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The map label for this gene is ligA

Identifier: 113475951

GI number: 113475951

Start: 3587593

End: 3589917

Strand: Reverse

Name: ligA

Synonym: Tery_2316

Alternate gene names: 113475951

Gene position: 3589917-3587593 (Counterclockwise)

Preceding gene: 113475952

Following gene: 113475950

Centisome position: 46.32

GC content: 37.89

Gene sequence:

>2325_bases
GTGGCAAAATTAACACTAGAAGTTGAGCAATACACTCAAAACTTAAGGGAAAAACTTCAAGAAGCTGGTTATTCTTACTA
TGTTCTTGATCAACCAATTATGGAAGATGCTGTTTATGATCACCTACTTCGAGAGTTACAGGAATTAGAAAGTCAATATC
CTCAATTAATTGTTTCAGATAGTCCTACTCAAAGAGTGGGAGAAAAGCCAGCAACTAAATTTGTATCAGTTAAACATAAT
ATTCCCCTTTATAGTCTGGAAAATGCTTTTAATATTCAGGAATTAAAAAGTTGGCAAGAACGTTGGCAGCGTCAAACACT
TCTTGAAAATCAAAGGTTAACTCTCCCCCTAAGGGGGGAAGTCAAAATTAAAAACTCTATTGATGAGTCAGATAATTTCT
CTACAGTTAATTCTTTAGCTCCAGTAATTACTGGTTCTAATTTTGATGAAATAGATTTAGCAAGTGTAGATTATATTTGT
GAATTAAAAATTGATGGTTCTGCTTTGGCTTTAACTTATGAAAATGGTTTATTAGTACGAGGTGCTACTCGCGGTGATGG
AATTATGGGGGAAGATATTACTCAAAATGTTAAGACAATTAATAGTATTCCCTTAAAACTTAAAGTTGATAATCCACCTT
TATTAGTAGAAGTTAGGGGAGAAGCTTTTTTATCAATAAATGTATTTGAAAATATTAATGCTGAAAGAGAAAAAATTGGA
GAGATTATATTTGCAAATCCACGGAATGCAGCAGCAGGTACTTTAAGACAATTAGACTCAAAAATAGTAGCCAAACGTCG
CCTAGATTTTTTTGCTTATACACTTTATTTAGAAGAAAATGAATCGAATTTTTTGCAGTTTTATACACAGTCGCAATGTT
TAGAGTTACTTAAAAAATTAGGTTTTAAAGTTAATCCTAACGGTCAAGTTTGTAGCTCTTTAAATGAAGTTGAAGCATAT
TATCAGTACTGGGATAAACAACGAGAAAATCTACCTTATTTAACTGATGGAGTAGTTGTTAAACTTAATTCTTTATCACT
ACAAGAGAAGTTAGGATTTACTCAAAAGTACCCTCGGTGGGCGATCGCTCTCAAATACCCTGCCGAAGAAATTCCAACCA
TTGTCCAAGGAGTTACAGTTCAAGTAGGGCGTACTGGGGCCATAACTCCTGTGGCCGAATTAAAACCAGTTCAGTTGGCA
GGGACAACTGTACAAAGGGCATCTCTACATAATAGCGATCGCTTGGCAGAATTGGACTTACACATTGGGGATACAGTAAT
AGTCCGCAAGGCAGGGGAAATTATTCCAGAGGTGGTCAGAGTTTTACCAGAATTACGACCAAAAAAAGCTCAAAGATTTC
AAATGCCCACTCATTGTCCAGAATGTAGTCAACCTCTAATTAAACCTAGAAATGAGGCGGTAACTCGATGTATTAATACT
TCATGTCCCGCTATTCTTCGGGGATCATTAGCACACTGGGCATCTAGGGCAGCTTTGGATATTAATGGTTTGGGAGAAAA
GTTGGTGCAGCAGTTGGTTAATAGTGGTTTGGTTAAGTCTGTGGCAGATTTGTATAATTTGACTATGGAAGATTTGACTT
CCCTGGAACGCATGGGGAAAAAGTCGGCGAATAAGTTGATAGAAGCTATGGGGACTTCAAAAGCTCAACCCTGGAGGCGG
GTGCTTTATGGTTTGGGTATTCGTCATGTAGGTACGGTGAATGCAGAGTTGTTGACTCAAAAATTTCCGACTGTGGCACA
GTTAAGCCAAGCAAAGGCAACTGACATTGAAACAGTGCATGGTATTGGTCCTGAAATTGCTCAATATGTGGAACAGTGGT
TTCGGGTACCAGCAAATCAGAATTTAGTTGAACGTCTAAAAATTGCTGGAGTGACAATGGAGACCAAAGACTCTACAAGT
GGTATGGCAAAGTTGACGGAAACTGTGCTAGCTATTCAGAATTTAGCTGCACGTCAGAATTTAGTTGCACGTCAGAATTT
AGAGAATTTAGTTGAACGTCTAAAAATTGCTGGAGTGACAATGGAGACCAAAGACTCTACAAGTGATGTGGTAGAAGTGC
AAAGTGGTTTGTTAAGAGGTAAAACTTTTGTGTTGACGGGAACTTTGCCAACTATGAGGCGAGATGAGGCAAAAAACTTG
ATTCAAAATGCTGGTGGTAAGGTTACAAGTTCGGTAAGTTCTAAGACTGACTTTATTGTAGTGGGAGAAGATGGAGGCTC
TAAGTTAGAGAAGGCAAAAAAATTAGGGGTGAAAGAGTTGAGTGAGTCAGAGTTATTGGAAGCGTTGGCAGTGACTGGGA
TATAG

Upstream 100 bases:

>100_bases
AATTTAATAGTTAAATAACTGGAATTTACTGTATATTTTCCCCTATGATTTTAAGCTAGGTTCTATCTACTCGAAAATTG
AGGCAAGGAAATAATAGATT

Downstream 100 bases:

>100_bases
TATTATATCTAGTTAATAAGGAAGGTAAAAGCTAGAAGGGAAGAAATAGAAAACTCAGTTATTTGAGCTTTTAAATCTTC
AAAATAGGTAAACATTTAGC

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 774; Mature: 773

Protein sequence:

>774_residues
MAKLTLEVEQYTQNLREKLQEAGYSYYVLDQPIMEDAVYDHLLRELQELESQYPQLIVSDSPTQRVGEKPATKFVSVKHN
IPLYSLENAFNIQELKSWQERWQRQTLLENQRLTLPLRGEVKIKNSIDESDNFSTVNSLAPVITGSNFDEIDLASVDYIC
ELKIDGSALALTYENGLLVRGATRGDGIMGEDITQNVKTINSIPLKLKVDNPPLLVEVRGEAFLSINVFENINAEREKIG
EIIFANPRNAAAGTLRQLDSKIVAKRRLDFFAYTLYLEENESNFLQFYTQSQCLELLKKLGFKVNPNGQVCSSLNEVEAY
YQYWDKQRENLPYLTDGVVVKLNSLSLQEKLGFTQKYPRWAIALKYPAEEIPTIVQGVTVQVGRTGAITPVAELKPVQLA
GTTVQRASLHNSDRLAELDLHIGDTVIVRKAGEIIPEVVRVLPELRPKKAQRFQMPTHCPECSQPLIKPRNEAVTRCINT
SCPAILRGSLAHWASRAALDINGLGEKLVQQLVNSGLVKSVADLYNLTMEDLTSLERMGKKSANKLIEAMGTSKAQPWRR
VLYGLGIRHVGTVNAELLTQKFPTVAQLSQAKATDIETVHGIGPEIAQYVEQWFRVPANQNLVERLKIAGVTMETKDSTS
GMAKLTETVLAIQNLAARQNLVARQNLENLVERLKIAGVTMETKDSTSDVVEVQSGLLRGKTFVLTGTLPTMRRDEAKNL
IQNAGGKVTSSVSSKTDFIVVGEDGGSKLEKAKKLGVKELSESELLEALAVTGI

Sequences:

>Translated_774_residues
MAKLTLEVEQYTQNLREKLQEAGYSYYVLDQPIMEDAVYDHLLRELQELESQYPQLIVSDSPTQRVGEKPATKFVSVKHN
IPLYSLENAFNIQELKSWQERWQRQTLLENQRLTLPLRGEVKIKNSIDESDNFSTVNSLAPVITGSNFDEIDLASVDYIC
ELKIDGSALALTYENGLLVRGATRGDGIMGEDITQNVKTINSIPLKLKVDNPPLLVEVRGEAFLSINVFENINAEREKIG
EIIFANPRNAAAGTLRQLDSKIVAKRRLDFFAYTLYLEENESNFLQFYTQSQCLELLKKLGFKVNPNGQVCSSLNEVEAY
YQYWDKQRENLPYLTDGVVVKLNSLSLQEKLGFTQKYPRWAIALKYPAEEIPTIVQGVTVQVGRTGAITPVAELKPVQLA
GTTVQRASLHNSDRLAELDLHIGDTVIVRKAGEIIPEVVRVLPELRPKKAQRFQMPTHCPECSQPLIKPRNEAVTRCINT
SCPAILRGSLAHWASRAALDINGLGEKLVQQLVNSGLVKSVADLYNLTMEDLTSLERMGKKSANKLIEAMGTSKAQPWRR
VLYGLGIRHVGTVNAELLTQKFPTVAQLSQAKATDIETVHGIGPEIAQYVEQWFRVPANQNLVERLKIAGVTMETKDSTS
GMAKLTETVLAIQNLAARQNLVARQNLENLVERLKIAGVTMETKDSTSDVVEVQSGLLRGKTFVLTGTLPTMRRDEAKNL
IQNAGGKVTSSVSSKTDFIVVGEDGGSKLEKAKKLGVKELSESELLEALAVTGI
>Mature_773_residues
AKLTLEVEQYTQNLREKLQEAGYSYYVLDQPIMEDAVYDHLLRELQELESQYPQLIVSDSPTQRVGEKPATKFVSVKHNI
PLYSLENAFNIQELKSWQERWQRQTLLENQRLTLPLRGEVKIKNSIDESDNFSTVNSLAPVITGSNFDEIDLASVDYICE
LKIDGSALALTYENGLLVRGATRGDGIMGEDITQNVKTINSIPLKLKVDNPPLLVEVRGEAFLSINVFENINAEREKIGE
IIFANPRNAAAGTLRQLDSKIVAKRRLDFFAYTLYLEENESNFLQFYTQSQCLELLKKLGFKVNPNGQVCSSLNEVEAYY
QYWDKQRENLPYLTDGVVVKLNSLSLQEKLGFTQKYPRWAIALKYPAEEIPTIVQGVTVQVGRTGAITPVAELKPVQLAG
TTVQRASLHNSDRLAELDLHIGDTVIVRKAGEIIPEVVRVLPELRPKKAQRFQMPTHCPECSQPLIKPRNEAVTRCINTS
CPAILRGSLAHWASRAALDINGLGEKLVQQLVNSGLVKSVADLYNLTMEDLTSLERMGKKSANKLIEAMGTSKAQPWRRV
LYGLGIRHVGTVNAELLTQKFPTVAQLSQAKATDIETVHGIGPEIAQYVEQWFRVPANQNLVERLKIAGVTMETKDSTSG
MAKLTETVLAIQNLAARQNLVARQNLENLVERLKIAGVTMETKDSTSDVVEVQSGLLRGKTFVLTGTLPTMRRDEAKNLI
QNAGGKVTSSVSSKTDFIVVGEDGGSKLEKAKKLGVKELSESELLEALAVTGI

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Homo sapiens, GI32528306, Length=70, Percent_Identity=52.8571428571429, Blast_Score=68, Evalue=3e-11,
Organism=Escherichia coli, GI1788750, Length=771, Percent_Identity=39.4293125810636, Blast_Score=523, Evalue=1e-149,
Organism=Escherichia coli, GI87082305, Length=416, Percent_Identity=27.1634615384615, Blast_Score=104, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_TRIEI (Q112N5)

Other databases:

- EMBL:   CP000393
- RefSeq:   YP_722012.1
- ProteinModelPortal:   Q112N5
- STRING:   Q112N5
- GeneID:   4245198
- GenomeReviews:   CP000393_GR
- KEGG:   ter:Tery_2316
- NMPDR:   fig|203124.1.peg.4802
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- PhylomeDB:   Q112N5
- ProtClustDB:   PRK07956
- BioCyc:   TERY203124:TERY_2316-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 86088; Mature: 85957

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 163-163 BINDING 161-161 BINDING 184-184 BINDING 221-221 BINDING 341-341 BINDING 365-365

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKLTLEVEQYTQNLREKLQEAGYSYYVLDQPIMEDAVYDHLLRELQELESQYPQLIVSD
CCEEEEEHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCEEEECC
SPTQRVGEKPATKFVSVKHNIPLYSLENAFNIQELKSWQERWQRQTLLENQRLTLPLRGE
CCHHHHCCCCCHHEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCC
VKIKNSIDESDNFSTVNSLAPVITGSNFDEIDLASVDYICELKIDGSALALTYENGLLVR
EEECCCCCCCCCCHHHHHHHHHEECCCCCCCEECCCCEEEEEEECCCEEEEEECCCEEEE
GATRGDGIMGEDITQNVKTINSIPLKLKVDNPPLLVEVRGEAFLSINVFENINAEREKIG
ECCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCEEEEECCCEEEEEEEHHCCCHHHHHHC
EIIFANPRNAAAGTLRQLDSKIVAKRRLDFFAYTLYLEENESNFLQFYTQSQCLELLKKL
CEEEECCCCHHHHHHHHHHHHHHHHHHHCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHC
GFKVNPNGQVCSSLNEVEAYYQYWDKQRENLPYLTDGVVVKLNSLSLQEKLGFTQKYPRW
CCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEECCCEEEEEECCCHHHHCCCCCCCCCE
AIALKYPAEEIPTIVQGVTVQVGRTGAITPVAELKPVQLAGTTVQRASLHNSDRLAELDL
EEEEECCHHHHHHHHCCCEEEECCCCCCCCHHHCCCEEEECCHHHHHHCCCCCCEEEEEE
HIGDTVIVRKAGEIIPEVVRVLPELRPKKAQRFQMPTHCPECSQPLIKPRNEAVTRCINT
ECCCEEEEHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCC
SCPAILRGSLAHWASRAALDINGLGEKLVQQLVNSGLVKSVADLYNLTMEDLTSLERMGK
CCHHHHHHHHHHHHHHCEEEHHHHHHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHHHHH
KSANKLIEAMGTSKAQPWRRVLYGLGIRHVGTVNAELLTQKFPTVAQLSQAKATDIETVH
HHHHHHHHHHCCCCCCHHHHHHHHCCCHHCCCCCHHHHHHHCCHHHHHHHHCCCCCHHHH
GIGPEIAQYVEQWFRVPANQNLVERLKIAGVTMETKDSTSGMAKLTETVLAIQNLAARQN
CCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHHHHHHHH
LVARQNLENLVERLKIAGVTMETKDSTSDVVEVQSGLLRGKTFVLTGTLPTMRRDEAKNL
HHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHH
IQNAGGKVTSSVSSKTDFIVVGEDGGSKLEKAKKLGVKELSESELLEALAVTGI
HHHCCCCEEECCCCCCCEEEEECCCCHHHHHHHHCCHHHCCHHHHHHHHHHCCC
>Mature Secondary Structure 
AKLTLEVEQYTQNLREKLQEAGYSYYVLDQPIMEDAVYDHLLRELQELESQYPQLIVSD
CEEEEEHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCEEEECC
SPTQRVGEKPATKFVSVKHNIPLYSLENAFNIQELKSWQERWQRQTLLENQRLTLPLRGE
CCHHHHCCCCCHHEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCC
VKIKNSIDESDNFSTVNSLAPVITGSNFDEIDLASVDYICELKIDGSALALTYENGLLVR
EEECCCCCCCCCCHHHHHHHHHEECCCCCCCEECCCCEEEEEEECCCEEEEEECCCEEEE
GATRGDGIMGEDITQNVKTINSIPLKLKVDNPPLLVEVRGEAFLSINVFENINAEREKIG
ECCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCEEEEECCCEEEEEEEHHCCCHHHHHHC
EIIFANPRNAAAGTLRQLDSKIVAKRRLDFFAYTLYLEENESNFLQFYTQSQCLELLKKL
CEEEECCCCHHHHHHHHHHHHHHHHHHHCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHC
GFKVNPNGQVCSSLNEVEAYYQYWDKQRENLPYLTDGVVVKLNSLSLQEKLGFTQKYPRW
CCEECCCCHHHHHHHHHHHHHHHHHHHHCCCCEECCCEEEEEECCCHHHHCCCCCCCCCE
AIALKYPAEEIPTIVQGVTVQVGRTGAITPVAELKPVQLAGTTVQRASLHNSDRLAELDL
EEEEECCHHHHHHHHCCCEEEECCCCCCCCHHHCCCEEEECCHHHHHHCCCCCCEEEEEE
HIGDTVIVRKAGEIIPEVVRVLPELRPKKAQRFQMPTHCPECSQPLIKPRNEAVTRCINT
ECCCEEEEHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCC
SCPAILRGSLAHWASRAALDINGLGEKLVQQLVNSGLVKSVADLYNLTMEDLTSLERMGK
CCHHHHHHHHHHHHHHCEEEHHHHHHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHHHHH
KSANKLIEAMGTSKAQPWRRVLYGLGIRHVGTVNAELLTQKFPTVAQLSQAKATDIETVH
HHHHHHHHHHCCCCCCHHHHHHHHCCCHHCCCCCHHHHHHHCCHHHHHHHHCCCCCHHHH
GIGPEIAQYVEQWFRVPANQNLVERLKIAGVTMETKDSTSGMAKLTETVLAIQNLAARQN
CCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHHHHHHHH
LVARQNLENLVERLKIAGVTMETKDSTSDVVEVQSGLLRGKTFVLTGTLPTMRRDEAKNL
HHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHH
IQNAGGKVTSSVSSKTDFIVVGEDGGSKLEKAKKLGVKELSESELLEALAVTGI
HHHCCCCEEECCCCCCCEEEEECCCCHHHHHHHHCCHHHCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA