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The map label for this gene is pheT [H]

Identifier: 110633034

GI number: 110633034

Start: 752784

End: 755189

Strand: Direct

Name: pheT [H]

Synonym: Meso_0677

Alternate gene names: 110633034

Gene position: 752784-755189 (Clockwise)

Preceding gene: 110633033

Following gene: 110633035

Centisome position: 17.06

GC content: 64.3

Gene sequence:

>2406_bases
ATGAAATTCACACTCTCCTGGCTCAAGGATCACCTCGAGACCGAGGCCACGCTCGACGAGATCGTTGCAACACTCACGAT
GATCGGGCTCGAGGTCGAGTCCGTCGATGACAAGGCGGCGCTGAAGCCTTTTGTGATCGCCAAGGTGCTCACCGCCCAGC
AGCATCCGGATGCCGACCGGCTGAAGGTGCTGACGGTCGACACTGGCGACGGCAAGCCCATTCAGGTGGTCTGCGGCGCA
CCGAATGCGCGTGCGGGTCTTGTCGGCGCCTTCGCTGCCCCCGGTACATATGTCCCCGGCATTGATGTCACGCTTTCGGT
GGGGAAAATCCGCGGCGTGGAAAGCCACGGCATGATGTGCTCGGAGCGCGAGCTTAAGCTTTCCGACGAGCATGACGGCA
TCATCGACTTGCCGAACGATGCGCCGGTGGGTGAATCCTTCGCCAAATGGGCAAGGCTGGACGATCCGGTGATCGAGATT
GGGCTCACGCCCAACCGGCCGGACTGCACGAGCGTCTACGGCATTGCACGCGACCTTGCCGCCGCCGGGCTGGGCAAGCT
GAAGAGCGGCACGGTCGAGCCGGTGGCGGGGAAGGGCGCTTGCCCGGTCGATGTGAAGATCCTTGCGCCGGAACTTTGCC
CCGGCTTCGCGCTCCGACTGGTGCGCGGCGTAAAGAACGGCCCGTCTCCCAAATGGCTGCAGCAGCGGCTGATAGCCATT
GGCCTCAGGCCCATCAACACGCTGGTGGACATCACCAATTACATTACCTTCGATCGTGGCCGGCCTCTGCATGTCTTCGA
TGCCGTCAAGGTGAAGGGCAATCTCGTCATTCGCCGCGCTGAGGCAGGCGAGAAGGTGTTGGCTCTCGACGGGCGCGAAT
ACGAGCTGAACCCGGAGGTCTGCGTCATTGCCGATGACAATGGCGTCGAATCCATTGCCGGCGTTATGGGCGGCGAACAT
TCCGGCTGCGACGAGAACACCACCGACGTGCTGATCGAGTCCGCCCTTTGGAACGCGCTCAATATCGCCCGCACGGGCCG
TGATCTCGGCATCATCACCGATGCGCGTTACCGCTTCGAGCGCGGTGTGGACCCGGAATTCATGGTGCCGGGGCTGGAGC
TTGCCACCCGCATGGTGATCGATCTCTGCGGCGGCGAAGCCTCCGAGGCGCGTGTCGTCGGCTACCGCGGTCATACGGCA
AAGATCGTCACCTTCCCGACGAGCGAGGTGAAGCGCCTAACGGGTCTCGATGTCCCGGCTGATGAAAGCCTCTCCATCCT
CTCCCGTCTCGGCTTCAAGCCGGAAGGTTCTGGCGATGTTGTTGAGGTCTCCGTTCCCTCCTGGCGGCCGGATATCGACG
GCAAGGCCGATCTGGTCGAAGAGGTGATGCGCATCCACGGCGTGAACAGGATCGAGCCGCAGCCAATGATCGCCAAGGAT
GCGGTCGGCGGGCGCGTCCTTACCGCAGCGCAGATCCGCACGCGCCAGGCCAAGCGTGCGCTCGCCGTGCGCGGCATGAT
GGAGGCCGTCACCTGGTCCTTCATCCCGGAAGAGCACGCGCGGCTCTTCGCGGAGAACCGGAAGCTCATCAAGCTTGCCA
ACCCGATCGCCGCTGACATGTCCACCATGCGGCCTTCGCTGATGCCGGGCCTGATCGCGGCAGCGCAGCGCAATGCCGAC
CGCGGCTTCGCCGATGTTGCCCTGTTCGAGGTGTCCGCCGCCTATGAGGGCGACCAGCCGGAGGAGCAGCGCCGCGCTGC
CGCCGGCCTGCGCCGTGGCACGGCGCGGCTTGACGCGCAGGGTCGTCATTGGTCGGGCAATACCGCGCCTGTCGACGTCT
TCGCGGCCAAGGCCGATGCTTTTGCCGTGCTCGAAGCCTGCGGCGCACCCGTGGACAAGCTGCAGGTGGAAGCGGGCGGG
CCGCACTGGTATCATCCCGGCCGGTCCGGCACGATCAAGCTCGGGCCGAAGAACATTCTCGGCACATTCGGCGAGTTCCA
CCCAAAGACGCTGGAGGAGATGGACGTGTCCGGCCCGCTCTGCGGCTTCGAGGTGTTCCTCGATGCGATCCCCGAGCCCA
AGGCGCGAGCCACCCGCACCAAGCCGCGTCTCGACCTCTCGCCCTTCCAGGCGGTGAAGCGCGACTTCGCCTTCGTGGTC
GACAAAACGGTCGAGGCGGGGACGCTCACCCGCGCGGCGGCGAGCGCCGACAAGAAGCTGGTTTCGGACGTGAATGTCTT
CGACGTTTTCGAAGGTGCGGCTCTTGGCCCGGACAAAAAGTCGATCGCAATCGAGGTGACGATCCAGCCGATCGAGCGTA
CGCTGACGGATGAGGATTTCGAGGCGCTGGCGAACCGGATTGTCGAAAATGTCCGCAAGCAGACAGGTGGCGTGCTGAGA
AGCTGA

Upstream 100 bases:

>100_bases
GACGTGCGCTGGCTCGAACATTACGGCTTCCGCCCGCTCGACCTGCCGACATTGTTCGGCGGGCTGAGCAGCTGATTTTT
TCCAGGCAGGCTGACCAAAG

Downstream 100 bases:

>100_bases
AACACCGGAAGGGAGGAAGGGCATGTTCCGTTGGGGTATTTTGTCTACGGCCAAGATCGGCCGCGAACATGTCATTCCTC
AGCTTCTGGACGCGGAGAAT

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 801; Mature: 801

Protein sequence:

>801_residues
MKFTLSWLKDHLETEATLDEIVATLTMIGLEVESVDDKAALKPFVIAKVLTAQQHPDADRLKVLTVDTGDGKPIQVVCGA
PNARAGLVGAFAAPGTYVPGIDVTLSVGKIRGVESHGMMCSERELKLSDEHDGIIDLPNDAPVGESFAKWARLDDPVIEI
GLTPNRPDCTSVYGIARDLAAAGLGKLKSGTVEPVAGKGACPVDVKILAPELCPGFALRLVRGVKNGPSPKWLQQRLIAI
GLRPINTLVDITNYITFDRGRPLHVFDAVKVKGNLVIRRAEAGEKVLALDGREYELNPEVCVIADDNGVESIAGVMGGEH
SGCDENTTDVLIESALWNALNIARTGRDLGIITDARYRFERGVDPEFMVPGLELATRMVIDLCGGEASEARVVGYRGHTA
KIVTFPTSEVKRLTGLDVPADESLSILSRLGFKPEGSGDVVEVSVPSWRPDIDGKADLVEEVMRIHGVNRIEPQPMIAKD
AVGGRVLTAAQIRTRQAKRALAVRGMMEAVTWSFIPEEHARLFAENRKLIKLANPIAADMSTMRPSLMPGLIAAAQRNAD
RGFADVALFEVSAAYEGDQPEEQRRAAAGLRRGTARLDAQGRHWSGNTAPVDVFAAKADAFAVLEACGAPVDKLQVEAGG
PHWYHPGRSGTIKLGPKNILGTFGEFHPKTLEEMDVSGPLCGFEVFLDAIPEPKARATRTKPRLDLSPFQAVKRDFAFVV
DKTVEAGTLTRAAASADKKLVSDVNVFDVFEGAALGPDKKSIAIEVTIQPIERTLTDEDFEALANRIVENVRKQTGGVLR
S

Sequences:

>Translated_801_residues
MKFTLSWLKDHLETEATLDEIVATLTMIGLEVESVDDKAALKPFVIAKVLTAQQHPDADRLKVLTVDTGDGKPIQVVCGA
PNARAGLVGAFAAPGTYVPGIDVTLSVGKIRGVESHGMMCSERELKLSDEHDGIIDLPNDAPVGESFAKWARLDDPVIEI
GLTPNRPDCTSVYGIARDLAAAGLGKLKSGTVEPVAGKGACPVDVKILAPELCPGFALRLVRGVKNGPSPKWLQQRLIAI
GLRPINTLVDITNYITFDRGRPLHVFDAVKVKGNLVIRRAEAGEKVLALDGREYELNPEVCVIADDNGVESIAGVMGGEH
SGCDENTTDVLIESALWNALNIARTGRDLGIITDARYRFERGVDPEFMVPGLELATRMVIDLCGGEASEARVVGYRGHTA
KIVTFPTSEVKRLTGLDVPADESLSILSRLGFKPEGSGDVVEVSVPSWRPDIDGKADLVEEVMRIHGVNRIEPQPMIAKD
AVGGRVLTAAQIRTRQAKRALAVRGMMEAVTWSFIPEEHARLFAENRKLIKLANPIAADMSTMRPSLMPGLIAAAQRNAD
RGFADVALFEVSAAYEGDQPEEQRRAAAGLRRGTARLDAQGRHWSGNTAPVDVFAAKADAFAVLEACGAPVDKLQVEAGG
PHWYHPGRSGTIKLGPKNILGTFGEFHPKTLEEMDVSGPLCGFEVFLDAIPEPKARATRTKPRLDLSPFQAVKRDFAFVV
DKTVEAGTLTRAAASADKKLVSDVNVFDVFEGAALGPDKKSIAIEVTIQPIERTLTDEDFEALANRIVENVRKQTGGVLR
S
>Mature_801_residues
MKFTLSWLKDHLETEATLDEIVATLTMIGLEVESVDDKAALKPFVIAKVLTAQQHPDADRLKVLTVDTGDGKPIQVVCGA
PNARAGLVGAFAAPGTYVPGIDVTLSVGKIRGVESHGMMCSERELKLSDEHDGIIDLPNDAPVGESFAKWARLDDPVIEI
GLTPNRPDCTSVYGIARDLAAAGLGKLKSGTVEPVAGKGACPVDVKILAPELCPGFALRLVRGVKNGPSPKWLQQRLIAI
GLRPINTLVDITNYITFDRGRPLHVFDAVKVKGNLVIRRAEAGEKVLALDGREYELNPEVCVIADDNGVESIAGVMGGEH
SGCDENTTDVLIESALWNALNIARTGRDLGIITDARYRFERGVDPEFMVPGLELATRMVIDLCGGEASEARVVGYRGHTA
KIVTFPTSEVKRLTGLDVPADESLSILSRLGFKPEGSGDVVEVSVPSWRPDIDGKADLVEEVMRIHGVNRIEPQPMIAKD
AVGGRVLTAAQIRTRQAKRALAVRGMMEAVTWSFIPEEHARLFAENRKLIKLANPIAADMSTMRPSLMPGLIAAAQRNAD
RGFADVALFEVSAAYEGDQPEEQRRAAAGLRRGTARLDAQGRHWSGNTAPVDVFAAKADAFAVLEACGAPVDKLQVEAGG
PHWYHPGRSGTIKLGPKNILGTFGEFHPKTLEEMDVSGPLCGFEVFLDAIPEPKARATRTKPRLDLSPFQAVKRDFAFVV
DKTVEAGTLTRAAASADKKLVSDVNVFDVFEGAALGPDKKSIAIEVTIQPIERTLTDEDFEALANRIVENVRKQTGGVLR
S

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788006, Length=812, Percent_Identity=34.4827586206897, Blast_Score=454, Evalue=1e-128,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 86248; Mature: 86248

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTLSWLKDHLETEATLDEIVATLTMIGLEVESVDDKAALKPFVIAKVLTAQQHPDADR
CCEEHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHCCCCCCCC
LKVLTVDTGDGKPIQVVCGAPNARAGLVGAFAAPGTYVPGIDVTLSVGKIRGVESHGMMC
EEEEEEECCCCCEEEEEECCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCHHCCCEE
SERELKLSDEHDGIIDLPNDAPVGESFAKWARLDDPVIEIGLTPNRPDCTSVYGIARDLA
CCCCCCCCCCCCCEEECCCCCCCCHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHH
AAGLGKLKSGTVEPVAGKGACPVDVKILAPELCPGFALRLVRGVKNGPSPKWLQQRLIAI
HHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHH
GLRPINTLVDITNYITFDRGRPLHVFDAVKVKGNLVIRRAEAGEKVLALDGREYELNPEV
CCCHHHHHHHHHHHEEECCCCCEEEEEEEEEECCEEEEECCCCCEEEEECCCEEECCCCE
CVIADDNGVESIAGVMGGEHSGCDENTTDVLIESALWNALNIARTGRDLGIITDARYRFE
EEEECCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHH
RGVDPEFMVPGLELATRMVIDLCGGEASEARVVGYRGHTAKIVTFPTSEVKRLTGLDVPA
CCCCCCEECCCHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEEECCHHHHHHHHCCCCCC
DESLSILSRLGFKPEGSGDVVEVSVPSWRPDIDGKADLVEEVMRIHGVNRIEPQPMIAKD
CHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHH
AVGGRVLTAAQIRTRQAKRALAVRGMMEAVTWSFIPEEHARLFAENRKLIKLANPIAADM
CCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEECCCHHHHH
STMRPSLMPGLIAAAQRNADRGFADVALFEVSAAYEGDQPEEQRRAAAGLRRGTARLDAQ
HHHCHHHHHHHHHHHHCCCCCCCHHEEEEEEEHHCCCCCCHHHHHHHHHHHHCCHHCCCC
GRHWSGNTAPVDVFAAKADAFAVLEACGAPVDKLQVEAGGPHWYHPGRSGTIKLGPKNIL
CCCCCCCCCCEEEEEECHHHHHHHHHHCCCHHHEEEECCCCCCCCCCCCCEEEECCHHHH
GTFGEFHPKTLEEMDVSGPLCGFEVFLDAIPEPKARATRTKPRLDLSPFQAVKRDFAFVV
HHHHCCCCCHHHHCCCCCCCHHHHHHHHCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHH
DKTVEAGTLTRAAASADKKLVSDVNVFDVFEGAALGPDKKSIAIEVTIQPIERTLTDEDF
HCCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCEEEEEEEEEHHHHHCCCHHH
EALANRIVENVRKQTGGVLRS
HHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKFTLSWLKDHLETEATLDEIVATLTMIGLEVESVDDKAALKPFVIAKVLTAQQHPDADR
CCEEHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHCCCCCCCC
LKVLTVDTGDGKPIQVVCGAPNARAGLVGAFAAPGTYVPGIDVTLSVGKIRGVESHGMMC
EEEEEEECCCCCEEEEEECCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCHHCCCEE
SERELKLSDEHDGIIDLPNDAPVGESFAKWARLDDPVIEIGLTPNRPDCTSVYGIARDLA
CCCCCCCCCCCCCEEECCCCCCCCHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHH
AAGLGKLKSGTVEPVAGKGACPVDVKILAPELCPGFALRLVRGVKNGPSPKWLQQRLIAI
HHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHH
GLRPINTLVDITNYITFDRGRPLHVFDAVKVKGNLVIRRAEAGEKVLALDGREYELNPEV
CCCHHHHHHHHHHHEEECCCCCEEEEEEEEEECCEEEEECCCCCEEEEECCCEEECCCCE
CVIADDNGVESIAGVMGGEHSGCDENTTDVLIESALWNALNIARTGRDLGIITDARYRFE
EEEECCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHH
RGVDPEFMVPGLELATRMVIDLCGGEASEARVVGYRGHTAKIVTFPTSEVKRLTGLDVPA
CCCCCCEECCCHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEEECCHHHHHHHHCCCCCC
DESLSILSRLGFKPEGSGDVVEVSVPSWRPDIDGKADLVEEVMRIHGVNRIEPQPMIAKD
CHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHH
AVGGRVLTAAQIRTRQAKRALAVRGMMEAVTWSFIPEEHARLFAENRKLIKLANPIAADM
CCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEECCCHHHHH
STMRPSLMPGLIAAAQRNADRGFADVALFEVSAAYEGDQPEEQRRAAAGLRRGTARLDAQ
HHHCHHHHHHHHHHHHCCCCCCCHHEEEEEEEHHCCCCCCHHHHHHHHHHHHCCHHCCCC
GRHWSGNTAPVDVFAAKADAFAVLEACGAPVDKLQVEAGGPHWYHPGRSGTIKLGPKNIL
CCCCCCCCCCEEEEEECHHHHHHHHHHCCCHHHEEEECCCCCCCCCCCCCEEEECCHHHH
GTFGEFHPKTLEEMDVSGPLCGFEVFLDAIPEPKARATRTKPRLDLSPFQAVKRDFAFVV
HHHHCCCCCHHHHCCCCCCCHHHHHHHHCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHH
DKTVEAGTLTRAAASADKKLVSDVNVFDVFEGAALGPDKKSIAIEVTIQPIERTLTDEDF
HCCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCEEEEEEEEEHHHHHCCCHHH
EALANRIVENVRKQTGGVLRS
HHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]