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The map label for this gene is ptsN [H]

Identifier: 114799188

GI number: 114799188

Start: 3451907

End: 3452374

Strand: Reverse

Name: ptsN [H]

Synonym: HNE_3289

Alternate gene names: 114799188

Gene position: 3452374-3451907 (Counterclockwise)

Preceding gene: 114800119

Following gene: 114800473

Centisome position: 93.18

GC content: 66.67

Gene sequence:

>468_bases
ATGGCCACCGATTTGAGTGCTCTGCTGCAAGGCGGAGTGATCATCCCCTTTCACGAGGCGACGAGCCGTAAGCAGGCTAT
CCATGTCCTGTGCGAAGCGCTCAGCGAGGCGACGGGGATTGGCGCGCGCGCGATTGAAGACGCCGTGATGGAGCGTGAGC
GCCTTGGCTCCACCGGTGTGGGCGAGGGCGTTGCCATTCCCCATGCCCGGCTTTCCGGTCTCACGGCGCCGGTTGGCGGT
TTCCTGCGGCTCGCCCAGGGCGTCGACTTCGAGGCGATCGATGACCGGCCCTGCGACCTGATCTTCATGCTGCTGGCGCC
CGATGGCGCAGGGGCCGACCATCTGCGCGCGCTGGCGCAGGTCAGCCGGTTTTTCCGCCAGGCCGCCGTGCGCGACGGCC
TGCGCAAGGCGCGCAATGAAAATGAGATCAAGTCGCTGATCTGCAAGGACCCGGTCGTCGGGGGCTGA

Upstream 100 bases:

>100_bases
GCCTGAATATGGTCTACCGCCGCCCCGATGGGCATATTGGTTGGGTCGACCCCGCCAACACGCCCCGGAATTAACGCGAC
GCGCCACGGAAAGTTGTTTG

Downstream 100 bases:

>100_bases
TCTGGCCTGAAACCATCCTGCGCCACAAAAAAACCCCGCCTCCAGGAGGCGGGGTTTTTGTTTGGGAGCTGAACGGATCA
GCCTTCGGCAGGGGAGGGCT

Product: sugar phosphotransferase system (PTS),mannose/fructose family protein

Products: protein histidine; sugar phosphate

Alternate protein names: Enzyme IIA-NTR; Nitrogen-metabolic PTS system EIIA component [H]

Number of amino acids: Translated: 155; Mature: 154

Protein sequence:

>155_residues
MATDLSALLQGGVIIPFHEATSRKQAIHVLCEALSEATGIGARAIEDAVMERERLGSTGVGEGVAIPHARLSGLTAPVGG
FLRLAQGVDFEAIDDRPCDLIFMLLAPDGAGADHLRALAQVSRFFRQAAVRDGLRKARNENEIKSLICKDPVVGG

Sequences:

>Translated_155_residues
MATDLSALLQGGVIIPFHEATSRKQAIHVLCEALSEATGIGARAIEDAVMERERLGSTGVGEGVAIPHARLSGLTAPVGG
FLRLAQGVDFEAIDDRPCDLIFMLLAPDGAGADHLRALAQVSRFFRQAAVRDGLRKARNENEIKSLICKDPVVGG
>Mature_154_residues
ATDLSALLQGGVIIPFHEATSRKQAIHVLCEALSEATGIGARAIEDAVMERERLGSTGVGEGVAIPHARLSGLTAPVGGF
LRLAQGVDFEAIDDRPCDLIFMLLAPDGAGADHLRALAQVSRFFRQAAVRDGLRKARNENEIKSLICKDPVVGG

Specific function: Seems to have a role in regulating nitrogen assimilation [H]

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1789597, Length=127, Percent_Identity=33.8582677165354, Blast_Score=87, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR006320 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 16384; Mature: 16253

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATDLSALLQGGVIIPFHEATSRKQAIHVLCEALSEATGIGARAIEDAVMERERLGSTGV
CCCCHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCC
GEGVAIPHARLSGLTAPVGGFLRLAQGVDFEAIDDRPCDLIFMLLAPDGAGADHLRALAQ
CCCCCCCHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHH
VSRFFRQAAVRDGLRKARNENEIKSLICKDPVVGG
HHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ATDLSALLQGGVIIPFHEATSRKQAIHVLCEALSEATGIGARAIEDAVMERERLGSTGV
CCCHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCC
GEGVAIPHARLSGLTAPVGGFLRLAQGVDFEAIDDRPCDLIFMLLAPDGAGADHLRALAQ
CCCCCCCHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHH
VSRFFRQAAVRDGLRKARNENEIKSLICKDPVVGG
HHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275; 1991712 [H]