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The map label for this gene is pheS

Identifier: 110633033

GI number: 110633033

Start: 751673

End: 752758

Strand: Direct

Name: pheS

Synonym: Meso_0676

Alternate gene names: 110633033

Gene position: 751673-752758 (Clockwise)

Preceding gene: 110633032

Following gene: 110633034

Centisome position: 17.04

GC content: 61.51

Gene sequence:

>1086_bases
ATGAACGATCTGGACCAACTCGAAAAAAAGATCATGGATGAGATCGCCGAGGCAGGCGACGAGCAGACTGTGGAGGCCGT
TCGCGTTGCAGCTTTGGGCAAGAAGGGCACGGTCTCGGAGAAGCTGAAGACGCTGGGCTCCATGTCGCCCGAAGAGCGGC
AGGTTATGGGCCCCGCGATCAACGGGCTGAAGAACCGCGTTACCGAAGCGCTCGCCGCCCGCCGCGCGGAGCTGCGCGAC
ATCGCCATTGCAGCAAGGCTGGAGCGCGAGAAGGTGGATGTCACCTTGCCGATGCCCCGCCCGCCGGCGGAGCGCGGCCG
CATCCATCCTATTACGCAGGTGATTGACGAGATTGCAGCCATCTTCGGCGATCTCGGTTTTTCGATTGCCGAAGGGCCGG
ATATCGAGACGGATTATTACAACTTCACCGCGCTGAACTTCCCCGAGGGCCATCCGGCGCGGGAGATGCACGACACCTTC
TTCTTCGAGCCGGACGAGAAGGGCGAGCGCAAGCTTTTGCGCACGCACACCTCGCCGGTGCAGATTCGCACCATGGAGGC
GCAGAAGCCGCCGATCCGCATCGTGATTCCGGGCAAGACCTACCGGTCCGATTCGGATGCCACTCACACGCCCATGTTCC
ACCAGCTCGAAGGGCTGGTAATCGACAAGACGGCGAATGTCGCCAACATGAAGTGGGTGCTGACCGAATTCTGCAAAGCC
TATTTCGAGGTGCCGTCGCTCAATATGCGCTTCCGTCCGAGCTTCTTCCCCTTCACCGAACCGAGCCTGGAGGTGGACAT
CCAATGCGACCGCTCCAAGCCCGGCGAGATCCGCTTTGGTGAAGGCAATGACTGGCTGGAGATTCTGGGCTGCGGCATGG
TTCATTCCAACGTGCTGCGCCATGGCGGGCTCGATCCCGACGAGTACCAGGGTTTTGCCTGGGGCATGGGCATCGACCGC
ATCGCCATGCTGAAATATGGCATGCCCGACCTGCGCGCCTTCTTCGATGCCGACGTGCGCTGGCTCGAACATTACGGCTT
CCGCCCGCTCGACCTGCCGACATTGTTCGGCGGGCTGAGCAGCTGA

Upstream 100 bases:

>100_bases
CAACCCGATTTTTGAAGCGCGCCCTGGGAGCCACGGGCGCGCACGCCAAAGCTTTGAATAGAATGCGGCTCGGCTCTGCC
GAGGGGGTGAAGGAAGGACC

Downstream 100 bases:

>100_bases
TTTTTTCCAGGCAGGCTGACCAAAGATGAAATTCACACTCTCCTGGCTCAAGGATCACCTCGAGACCGAGGCCACGCTCG
ACGAGATCGTTGCAACACTC

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MNDLDQLEKKIMDEIAEAGDEQTVEAVRVAALGKKGTVSEKLKTLGSMSPEERQVMGPAINGLKNRVTEALAARRAELRD
IAIAARLEREKVDVTLPMPRPPAERGRIHPITQVIDEIAAIFGDLGFSIAEGPDIETDYYNFTALNFPEGHPAREMHDTF
FFEPDEKGERKLLRTHTSPVQIRTMEAQKPPIRIVIPGKTYRSDSDATHTPMFHQLEGLVIDKTANVANMKWVLTEFCKA
YFEVPSLNMRFRPSFFPFTEPSLEVDIQCDRSKPGEIRFGEGNDWLEILGCGMVHSNVLRHGGLDPDEYQGFAWGMGIDR
IAMLKYGMPDLRAFFDADVRWLEHYGFRPLDLPTLFGGLSS

Sequences:

>Translated_361_residues
MNDLDQLEKKIMDEIAEAGDEQTVEAVRVAALGKKGTVSEKLKTLGSMSPEERQVMGPAINGLKNRVTEALAARRAELRD
IAIAARLEREKVDVTLPMPRPPAERGRIHPITQVIDEIAAIFGDLGFSIAEGPDIETDYYNFTALNFPEGHPAREMHDTF
FFEPDEKGERKLLRTHTSPVQIRTMEAQKPPIRIVIPGKTYRSDSDATHTPMFHQLEGLVIDKTANVANMKWVLTEFCKA
YFEVPSLNMRFRPSFFPFTEPSLEVDIQCDRSKPGEIRFGEGNDWLEILGCGMVHSNVLRHGGLDPDEYQGFAWGMGIDR
IAMLKYGMPDLRAFFDADVRWLEHYGFRPLDLPTLFGGLSS
>Mature_361_residues
MNDLDQLEKKIMDEIAEAGDEQTVEAVRVAALGKKGTVSEKLKTLGSMSPEERQVMGPAINGLKNRVTEALAARRAELRD
IAIAARLEREKVDVTLPMPRPPAERGRIHPITQVIDEIAAIFGDLGFSIAEGPDIETDYYNFTALNFPEGHPAREMHDTF
FFEPDEKGERKLLRTHTSPVQIRTMEAQKPPIRIVIPGKTYRSDSDATHTPMFHQLEGLVIDKTANVANMKWVLTEFCKA
YFEVPSLNMRFRPSFFPFTEPSLEVDIQCDRSKPGEIRFGEGNDWLEILGCGMVHSNVLRHGGLDPDEYQGFAWGMGIDR
IAMLKYGMPDLRAFFDADVRWLEHYGFRPLDLPTLFGGLSS

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=278, Percent_Identity=29.8561151079137, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI5729820, Length=276, Percent_Identity=26.8115942028986, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1788007, Length=345, Percent_Identity=50.4347826086956, Blast_Score=352, Evalue=2e-98,
Organism=Caenorhabditis elegans, GI32563657, Length=319, Percent_Identity=28.8401253918495, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17508957, Length=319, Percent_Identity=28.8401253918495, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI32566635, Length=263, Percent_Identity=29.277566539924, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321087, Length=276, Percent_Identity=28.2608695652174, Blast_Score=117, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6325304, Length=100, Percent_Identity=35, Blast_Score=76, Evalue=8e-15,
Organism=Drosophila melanogaster, GI18858079, Length=273, Percent_Identity=30.03663003663, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI17137424, Length=304, Percent_Identity=25.6578947368421, Blast_Score=91, Evalue=8e-19,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_MESSB (Q11KJ9)

Other databases:

- EMBL:   CP000390
- RefSeq:   YP_673241.1
- STRING:   Q11KJ9
- GeneID:   4180622
- GenomeReviews:   CP000390_GR
- KEGG:   mes:Meso_0676
- NMPDR:   fig|266779.1.peg.2812
- eggNOG:   COG0016
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- PhylomeDB:   Q11KJ9
- ProtClustDB:   PRK00488
- BioCyc:   MSP266779:MESO_0676-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 40696; Mature: 40696

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDLDQLEKKIMDEIAEAGDEQTVEAVRVAALGKKGTVSEKLKTLGSMSPEERQVMGPAI
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHH
NGLKNRVTEALAARRAELRDIAIAARLEREKVDVTLPMPRPPAERGRIHPITQVIDEIAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHHHH
IFGDLGFSIAEGPDIETDYYNFTALNFPEGHPAREMHDTFFFEPDEKGERKLLRTHTSPV
HHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHEECCCCCHHHHHHHHCCCCE
QIRTMEAQKPPIRIVIPGKTYRSDSDATHTPMFHQLEGLVIDKTANVANMKWVLTEFCKA
EEEEEECCCCCEEEEECCCCCCCCCCCCCCCHHHHHCCEEEECCCCCHHHHHHHHHHHHH
YFEVPSLNMRFRPSFFPFTEPSLEVDIQCDRSKPGEIRFGEGNDWLEILGCGMVHSNVLR
HHCCCCCCEEECCCCCCCCCCCEEEEEEECCCCCCCEEECCCCCHHHHHHHHHHHHHHHH
HGGLDPDEYQGFAWGMGIDRIAMLKYGMPDLRAFFDADVRWLEHYGFRPLDLPTLFGGLS
HCCCCHHHCCCEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCC
S
C
>Mature Secondary Structure
MNDLDQLEKKIMDEIAEAGDEQTVEAVRVAALGKKGTVSEKLKTLGSMSPEERQVMGPAI
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHH
NGLKNRVTEALAARRAELRDIAIAARLEREKVDVTLPMPRPPAERGRIHPITQVIDEIAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCHHHHHHHHHHH
IFGDLGFSIAEGPDIETDYYNFTALNFPEGHPAREMHDTFFFEPDEKGERKLLRTHTSPV
HHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHEECCCCCHHHHHHHHCCCCE
QIRTMEAQKPPIRIVIPGKTYRSDSDATHTPMFHQLEGLVIDKTANVANMKWVLTEFCKA
EEEEEECCCCCEEEEECCCCCCCCCCCCCCCHHHHHCCEEEECCCCCHHHHHHHHHHHHH
YFEVPSLNMRFRPSFFPFTEPSLEVDIQCDRSKPGEIRFGEGNDWLEILGCGMVHSNVLR
HHCCCCCCEEECCCCCCCCCCCEEEEEEECCCCCCCEEECCCCCHHHHHHHHHHHHHHHH
HGGLDPDEYQGFAWGMGIDRIAMLKYGMPDLRAFFDADVRWLEHYGFRPLDLPTLFGGLS
HCCCCHHHCCCEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCC
S
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA