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The map label for this gene is azoB [H]

Identifier: 116249207

GI number: 116249207

Start: 591199

End: 592056

Strand: Reverse

Name: azoB [H]

Synonym: pRL120543

Alternate gene names: 116249207

Gene position: 592056-591199 (Counterclockwise)

Preceding gene: 116249209

Following gene: 116249205

Centisome position: 68.05

GC content: 62.47

Gene sequence:

>858_bases
ATGTTTGTGATCACCGGAGTAACAGGCCAAGTCGGAGGCATTGTCGCCGCGCGTCTGCTGAAGGCGGGGGTGCCGATACG
CGCCGTCGCGCGCAATGCGGAGAAAGCAGCGGAATGGAAGGAAAAAGGCGCGGAACTGGCCTTGGCCGAGATGACCGACG
CCGATGCGCTCACCAAGGCTTTCGCCGGCGCCGACGGCGTCTTTCTGCTGATCCCGCCGACCTTCGATCCGACCCCTGGC
TTTATGGAAGTGCGCGCTGTCATTGCGGCCCTGAAGACCGCGCTTCTTTCGTCTCGTCCCGCCAAGGTCGTTTGCCTCTC
CACCATCGGCGCGCAGGCCAGAGAACCGAACCTCTTGAGCCAGCTTGGTCTCGTCGAACAGACGTTATCTGCCTTGCCAA
TGCCAATCGCCTTTCTGCGTGCCGGCTGGTTCATGGAGAACACGGCCTGGGATATCGCGCCGGCGCGCGAGACCTGTGTC
TTCTCGAGTTTCCTGCAGCCGCTCGATCAGCCCTATCCAATGGTTTCTGTCCTCGACGTCGCAGCCAAGGCGGCAGAACT
GCTGCAGCACGAGTGGGTGGGCAAACAGGTCGTCGAGTTGATGGGGCCAGAGGGCGTCACGCCCAACGATATCGCCGCTG
TCCTATCATCCATCCTCGGCAAACCCGTCCGGGCGGAGCCGGTCGCCCGCGACACCTGGGAGCAGATTTTCACCGATCAG
GGCATGCAGAACCCCCTCCCGCGGATGCGTATGATCGATGGCTTCAACGAAGGCTGGATCCGGTTCGAGGGCGAGCCGGT
GAAGGGCACGACCACACTGGACACAGCCATGGGCCGTCTCATTTGTCCCGGCCGATAA

Upstream 100 bases:

>100_bases
ATGTGACAGAAGCGCGCTTCAGTCAAATGCTGCCAAAGGACATGATCAGCATAACAAACCGCCAACCTGCTGGCGGTGCA
GGAAGCAAAGGAGTCTAACC

Downstream 100 bases:

>100_bases
ACAGCTCAAAGGCCGTGGCGGAGAGCAGCCACGCCCTTTCTCATGCAGCCTCTGACAGGGTCGCCCCCGGATTTTGAACT
AACGGACTACCCAAAGACTA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MFVITGVTGQVGGIVAARLLKAGVPIRAVARNAEKAAEWKEKGAELALAEMTDADALTKAFAGADGVFLLIPPTFDPTPG
FMEVRAVIAALKTALLSSRPAKVVCLSTIGAQAREPNLLSQLGLVEQTLSALPMPIAFLRAGWFMENTAWDIAPARETCV
FSSFLQPLDQPYPMVSVLDVAAKAAELLQHEWVGKQVVELMGPEGVTPNDIAAVLSSILGKPVRAEPVARDTWEQIFTDQ
GMQNPLPRMRMIDGFNEGWIRFEGEPVKGTTTLDTAMGRLICPGR

Sequences:

>Translated_285_residues
MFVITGVTGQVGGIVAARLLKAGVPIRAVARNAEKAAEWKEKGAELALAEMTDADALTKAFAGADGVFLLIPPTFDPTPG
FMEVRAVIAALKTALLSSRPAKVVCLSTIGAQAREPNLLSQLGLVEQTLSALPMPIAFLRAGWFMENTAWDIAPARETCV
FSSFLQPLDQPYPMVSVLDVAAKAAELLQHEWVGKQVVELMGPEGVTPNDIAAVLSSILGKPVRAEPVARDTWEQIFTDQ
GMQNPLPRMRMIDGFNEGWIRFEGEPVKGTTTLDTAMGRLICPGR
>Mature_285_residues
MFVITGVTGQVGGIVAARLLKAGVPIRAVARNAEKAAEWKEKGAELALAEMTDADALTKAFAGADGVFLLIPPTFDPTPG
FMEVRAVIAALKTALLSSRPAKVVCLSTIGAQAREPNLLSQLGLVEQTLSALPMPIAFLRAGWFMENTAWDIAPARETCV
FSSFLQPLDQPYPMVSVLDVAAKAAELLQHEWVGKQVVELMGPEGVTPNDIAAVLSSILGKPVRAEPVARDTWEQIFTDQ
GMQNPLPRMRMIDGFNEGWIRFEGEPVKGTTTLDTAMGRLICPGR

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Uses preferentially NADPH rather than NADH as an electron donor for its activity. The enzyme reductively cleaved Orange II and carboxy-Orange II, and can a

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NmrA-type oxidoreductase family. Azoreductase type 3 subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR008030 [H]

Pfam domain/function: PF05368 NmrA [H]

EC number: NA

Molecular weight: Translated: 30624; Mature: 30624

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFVITGVTGQVGGIVAARLLKAGVPIRAVARNAEKAAEWKEKGAELALAEMTDADALTKA
CEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHCCHHHHHHH
FAGADGVFLLIPPTFDPTPGFMEVRAVIAALKTALLSSRPAKVVCLSTIGAQAREPNLLS
HCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEHHCCCCCCCHHHH
QLGLVEQTLSALPMPIAFLRAGWFMENTAWDIAPARETCVFSSFLQPLDQPYPMVSVLDV
HHHHHHHHHHHCCHHHHHHHHCHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHH
AAKAAELLQHEWVGKQVVELMGPEGVTPNDIAAVLSSILGKPVRAEPVARDTWEQIFTDQ
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCC
GMQNPLPRMRMIDGFNEGWIRFEGEPVKGTTTLDTAMGRLICPGR
CCCCCCCHHHHHCCCCCCEEEECCCCCCCCCHHHHHHCCCCCCCC
>Mature Secondary Structure
MFVITGVTGQVGGIVAARLLKAGVPIRAVARNAEKAAEWKEKGAELALAEMTDADALTKA
CEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHCCHHHHHHH
FAGADGVFLLIPPTFDPTPGFMEVRAVIAALKTALLSSRPAKVVCLSTIGAQAREPNLLS
HCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEHHCCCCCCCHHHH
QLGLVEQTLSALPMPIAFLRAGWFMENTAWDIAPARETCVFSSFLQPLDQPYPMVSVLDV
HHHHHHHHHHHCCHHHHHHHHCHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHH
AAKAAELLQHEWVGKQVVELMGPEGVTPNDIAAVLSSILGKPVRAEPVARDTWEQIFTDQ
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCC
GMQNPLPRMRMIDGFNEGWIRFEGEPVKGTTTLDTAMGRLICPGR
CCCCCCCHHHHHCCCCCCEEEECCCCCCCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12147495 [H]