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The map label for this gene is prfB [H]

Identifier: 113475767

GI number: 113475767

Start: 3312612

End: 3313478

Strand: Reverse

Name: prfB [H]

Synonym: Tery_2120

Alternate gene names: 113475767

Gene position: 3313478-3312612 (Counterclockwise)

Preceding gene: 113475768

Following gene: 113475766

Centisome position: 42.75

GC content: 40.14

Gene sequence:

>867_bases
TTGGAACTAGAAACAGATGAATCTTTACTAGCAGAAGCTAATTTAACAATTGCTAATCTCAACCAGGAGTTAGACCAATG
GGAGTTGCAGCAATTATTATCTGGTCTATATGACCAAAATGGAGCAGTGGTGACTATTAATGCTGGGGCAGGAGGTACAG
ATGCTCAAGATTGGGCAGAAATGTTGTTGCGGATGTATACTCGCTGGGGAGAAGAGCATAAGTATAAGGTGCAGTTGGCC
GAAATTTCTGAGGGGGATGAAGCAGGTCTTAAGTCTGCAACTTTAGAAATTATTGGTCGGTATGCTTATGGTTACTTGAA
GGCGGAAAAAGGTACTCATCGCTTAGTCAGAATTTCACCTTTTAATGCTAATGGCAAACGACAAACTAGTTTTGCTGGTG
TGGAGGTGATGCCCATATTAGACCATTCTGTAGAATTGGATATTCCAGAGAAGGATCTCGAAATTACAACTTCTCGTTCT
GGGGGTAAGGGTGGTCAAAATGTGAATAAAGTAGAAACGGCGGTGCGAGTAGTTCATATTCCTACAGGTCTTTCTGTGCG
TTGTACCCAAGAGCGTAGTCAGTTACAAAATAAGGATAAAGCTTTGGCATTATTGAAAGCTAGGTTACTGATAATTGCTC
AAGAGCAACGTGCTCAAGAAATTAATGAAATTCGCGGTGATATGGTAGAAGCTGCTTGGGGTAATCAAATTCGTAACTAT
GTTTTCCATCCTTATCAAATGGTTAAAGATTTACGGACTCAGAAGGAAACTACTGCTATTAATGATGTGATGAATGGTGA
GTTAGACCAATTTATTGAGGCTTATTTACGCCAGGAAAATCAAATGGTCGAAAGTCAGTCTACTTAA

Upstream 100 bases:

>100_bases
ACAAAAGATTCTGCAACAACTGAATGATCTGAAATCCCATCTTGAGCAATATCAACAATGGCAAAATAATCTGGAAGATA
CTAGAGCGATCGTTGAGTTA

Downstream 100 bases:

>100_bases
TTTAGTGGTGTGGGTGAACTAAGCTATGAATTAAGATATTTTCAAGATTTCAAGAGTAAGAAAAACTCAGGATCTAATAC
AAAATGGGTTAAAGTAGCGA

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MELETDESLLAEANLTIANLNQELDQWELQQLLSGLYDQNGAVVTINAGAGGTDAQDWAEMLLRMYTRWGEEHKYKVQLA
EISEGDEAGLKSATLEIIGRYAYGYLKAEKGTHRLVRISPFNANGKRQTSFAGVEVMPILDHSVELDIPEKDLEITTSRS
GGKGGQNVNKVETAVRVVHIPTGLSVRCTQERSQLQNKDKALALLKARLLIIAQEQRAQEINEIRGDMVEAAWGNQIRNY
VFHPYQMVKDLRTQKETTAINDVMNGELDQFIEAYLRQENQMVESQST

Sequences:

>Translated_288_residues
MELETDESLLAEANLTIANLNQELDQWELQQLLSGLYDQNGAVVTINAGAGGTDAQDWAEMLLRMYTRWGEEHKYKVQLA
EISEGDEAGLKSATLEIIGRYAYGYLKAEKGTHRLVRISPFNANGKRQTSFAGVEVMPILDHSVELDIPEKDLEITTSRS
GGKGGQNVNKVETAVRVVHIPTGLSVRCTQERSQLQNKDKALALLKARLLIIAQEQRAQEINEIRGDMVEAAWGNQIRNY
VFHPYQMVKDLRTQKETTAINDVMNGELDQFIEAYLRQENQMVESQST
>Mature_288_residues
MELETDESLLAEANLTIANLNQELDQWELQQLLSGLYDQNGAVVTINAGAGGTDAQDWAEMLLRMYTRWGEEHKYKVQLA
EISEGDEAGLKSATLEIIGRYAYGYLKAEKGTHRLVRISPFNANGKRQTSFAGVEVMPILDHSVELDIPEKDLEITTSRS
GGKGGQNVNKVETAVRVVHIPTGLSVRCTQERSQLQNKDKALALLKARLLIIAQEQRAQEINEIRGDMVEAAWGNQIRNY
VFHPYQMVKDLRTQKETTAINDVMNGELDQFIEAYLRQENQMVESQST

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI34577120, Length=248, Percent_Identity=35.4838709677419, Blast_Score=131, Evalue=5e-31,
Organism=Homo sapiens, GI166795303, Length=272, Percent_Identity=31.9852941176471, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI166795305, Length=177, Percent_Identity=32.7683615819209, Blast_Score=93, Evalue=3e-19,
Organism=Escherichia coli, GI2367172, Length=272, Percent_Identity=44.8529411764706, Blast_Score=249, Evalue=1e-67,
Organism=Escherichia coli, GI1787462, Length=245, Percent_Identity=37.9591836734694, Blast_Score=158, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17542784, Length=250, Percent_Identity=29.2, Blast_Score=88, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6321295, Length=287, Percent_Identity=32.0557491289199, Blast_Score=111, Evalue=1e-25,
Organism=Drosophila melanogaster, GI19921226, Length=255, Percent_Identity=35.2941176470588, Blast_Score=137, Evalue=8e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 32367; Mature: 32367

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELETDESLLAEANLTIANLNQELDQWELQQLLSGLYDQNGAVVTINAGAGGTDAQDWAE
CCCCCCHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHH
MLLRMYTRWGEEHKYKVQLAEISEGDEAGLKSATLEIIGRYAYGYLKAEKGTHRLVRISP
HHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEEEC
FNANGKRQTSFAGVEVMPILDHSVELDIPEKDLEITTSRSGGKGGQNVNKVETAVRVVHI
CCCCCCCCCCCCCEEEEEECCCCEECCCCCCCCEEEECCCCCCCCCCHHHHHHHEEEEEC
PTGLSVRCTQERSQLQNKDKALALLKARLLIIAQEQRAQEINEIRGDMVEAAWGNQIRNY
CCCCEEEECHHHHHHHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHH
VFHPYQMVKDLRTQKETTAINDVMNGELDQFIEAYLRQENQMVESQST
HCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MELETDESLLAEANLTIANLNQELDQWELQQLLSGLYDQNGAVVTINAGAGGTDAQDWAE
CCCCCCHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHH
MLLRMYTRWGEEHKYKVQLAEISEGDEAGLKSATLEIIGRYAYGYLKAEKGTHRLVRISP
HHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEEEC
FNANGKRQTSFAGVEVMPILDHSVELDIPEKDLEITTSRSGGKGGQNVNKVETAVRVVHI
CCCCCCCCCCCCCEEEEEECCCCEECCCCCCCCEEEECCCCCCCCCCHHHHHHHEEEEEC
PTGLSVRCTQERSQLQNKDKALALLKARLLIIAQEQRAQEINEIRGDMVEAAWGNQIRNY
CCCCEEEECHHHHHHHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHH
VFHPYQMVKDLRTQKETTAINDVMNGELDQFIEAYLRQENQMVESQST
HCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]