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The map label for this gene is pyrC

Identifier: 114331644

GI number: 114331644

Start: 1743551

End: 1744588

Strand: Reverse

Name: pyrC

Synonym: Neut_1659

Alternate gene names: 114331644

Gene position: 1744588-1743551 (Counterclockwise)

Preceding gene: 114331645

Following gene: 114331643

Centisome position: 65.56

GC content: 50.77

Gene sequence:

>1038_bases
ATGACAAACAAGCTGACGTTTACCCGCCCGGATGACTGGCATTTGCATCTGCGTGATGGCAAGGCTATGCAGTCAGTCCT
ACCTGATACTGCTCGGCGTTTTGCGCGCGCCATGATCATGCCGAATCTCAAACTGCCCGTGGTAACGACTGAACAGGCGG
CTGCCTATCGTGCCCGTATCTTGTCTGCCTTGCCACAGGAATTGGTAGGCCAGTTCGAGCCGCTAATGACACTATACCTG
ACCGATACCACTACACCTGAAGAAATTAGCCGAGCAAAGGCGAGCGGGATTGTACAGGCAGTTAAGCTGTATCCGGCCGG
GGCGACCACACATTCCGATGCTGGTGTTACTGATATTGACCGCTGTAAGGCCACACTGGCGATGATGGAGAAGCTTGATA
TGCCTTTGCTTGTGCATGGAGAAGTGGTTGATCCTGCAGTGGATATTTTTGATCGTGAAAAAATTTTTATAGATCGGGTG
CTGATCCCACTTTTACAGCGTTTCCCGGGGTTGAGGGTGGTATTTGAACATATCACCACTCGAGAGGCGGTTGAGTTTGT
GCAGTCGATTTCCAACCGGATTGCTGCCACGATTACCGCGCATCATCTAATGCTGAACCGCAACGCTTTATTTACAGGTG
GTTTACAACCACATCATTACTGCCTGCCGGTGCTAAAGCGGGAAATTCATCGCCAGGCGCTGGTAGCAGCAGCAACCAGC
GGTCATTCTCGTTTCTTTCTCGGTACTGACAGTGCGCCACACCCACTAAAAGATAAGGAATCAGCCTGTGGTTGTGCGGG
TATTTACAGCGCCCACGCAGCGATTGAATTTTATGCTGAAATATTTGAGCAGGCTGGAAGGCTTGATCGGCTAGAGGCAT
TCGCCAGTTTCTACGGCCCTGATTTTTATGGCTTGCCGCGCAACACGGATCAAATTTCATTAAGCAAAGAAAGCTGGCAA
ATACCTGGAGAGGTCGAGTTTGGTGGAGACAGGTTAGTGCCACTTAGGGCAGGAGAACAGGTCTGCTGGAGGTTATGA

Upstream 100 bases:

>100_bases
TGACAAATCCAGCCAGATTTGGAAATTTTTACTGCTGTGGAGTTCTGGAGGAGAATTTGTGTTTGTTTATTGATTTGTGA
GGAAAACCGGATTGAACGAT

Downstream 100 bases:

>100_bases
TGGAGAGTGGATTTATTTTGATTCATCAGATAAGTGCGAATATACTGTATGGATGATTATTGTGATTGGTTCCTGAATCA
AGCAACCTGAACAATCCGCT

Product: dihydroorotase

Products: NA

Alternate protein names: DHOase

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MTNKLTFTRPDDWHLHLRDGKAMQSVLPDTARRFARAMIMPNLKLPVVTTEQAAAYRARILSALPQELVGQFEPLMTLYL
TDTTTPEEISRAKASGIVQAVKLYPAGATTHSDAGVTDIDRCKATLAMMEKLDMPLLVHGEVVDPAVDIFDREKIFIDRV
LIPLLQRFPGLRVVFEHITTREAVEFVQSISNRIAATITAHHLMLNRNALFTGGLQPHHYCLPVLKREIHRQALVAAATS
GHSRFFLGTDSAPHPLKDKESACGCAGIYSAHAAIEFYAEIFEQAGRLDRLEAFASFYGPDFYGLPRNTDQISLSKESWQ
IPGEVEFGGDRLVPLRAGEQVCWRL

Sequences:

>Translated_345_residues
MTNKLTFTRPDDWHLHLRDGKAMQSVLPDTARRFARAMIMPNLKLPVVTTEQAAAYRARILSALPQELVGQFEPLMTLYL
TDTTTPEEISRAKASGIVQAVKLYPAGATTHSDAGVTDIDRCKATLAMMEKLDMPLLVHGEVVDPAVDIFDREKIFIDRV
LIPLLQRFPGLRVVFEHITTREAVEFVQSISNRIAATITAHHLMLNRNALFTGGLQPHHYCLPVLKREIHRQALVAAATS
GHSRFFLGTDSAPHPLKDKESACGCAGIYSAHAAIEFYAEIFEQAGRLDRLEAFASFYGPDFYGLPRNTDQISLSKESWQ
IPGEVEFGGDRLVPLRAGEQVCWRL
>Mature_344_residues
TNKLTFTRPDDWHLHLRDGKAMQSVLPDTARRFARAMIMPNLKLPVVTTEQAAAYRARILSALPQELVGQFEPLMTLYLT
DTTTPEEISRAKASGIVQAVKLYPAGATTHSDAGVTDIDRCKATLAMMEKLDMPLLVHGEVVDPAVDIFDREKIFIDRVL
IPLLQRFPGLRVVFEHITTREAVEFVQSISNRIAATITAHHLMLNRNALFTGGLQPHHYCLPVLKREIHRQALVAAATSG
HSRFFLGTDSAPHPLKDKESACGCAGIYSAHAAIEFYAEIFEQAGRLDRLEAFASFYGPDFYGLPRNTDQISLSKESWQI
PGEVEFGGDRLVPLRAGEQVCWRL

Specific function: Pyrimidine biosynthesis; third step. [C]

COG id: COG0418

COG function: function code F; Dihydroorotase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHOase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787301, Length=341, Percent_Identity=52.7859237536657, Blast_Score=362, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6323452, Length=358, Percent_Identity=33.5195530726257, Blast_Score=190, Evalue=3e-49,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRC_NITEC (Q0AFI1)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_747866.1
- ProteinModelPortal:   Q0AFI1
- SMR:   Q0AFI1
- STRING:   Q0AFI1
- GeneID:   4273812
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_1659
- NMPDR:   fig|335283.3.peg.2200
- eggNOG:   COG0418
- HOGENOM:   HBG628648
- OMA:   KESACGC
- PhylomeDB:   Q0AFI1
- ProtClustDB:   PRK05451
- BioCyc:   NEUT335283:NEUT_1659-MONOMER
- HAMAP:   MF_00219
- InterPro:   IPR006680
- InterPro:   IPR004721
- InterPro:   IPR002195
- PIRSF:   PIRSF001237
- TIGRFAMs:   TIGR00856

Pfam domain/function: PF01979 Amidohydro_1

EC number: =3.5.2.3

Molecular weight: Translated: 38477; Mature: 38346

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: PS00482 DIHYDROOROTASE_1; PS00483 DIHYDROOROTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNKLTFTRPDDWHLHLRDGKAMQSVLPDTARRFARAMIMPNLKLPVVTTEQAAAYRARI
CCCCEEEECCCCCEEEECCCHHHHHHCHHHHHHHHHHHHCCCCCCCEEECHHHHHHHHHH
LSALPQELVGQFEPLMTLYLTDTTTPEEISRAKASGIVQAVKLYPAGATTHSDAGVTDID
HHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCHHH
RCKATLAMMEKLDMPLLVHGEVVDPAVDIFDREKIFIDRVLIPLLQRFPGLRVVFEHITT
HHHHHHHHHHHCCCCEEEECCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHH
REAVEFVQSISNRIAATITAHHLMLNRNALFTGGLQPHHYCLPVLKREIHRQALVAAATS
HHHHHHHHHHHHHHHHHHHHHHHHEECCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCC
GHSRFFLGTDSAPHPLKDKESACGCAGIYSAHAAIEFYAEIFEQAGRLDRLEAFASFYGP
CCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
DFYGLPRNTDQISLSKESWQIPGEVEFGGDRLVPLRAGEQVCWRL
CCCCCCCCCCCEEECCCCCCCCCEEECCCCEEEEECCCCHHHHCC
>Mature Secondary Structure 
TNKLTFTRPDDWHLHLRDGKAMQSVLPDTARRFARAMIMPNLKLPVVTTEQAAAYRARI
CCCEEEECCCCCEEEECCCHHHHHHCHHHHHHHHHHHHCCCCCCCEEECHHHHHHHHHH
LSALPQELVGQFEPLMTLYLTDTTTPEEISRAKASGIVQAVKLYPAGATTHSDAGVTDID
HHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCHHH
RCKATLAMMEKLDMPLLVHGEVVDPAVDIFDREKIFIDRVLIPLLQRFPGLRVVFEHITT
HHHHHHHHHHHCCCCEEEECCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHH
REAVEFVQSISNRIAATITAHHLMLNRNALFTGGLQPHHYCLPVLKREIHRQALVAAATS
HHHHHHHHHHHHHHHHHHHHHHHHEECCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCC
GHSRFFLGTDSAPHPLKDKESACGCAGIYSAHAAIEFYAEIFEQAGRLDRLEAFASFYGP
CCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
DFYGLPRNTDQISLSKESWQIPGEVEFGGDRLVPLRAGEQVCWRL
CCCCCCCCCCCEEECCCCCCCCCEEECCCCEEEEECCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA