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The map label for this gene is lipA

Identifier: 116515106

GI number: 116515106

Start: 198016

End: 198948

Strand: Direct

Name: lipA

Synonym: BCc_170

Alternate gene names: 116515106

Gene position: 198016-198948 (Clockwise)

Preceding gene: 116515105

Following gene: 116515110

Centisome position: 47.56

GC content: 23.15

Gene sequence:

>933_bases
ATGTTAAAAGAAAAAAAAGATAAAGAAAAAAATATATACATGATACCAAAAATTTTAAAATCTAGTAAATCGAAATCAAT
TTTAAAAAAACCAAAATGGTTAAAAATTAAACTTCCTTCTAATTTAAAAAAAATTAACAAGATAAAAAAAATATTAAAAA
AAAATTTTTTACATTCTGTCTGTGAGGAAGCCAATTGTCCAAATTTGCCTGAATGTTTTAATAATGGTACAGCAACATTT
ATGATTTTAGGATCTATATGTACTCGTAAATGTCCTTTTTGCGCTGTTACTAAAGGAAGGGCTCAAAAAATAGATAAAAA
TGAACCAAAAAAAATACTTGATATTGTTATTAAATTAAAATTAACATATGTTGTTTTAACATCAGTAGCAAGAGATGATT
TAAAAGATGGCGGAGCTAAACATTTTTCTAAGTGTATTTATGAAATTCGTAAAAAAAAAAATATAAAAGTAGAAATTTTA
GTTCCAGATTTTAGAGGAAAAGAAAAAATAGCATTAAAAATATTTAATAAATTTCCTCCAGATATATTTAATCATAATAT
TGAAAATGTTCCTAGACTTTATTCTTTAATTAGACCTGGAGCAGATTATATTAAATCTTTAAATTTATTATATCAATTTA
AAAAAATTTGCCCGAATATACCAACAAAATCCGGTTTAATGTTGGGTTTAGGTGAAAAAAAAAAAGAAATTATATCAGTG
TTAAAAGATTTAAAATCTGTAGGTGTATCAATTGTTACTATCGGTCAATATTTGCAACCAAGTAAAAATCATTTATTAGT
TCAAAAATATATTACACCTAAAGAATTTAAAAATTTTGAATATATAGCTTTATCTTTAGGATTTTCTAAAGTTTTCTGTG
GTCCTCTTGTTCGTTCTTCTTATCACGCTGATAGACAATATTTATCTTTTTAA

Upstream 100 bases:

>100_bases
TTTTATATTTTATTTAATTATTAAATTATAAAAACAATTATATATTTAAAAAATATATAATTAATTTTAATAATAAAATA
TAAATTAGAGAAAAATTATA

Downstream 100 bases:

>100_bases
AATTTTTATTAAAAAAAACATATTGATATAAACAAAAAATATATAAGAACACCAAAAAATATTATTAATTTTTTGTTGGT
GTTTTATGTATTTTAATTAT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MLKEKKDKEKNIYMIPKILKSSKSKSILKKPKWLKIKLPSNLKKINKIKKILKKNFLHSVCEEANCPNLPECFNNGTATF
MILGSICTRKCPFCAVTKGRAQKIDKNEPKKILDIVIKLKLTYVVLTSVARDDLKDGGAKHFSKCIYEIRKKKNIKVEIL
VPDFRGKEKIALKIFNKFPPDIFNHNIENVPRLYSLIRPGADYIKSLNLLYQFKKICPNIPTKSGLMLGLGEKKKEIISV
LKDLKSVGVSIVTIGQYLQPSKNHLLVQKYITPKEFKNFEYIALSLGFSKVFCGPLVRSSYHADRQYLSF

Sequences:

>Translated_310_residues
MLKEKKDKEKNIYMIPKILKSSKSKSILKKPKWLKIKLPSNLKKINKIKKILKKNFLHSVCEEANCPNLPECFNNGTATF
MILGSICTRKCPFCAVTKGRAQKIDKNEPKKILDIVIKLKLTYVVLTSVARDDLKDGGAKHFSKCIYEIRKKKNIKVEIL
VPDFRGKEKIALKIFNKFPPDIFNHNIENVPRLYSLIRPGADYIKSLNLLYQFKKICPNIPTKSGLMLGLGEKKKEIISV
LKDLKSVGVSIVTIGQYLQPSKNHLLVQKYITPKEFKNFEYIALSLGFSKVFCGPLVRSSYHADRQYLSF
>Mature_310_residues
MLKEKKDKEKNIYMIPKILKSSKSKSILKKPKWLKIKLPSNLKKINKIKKILKKNFLHSVCEEANCPNLPECFNNGTATF
MILGSICTRKCPFCAVTKGRAQKIDKNEPKKILDIVIKLKLTYVVLTSVARDDLKDGGAKHFSKCIYEIRKKKNIKVEIL
VPDFRGKEKIALKIFNKFPPDIFNHNIENVPRLYSLIRPGADYIKSLNLLYQFKKICPNIPTKSGLMLGLGEKKKEIISV
LKDLKSVGVSIVTIGQYLQPSKNHLLVQKYITPKEFKNFEYIALSLGFSKVFCGPLVRSSYHADRQYLSF

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=293, Percent_Identity=43.0034129692833, Blast_Score=242, Evalue=4e-64,
Organism=Homo sapiens, GI37577164, Length=252, Percent_Identity=42.0634920634921, Blast_Score=202, Evalue=3e-52,
Organism=Escherichia coli, GI1786846, Length=302, Percent_Identity=56.953642384106, Blast_Score=381, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI32564533, Length=300, Percent_Identity=43.6666666666667, Blast_Score=229, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6324770, Length=288, Percent_Identity=40.9722222222222, Blast_Score=219, Evalue=3e-58,
Organism=Drosophila melanogaster, GI221513272, Length=301, Percent_Identity=43.1893687707641, Blast_Score=238, Evalue=3e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_BUCCC (Q057Q7)

Other databases:

- EMBL:   CP000263
- RefSeq:   YP_802735.1
- STRING:   Q057Q7
- EnsemblBacteria:   EBBUCT00000001523
- GeneID:   4440781
- GenomeReviews:   CP000263_GR
- KEGG:   bcc:BCc_170
- eggNOG:   COG0320
- GeneTree:   EBGT00050000007940
- HOGENOM:   HBG284542
- OMA:   KVNSGPL
- ProtClustDB:   PRK05481
- BioCyc:   BAPH372461:BCC_170-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 35523; Mature: 35523

Theoretical pI: Translated: 10.56; Mature: 10.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKEKKDKEKNIYMIPKILKSSKSKSILKKPKWLKIKLPSNLKKINKIKKILKKNFLHSV
CCCCCCCCCCCEEECHHHHHCCHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHH
CEEANCPNLPECFNNGTATFMILGSICTRKCPFCAVTKGRAQKIDKNEPKKILDIVIKLK
HHHCCCCCCHHHHCCCCEEEHHHHHHHCCCCCEEEECCCHHHCCCCCCHHHHHHHHHHHH
LTYVVLTSVARDDLKDGGAKHFSKCIYEIRKKKNIKVEILVPDFRGKEKIALKIFNKFPP
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCH
DIFNHNIENVPRLYSLIRPGADYIKSLNLLYQFKKICPNIPTKSGLMLGLGEKKKEIISV
HHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHHHH
LKDLKSVGVSIVTIGQYLQPSKNHLLVQKYITPKEFKNFEYIALSLGFSKVFCGPLVRSS
HHHHHHCCEEEEEHHHHHCCCCCCEEEEECCCHHHHCCCEEEEEECCHHHHHHHHHHHHH
YHADRQYLSF
HHHHHHHHCC
>Mature Secondary Structure
MLKEKKDKEKNIYMIPKILKSSKSKSILKKPKWLKIKLPSNLKKINKIKKILKKNFLHSV
CCCCCCCCCCCEEECHHHHHCCHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHH
CEEANCPNLPECFNNGTATFMILGSICTRKCPFCAVTKGRAQKIDKNEPKKILDIVIKLK
HHHCCCCCCHHHHCCCCEEEHHHHHHHCCCCCEEEECCCHHHCCCCCCHHHHHHHHHHHH
LTYVVLTSVARDDLKDGGAKHFSKCIYEIRKKKNIKVEILVPDFRGKEKIALKIFNKFPP
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCH
DIFNHNIENVPRLYSLIRPGADYIKSLNLLYQFKKICPNIPTKSGLMLGLGEKKKEIISV
HHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCHHHHHHHHH
LKDLKSVGVSIVTIGQYLQPSKNHLLVQKYITPKEFKNFEYIALSLGFSKVFCGPLVRSS
HHHHHHCCEEEEEHHHHHCCCCCCEEEEECCCHHHHCCCEEEEEECCHHHHHHHHHHHHH
YHADRQYLSF
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA