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The map label for this gene is cfxB [H]

Identifier: 116248866

GI number: 116248866

Start: 202765

End: 203829

Strand: Direct

Name: cfxB [H]

Synonym: pRL120196

Alternate gene names: 116248866

Gene position: 202765-203829 (Clockwise)

Preceding gene: 116248865

Following gene: 116248874

Centisome position: 23.31

GC content: 60.47

Gene sequence:

>1065_bases
ATGGCACGCATTACGCTGAGGCAACTGCTCGACCATGCTGCGGAGGAAGGCTACGGCGTTCCCGCTTTCAACATCAATAA
TATGGAGCAGGCGCTCGCCATTATGGAAGCCGCCGACGCGTGTCATGCTCCGGTTATCATGCAGGCGTCGCGCGGAGCCC
GCGCCTACGCCCATGACATCATGCTGAAGCATATGATGGATGCCGTCGTCGAAATCTATCCGCATATTCCGGTCTGCGTG
CATCTCGACCATGGCAACGATCCCTCCAACTGCATGACGGCGATCCAGGCGGGCTTCACCTCGGTGATGATGGATGGTTC
GCTGAAGGCGGACGCCAAGACGCCTGCCGACTGGGCCTATAATGTCGGCGTCACCAAGATGGTGACGGACATGGCGCATT
TCGGCGGCATCTCGGTGGAAGGAGAACTCGGCGTTCTCGGCTCGCTGGAGACGGGCATGGGCGAGGCCGAGGACGGTCAC
GGCGCCGAGGGCAAGCTTTCGCATGACCAGCTGCTGACCGATCCGGACGAAGCCGTGAAGTTCGTGCGCGAAACCAAGGT
CGATGCGCTCGCCATCGCGATGGGCACATCGCATGGCGCCTACAAGTTCACCCGCAAGCCGGACGGTTCGGTGCTGGCGA
TGAACGTCATCGAAGAAATTCACCGTAAGCTCCCGAACACGCATCTCGTCATGCATGGCTCGTCCTCGGTCCCGATCGAG
CTGCAGGAGATCATCAACAAGTATGGCGGCCAGATGAAGCCGACATGGGGAGTGCCGGTCGAGGAAATCCAGCGCGGCAT
CAAGAACGGCGTGCGCAAGGTCAATATCGACACCGATGGCAGAATGGCGATGACCGGTCAGATCCGCCGCGTGCTGCAGG
AGGACCCAAGCGAGTTCGACCCGCGCAAATATCTGAAGCCGGCAATGACGGCGCTGACCAAGCTCTGCAAGGAGCGCTTC
GAACAGTTCGGCACTGCCGGCATGGCCGGCCGCATCACGCCCCTTCCCGTTTCGGAAATGGCGAAGCGTTATAAATCCGG
TTCCCTCGATCCGGCTTTCTCCTGA

Upstream 100 bases:

>100_bases
TTTGCCCATTAAAATAAGACGCTTGAACGGCTCACGTCCGGGCTCTAGTTTCGAAGAGATGGCGGCGTTTCGATCGACCA
TCGATATGGGGGAATCTTGG

Downstream 100 bases:

>100_bases
AACGCTCTAGCCCAACGCCGGGCGGGGTGCCTTTTCCGGCTCGGCGGCGGCAGGCTTGCCTGTCAGTGCTTGGCCGTGGA
AGCGGCTCAGCTGGCTGACA

Product: fructose-1,6-bisphosphate aldolase

Products: NA

Alternate protein names: FBP aldolase; FBPA; Fructose-1,6-bisphosphate aldolase; Fructose-bisphosphate aldolase II [H]

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MARITLRQLLDHAAEEGYGVPAFNINNMEQALAIMEAADACHAPVIMQASRGARAYAHDIMLKHMMDAVVEIYPHIPVCV
HLDHGNDPSNCMTAIQAGFTSVMMDGSLKADAKTPADWAYNVGVTKMVTDMAHFGGISVEGELGVLGSLETGMGEAEDGH
GAEGKLSHDQLLTDPDEAVKFVRETKVDALAIAMGTSHGAYKFTRKPDGSVLAMNVIEEIHRKLPNTHLVMHGSSSVPIE
LQEIINKYGGQMKPTWGVPVEEIQRGIKNGVRKVNIDTDGRMAMTGQIRRVLQEDPSEFDPRKYLKPAMTALTKLCKERF
EQFGTAGMAGRITPLPVSEMAKRYKSGSLDPAFS

Sequences:

>Translated_354_residues
MARITLRQLLDHAAEEGYGVPAFNINNMEQALAIMEAADACHAPVIMQASRGARAYAHDIMLKHMMDAVVEIYPHIPVCV
HLDHGNDPSNCMTAIQAGFTSVMMDGSLKADAKTPADWAYNVGVTKMVTDMAHFGGISVEGELGVLGSLETGMGEAEDGH
GAEGKLSHDQLLTDPDEAVKFVRETKVDALAIAMGTSHGAYKFTRKPDGSVLAMNVIEEIHRKLPNTHLVMHGSSSVPIE
LQEIINKYGGQMKPTWGVPVEEIQRGIKNGVRKVNIDTDGRMAMTGQIRRVLQEDPSEFDPRKYLKPAMTALTKLCKERF
EQFGTAGMAGRITPLPVSEMAKRYKSGSLDPAFS
>Mature_353_residues
ARITLRQLLDHAAEEGYGVPAFNINNMEQALAIMEAADACHAPVIMQASRGARAYAHDIMLKHMMDAVVEIYPHIPVCVH
LDHGNDPSNCMTAIQAGFTSVMMDGSLKADAKTPADWAYNVGVTKMVTDMAHFGGISVEGELGVLGSLETGMGEAEDGHG
AEGKLSHDQLLTDPDEAVKFVRETKVDALAIAMGTSHGAYKFTRKPDGSVLAMNVIEEIHRKLPNTHLVMHGSSSVPIEL
QEIINKYGGQMKPTWGVPVEEIQRGIKNGVRKVNIDTDGRMAMTGQIRRVLQEDPSEFDPRKYLKPAMTALTKLCKERFE
QFGTAGMAGRITPLPVSEMAKRYKSGSLDPAFS

Specific function: Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3- phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis [H]

COG id: COG0191

COG function: function code G; Fructose/tagatose bisphosphate aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class II fructose-bisphosphate aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1789526, Length=337, Percent_Identity=34.1246290801187, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI87082041, Length=329, Percent_Identity=32.5227963525836, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1788072, Length=324, Percent_Identity=28.3950617283951, Blast_Score=99, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006412
- InterPro:   IPR000771 [H]

Pfam domain/function: PF01116 F_bP_aldolase [H]

EC number: =4.1.2.13 [H]

Molecular weight: Translated: 38545; Mature: 38414

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS00602 ALDOLASE_CLASS_II_1 ; PS00806 ALDOLASE_CLASS_II_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
6.2 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
5.9 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARITLRQLLDHAAEEGYGVPAFNINNMEQALAIMEAADACHAPVIMQASRGARAYAHDI
CCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHH
MLKHMMDAVVEIYPHIPVCVHLDHGNDPSNCMTAIQAGFTSVMMDGSLKADAKTPADWAY
HHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEE
NVGVTKMVTDMAHFGGISVEGELGVLGSLETGMGEAEDGHGAEGKLSHDQLLTDPDEAVK
CCCHHHHHHHHHHHCCEEECCCCCCEECHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
FVRETKVDALAIAMGTSHGAYKFTRKPDGSVLAMNVIEEIHRKLPNTHLVMHGSSSVPIE
HHHHHCHHEEEEEECCCCCCEEECCCCCCCEEHHHHHHHHHHHCCCCEEEEECCCCCCCH
LQEIINKYGGQMKPTWGVPVEEIQRGIKNGVRKVNIDTDGRMAMTGQIRRVLQEDPSEFD
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCEEEECCCCCCEEHHHHHHHHHHCCCCCCC
PRKYLKPAMTALTKLCKERFEQFGTAGMAGRITPLPVSEMAKRYKSGSLDPAFS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
ARITLRQLLDHAAEEGYGVPAFNINNMEQALAIMEAADACHAPVIMQASRGARAYAHDI
CCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHH
MLKHMMDAVVEIYPHIPVCVHLDHGNDPSNCMTAIQAGFTSVMMDGSLKADAKTPADWAY
HHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEE
NVGVTKMVTDMAHFGGISVEGELGVLGSLETGMGEAEDGHGAEGKLSHDQLLTDPDEAVK
CCCHHHHHHHHHHHCCEEECCCCCCEECHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
FVRETKVDALAIAMGTSHGAYKFTRKPDGSVLAMNVIEEIHRKLPNTHLVMHGSSSVPIE
HHHHHCHHEEEEEECCCCCCEEECCCCCCCEEHHHHHHHHHHHCCCCEEEEECCCCCCCH
LQEIINKYGGQMKPTWGVPVEEIQRGIKNGVRKVNIDTDGRMAMTGQIRRVLQEDPSEFD
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCEEEECCCCCCEEHHHHHHHHHHCCCCCCC
PRKYLKPAMTALTKLCKERFEQFGTAGMAGRITPLPVSEMAKRYKSGSLDPAFS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1939098 [H]