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The map label for this gene is paaG [C]

Identifier: 114797855

GI number: 114797855

Start: 3520228

End: 3521058

Strand: Reverse

Name: paaG [C]

Synonym: HNE_3375

Alternate gene names: 114797855

Gene position: 3521058-3520228 (Counterclockwise)

Preceding gene: 114798755

Following gene: 114800467

Centisome position: 95.03

GC content: 65.58

Gene sequence:

>831_bases
ATGAGCGCATTTCGGGAAATCCTGACCGACCTTGCAGGGGGCGTGCTGACGATCACGCTGAACCGGCCCGACCGGCTGAA
TGCCTGGACCGGCGTCATGCAGAGCGAGGTGGAGACGGCCATCCGCGCGGGCGGCGCCGATGACGGCGTGCGCTGTATCG
TGCTGACCGGCGCGGGCCGCGGCTTCTGCGCAGGCGCCGACATGAACGGCCTTCAGGCCATCCAGGCGAGCGCCGGGGCG
GACTATAACGACACGAGACCCGCCTCCAAGCCTCAGTCACCTTCGGAGCTGGAGCAGAAATTTCCCGGCCGGTTCGGCCA
CATGTTTGCCTGCCCCAAGCCGATCATCGCGGCGATCAATGGCCCCTGCGCGGGCATCGGGCTGATCCTCACCCTCTTCG
CAGACCTGCGCTACGCCGCCGCTGAAGCGAAATTCACCACCGCCTTTGCCCAGCGCGGCCTGATTGCCGAGCATGGCATT
GCCTGGCAATTGCCGCGCCTGGTGGGCGAGGCCAACGCGCTGGACCTTCTGTTCACGGCGCGAAAGTTCGATGGCGCAGA
GGCCGAGACGCTGGGCCTCGTCAACAAGGCGCTGCCCGGCAGTGAACTGATGGAGTTTGTCGGAGGCGTTGCCCAGCATC
TCGCCACGCAGGTCTCGCCCCGTTCGGTGGCGATCATGAAGCGCCAGATCCGCGAGAGTTATTTCCAGTCCTATGAGGCC
TCGCTCGCCACGGCCGACGCCGAGATGGAAGGCAGCTTCGCCACGCATGATTTCAAGGAAGGCGTCGCCAGTTTCGTTGA
GCGCCGCGCGCCGGCCTTTACCGGCAAATAG

Upstream 100 bases:

>100_bases
GCGGGGGCGGTGTCAAGCAAGGCGCCGCTGCAGATTTGCGTCTGCCCATTGGGCGGAAAGGCGTCTAGACTGGAGGGAAA
TGGAAAGGGAGAGACATCAG

Downstream 100 bases:

>100_bases
GAGCCTCATGACCCGCCGTATCGATATTGTTGAAGTTGGCCCGCGCGACGGGTTGCAGAATGATCCGGCCAATCTGAGCG
TGGATCAGAAGCTGGAATTC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MSAFREILTDLAGGVLTITLNRPDRLNAWTGVMQSEVETAIRAGGADDGVRCIVLTGAGRGFCAGADMNGLQAIQASAGA
DYNDTRPASKPQSPSELEQKFPGRFGHMFACPKPIIAAINGPCAGIGLILTLFADLRYAAAEAKFTTAFAQRGLIAEHGI
AWQLPRLVGEANALDLLFTARKFDGAEAETLGLVNKALPGSELMEFVGGVAQHLATQVSPRSVAIMKRQIRESYFQSYEA
SLATADAEMEGSFATHDFKEGVASFVERRAPAFTGK

Sequences:

>Translated_276_residues
MSAFREILTDLAGGVLTITLNRPDRLNAWTGVMQSEVETAIRAGGADDGVRCIVLTGAGRGFCAGADMNGLQAIQASAGA
DYNDTRPASKPQSPSELEQKFPGRFGHMFACPKPIIAAINGPCAGIGLILTLFADLRYAAAEAKFTTAFAQRGLIAEHGI
AWQLPRLVGEANALDLLFTARKFDGAEAETLGLVNKALPGSELMEFVGGVAQHLATQVSPRSVAIMKRQIRESYFQSYEA
SLATADAEMEGSFATHDFKEGVASFVERRAPAFTGK
>Mature_275_residues
SAFREILTDLAGGVLTITLNRPDRLNAWTGVMQSEVETAIRAGGADDGVRCIVLTGAGRGFCAGADMNGLQAIQASAGAD
YNDTRPASKPQSPSELEQKFPGRFGHMFACPKPIIAAINGPCAGIGLILTLFADLRYAAAEAKFTTAFAQRGLIAEHGIA
WQLPRLVGEANALDLLFTARKFDGAEAETLGLVNKALPGSELMEFVGGVAQHLATQVSPRSVAIMKRQIRESYFQSYEAS
LATADAEMEGSFATHDFKEGVASFVERRAPAFTGK

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=256, Percent_Identity=30.078125, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI70995211, Length=257, Percent_Identity=29.5719844357977, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI45643119, Length=272, Percent_Identity=26.8382352941176, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI260275230, Length=272, Percent_Identity=26.8382352941176, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI260274832, Length=272, Percent_Identity=26.8382352941176, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI31542718, Length=262, Percent_Identity=25.5725190839695, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI4502327, Length=268, Percent_Identity=28.7313432835821, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI150378541, Length=269, Percent_Identity=28.6245353159851, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI25453481, Length=222, Percent_Identity=25.2252252252252, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI51538573, Length=222, Percent_Identity=25.2252252252252, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI4757966, Length=222, Percent_Identity=25.2252252252252, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI51538581, Length=222, Percent_Identity=25.2252252252252, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI116235487, Length=196, Percent_Identity=29.5918367346939, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787660, Length=270, Percent_Identity=33.3333333333333, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1787659, Length=271, Percent_Identity=28.4132841328413, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1788597, Length=263, Percent_Identity=28.5171102661597, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI221142681, Length=285, Percent_Identity=28.7719298245614, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI87082183, Length=269, Percent_Identity=24.907063197026, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788682, Length=219, Percent_Identity=27.3972602739726, Blast_Score=68, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI25145438, Length=269, Percent_Identity=30.8550185873606, Blast_Score=107, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17554946, Length=267, Percent_Identity=28.4644194756554, Blast_Score=100, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17536985, Length=239, Percent_Identity=31.3807531380753, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17534483, Length=263, Percent_Identity=26.2357414448669, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17535521, Length=219, Percent_Identity=27.3972602739726, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17549921, Length=253, Percent_Identity=27.2727272727273, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17560910, Length=197, Percent_Identity=30.9644670050761, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17540714, Length=220, Percent_Identity=27.2727272727273, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI20129971, Length=276, Percent_Identity=31.1594202898551, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24653477, Length=276, Percent_Identity=31.1594202898551, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24653139, Length=268, Percent_Identity=30.2238805970149, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19920382, Length=262, Percent_Identity=29.0076335877863, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24654903, Length=269, Percent_Identity=21.9330855018587, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19922422, Length=272, Percent_Identity=26.4705882352941, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21357171, Length=278, Percent_Identity=28.0575539568345, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 29316; Mature: 29184

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAFREILTDLAGGVLTITLNRPDRLNAWTGVMQSEVETAIRAGGADDGVRCIVLTGAGR
CHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCC
GFCAGADMNGLQAIQASAGADYNDTRPASKPQSPSELEQKFPGRFGHMFACPKPIIAAIN
CEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHCC
GPCAGIGLILTLFADLRYAAAEAKFTTAFAQRGLIAEHGIAWQLPRLVGEANALDLLFTA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHCCCCHHHHHHHH
RKFDGAEAETLGLVNKALPGSELMEFVGGVAQHLATQVSPRSVAIMKRQIRESYFQSYEA
HHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
SLATADAEMEGSFATHDFKEGVASFVERRAPAFTGK
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SAFREILTDLAGGVLTITLNRPDRLNAWTGVMQSEVETAIRAGGADDGVRCIVLTGAGR
HHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCC
GFCAGADMNGLQAIQASAGADYNDTRPASKPQSPSELEQKFPGRFGHMFACPKPIIAAIN
CEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHCC
GPCAGIGLILTLFADLRYAAAEAKFTTAFAQRGLIAEHGIAWQLPRLVGEANALDLLFTA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHCCCCHHHHHHHH
RKFDGAEAETLGLVNKALPGSELMEFVGGVAQHLATQVSPRSVAIMKRQIRESYFQSYEA
HHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
SLATADAEMEGSFATHDFKEGVASFVERRAPAFTGK
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA