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The map label for this gene is gltA-2 [H]

Identifier: 116327376

GI number: 116327376

Start: 639351

End: 640631

Strand: Direct

Name: gltA-2 [H]

Synonym: LBL_0574

Alternate gene names: 116327376

Gene position: 639351-640631 (Clockwise)

Preceding gene: 116327375

Following gene: 116327377

Centisome position: 17.69

GC content: 43.09

Gene sequence:

>1281_bases
ATGAGTGAATTCATTGAAATCAAAGTTGGGGAAAAGTCCTACCAATTTCCGTTGATTTCCGGAACCGACGGTAAAAAAGG
GATCGATATGCGCGGACTTCATCAAAAAACCGGACTCATTTCGTACGATCCCGGTTTTTTTAACACCGCATACGCGGTTA
GTCGAATTTCCAGAAGAGATCCGGATTCGGGGGAACTTCAATACCGCGGTTACGACGTCGCCGACCTCGTTCAACGTTCT
ACATTTGTGGAAACCAGTTATCTTTTGATCTATGGTAAACTTCCCACCGAACAACAGCTTAAAGACTTTTCTTTAAAACT
TTCCAAACATTCTTTGATTCACGAAGATATGATTAATCTTTTCGACGGGTTTCCGGGCAAGGGACATCCTCTTGCGGTTC
TTTCCGTGATGGTGACTTCTCTTTCCAGTTATTATCCGGAAGAATACGAGGAATCTTTGGATAAAGGGATCGATCATTCC
GCGAGACTTCTTGCTAAGATCAGAACGATTGCCGCCTTTTCTTACAAGAAGATCGTCGGCCAACCTTTCGTGTATCCTTT
GGATAAACATCCTTATTGTACGAACTTTCTTTACATGTTGTTTTCGATTCCGTCCAAAGATTATGTTCCTACGGAGGACA
TCGATCGGATTTTGAACCAACTTTGGATTCTTTATGCGGATCATGAGCAAAACGTTTCCAATACGACGGTGCAGGTGATC
GGTTCTACACAAGCTAACTTATTCGCTTCCATCTCTTCCGCGATCAACGCTCTTTGGGGTTCGAGGGAAGGTGGTCGTCA
GATTGCGGCTGTGGGCTTAATTGAAGATATGCTTCGATCCAGAAAGTCGGTTCCGGAATACTTTGAAAAATTCAAAGGGG
ACTCGGAAAGATTGTTTTCGAACGGATTTGGGCACAAAGCTTACGAGGCGAAAAGCAAAAGAGCTATCATCGCGAGTCAA
TTATTCCATGATTTCTACAAAAAAACCCCGGTAGGTCCGATCGCGGAAGTGGCTCTCAAAATCGAAGAGTATATGCAAAA
TGATGAATATTACATCAATCAAAAATTATATCCGAATCTCGAATTTTACAGCGCTGTAATTTTCCATTCTCTTGGAATTC
CGAAAGAATTATTCACCGCAATGCAGGTGATTGGAAAACTTCCGGGTTGGCTGGCGCATTGGAGAGAGCAAAGAGTTTCC
GGAAACTATAGCAAGGCTCGTCCAAAGCAGATTTATACCGGAGAAATGGGTAGGAAATACGTTCCTCTTTCGGAAAGATA
G

Upstream 100 bases:

>100_bases
AAATTCAAGCGGAAGTTTTATCCGGATGGAATGGAATAAAATTGGTTTTAGCAAAATTAGAAGAATTGACGCATAGGATT
CTTTAAAGTTCTCTATCGGT

Downstream 100 bases:

>100_bases
CTACGATGCAAAACCCGAGATGGAAAGATTGGTTCGCTCAGGCTGAAAGAGATTTGGAATGGGCCAAGGTATCTTTGCAT
TCGGGTTTTTTTGCTCAAAC

Product: citrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MSEFIEIKVGEKSYQFPLISGTDGKKGIDMRGLHQKTGLISYDPGFFNTAYAVSRISRRDPDSGELQYRGYDVADLVQRS
TFVETSYLLIYGKLPTEQQLKDFSLKLSKHSLIHEDMINLFDGFPGKGHPLAVLSVMVTSLSSYYPEEYEESLDKGIDHS
ARLLAKIRTIAAFSYKKIVGQPFVYPLDKHPYCTNFLYMLFSIPSKDYVPTEDIDRILNQLWILYADHEQNVSNTTVQVI
GSTQANLFASISSAINALWGSREGGRQIAAVGLIEDMLRSRKSVPEYFEKFKGDSERLFSNGFGHKAYEAKSKRAIIASQ
LFHDFYKKTPVGPIAEVALKIEEYMQNDEYYINQKLYPNLEFYSAVIFHSLGIPKELFTAMQVIGKLPGWLAHWREQRVS
GNYSKARPKQIYTGEMGRKYVPLSER

Sequences:

>Translated_426_residues
MSEFIEIKVGEKSYQFPLISGTDGKKGIDMRGLHQKTGLISYDPGFFNTAYAVSRISRRDPDSGELQYRGYDVADLVQRS
TFVETSYLLIYGKLPTEQQLKDFSLKLSKHSLIHEDMINLFDGFPGKGHPLAVLSVMVTSLSSYYPEEYEESLDKGIDHS
ARLLAKIRTIAAFSYKKIVGQPFVYPLDKHPYCTNFLYMLFSIPSKDYVPTEDIDRILNQLWILYADHEQNVSNTTVQVI
GSTQANLFASISSAINALWGSREGGRQIAAVGLIEDMLRSRKSVPEYFEKFKGDSERLFSNGFGHKAYEAKSKRAIIASQ
LFHDFYKKTPVGPIAEVALKIEEYMQNDEYYINQKLYPNLEFYSAVIFHSLGIPKELFTAMQVIGKLPGWLAHWREQRVS
GNYSKARPKQIYTGEMGRKYVPLSER
>Mature_425_residues
SEFIEIKVGEKSYQFPLISGTDGKKGIDMRGLHQKTGLISYDPGFFNTAYAVSRISRRDPDSGELQYRGYDVADLVQRST
FVETSYLLIYGKLPTEQQLKDFSLKLSKHSLIHEDMINLFDGFPGKGHPLAVLSVMVTSLSSYYPEEYEESLDKGIDHSA
RLLAKIRTIAAFSYKKIVGQPFVYPLDKHPYCTNFLYMLFSIPSKDYVPTEDIDRILNQLWILYADHEQNVSNTTVQVIG
STQANLFASISSAINALWGSREGGRQIAAVGLIEDMLRSRKSVPEYFEKFKGDSERLFSNGFGHKAYEAKSKRAIIASQL
FHDFYKKTPVGPIAEVALKIEEYMQNDEYYINQKLYPNLEFYSAVIFHSLGIPKELFTAMQVIGKLPGWLAHWREQRVSG
NYSKARPKQIYTGEMGRKYVPLSER

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=400, Percent_Identity=24.75, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1786939, Length=413, Percent_Identity=36.8038740920097, Blast_Score=302, Evalue=2e-83,
Organism=Escherichia coli, GI1786527, Length=368, Percent_Identity=27.9891304347826, Blast_Score=148, Evalue=6e-37,
Organism=Saccharomyces cerevisiae, GI6324328, Length=371, Percent_Identity=25.3369272237197, Blast_Score=84, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6319850, Length=394, Percent_Identity=25.8883248730964, Blast_Score=81, Evalue=3e-16,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- InterPro:   IPR010953 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 48578; Mature: 48447

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEFIEIKVGEKSYQFPLISGTDGKKGIDMRGLHQKTGLISYDPGFFNTAYAVSRISRRD
CCCEEEEEECCCCCCCCEEECCCCCCCCCCCCCHHHCCEEEECCCCCHHHHHHHHHHCCC
PDSGELQYRGYDVADLVQRSTFVETSYLLIYGKLPTEQQLKDFSLKLSKHSLIHEDMINL
CCCCCEEECCCCHHHHHHHHHHHEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
FDGFPGKGHPLAVLSVMVTSLSSYYPEEYEESLDKGIDHSARLLAKIRTIAAFSYKKIVG
HCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHC
QPFVYPLDKHPYCTNFLYMLFSIPSKDYVPTEDIDRILNQLWILYADHEQNVSNTTVQVI
CCCEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHEEEEECCCCCCCCCEEEEE
GSTQANLFASISSAINALWGSREGGRQIAAVGLIEDMLRSRKSVPEYFEKFKGDSERLFS
ECCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHH
NGFGHKAYEAKSKRAIIASQLFHDFYKKTPVGPIAEVALKIEEYMQNDEYYINQKLYPNL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCH
EFYSAVIFHSLGIPKELFTAMQVIGKLPGWLAHWREQRVSGNYSKARPKQIYTGEMGRKY
HHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCC
VPLSER
CCCCCC
>Mature Secondary Structure 
SEFIEIKVGEKSYQFPLISGTDGKKGIDMRGLHQKTGLISYDPGFFNTAYAVSRISRRD
CCEEEEEECCCCCCCCEEECCCCCCCCCCCCCHHHCCEEEECCCCCHHHHHHHHHHCCC
PDSGELQYRGYDVADLVQRSTFVETSYLLIYGKLPTEQQLKDFSLKLSKHSLIHEDMINL
CCCCCEEECCCCHHHHHHHHHHHEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
FDGFPGKGHPLAVLSVMVTSLSSYYPEEYEESLDKGIDHSARLLAKIRTIAAFSYKKIVG
HCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHC
QPFVYPLDKHPYCTNFLYMLFSIPSKDYVPTEDIDRILNQLWILYADHEQNVSNTTVQVI
CCCEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHEEEEECCCCCCCCCEEEEE
GSTQANLFASISSAINALWGSREGGRQIAAVGLIEDMLRSRKSVPEYFEKFKGDSERLFS
ECCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHH
NGFGHKAYEAKSKRAIIASQLFHDFYKKTPVGPIAEVALKIEEYMQNDEYYINQKLYPNL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCH
EFYSAVIFHSLGIPKELFTAMQVIGKLPGWLAHWREQRVSGNYSKARPKQIYTGEMGRKY
HHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCC
VPLSER
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]