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The map label for this gene is 114797105

Identifier: 114797105

GI number: 114797105

Start: 212878

End: 215022

Strand: Direct

Name: 114797105

Synonym: HNE_0221

Alternate gene names: NA

Gene position: 212878-215022 (Clockwise)

Preceding gene: 114799872

Following gene: 114798541

Centisome position: 5.75

GC content: 59.35

Gene sequence:

>2145_bases
ATGAGACATATCAGGCTGTTTGCGGCGGCATTTCTTGTCGCGGCCCAGGTCGGGCTTGCGGCATGCGCCAGTGACGCAGG
TATGGCGCTCGCGCCGCCCGAAGAGGCAGCGCCGCCGGCGGCGGAAGCCGAAATCCTTTGGGACACGTATGGCGTTCCCC
ACATTTATGGTCCGGATGCGGAATCAGTTTTCTATGGATATGGCTGGGCTCAGGTCGCCAGCCACGCCAACACGGTGCTC
CGGCTCTATGGAGAAGCGCGTGGCAAGGGTGCCGAGTATTGGGGCGCGGAGTATGAAGAGACCACCAAATGGCTGGTCAA
GAATGATGTGCCGGGCAGGGCTCAGCTTTGGTATGATGCCTACTCGCCAGAGTTCCGCGCCAAGCTTGATGCGTTTGCGG
AGGGCATGAATGCCTATGCTGCGGCCAATCCCGAGGCCATTGATCCGGAGCTGCGCGTCGTGCTGCCCGTCAGCGGCGTG
GATGTTGTCGCGCATGCGCACCGCCTGATGAATTTTGTCTATGTGGCGTCTCCCGGCCGCGTGGCAGGGGAGGGCGATCC
GCCTGACCTCTCCGAACAGGGGTCGAATACATGGGCGATTTCCGGTTCGAAAACGGCATCCGGCAATACGATGCTGTTGC
AGAACCCGCACCTGCCCTGGGGCACGGGCTATTTCATTTATTATGAGGCGCACCTCAACGGCCCGGACTTTGAAATCTAT
GGCGCCACACAGATCGGCCTGCCGGTGATCCGGTTTGCTTTCAATCAGCAGATGGGTATTTCCAACACCGTGAACGGCAT
GGTGGGCGCAACGACCTATGAGCTGACGCTGAAGGACAATGGCTACCTCTTTGACGGCGAAGTTTTGCCTTTTGATGTCC
GTGAGACGTCCTACAAGCTGCGCCAGGATGACGGCACCCTGCTGGACGTGCCGGTGTCTGTGCGGTCGACCGTACACGGG
CCCGTGTTTGAAAAGGCAGATGGCAAGACCATCGCGCTGCGGGTTGCAGGGCTCGATCGCGGTGGAATGCTGGAGCAGTA
TTTCGACATGGTCACGTCCGACAGCCATGAAGAATTCCAGTCTGCCATGAGCCGTCTGCAGGTGCCGACGTTCAATATTT
CCTATGCCGACCGGGACGGGCATATCGAGTATATTTTCAATGGCATCGCGCCCAAACGCCCTTCAGGCGATATTGCCTTC
TGGCAGGGGCTCGTGCCCGGAGACACGTCTGAATATCTGTGGACCGACATTCATTCGTTTGAAGATCTGCCGCGGGTAAC
AGATCCGGAAACCGGCTTCATTCAGAACTCCAATGACCCGCCTTGGGAGGCGACCTATCCGGTCGCTTACAAGCCTGAGG
ACTACCCAGCCTACTTTGCGCCGCGCACACCGCTTTCCATGCGGGCGCAGGAGTCGATCCGCATGATGACAGAAGCGGAA
GACCTGACGCTGGACGAACTTGTAACGTTGAAGCTCTCCGCCAATGCGCTGATGGCAGACCGGGTGCTGCCCGATCTGCT
GGCTGCGGCGGCGAGTGATCCAGATTCGGATATGCAGGCAGCGGTGAAAGTGCTGTCCGAATGGGATCGCACATTCTCCA
GCGAAAATCGCGCCGGGGTCCTGTTTGAGGAATGGGGCAAGCTGTTTGCTGGCCCGCGCATGACCGGTCAGTCCGGATTT
GCCGTGCCCTGGTCGGCAGATGATCCGATCAATACGCCCTCCGGCTTGAAAGATCCGGCGGCCTCTGTCGAGCTTCTTCG
TCAGGCCATCGCCTCGACCAAGGAGAAGTATGGTCAGATCGATCCGGTCTATGGAGATGTGTCGCGCTTCATCCTTGAAG
ATGTGGATGTGCCGGGGCTCGGCGGATACGGGAACCTGGGCGCGTTCCATGTGATTACCTGGTCGGACCCGAATGCAGAC
GGCGTGCGCACGCCTAACCATGGCGAGACCTGGGTGGCGATGATCGAGTTTTCCACGCCGGTAAAGGCTTATGGCCTCAT
GAGCTATGGGAACTCCCGGCAGCCCGGCACGAGCCACTACAGCGATCAGCTCAACATGCTGTCAGAGGAAAAGTACCGCG
AACTCTGGCTGCAACGCGAGCAGGTGGAAGCCAACCTGGCAGAGCGCACTCCGCTCAACCGATAG

Upstream 100 bases:

>100_bases
ATATAAGTGGTGATCTGACAGGGCTGGCCCGGTGGCGAGGTGCTTCCGGGCCAATCTTTTTACGACAGTCATGACAAGAA
TAAATGAGAGGATTTAAGGG

Downstream 100 bases:

>100_bases
TCCCAATACGGATATACCGAGATGAAACACTATCTGAAATACTTGCCTGCGGTTCTGCCGCTTATAGCGGCAGCCTGCGC
GGCGGCGCCGGAAACTCCGC

Product: penicillin amidase

Products: NA

Alternate protein names: Glutaryl-7-ACA acylase; 7-beta-(4-carboxybutanamido)cephalosporanic acid acylase; CAD; GL-7-ACA acylase; GCA; Glutaryl-7-ACA acylase subunit alpha; Glutaryl-7-ACA acylase subunit beta [H]

Number of amino acids: Translated: 714; Mature: 714

Protein sequence:

>714_residues
MRHIRLFAAAFLVAAQVGLAACASDAGMALAPPEEAAPPAAEAEILWDTYGVPHIYGPDAESVFYGYGWAQVASHANTVL
RLYGEARGKGAEYWGAEYEETTKWLVKNDVPGRAQLWYDAYSPEFRAKLDAFAEGMNAYAAANPEAIDPELRVVLPVSGV
DVVAHAHRLMNFVYVASPGRVAGEGDPPDLSEQGSNTWAISGSKTASGNTMLLQNPHLPWGTGYFIYYEAHLNGPDFEIY
GATQIGLPVIRFAFNQQMGISNTVNGMVGATTYELTLKDNGYLFDGEVLPFDVRETSYKLRQDDGTLLDVPVSVRSTVHG
PVFEKADGKTIALRVAGLDRGGMLEQYFDMVTSDSHEEFQSAMSRLQVPTFNISYADRDGHIEYIFNGIAPKRPSGDIAF
WQGLVPGDTSEYLWTDIHSFEDLPRVTDPETGFIQNSNDPPWEATYPVAYKPEDYPAYFAPRTPLSMRAQESIRMMTEAE
DLTLDELVTLKLSANALMADRVLPDLLAAAASDPDSDMQAAVKVLSEWDRTFSSENRAGVLFEEWGKLFAGPRMTGQSGF
AVPWSADDPINTPSGLKDPAASVELLRQAIASTKEKYGQIDPVYGDVSRFILEDVDVPGLGGYGNLGAFHVITWSDPNAD
GVRTPNHGETWVAMIEFSTPVKAYGLMSYGNSRQPGTSHYSDQLNMLSEEKYRELWLQREQVEANLAERTPLNR

Sequences:

>Translated_714_residues
MRHIRLFAAAFLVAAQVGLAACASDAGMALAPPEEAAPPAAEAEILWDTYGVPHIYGPDAESVFYGYGWAQVASHANTVL
RLYGEARGKGAEYWGAEYEETTKWLVKNDVPGRAQLWYDAYSPEFRAKLDAFAEGMNAYAAANPEAIDPELRVVLPVSGV
DVVAHAHRLMNFVYVASPGRVAGEGDPPDLSEQGSNTWAISGSKTASGNTMLLQNPHLPWGTGYFIYYEAHLNGPDFEIY
GATQIGLPVIRFAFNQQMGISNTVNGMVGATTYELTLKDNGYLFDGEVLPFDVRETSYKLRQDDGTLLDVPVSVRSTVHG
PVFEKADGKTIALRVAGLDRGGMLEQYFDMVTSDSHEEFQSAMSRLQVPTFNISYADRDGHIEYIFNGIAPKRPSGDIAF
WQGLVPGDTSEYLWTDIHSFEDLPRVTDPETGFIQNSNDPPWEATYPVAYKPEDYPAYFAPRTPLSMRAQESIRMMTEAE
DLTLDELVTLKLSANALMADRVLPDLLAAAASDPDSDMQAAVKVLSEWDRTFSSENRAGVLFEEWGKLFAGPRMTGQSGF
AVPWSADDPINTPSGLKDPAASVELLRQAIASTKEKYGQIDPVYGDVSRFILEDVDVPGLGGYGNLGAFHVITWSDPNAD
GVRTPNHGETWVAMIEFSTPVKAYGLMSYGNSRQPGTSHYSDQLNMLSEEKYRELWLQREQVEANLAERTPLNR
>Mature_714_residues
MRHIRLFAAAFLVAAQVGLAACASDAGMALAPPEEAAPPAAEAEILWDTYGVPHIYGPDAESVFYGYGWAQVASHANTVL
RLYGEARGKGAEYWGAEYEETTKWLVKNDVPGRAQLWYDAYSPEFRAKLDAFAEGMNAYAAANPEAIDPELRVVLPVSGV
DVVAHAHRLMNFVYVASPGRVAGEGDPPDLSEQGSNTWAISGSKTASGNTMLLQNPHLPWGTGYFIYYEAHLNGPDFEIY
GATQIGLPVIRFAFNQQMGISNTVNGMVGATTYELTLKDNGYLFDGEVLPFDVRETSYKLRQDDGTLLDVPVSVRSTVHG
PVFEKADGKTIALRVAGLDRGGMLEQYFDMVTSDSHEEFQSAMSRLQVPTFNISYADRDGHIEYIFNGIAPKRPSGDIAF
WQGLVPGDTSEYLWTDIHSFEDLPRVTDPETGFIQNSNDPPWEATYPVAYKPEDYPAYFAPRTPLSMRAQESIRMMTEAE
DLTLDELVTLKLSANALMADRVLPDLLAAAASDPDSDMQAAVKVLSEWDRTFSSENRAGVLFEEWGKLFAGPRMTGQSGF
AVPWSADDPINTPSGLKDPAASVELLRQAIASTKEKYGQIDPVYGDVSRFILEDVDVPGLGGYGNLGAFHVITWSDPNAD
GVRTPNHGETWVAMIEFSTPVKAYGLMSYGNSRQPGTSHYSDQLNMLSEEKYRELWLQREQVEANLAERTPLNR

Specific function: Catalyzes the deacylation of 7 beta-(4- carboxybutanamido)cephalosporanic acid (glutaryl-7- aminocephalosporanic acid or GL-7-ACA) to 7-aminocephalosporanic acid (7-ACA). Can not efficiently use cephalosporin C (CPC), penicillin G, or ampicillin as substr

COG id: COG2366

COG function: function code R; Protein related to penicillin acylase

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S45 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014395
- InterPro:   IPR002692 [H]

Pfam domain/function: PF01804 Penicil_amidase [H]

EC number: =3.5.1.93 [H]

Molecular weight: Translated: 78539; Mature: 78539

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHIRLFAAAFLVAAQVGLAACASDAGMALAPPEEAAPPAAEAEILWDTYGVPHIYGPDA
CCHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCHHCCCCCCCCEEEEECCCCCEEECCCC
ESVFYGYGWAQVASHANTVLRLYGEARGKGAEYWGAEYEETTKWLVKNDVPGRAQLWYDA
CCEEEECCHHHHHHHHHHHHHEEHHHCCCCCCCCCCCHHHHHHHHEECCCCCCEEEEEEC
YSPEFRAKLDAFAEGMNAYAAANPEAIDPELRVVLPVSGVDVVAHAHRLMNFVYVASPGR
CCCHHHHHHHHHHHCCHHHHCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHEEEEECCCC
VAGEGDPPDLSEQGSNTWAISGSKTASGNTMLLQNPHLPWGTGYFIYYEAHLNGPDFEIY
CCCCCCCCCCHHCCCCEEEECCCCCCCCCEEEEECCCCCCCCCEEEEEEEECCCCCEEEE
GATQIGLPVIRFAFNQQMGISNTVNGMVGATTYELTLKDNGYLFDGEVLPFDVRETSYKL
ECHHHCHHHHHHHHCCCCCCCHHCCCCCCCEEEEEEECCCCEEECCCEEEECCCCCCEEE
RQDDGTLLDVPVSVRSTVHGPVFEKADGKTIALRVAGLDRGGMLEQYFDMVTSDSHEEFQ
ECCCCCEEEECHHHHHCCCCCCEECCCCCEEEEEEECCCCCCHHHHHHHHHCCCCHHHHH
SAMSRLQVPTFNISYADRDGHIEYIFNGIAPKRPSGDIAFWQGLVPGDTSEYLWTDIHSF
HHHHHHCCCEEEEEECCCCCCEEEEEECCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHH
EDLPRVTDPETGFIQNSNDPPWEATYPVAYKPEDYPAYFAPRTPLSMRAQESIRMMTEAE
HCCCCCCCCCCCCEECCCCCCCCCCCCEEECCCCCCEEECCCCCHHHHHHHHHHHHHHHC
DLTLDELVTLKLSANALMADRVLPDLLAAAASDPDSDMQAAVKVLSEWDRTFSSENRAGV
CCCHHHEEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
LFEEWGKLFAGPRMTGQSGFAVPWSADDPINTPSGLKDPAASVELLRQAIASTKEKYGQI
HHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
DPVYGDVSRFILEDVDVPGLGGYGNLGAFHVITWSDPNADGVRTPNHGETWVAMIEFSTP
CCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEEEECCC
VKAYGLMSYGNSRQPGTSHYSDQLNMLSEEKYRELWLQREQVEANLAERTPLNR
HHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRHIRLFAAAFLVAAQVGLAACASDAGMALAPPEEAAPPAAEAEILWDTYGVPHIYGPDA
CCHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCHHCCCCCCCCEEEEECCCCCEEECCCC
ESVFYGYGWAQVASHANTVLRLYGEARGKGAEYWGAEYEETTKWLVKNDVPGRAQLWYDA
CCEEEECCHHHHHHHHHHHHHEEHHHCCCCCCCCCCCHHHHHHHHEECCCCCCEEEEEEC
YSPEFRAKLDAFAEGMNAYAAANPEAIDPELRVVLPVSGVDVVAHAHRLMNFVYVASPGR
CCCHHHHHHHHHHHCCHHHHCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHEEEEECCCC
VAGEGDPPDLSEQGSNTWAISGSKTASGNTMLLQNPHLPWGTGYFIYYEAHLNGPDFEIY
CCCCCCCCCCHHCCCCEEEECCCCCCCCCEEEEECCCCCCCCCEEEEEEEECCCCCEEEE
GATQIGLPVIRFAFNQQMGISNTVNGMVGATTYELTLKDNGYLFDGEVLPFDVRETSYKL
ECHHHCHHHHHHHHCCCCCCCHHCCCCCCCEEEEEEECCCCEEECCCEEEECCCCCCEEE
RQDDGTLLDVPVSVRSTVHGPVFEKADGKTIALRVAGLDRGGMLEQYFDMVTSDSHEEFQ
ECCCCCEEEECHHHHHCCCCCCEECCCCCEEEEEEECCCCCCHHHHHHHHHCCCCHHHHH
SAMSRLQVPTFNISYADRDGHIEYIFNGIAPKRPSGDIAFWQGLVPGDTSEYLWTDIHSF
HHHHHHCCCEEEEEECCCCCCEEEEEECCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHH
EDLPRVTDPETGFIQNSNDPPWEATYPVAYKPEDYPAYFAPRTPLSMRAQESIRMMTEAE
HCCCCCCCCCCCCEECCCCCCCCCCCCEEECCCCCCEEECCCCCHHHHHHHHHHHHHHHC
DLTLDELVTLKLSANALMADRVLPDLLAAAASDPDSDMQAAVKVLSEWDRTFSSENRAGV
CCCHHHEEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
LFEEWGKLFAGPRMTGQSGFAVPWSADDPINTPSGLKDPAASVELLRQAIASTKEKYGQI
HHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
DPVYGDVSRFILEDVDVPGLGGYGNLGAFHVITWSDPNADGVRTPNHGETWVAMIEFSTP
CCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEEEECCC
VKAYGLMSYGNSRQPGTSHYSDQLNMLSEEKYRELWLQREQVEANLAERTPLNR
HHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11080627 [H]