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The map label for this gene is yhhF [H]

Identifier: 116514963

GI number: 116514963

Start: 14426

End: 14992

Strand: Reverse

Name: yhhF [H]

Synonym: BCc_014

Alternate gene names: 116514963

Gene position: 14992-14426 (Counterclockwise)

Preceding gene: 116514967

Following gene: 116514962

Centisome position: 3.6

GC content: 19.58

Gene sequence:

>567_bases
ATGAAAAAAAAAATAAAAAGAAAGAAAATTCGTATTATCAGTGGTTTATATAAAGGTAGAATAATAGATTTAAAAAATAA
AATAAATGTTCGCCCTACTAGAAGCCGAATTAAAGAAACAGTTTTTAGTTGGTTAGATAAATATATAAAAAATTCTGTAT
GTTTAGATTGTTTTGCAGGAAGTGGTAATTTAAGTATTGAATCAGTTTCAAGATTAGCAAAATCAGTTACAGCTTTAGAA
AAAAATTATCTATTAGTAAAAAAATTAAGACATACATTATTAAATTTTTTAATAAAAAATGTATTTGTTCTACGAGTAAA
TACTATAAAATGGTTACAACATTTCGAAAAACCATATGATATTATCTATCTAGATCCACCTTTTAAAAAAAAAGAATTAT
TAAATCAATCAATTATACTTTTAGAAAAATATAGATGGATACATAAAAATTCAATTATATATACTGAACATATTGATAAC
AATATTAATTATCCAAAAAATTGGAAACTAATTAAAAAAAAAAAAATAGGAAATGTATTATTTTCATTATTTTACAAAAA
AAATTAA

Upstream 100 bases:

>100_bases
AAAAAAAATTTTTTTTACCACACATATTAACCTCAAAATTATTTATTATAATAACTCTATTATATCAATAATAAAATACT
TTTATAGTAAAATTATAAAA

Downstream 100 bases:

>100_bases
TAATTAAATATTTAATATTTTAAAAAATTTTATAATAAATATTAATATTTTAATTTTTAAAAATATATATATTTATTTAA
AGATAAATAAATATATTTAA

Product: YhhF

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 188; Mature: 188

Protein sequence:

>188_residues
MKKKIKRKKIRIISGLYKGRIIDLKNKINVRPTRSRIKETVFSWLDKYIKNSVCLDCFAGSGNLSIESVSRLAKSVTALE
KNYLLVKKLRHTLLNFLIKNVFVLRVNTIKWLQHFEKPYDIIYLDPPFKKKELLNQSIILLEKYRWIHKNSIIYTEHIDN
NINYPKNWKLIKKKKIGNVLFSLFYKKN

Sequences:

>Translated_188_residues
MKKKIKRKKIRIISGLYKGRIIDLKNKINVRPTRSRIKETVFSWLDKYIKNSVCLDCFAGSGNLSIESVSRLAKSVTALE
KNYLLVKKLRHTLLNFLIKNVFVLRVNTIKWLQHFEKPYDIIYLDPPFKKKELLNQSIILLEKYRWIHKNSIIYTEHIDN
NINYPKNWKLIKKKKIGNVLFSLFYKKN
>Mature_188_residues
MKKKIKRKKIRIISGLYKGRIIDLKNKINVRPTRSRIKETVFSWLDKYIKNSVCLDCFAGSGNLSIESVSRLAKSVTALE
KNYLLVKKLRHTLLNFLIKNVFVLRVNTIKWLQHFEKPYDIIYLDPPFKKKELLNQSIILLEKYRWIHKNSIIYTEHIDN
NINYPKNWKLIKKKKIGNVLFSLFYKKN

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=181, Percent_Identity=35.3591160220994, Blast_Score=137, Evalue=6e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 22467; Mature: 22467

Theoretical pI: Translated: 10.91; Mature: 10.91

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKIKRKKIRIISGLYKGRIIDLKNKINVRPTRSRIKETVFSWLDKYIKNSVCLDCFAG
CCCHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECC
SGNLSIESVSRLAKSVTALEKNYLLVKKLRHTLLNFLIKNVFVLRVNTIKWLQHFEKPYD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHCCCCE
IIYLDPPFKKKELLNQSIILLEKYRWIHKNSIIYTEHIDNNINYPKNWKLIKKKKIGNVL
EEEECCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHH
FSLFYKKN
HHHHHCCC
>Mature Secondary Structure
MKKKIKRKKIRIISGLYKGRIIDLKNKINVRPTRSRIKETVFSWLDKYIKNSVCLDCFAG
CCCHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECC
SGNLSIESVSRLAKSVTALEKNYLLVKKLRHTLLNFLIKNVFVLRVNTIKWLQHFEKPYD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHCCCCE
IIYLDPPFKKKELLNQSIILLEKYRWIHKNSIIYTEHIDNNINYPKNWKLIKKKKIGNVL
EEEECCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHH
FSLFYKKN
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10993077 [H]