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The map label for this gene is paaG [C]

Identifier: 114800473

GI number: 114800473

Start: 3446538

End: 3447350

Strand: Reverse

Name: paaG [C]

Synonym: HNE_3284

Alternate gene names: 114800473

Gene position: 3447350-3446538 (Counterclockwise)

Preceding gene: 114799188

Following gene: 114799570

Centisome position: 93.05

GC content: 62.12

Gene sequence:

>813_bases
ATGAGCGCGCATTACGACATCACGGCAGAAAAATGGGAACGTTTCACCTTTGAACGGAAGGGCCGTGTGCTGACGGCCTA
TATCACCTCCGATCATCCGGTGAACGGCGTGGACGAGGCCATGCACACAGAGCTGGCGCTTGTGTTCAACTGCCTGCAAC
GCGACACCGACAGCGACCTCATCATCCTTTCGGCCAAGGGCCGTGCCTTCTGCGCGGGCGGGGATTTTGACTGGTTTGAG
GAACAGATCGCGCATCCTGAACGCTTCCGCGCCATCGGCTGGGACGCCAAGCAGATCGTGGTGACGCTGCTCCAGATGGA
AAAGCCAGTCATTTGCCGGATGAATGGCGCGGCCGCAGGCCTGGGCGCGACGATTGCGCTGCTGTGCGATGTGATCATTG
CCGACGAGACGGCCAAGATCGGCGATCCGCATGTGAAGGTCGGCCTGGTGGCAGGCGATGGCGGCGCGCTGATCTGGCCG
CAGCTGATCGGTTTTGCCAAGGCCAAGGAACTTCTGATGACGGGCGACCTGCTGAGTGCGCCCGAAGCCAAGGCGCTCGG
CCTCATAAATTATGCCGTGCCGGCCGCAGAGCTCGACGCAAAAGTGGACGAGATCGTCGGCAAGATCATGGCCAACCCGA
AATGGGCGGTGCGCTGGACCAAGACGACCGCCAACATCCCCCTGCGCGCGCTCGCCGCGCAGATGATGGATGCGTCCATC
GGCTGGGAAAGCGTCTCAAACTATCTGGGCGACCGGAAAGAGGCCGTGGCCGCTTTCAAGGAACGCCGCGCGGCGAAGCT
GACGGGGGAGTGA

Upstream 100 bases:

>100_bases
TGCGGGAAAATAAGGGCAGGCCGGCCGAAACACGCCGAATTATGACTTTGCCCGCCCGCCTGGCGCAACTAGGCTGGCGC
CAAAGCAGGCCGGAGGAACC

Downstream 100 bases:

>100_bases
GGAAACACTCATCCCATTTGACGCGCCGCCCAACCCGTACTAAATCCTCGTCCGTGGTGATTTGGCCGGCCGGCTTGCAG
CCACGTTAAACAAGTCGCTA

Product: enoyl-CoA hydratase/isomerase domain-containing protein

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MSAHYDITAEKWERFTFERKGRVLTAYITSDHPVNGVDEAMHTELALVFNCLQRDTDSDLIILSAKGRAFCAGGDFDWFE
EQIAHPERFRAIGWDAKQIVVTLLQMEKPVICRMNGAAAGLGATIALLCDVIIADETAKIGDPHVKVGLVAGDGGALIWP
QLIGFAKAKELLMTGDLLSAPEAKALGLINYAVPAAELDAKVDEIVGKIMANPKWAVRWTKTTANIPLRALAAQMMDASI
GWESVSNYLGDRKEAVAAFKERRAAKLTGE

Sequences:

>Translated_270_residues
MSAHYDITAEKWERFTFERKGRVLTAYITSDHPVNGVDEAMHTELALVFNCLQRDTDSDLIILSAKGRAFCAGGDFDWFE
EQIAHPERFRAIGWDAKQIVVTLLQMEKPVICRMNGAAAGLGATIALLCDVIIADETAKIGDPHVKVGLVAGDGGALIWP
QLIGFAKAKELLMTGDLLSAPEAKALGLINYAVPAAELDAKVDEIVGKIMANPKWAVRWTKTTANIPLRALAAQMMDASI
GWESVSNYLGDRKEAVAAFKERRAAKLTGE
>Mature_269_residues
SAHYDITAEKWERFTFERKGRVLTAYITSDHPVNGVDEAMHTELALVFNCLQRDTDSDLIILSAKGRAFCAGGDFDWFEE
QIAHPERFRAIGWDAKQIVVTLLQMEKPVICRMNGAAAGLGATIALLCDVIIADETAKIGDPHVKVGLVAGDGGALIWPQ
LIGFAKAKELLMTGDLLSAPEAKALGLINYAVPAAELDAKVDEIVGKIMANPKWAVRWTKTTANIPLRALAAQMMDASIG
WESVSNYLGDRKEAVAAFKERRAAKLTGE

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=264, Percent_Identity=28.030303030303, Blast_Score=101, Evalue=8e-22,
Organism=Homo sapiens, GI4757966, Length=191, Percent_Identity=31.9371727748691, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI51538581, Length=191, Percent_Identity=31.9371727748691, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI25453481, Length=191, Percent_Identity=31.9371727748691, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI51538573, Length=191, Percent_Identity=31.9371727748691, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI221136756, Length=189, Percent_Identity=31.2169312169312, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI221136753, Length=189, Percent_Identity=31.2169312169312, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI221307494, Length=189, Percent_Identity=31.2169312169312, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI116235487, Length=260, Percent_Identity=28.8461538461538, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI4757968, Length=158, Percent_Identity=32.9113924050633, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI49355787, Length=158, Percent_Identity=32.9113924050633, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI20127408, Length=178, Percent_Identity=28.6516853932584, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI31542718, Length=217, Percent_Identity=28.110599078341, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI68989263, Length=167, Percent_Identity=30.5389221556886, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1787660, Length=256, Percent_Identity=28.90625, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787659, Length=258, Percent_Identity=25.1937984496124, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI221142681, Length=257, Percent_Identity=28.0155642023346, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788682, Length=229, Percent_Identity=26.6375545851528, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788597, Length=172, Percent_Identity=27.3255813953488, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790281, Length=182, Percent_Identity=28.5714285714286, Blast_Score=65, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI25145438, Length=254, Percent_Identity=29.5275590551181, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17554946, Length=240, Percent_Identity=27.5, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17540714, Length=193, Percent_Identity=29.0155440414508, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17535521, Length=219, Percent_Identity=25.1141552511416, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17549921, Length=192, Percent_Identity=28.125, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17560910, Length=170, Percent_Identity=27.0588235294118, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17558304, Length=162, Percent_Identity=27.1604938271605, Blast_Score=67, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=218, Percent_Identity=25.2293577981651, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI20129971, Length=266, Percent_Identity=27.8195488721804, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24653477, Length=266, Percent_Identity=27.8195488721804, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24654903, Length=196, Percent_Identity=26.530612244898, Blast_Score=91, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24653139, Length=266, Percent_Identity=28.9473684210526, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI19922422, Length=242, Percent_Identity=27.2727272727273, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI21357171, Length=166, Percent_Identity=30.7228915662651, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24650670, Length=176, Percent_Identity=24.4318181818182, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 29382; Mature: 29251

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAHYDITAEKWERFTFERKGRVLTAYITSDHPVNGVDEAMHTELALVFNCLQRDTDSDL
CCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCE
IILSAKGRAFCAGGDFDWFEEQIAHPERFRAIGWDAKQIVVTLLQMEKPVICRMNGAAAG
EEEEECCCEEEECCCCHHHHHHHCCHHHHHHCCCCHHHHHHHHHHCCCCEEEEECCCCCC
LGATIALLCDVIIADETAKIGDPHVKVGLVAGDGGALIWPQLIGFAKAKELLMTGDLLSA
HHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCEEHHHHHHHHHHHHHHHHCCCCCCC
PEAKALGLINYAVPAAELDAKVDEIVGKIMANPKWAVRWTKTTANIPLRALAAQMMDASI
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHCC
GWESVSNYLGDRKEAVAAFKERRAAKLTGE
CHHHHHHHHCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SAHYDITAEKWERFTFERKGRVLTAYITSDHPVNGVDEAMHTELALVFNCLQRDTDSDL
CCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCE
IILSAKGRAFCAGGDFDWFEEQIAHPERFRAIGWDAKQIVVTLLQMEKPVICRMNGAAAG
EEEEECCCEEEECCCCHHHHHHHCCHHHHHHCCCCHHHHHHHHHHCCCCEEEEECCCCCC
LGATIALLCDVIIADETAKIGDPHVKVGLVAGDGGALIWPQLIGFAKAKELLMTGDLLSA
HHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCEEHHHHHHHHHHHHHHHHCCCCCCC
PEAKALGLINYAVPAAELDAKVDEIVGKIMANPKWAVRWTKTTANIPLRALAAQMMDASI
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHCC
GWESVSNYLGDRKEAVAAFKERRAAKLTGE
CHHHHHHHHCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA