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The map label for this gene is yfhM [H]

Identifier: 113476297

GI number: 113476297

Start: 4170843

End: 4171718

Strand: Reverse

Name: yfhM [H]

Synonym: Tery_2696

Alternate gene names: 113476297

Gene position: 4171718-4170843 (Counterclockwise)

Preceding gene: 113476298

Following gene: 113476295

Centisome position: 53.83

GC content: 36.64

Gene sequence:

>876_bases
ATGAATATTCTAGAATCTCAATGGAAACATTTCTTTATAGACACTAATGATATTCGCCTGCATTGTGTGACTCAAGGTGA
GGGAGAATTAGTTATACTCCTTCATGGCTTCCCAGAATTCTGGTATTCTTGGCGTTATCAAATGCCTGCATTAGCACGTC
ATTTTAAGGTTGTAGTTCCAGATTTACGTGGCTACAATGATTCAGATAAACCAGATAATGGTTATGACTTAGATACTTTA
GCTGCTGATATTCGGGGACTAATTGAGCGTTGCGGTTATGTTAAGGCGCATATTGTTGGCCATGATTGGGGAGGAGCGAT
CGCATGGCACCTAGCTCAAAAATTTCCTCAACATTTAAACCGTCTAGCAATTTTAAATGCAGCTCATCCACAAAAATTCG
TTCAAGAACTGCTAGGAAATTTAGATCAATTACGCAGAAGTTGGTATATATTAGCTTTTCAAGTTCCTGGAATTCCTGAA
TGGGTAATTCAGCAAAACTTAGGAGAATTTATCAAAGGACTTTTCCAAGGACAGGCAATTCGTAAAAGTGCTTTTACTGC
TGAAATTACTCAGATTTATCAGGCAGCTTTAGAGAAACCAGGAGCTTTAGTAGCTGCAATTAATCACTATCGTCAATTTC
TTTGGCCTCAAAACTGGCTAAAAAACTTTAATAAGCCACAACTAGTTACATCACCTACTTTAATTTTATGGGGTAAAGAA
GATTATTTTTTAAGCCAAAAACTTACAGAAGGTTTAGATAAATTAATTGCTGCTCCTTTCCAGTTAAAAGTTATTCCTGA
CTGTGGTCATTGGATTCAGCAAGAAGTTCCCCACACAGTAAATAGGGAGTTGCTGAATTTTTTGAGAAAAAAATAA

Upstream 100 bases:

>100_bases
GTACAAAAAAACAAGTTAAATTTTTAGCATTAGCTGACAACAAATTACTACTAATTGATTTTGAAGAAGTTTGGCAAGAT
AGGAGAGTTGATAACAAACT

Downstream 100 bases:

>100_bases
TCATTATTTAGGTAAATTATTTAATTGTGCCTATTGTTCTACCCTATGCAAAATTGTTTATAATTAGAAGTTCAATTAAG
GCAATGATGAAACACTTAGG

Product: alpha/beta hydrolase fold protein

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MNILESQWKHFFIDTNDIRLHCVTQGEGELVILLHGFPEFWYSWRYQMPALARHFKVVVPDLRGYNDSDKPDNGYDLDTL
AADIRGLIERCGYVKAHIVGHDWGGAIAWHLAQKFPQHLNRLAILNAAHPQKFVQELLGNLDQLRRSWYILAFQVPGIPE
WVIQQNLGEFIKGLFQGQAIRKSAFTAEITQIYQAALEKPGALVAAINHYRQFLWPQNWLKNFNKPQLVTSPTLILWGKE
DYFLSQKLTEGLDKLIAAPFQLKVIPDCGHWIQQEVPHTVNRELLNFLRKK

Sequences:

>Translated_291_residues
MNILESQWKHFFIDTNDIRLHCVTQGEGELVILLHGFPEFWYSWRYQMPALARHFKVVVPDLRGYNDSDKPDNGYDLDTL
AADIRGLIERCGYVKAHIVGHDWGGAIAWHLAQKFPQHLNRLAILNAAHPQKFVQELLGNLDQLRRSWYILAFQVPGIPE
WVIQQNLGEFIKGLFQGQAIRKSAFTAEITQIYQAALEKPGALVAAINHYRQFLWPQNWLKNFNKPQLVTSPTLILWGKE
DYFLSQKLTEGLDKLIAAPFQLKVIPDCGHWIQQEVPHTVNRELLNFLRKK
>Mature_291_residues
MNILESQWKHFFIDTNDIRLHCVTQGEGELVILLHGFPEFWYSWRYQMPALARHFKVVVPDLRGYNDSDKPDNGYDLDTL
AADIRGLIERCGYVKAHIVGHDWGGAIAWHLAQKFPQHLNRLAILNAAHPQKFVQELLGNLDQLRRSWYILAFQVPGIPE
WVIQQNLGEFIKGLFQGQAIRKSAFTAEITQIYQAALEKPGALVAAINHYRQFLWPQNWLKNFNKPQLVTSPTLILWGKE
DYFLSQKLTEGLDKLIAAPFQLKVIPDCGHWIQQEVPHTVNRELLNFLRKK

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Epoxide hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI218777837, Length=287, Percent_Identity=37.2822299651568, Blast_Score=201, Evalue=8e-52,
Organism=Homo sapiens, GI218777840, Length=283, Percent_Identity=32.8621908127208, Blast_Score=175, Evalue=4e-44,
Organism=Homo sapiens, GI13376164, Length=283, Percent_Identity=32.8621908127208, Blast_Score=175, Evalue=4e-44,
Organism=Homo sapiens, GI27597073, Length=315, Percent_Identity=29.2063492063492, Blast_Score=138, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17554012, Length=303, Percent_Identity=33.993399339934, Blast_Score=179, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17562298, Length=296, Percent_Identity=33.445945945946, Blast_Score=178, Evalue=3e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 33713; Mature: 33713

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILESQWKHFFIDTNDIRLHCVTQGEGELVILLHGFPEFWYSWRYQMPALARHFKVVVP
CCCCHHCCCEEEEECCCEEEEEEECCCCCEEEEEECCHHHHHHHCCCCHHHHHHHHEEEC
DLRGYNDSDKPDNGYDLDTLAADIRGLIERCGYVKAHIVGHDWGGAIAWHLAQKFPQHLN
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHH
RLAILNAAHPQKFVQELLGNLDQLRRSWYILAFQVPGIPEWVIQQNLGEFIKGLFQGQAI
HHHHHCCCCHHHHHHHHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCHHH
RKSAFTAEITQIYQAALEKPGALVAAINHYRQFLWPQNWLKNFNKPQLVTSPTLILWGKE
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHCCCCCCEEECCEEEEECCC
DYFLSQKLTEGLDKLIAAPFQLKVIPDCGHWIQQEVPHTVNRELLNFLRKK
HHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MNILESQWKHFFIDTNDIRLHCVTQGEGELVILLHGFPEFWYSWRYQMPALARHFKVVVP
CCCCHHCCCEEEEECCCEEEEEEECCCCCEEEEEECCHHHHHHHCCCCHHHHHHHHEEEC
DLRGYNDSDKPDNGYDLDTLAADIRGLIERCGYVKAHIVGHDWGGAIAWHLAQKFPQHLN
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHH
RLAILNAAHPQKFVQELLGNLDQLRRSWYILAFQVPGIPEWVIQQNLGEFIKGLFQGQAI
HHHHHCCCCHHHHHHHHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCHHH
RKSAFTAEITQIYQAALEKPGALVAAINHYRQFLWPQNWLKNFNKPQLVTSPTLILWGKE
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHCCCCCCEEECCEEEEECCC
DYFLSQKLTEGLDKLIAAPFQLKVIPDCGHWIQQEVPHTVNRELLNFLRKK
HHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8946165; 9384377 [H]