAAAAATTGTTTCATATGAGAAATTTTTATAGATCTAAAAAACAATGGAATTTATCAGACATTATTA
GAAAAAAATTGTTTTGTTTAGGTGTTATTGTAGAAGATAATTCTTATTCTTCATATTATAGATTTATATAA

Upstream 100 bases:

>100_bases
AAAATTAAATTTTTTTAAATATTTTTAAATTTCTACTGTTATAAAATTTTTTAATAATTTTAAATACTTAAAATAATAAA
TAATTGTTAAGAGAATTTAT

Downstream 100 bases:

>100_bases
TTAAATATTTTTACATTATACACGCTTGTAGTGTATTTTCTAATAATGATACTACGGTCATAGGACCTACTCCTCCTGGT
ACTGGAGTAATATAAGACGT

Product: hypothetical protein

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MLRIFNTLTKKKEIFDFLLNKKINIYVCGVTTYDFCHIGHARTFIIFDIIIRYLQYLGYNTFYIRNITDIDDKIINKAKI
NNESIHILVNRMIKLMHKDFLSLNLIKPDQEPRVTQHISNIISSIKLLLKNNYAYVSDNKDILFKLNNFKEYGFLSNRMF
NCSEKNFTTISKEKNYINNINDFVLWKHSNQLSIDTCTNWSSPWGAGRPGWHIECSSIINNFFKDGVVDIHGGGIDLLFP
HHENEIAQLKSMNNSFSVNFWIHSGMVINKNHKMSKSFSNSVSIQYLLKKYDSEIIRWYFLATHYRHPLYYSEKNLLMMK
NIFIKIYRSLLDCVVTINTKIDYEYHLRNKIKRKFFSAMNDDFNTPKACSILQKISKLIYKNKKNNTYLANILASDLVYL
GKILGLFQNDPKNFFLKDNFCNKSTDLLIIVEKLFHMRNFYRSKKQWNLSDIIRKKLFCLGVIVEDNSYSSYYRFI

Sequences:

>Translated_476_residues
MLRIFNTLTKKKEIFDFLLNKKINIYVCGVTTYDFCHIGHARTFIIFDIIIRYLQYLGYNTFYIRNITDIDDKIINKAKI
NNESIHILVNRMIKLMHKDFLSLNLIKPDQEPRVTQHISNIISSIKLLLKNNYAYVSDNKDILFKLNNFKEYGFLSNRMF
NCSEKNFTTISKEKNYINNINDFVLWKHSNQLSIDTCTNWSSPWGAGRPGWHIECSSIINNFFKDGVVDIHGGGIDLLFP
HHENEIAQLKSMNNSFSVNFWIHSGMVINKNHKMSKSFSNSVSIQYLLKKYDSEIIRWYFLATHYRHPLYYSEKNLLMMK
NIFIKIYRSLLDCVVTINTKIDYEYHLRNKIKRKFFSAMNDDFNTPKACSILQKISKLIYKNKKNNTYLANILASDLVYL
GKILGLFQNDPKNFFLKDNFCNKSTDLLIIVEKLFHMRNFYRSKKQWNLSDIIRKKLFCLGVIVEDNSYSSYYRFI
>Mature_476_residues
MLRIFNTLTKKKEIFDFLLNKKINIYVCGVTTYDFCHIGHARTFIIFDIIIRYLQYLGYNTFYIRNITDIDDKIINKAKI
NNESIHILVNRMIKLMHKDFLSLNLIKPDQEPRVTQHISNIISSIKLLLKNNYAYVSDNKDILFKLNNFKEYGFLSNRMF
NCSEKNFTTISKEKNYINNINDFVLWKHSNQLSIDTCTNWSSPWGAGRPGWHIECSSIINNFFKDGVVDIHGGGIDLLFP
HHENEIAQLKSMNNSFSVNFWIHSGMVINKNHKMSKSFSNSVSIQYLLKKYDSEIIRWYFLATHYRHPLYYSEKNLLMMK
NIFIKIYRSLLDCVVTINTKIDYEYHLRNKIKRKFFSAMNDDFNTPKACSILQKISKLIYKNKKNNTYLANILASDLVYL
GKILGLFQNDPKNFFLKDNFCNKSTDLLIIVEKLFHMRNFYRSKKQWNLSDIIRKKLFCLGVIVEDNSYSSYYRFI

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=378, Percent_Identity=32.8042328042328, Blast_Score=199, Evalue=4e-51,
Organism=Homo sapiens, GI303304987, Length=418, Percent_Identity=27.7511961722488, Blast_Score=142, Evalue=5e-34,
Organism=Homo sapiens, GI21269877, Length=419, Percent_Identity=27.6849642004773, Blast_Score=142, Evalue=5e-34,
Organism=Homo sapiens, GI62240992, Length=419, Percent_Identity=27.6849642004773, Blast_Score=142, Evalue=6e-34,
Organism=Homo sapiens, GI10835051, Length=419, Percent_Identity=27.6849642004773, Blast_Score=142, Evalue=6e-34,
Organism=Escherichia coli, GI1786737, Length=480, Percent_Identity=41.25, Blast_Score=389, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17509795, Length=252, Percent_Identity=31.3492063492063, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17509797, Length=252, Percent_Identity=31.3492063492063, Blast_Score=133, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6324082, Length=325, Percent_Identity=30.7692307692308, Blast_Score=146, Evalue=9e-36,
Organism=Drosophila melanogaster, GI19922218, Length=488, Percent_Identity=30.9426229508197, Blast_Score=214, Evalue=1e-55,
Organism=Drosophila melanogaster, GI19922364, Length=257, Percent_Identity=33.4630350194552, Blast_Score=149, Evalue=4e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_BUCCC (Q057D6)

Other databases:

- EMBL:   CP000263
- RefSeq:   YP_802856.1
- ProteinModelPortal:   Q057D6
- SMR:   Q057D6
- STRING:   Q057D6
- EnsemblBacteria:   EBBUCT00000001491
- GeneID:   4440913
- GenomeReviews:   CP000263_GR
- KEGG:   bcc:BCc_306
- eggNOG:   COG0215
- GeneTree:   EBGT00050000008180
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- BioCyc:   BAPH372461:BCC_306-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 56421; Mature: 56421

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 276-276

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRIFNTLTKKKEIFDFLLNKKINIYVCGVTTYDFCHIGHARTFIIFDIIIRYLQYLGYN
CCCHHHHHHHHHHHHHHHHCCCEEEEEEECCEEHHHCCCCHHHHHHHHHHHHHHHHHCCC
TFYIRNITDIDDKIINKAKINNESIHILVNRMIKLMHKDFLSLNLIKPDQEPRVTQHISN
EEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHH
IISSIKLLLKNNYAYVSDNKDILFKLNNFKEYGFLSNRMFNCSEKNFTTISKEKNYINNI
HHHHHHHHHHCCEEEEECCCEEEEEECCCCCCCCHHCCEECCCCCCCEEEHHCHHHHCCC
NDFVLWKHSNQLSIDTCTNWSSPWGAGRPGWHIECSSIINNFFKDGVVDIHGGGIDLLFP
CCEEEEECCCEEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCEEEECCCEEEEEC
HHENEIAQLKSMNNSFSVNFWIHSGMVINKNHKMSKSFSNSVSIQYLLKKYDSEIIRWYF
CCCCHHHHHHHCCCCEEEEEEEECCEEEECCCCCCHHHCCCHHHHHHHHHHHHHHHHHHH
LATHYRHPLYYSEKNLLMMKNIFIKIYRSLLDCVVTINTKIDYEYHLRNKIKRKFFSAMN
HHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHEEEECCEECEEHHHHHHHHHHHHHHHC
DDFNTPKACSILQKISKLIYKNKKNNTYLANILASDLVYLGKILGLFQNDPKNFFLKDNF
CCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCC
CNKSTDLLIIVEKLFHMRNFYRSKKQWNLSDIIRKKLFCLGVIVEDNSYSSYYRFI
CCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MLRIFNTLTKKKEIFDFLLNKKINIYVCGVTTYDFCHIGHARTFIIFDIIIRYLQYLGYN
CCCHHHHHHHHHHHHHHHHCCCEEEEEEECCEEHHHCCCCHHHHHHHHHHHHHHHHHCCC
TFYIRNITDIDDKIINKAKINNESIHILVNRMIKLMHKDFLSLNLIKPDQEPRVTQHISN
EEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHH
IISSIKLLLKNNYAYVSDNKDILFKLNNFKEYGFLSNRMFNCSEKNFTTISKEKNYINNI
HHHHHHHHHHCCEEEEECCCEEEEEECCCCCCCCHHCCEECCCCCCCEEEHHCHHHHCCC
NDFVLWKHSNQLSIDTCTNWSSPWGAGRPGWHIECSSIINNFFKDGVVDIHGGGIDLLFP
CCEEEEECCCEEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCEEEECCCEEEEEC
HHENEIAQLKSMNNSFSVNFWIHSGMVINKNHKMSKSFSNSVSIQYLLKKYDSEIIRWYF
CCCCHHHHHHHCCCCEEEEEEEECCEEEECCCCCCHHHCCCHHHHHHHHHHHHHHHHHHH
LATHYRHPLYYSEKNLLMMKNIFIKIYRSLLDCVVTINTKIDYEYHLRNKIKRKFFSAMN
HHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHEEEECCEECEEHHHHHHHHHHHHHHHC
DDFNTPKACSILQKISKLIYKNKKNNTYLANILASDLVYLGKILGLFQNDPKNFFLKDNF
CCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCC
CNKSTDLLIIVEKLFHMRNFYRSKKQWNLSDIIRKKLFCLGVIVEDNSYSSYYRFI
CCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA