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The map label for this gene is lipB [H]

Identifier: 116515105

GI number: 116515105

Start: 197331

End: 197939

Strand: Direct

Name: lipB [H]

Synonym: BCc_169

Alternate gene names: 116515105

Gene position: 197331-197939 (Clockwise)

Preceding gene: 116515104

Following gene: 116515106

Centisome position: 47.39

GC content: 19.21

Gene sequence:

>609_bases
ATGAATATTATAATTCGAAATTTAGGTATATGTAACTGGTTAGGTATAGAAGAAAAAATGATAAAATTTACTAAGAATAG
AAGCTTTAATACTTCTGATGAATTATGGTGTTTAGAACATTATCCAGTTTTTACTTATGGAGTATTAGAAAAAAAAAAAT
ATGCACCATTTATACATAAAATACCAGTATTTAAATCAAATAGAGGAGGAAAAATTACATTTCATGGACCAGGACAATTA
ATTATTTATTTATTAATTGATTTGCGAAGAAAAAAAATTAAATTTTATGAATTAGTAAATAGAATAGAAATATTAATTAT
ACATATATTAAAAATTTTAAAAATATCATCCTATACAAAAAAAAAACATCCTGGAATTTATGTTAAAAAAAAAAAAATTT
GTTCGTTAGGTTTTCGAATAATTAATGGTTGTTCACTACATGGTATTTCTTTAAATGTAAATATGGACTTAAAACCATTT
AATTTTATTTCTCCTTGTGGAAATAAAAAAATAAAGATGACTCAAATCAAAAATTTTAAAAAAAATATCACAATAAAAAA
TGTTCAAGAAATTTTTATAAAAAATTTTTATATTTTATTTAATTATTAA

Upstream 100 bases:

>100_bases
TAAATTAACATTTTATTAAAAATTTTTTATCTAATTAAATATTAAATTTAATAAATAATTATTTTTATTAATTTATTCAA
TAAATATATAAATTTTTAAA

Downstream 100 bases:

>100_bases
ATTATAAAAACAATTATATATTTAAAAAATATATAATTAATTTTAATAATAAAATATAAATTAGAGAAAAATTATAATGT
TAAAAGAAAAAAAAGATAAA

Product: hypothetical protein

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 202; Mature: 202

Protein sequence:

>202_residues
MNIIIRNLGICNWLGIEEKMIKFTKNRSFNTSDELWCLEHYPVFTYGVLEKKKYAPFIHKIPVFKSNRGGKITFHGPGQL
IIYLLIDLRRKKIKFYELVNRIEILIIHILKILKISSYTKKKHPGIYVKKKKICSLGFRIINGCSLHGISLNVNMDLKPF
NFISPCGNKKIKMTQIKNFKKNITIKNVQEIFIKNFYILFNY

Sequences:

>Translated_202_residues
MNIIIRNLGICNWLGIEEKMIKFTKNRSFNTSDELWCLEHYPVFTYGVLEKKKYAPFIHKIPVFKSNRGGKITFHGPGQL
IIYLLIDLRRKKIKFYELVNRIEILIIHILKILKISSYTKKKHPGIYVKKKKICSLGFRIINGCSLHGISLNVNMDLKPF
NFISPCGNKKIKMTQIKNFKKNITIKNVQEIFIKNFYILFNY
>Mature_202_residues
MNIIIRNLGICNWLGIEEKMIKFTKNRSFNTSDELWCLEHYPVFTYGVLEKKKYAPFIHKIPVFKSNRGGKITFHGPGQL
IIYLLIDLRRKKIKFYELVNRIEILIIHILKILKISSYTKKKHPGIYVKKKKICSLGFRIINGCSLHGISLNVNMDLKPF
NFISPCGNKKIKMTQIKNFKKNITIKNVQEIFIKNFYILFNY

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family [H]

Homologues:

Organism=Homo sapiens, GI221554520, Length=169, Percent_Identity=29.585798816568, Blast_Score=76, Evalue=3e-14,
Organism=Escherichia coli, GI87081767, Length=199, Percent_Identity=43.21608040201, Blast_Score=193, Evalue=7e-51,
Organism=Saccharomyces cerevisiae, GI6323268, Length=188, Percent_Identity=30.8510638297872, Blast_Score=79, Evalue=6e-16,
Organism=Drosophila melanogaster, GI21355483, Length=171, Percent_Identity=30.9941520467836, Blast_Score=87, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 23816; Mature: 23816

Theoretical pI: Translated: 10.67; Mature: 10.67

Prosite motif: PS01313 LIPB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIIIRNLGICNWLGIEEKMIKFTKNRSFNTSDELWCLEHYPVFTYGVLEKKKYAPFIHK
CEEEEECCCCEEECCCHHHHHHHHCCCCCCCCCCEEEEECCCEEEEEHHCCCCCCCHHHC
IPVFKSNRGGKITFHGPGQLIIYLLIDLRRKKIKFYELVNRIEILIIHILKILKISSYTK
CCCEECCCCCEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
KKHPGIYVKKKKICSLGFRIINGCSLHGISLNVNMDLKPFNFISPCGNKKIKMTQIKNFK
CCCCCEEEEHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCCCCCCCCCEEEEHHHHHHH
KNITIKNVQEIFIKNFYILFNY
HCCCHHHHHHHHHCEEEEEEEC
>Mature Secondary Structure
MNIIIRNLGICNWLGIEEKMIKFTKNRSFNTSDELWCLEHYPVFTYGVLEKKKYAPFIHK
CEEEEECCCCEEECCCHHHHHHHHCCCCCCCCCCEEEEECCCEEEEEHHCCCCCCCHHHC
IPVFKSNRGGKITFHGPGQLIIYLLIDLRRKKIKFYELVNRIEILIIHILKILKISSYTK
CCCEECCCCCEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
KKHPGIYVKKKKICSLGFRIINGCSLHGISLNVNMDLKPFNFISPCGNKKIKMTQIKNFK
CCCCCEEEEHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCCCCCCCCCEEEEHHHHHHH
KNITIKNVQEIFIKNFYILFNY
HCCCHHHHHHHHHCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10993077 [H]