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The map label for this gene is gatA

Identifier: 109947725

GI number: 109947725

Start: 1035482

End: 1036843

Strand: Reverse

Name: gatA

Synonym: Hac_1198

Alternate gene names: 109947725

Gene position: 1036843-1035482 (Counterclockwise)

Preceding gene: 109947727

Following gene: 109947724

Centisome position: 66.72

GC content: 42.58

Gene sequence:

>1362_bases
ATGATCACTTTAAAACAAGCCCTTTCTTTATCCCAAGATGAATTAGAAACCCTTAAAAACGGAATTGACGCTAATGTTAG
AGCTTCAGATTTGAACGCTTACATTAAAGCCCCTAGTCTTAATGGTGCTAGTGCTAAGGGTGTGCCTATCCTTATTAAAG
ACAATATCAGCGTTAAGGGGTGGGAGATCACTTGCTCTAGTAAGATTTTAGAGGGCTATGTGGCTCCTTATCATGCGAGC
GTGATTGAAAACTTGCACCAAAACAGCATGGCAGGGTTTGGGCTTTCTAACATGGACGAGTTTGCGATGGGGAGCACCAC
AGAGTCTAGTTGCTATGGGATCACTAAAAACCCACGAGATAAAAACAGAGTGCCTGGAGGGAGTAGCGGGGGGAGTGCCG
CAGCGGTGGCAGGTGGCTTGGCTGTGGCAGCTTTAGGGAGCGATACGGGTGGGTCTATCAGACAGCCAGCGAGCTATTGT
GGGTGCGTGGGGTTAAAGCCCACTTATGGGAGGGTGAGCCGTTATGGTTTGATTGCGTATTGTTCTAGTTTTGATCAAAT
TGGGCCTATCACACAAAATGTAGAGGACGCTTCTATTTTATTTGACGCTATTAGTGGGCATGATAGTAAGGACTCTACGA
GTGCGAATCTCAAACCCACGCAAACTTTCAAAAATCTTAATAGAGAAAGACGCTTTAAAATCGCTGTTTTAAGAGATCAC
ATTAAAGATGCGAGTAATGAAGTGCAACTCGCTTATGAAAACACCCTTAAGGCTTTGAAAGAAATAGGGCATGAGATTGT
AGAAAAAAAGATATTGGATTCGCATTATCAAATCTCTATCTATTATATTATTAGCATGGCTGAAGCGAGCTCCAATCTGG
CTAGATTTGATGGGGTGCGTTATGGGAGAAGGGCTCAAAATATTAAAGATTTAAAAGAATTGTATCTTAAAAGCCGTAGT
GAAGGTTTTGGCGACGAGGTGAAACGCCGTATCATGCTAGGGAATTTTGTCTTAAGCAGTGGGTATTATGACGCTTATTA
TTTGAAAGCCCAACAAATGCGTTTGATGATCAAAGAGCAATACAACAAGATTTTTGAAGAAGTGGATTTGATTTTCACTC
CTGTGGCTCCCACGACCGCCCCCTTATTCAACTACCATGCAAGCCCTTTAGAAATGTATTTGAGCGATATTTACACGATC
GGAGCGAATTTGAGCGGTTTGCCGGCCCTTTCTTTGCCGGTCGCTAAAGATCCTTTAGGCTTGCCCATAGGGATGCAATT
CATTGCTAAGGCTTTTGATGAGCAAAGCCTTTTAGATGTTTCCTACGCTTTAGAGCAGGAATTAAAATTAAAACTAGATT
AA

Upstream 100 bases:

>100_bases
TCCGATTTTCTAAGAGACTTTCATTGTCAACCAATGCTTATAAGCTCGTTTTGTATTGAAGCATGTTAAACTATCATTAA
ATTTTTTGTAAGGCTAAAAC

Downstream 100 bases:

>100_bases
GGATAGAAAATGAGAATTTTACAAAGGGCTTTGACTTTTGAAGATGTGTTGATGGTGCCTAGAAAATCTAGCGTTTTACC
TAAAGATGTGAGTTTAAAAT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MITLKQALSLSQDELETLKNGIDANVRASDLNAYIKAPSLNGASAKGVPILIKDNISVKGWEITCSSKILEGYVAPYHAS
VIENLHQNSMAGFGLSNMDEFAMGSTTESSCYGITKNPRDKNRVPGGSSGGSAAAVAGGLAVAALGSDTGGSIRQPASYC
GCVGLKPTYGRVSRYGLIAYCSSFDQIGPITQNVEDASILFDAISGHDSKDSTSANLKPTQTFKNLNRERRFKIAVLRDH
IKDASNEVQLAYENTLKALKEIGHEIVEKKILDSHYQISIYYIISMAEASSNLARFDGVRYGRRAQNIKDLKELYLKSRS
EGFGDEVKRRIMLGNFVLSSGYYDAYYLKAQQMRLMIKEQYNKIFEEVDLIFTPVAPTTAPLFNYHASPLEMYLSDIYTI
GANLSGLPALSLPVAKDPLGLPIGMQFIAKAFDEQSLLDVSYALEQELKLKLD

Sequences:

>Translated_453_residues
MITLKQALSLSQDELETLKNGIDANVRASDLNAYIKAPSLNGASAKGVPILIKDNISVKGWEITCSSKILEGYVAPYHAS
VIENLHQNSMAGFGLSNMDEFAMGSTTESSCYGITKNPRDKNRVPGGSSGGSAAAVAGGLAVAALGSDTGGSIRQPASYC
GCVGLKPTYGRVSRYGLIAYCSSFDQIGPITQNVEDASILFDAISGHDSKDSTSANLKPTQTFKNLNRERRFKIAVLRDH
IKDASNEVQLAYENTLKALKEIGHEIVEKKILDSHYQISIYYIISMAEASSNLARFDGVRYGRRAQNIKDLKELYLKSRS
EGFGDEVKRRIMLGNFVLSSGYYDAYYLKAQQMRLMIKEQYNKIFEEVDLIFTPVAPTTAPLFNYHASPLEMYLSDIYTI
GANLSGLPALSLPVAKDPLGLPIGMQFIAKAFDEQSLLDVSYALEQELKLKLD
>Mature_453_residues
MITLKQALSLSQDELETLKNGIDANVRASDLNAYIKAPSLNGASAKGVPILIKDNISVKGWEITCSSKILEGYVAPYHAS
VIENLHQNSMAGFGLSNMDEFAMGSTTESSCYGITKNPRDKNRVPGGSSGGSAAAVAGGLAVAALGSDTGGSIRQPASYC
GCVGLKPTYGRVSRYGLIAYCSSFDQIGPITQNVEDASILFDAISGHDSKDSTSANLKPTQTFKNLNRERRFKIAVLRDH
IKDASNEVQLAYENTLKALKEIGHEIVEKKILDSHYQISIYYIISMAEASSNLARFDGVRYGRRAQNIKDLKELYLKSRS
EGFGDEVKRRIMLGNFVLSSGYYDAYYLKAQQMRLMIKEQYNKIFEEVDLIFTPVAPTTAPLFNYHASPLEMYLSDIYTI
GANLSGLPALSLPVAKDPLGLPIGMQFIAKAFDEQSLLDVSYALEQELKLKLD

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=433, Percent_Identity=36.2586605080831, Blast_Score=273, Evalue=2e-73,
Organism=Homo sapiens, GI195972892, Length=407, Percent_Identity=24.0786240786241, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17543272, Length=407, Percent_Identity=35.1351351351351, Blast_Score=237, Evalue=1e-62,
Organism=Caenorhabditis elegans, GI17537465, Length=418, Percent_Identity=26.7942583732057, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17556264, Length=251, Percent_Identity=31.0756972111554, Blast_Score=111, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI71990152, Length=264, Percent_Identity=31.0606060606061, Blast_Score=104, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17556276, Length=249, Percent_Identity=30.1204819277108, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17556278, Length=249, Percent_Identity=30.1204819277108, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17538252, Length=259, Percent_Identity=24.7104247104247, Blast_Score=83, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6323950, Length=417, Percent_Identity=35.4916067146283, Blast_Score=224, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6319685, Length=396, Percent_Identity=27.2727272727273, Blast_Score=98, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24648113, Length=472, Percent_Identity=37.0762711864407, Blast_Score=271, Evalue=8e-73,
Organism=Drosophila melanogaster, GI24644968, Length=425, Percent_Identity=26.5882352941176, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24652985, Length=417, Percent_Identity=26.6187050359712, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI19922090, Length=417, Percent_Identity=26.6187050359712, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24652981, Length=417, Percent_Identity=26.6187050359712, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24652983, Length=417, Percent_Identity=26.6187050359712, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24648435, Length=433, Percent_Identity=25.1732101616628, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24648437, Length=433, Percent_Identity=25.1732101616628, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI45550774, Length=433, Percent_Identity=25.1732101616628, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24648441, Length=418, Percent_Identity=23.9234449760766, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24648439, Length=418, Percent_Identity=23.9234449760766, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21356731, Length=419, Percent_Identity=24.3436754176611, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI161078093, Length=422, Percent_Identity=25.1184834123223, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_HELAH (Q17WM2)

Other databases:

- EMBL:   AM260522
- RefSeq:   YP_664953.1
- ProteinModelPortal:   Q17WM2
- SMR:   Q17WM2
- STRING:   Q17WM2
- GeneID:   4177756
- GenomeReviews:   AM260522_GR
- KEGG:   hac:Hac_1198
- NMPDR:   fig|382638.8.peg.1163
- eggNOG:   COG0154
- HOGENOM:   HBG481888
- OMA:   TESSCYG
- ProtClustDB:   PRK00012
- BioCyc:   HACI382638:HAC_1198-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 49559; Mature: 49559

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 53-53 ACT_SITE 128-128 ACT_SITE 152-152

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITLKQALSLSQDELETLKNGIDANVRASDLNAYIKAPSLNGASAKGVPILIKDNISVKG
CCCHHHHHCCCHHHHHHHHCCCCCCEEECCCCEEEECCCCCCCCCCCCEEEEECCCEEEE
WEITCSSKILEGYVAPYHASVIENLHQNSMAGFGLSNMDEFAMGSTTESSCYGITKNPRD
EEEEEHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCC
KNRVPGGSSGGSAAAVAGGLAVAALGSDTGGSIRQPASYCGCVGLKPTYGRVSRYGLIAY
CCCCCCCCCCCCHHHHHCCEEEEEECCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHH
CSSFDQIGPITQNVEDASILFDAISGHDSKDSTSANLKPTQTFKNLNRERRFKIAVLRDH
HHCHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCHHHHEEHHHHHHH
IKDASNEVQLAYENTLKALKEIGHEIVEKKILDSHYQISIYYIISMAEASSNLARFDGVR
HHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEHHHHCCHHHHCCCH
YGRRAQNIKDLKELYLKSRSEGFGDEVKRRIMLGNFVLSSGYYDAYYLKAQQMRLMIKEQ
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHH
YNKIFEEVDLIFTPVAPTTAPLFNYHASPLEMYLSDIYTIGANLSGLPALSLPVAKDPLG
HHHHHHHHHEEEECCCCCCCHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
LPIGMQFIAKAFDEQSLLDVSYALEQELKLKLD
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHEECC
>Mature Secondary Structure
MITLKQALSLSQDELETLKNGIDANVRASDLNAYIKAPSLNGASAKGVPILIKDNISVKG
CCCHHHHHCCCHHHHHHHHCCCCCCEEECCCCEEEECCCCCCCCCCCCEEEEECCCEEEE
WEITCSSKILEGYVAPYHASVIENLHQNSMAGFGLSNMDEFAMGSTTESSCYGITKNPRD
EEEEEHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCC
KNRVPGGSSGGSAAAVAGGLAVAALGSDTGGSIRQPASYCGCVGLKPTYGRVSRYGLIAY
CCCCCCCCCCCCHHHHHCCEEEEEECCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHH
CSSFDQIGPITQNVEDASILFDAISGHDSKDSTSANLKPTQTFKNLNRERRFKIAVLRDH
HHCHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCHHHHEEHHHHHHH
IKDASNEVQLAYENTLKALKEIGHEIVEKKILDSHYQISIYYIISMAEASSNLARFDGVR
HHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEHHHHCCHHHHCCCH
YGRRAQNIKDLKELYLKSRSEGFGDEVKRRIMLGNFVLSSGYYDAYYLKAQQMRLMIKEQ
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHH
YNKIFEEVDLIFTPVAPTTAPLFNYHASPLEMYLSDIYTIGANLSGLPALSLPVAKDPLG
HHHHHHHHHEEEECCCCCCCHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
LPIGMQFIAKAFDEQSLLDVSYALEQELKLKLD
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA