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The map label for this gene is glyA

Identifier: 114331539

GI number: 114331539

Start: 1622513

End: 1623763

Strand: Reverse

Name: glyA

Synonym: Neut_1552

Alternate gene names: 114331539

Gene position: 1623763-1622513 (Counterclockwise)

Preceding gene: 114331541

Following gene: 114331538

Centisome position: 61.02

GC content: 51.56

Gene sequence:

>1251_bases
ATGTTTTCAAAGAGTATGACGATTGAGCAGGTTGATCCGGATTTATGGCGAGCGATCCAGGGGGAGGTACAGCGGCAGGA
AGATCATATCGAGCTGATTGCCTCGGAAAATTATGCCAGCCCGGCTGTTTTGCAGGCACAGGGCACTGTGCTGACCAACA
AGTATGCCGAAGGATATCCGGGCAAGCGGTATTACGGAGGATGCAAGCATGTAGATATCGTCGAACAGCTTGCCATTGAT
CGCCTGCGGGCGCTGTTTAATGCCGAATACGTTAACGTGCAGCCGCATTCCGGTTCCCAGGCAAATGCTGCGGTTTATTT
GTCCGCGCTGAAGCCGGGCGATACGCTTCTGGGGATGTCACTGGCGCATGGTGGCCATTTGACGCACGGCGCACCAGTCA
ATATGAGTGGCAAGATATTCAATTCAATTGCTTACGGTCTGGATCCTGAAACTGAAGAGATTGATTACACAGAGCTGGAG
CAGCTTGCGCATGAGCATAAACCTCGCATGATCGTTGCTGGCGCATCTTCCTATGCACGAGTAATTGACTGGCAAGCTTT
CCGCAAAATTGCCGACAACGTAGGTGCCTATCTGTTTGTGGACATGGCTCATTATGCAGGGCTGATTGCTGCCGGTTATT
ATCCCAATCCAGTGGGAATCGCCGATTTTGTTACCAGTAGCACGCACAAAACACTACGTGGCCCGCGTGGTGGCGTGATC
ATGGCTAAGCCTGAGCATGAAAAAGCACTCAACTCAGCAGTTTTCCCGCAGACACAGGGTGGCCCACTGATGCATGTGAT
CGCGGCCAAGGCTGTGGCTTTCAAGGAGGCAGCCAGCCCGGCATTCAAGGATTATCAAAAGCAGGTAATCGAGAACGCGC
GTGTCATGGCAAGAGTATTACAACAGCGCGGCTTGCGGATTGTATCCGGCCATACTGATTGCCATATGTTTCTGGTGGAT
TTACGCGCCAAAAATCTGACCGGAAGGGAGGCGGAGACTGCATTGGAAACCGCGCACATCACCGTCAATAAAAATGCAAT
TCCGAATGATCCGCAAAAACCGTTTGTAACCAGCGGTGTCCGAATTGGTACGCCGGCGATTACCACACGCGGATTTAAGG
AACTTGAATCCGAGCAGCTTGCAAATCTGGTGGCAGATGTGTTGGAAGCTCCGACTAATGAGGCGGTGCTGGATCAGGTT
GCCAGAGAAGCACAAGCTTTGTGTGCAAAATTTCCGGTGTATGGAAATTGA

Upstream 100 bases:

>100_bases
TCGTTTTTGTACGCGTGGCATAGTAATAGAATTACGAAGTTTGCGCCGCGACGCGATGATAGAATAATCATTTTCCAATA
AAAATCAGAGAGTAAATACT

Downstream 100 bases:

>100_bases
TTATTTTGTCTTGTGCACCAGGTTTCTGTGTCGGCTATCCGGTTCCAGTTACCTGTAATTTTGGAGGTACAGAGCAATGC
GATGCCCGTTCTGCGGTGCT

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MFSKSMTIEQVDPDLWRAIQGEVQRQEDHIELIASENYASPAVLQAQGTVLTNKYAEGYPGKRYYGGCKHVDIVEQLAID
RLRALFNAEYVNVQPHSGSQANAAVYLSALKPGDTLLGMSLAHGGHLTHGAPVNMSGKIFNSIAYGLDPETEEIDYTELE
QLAHEHKPRMIVAGASSYARVIDWQAFRKIADNVGAYLFVDMAHYAGLIAAGYYPNPVGIADFVTSSTHKTLRGPRGGVI
MAKPEHEKALNSAVFPQTQGGPLMHVIAAKAVAFKEAASPAFKDYQKQVIENARVMARVLQQRGLRIVSGHTDCHMFLVD
LRAKNLTGREAETALETAHITVNKNAIPNDPQKPFVTSGVRIGTPAITTRGFKELESEQLANLVADVLEAPTNEAVLDQV
AREAQALCAKFPVYGN

Sequences:

>Translated_416_residues
MFSKSMTIEQVDPDLWRAIQGEVQRQEDHIELIASENYASPAVLQAQGTVLTNKYAEGYPGKRYYGGCKHVDIVEQLAID
RLRALFNAEYVNVQPHSGSQANAAVYLSALKPGDTLLGMSLAHGGHLTHGAPVNMSGKIFNSIAYGLDPETEEIDYTELE
QLAHEHKPRMIVAGASSYARVIDWQAFRKIADNVGAYLFVDMAHYAGLIAAGYYPNPVGIADFVTSSTHKTLRGPRGGVI
MAKPEHEKALNSAVFPQTQGGPLMHVIAAKAVAFKEAASPAFKDYQKQVIENARVMARVLQQRGLRIVSGHTDCHMFLVD
LRAKNLTGREAETALETAHITVNKNAIPNDPQKPFVTSGVRIGTPAITTRGFKELESEQLANLVADVLEAPTNEAVLDQV
AREAQALCAKFPVYGN
>Mature_416_residues
MFSKSMTIEQVDPDLWRAIQGEVQRQEDHIELIASENYASPAVLQAQGTVLTNKYAEGYPGKRYYGGCKHVDIVEQLAID
RLRALFNAEYVNVQPHSGSQANAAVYLSALKPGDTLLGMSLAHGGHLTHGAPVNMSGKIFNSIAYGLDPETEEIDYTELE
QLAHEHKPRMIVAGASSYARVIDWQAFRKIADNVGAYLFVDMAHYAGLIAAGYYPNPVGIADFVTSSTHKTLRGPRGGVI
MAKPEHEKALNSAVFPQTQGGPLMHVIAAKAVAFKEAASPAFKDYQKQVIENARVMARVLQQRGLRIVSGHTDCHMFLVD
LRAKNLTGREAETALETAHITVNKNAIPNDPQKPFVTSGVRIGTPAITTRGFKELESEQLANLVADVLEAPTNEAVLDQV
AREAQALCAKFPVYGN

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI19923315, Length=402, Percent_Identity=45.5223880597015, Blast_Score=346, Evalue=2e-95,
Organism=Homo sapiens, GI261862352, Length=402, Percent_Identity=45.5223880597015, Blast_Score=345, Evalue=3e-95,
Organism=Homo sapiens, GI261862350, Length=402, Percent_Identity=45.5223880597015, Blast_Score=345, Evalue=3e-95,
Organism=Homo sapiens, GI261862348, Length=402, Percent_Identity=45.5223880597015, Blast_Score=345, Evalue=3e-95,
Organism=Homo sapiens, GI261862346, Length=402, Percent_Identity=44.2786069651741, Blast_Score=327, Evalue=1e-89,
Organism=Homo sapiens, GI22547186, Length=401, Percent_Identity=42.8927680798005, Blast_Score=305, Evalue=5e-83,
Organism=Homo sapiens, GI22547189, Length=386, Percent_Identity=40.9326424870466, Blast_Score=276, Evalue=2e-74,
Organism=Escherichia coli, GI1788902, Length=417, Percent_Identity=63.5491606714628, Blast_Score=543, Evalue=1e-156,
Organism=Caenorhabditis elegans, GI25144732, Length=400, Percent_Identity=43.5, Blast_Score=333, Evalue=1e-91,
Organism=Caenorhabditis elegans, GI25144729, Length=400, Percent_Identity=43.5, Blast_Score=332, Evalue=3e-91,
Organism=Saccharomyces cerevisiae, GI6323087, Length=403, Percent_Identity=44.6650124069479, Blast_Score=332, Evalue=5e-92,
Organism=Saccharomyces cerevisiae, GI6319739, Length=436, Percent_Identity=41.2844036697248, Blast_Score=328, Evalue=9e-91,
Organism=Drosophila melanogaster, GI24640005, Length=413, Percent_Identity=45.7627118644068, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI221329721, Length=413, Percent_Identity=45.7627118644068, Blast_Score=363, Evalue=1e-100,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_NITEC (Q0AFT6)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_747761.1
- ProteinModelPortal:   Q0AFT6
- SMR:   Q0AFT6
- STRING:   Q0AFT6
- GeneID:   4273633
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_1552
- eggNOG:   COG0112
- HOGENOM:   HBG301263
- OMA:   FPQTQGG
- PhylomeDB:   Q0AFT6
- ProtClustDB:   PRK00011
- BioCyc:   NEUT335283:NEUT_1552-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 45346; Mature: 45346

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSKSMTIEQVDPDLWRAIQGEVQRQEDHIELIASENYASPAVLQAQGTVLTNKYAEGYP
CCCCCCCHHHCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEECCCEEECCCCCCCC
GKRYYGGCKHVDIVEQLAIDRLRALFNAEYVNVQPHSGSQANAAVYLSALKPGDTLLGMS
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEEEECCCCCCEEEEH
LAHGGHLTHGAPVNMSGKIFNSIAYGLDPETEEIDYTELEQLAHEHKPRMIVAGASSYAR
HHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCHHHH
VIDWQAFRKIADNVGAYLFVDMAHYAGLIAAGYYPNPVGIADFVTSSTHKTLRGPRGGVI
HHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHCCCCCCEE
MAKPEHEKALNSAVFPQTQGGPLMHVIAAKAVAFKEAASPAFKDYQKQVIENARVMARVL
EECCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
QQRGLRIVSGHTDCHMFLVDLRAKNLTGREAETALETAHITVNKNAIPNDPQKPFVTSGV
HHCCCEEEECCCCEEEEEEEHHHCCCCCCHHHHHHHEEEEEEECCCCCCCCCCCCEECCE
RIGTPAITTRGFKELESEQLANLVADVLEAPTNEAVLDQVAREAQALCAKFPVYGN
EECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MFSKSMTIEQVDPDLWRAIQGEVQRQEDHIELIASENYASPAVLQAQGTVLTNKYAEGYP
CCCCCCCHHHCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEECCCEEECCCCCCCC
GKRYYGGCKHVDIVEQLAIDRLRALFNAEYVNVQPHSGSQANAAVYLSALKPGDTLLGMS
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEEEECCCCCCEEEEH
LAHGGHLTHGAPVNMSGKIFNSIAYGLDPETEEIDYTELEQLAHEHKPRMIVAGASSYAR
HHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCHHHH
VIDWQAFRKIADNVGAYLFVDMAHYAGLIAAGYYPNPVGIADFVTSSTHKTLRGPRGGVI
HHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHCCCCCCEE
MAKPEHEKALNSAVFPQTQGGPLMHVIAAKAVAFKEAASPAFKDYQKQVIENARVMARVL
EECCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
QQRGLRIVSGHTDCHMFLVDLRAKNLTGREAETALETAHITVNKNAIPNDPQKPFVTSGV
HHCCCEEEECCCCEEEEEEEHHHCCCCCCHHHHHHHEEEEEEECCCCCCCCCCCCEECCE
RIGTPAITTRGFKELESEQLANLVADVLEAPTNEAVLDQVAREAQALCAKFPVYGN
EECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA