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The map label for this gene is metS [H]

Identifier: 109947187

GI number: 109947187

Start: 531922

End: 533853

Strand: Direct

Name: metS [H]

Synonym: Hac_0605

Alternate gene names: 109947187

Gene position: 531922-533853 (Clockwise)

Preceding gene: 109947185

Following gene: 109947188

Centisome position: 34.23

GC content: 36.85

Gene sequence:

>1932_bases
ATGCAAAAATCACTGATCACAACCCCCATTTATTATGTGAATGATATCCCCCATATTGGTCATGCTTATACGACTTTGAT
TGCAGACACTCTAAAGAAGTATTACACGCTTCAAGGCGAAGAAGTCTTTTTTTTAACTGGCACCGATGAGCATGGGCAAA
AGATTGAACAAAGTGCGAAATTAAGAAATCAAAGCCCTAAAGCTTACGCCGATAGCATTAGCGCGATTTTTAAAAACCAA
TGGGATTTTTTCAATTTAGATTATGATGGTTTTATCCGCACCACAGATAATGAACATCAAAAATGCGTGCAAAACGCTTT
TGAAATCATGTTTGAAAAAGGGGATATTTATAAAGGTACTTATAGTGGGTATTATTGCGTGAGTTGTGAGAGTTATTGCG
CGATCTCTAAAGCGGATAATATAAACAATAAAGTCTTATGCCCTGATTGTTTGAGAGAGACCACGCTTTTAGAAGAAGAG
AGTTATTTTTTTAGATTGAGTGCATATGAAAAACCTTTATTAGAGTTTTATGCCAAAAACCCTGAAGTGATCTTGCCCAT
TTATCGTAAAAATGAAGTCACTTCTTTTATTGAACAAGGCTTGTTGGATCTATCTATCACGCGCACAAGCTTTGAATGGG
GCATTCTTTTACCTAAAAAAATGAATGATCCTAAACATGTGGTGTATGTTTGGCTAGACGCTTTATTGAATTATGCGAGC
GCGTTGGGGTATTTGAATGGTTTAGAAAATAAAATGGTGCATTTTGAGTGCGCTAGGCATATTGTGGGTAAGGATATTTT
ACGCTTCCATGCCATTTATTGGCCAGCCTTTTTGATGAGCTTGGGTTTGCCCTTATTCAAACAGCTTTGCGTGCATGGGT
GGTGGACGATAGAGGGCGTGAAAATGAGTAAGAGCTTGGGTAATGTTTTAGACGCTCAAAAGCTTGCTATGGAGTATGGG
ATTGAAGAATTGCGCTATTTTTTATTGCGTGAAGTACCTTTTGGGCAAGATGGGGATTTTTCTAAAAAAGCGTTAGTAGA
AAGGATCAACGCCAATTTGAACAACGATTTGGGGAATTTATTGAATCGTTTGTTAGGCATGGCTAAAAAATATTTTAACT
ATTCTTTAAAAAGCGAAAAAATCACCACATATTACCCTAAAGAGTTAGAAAAAGTGCATCAAATTTTAGATAACGCTAAC
TCTTTTGTGCCTAAAATGCAATTGCATAAAGCGTTAGAAGAATTGTTTGGCGTTTATGATTTTTTAAACAAACTCATCGC
TAAAGAAGAGCCATGGGTTTTGCATAAAAACAACGAATCAGAAAAATTAGAAGCCTTATTGAGTTTGATTGCAAACGCGC
TTTTACAATCAAGCTTTTTACTCTATGCGTTCATGCCAAAGAGTGCTATGAAATTAGCGAGCGCTTTTAATGTAAAAATC
ACGCCCACAAATTACGAACGCTTTTTCAAGTCTAAAAATTTACAAGATATAATCTTGCAAGACACCGAGCCCTTATTCTC
TAAAATGGAAAAAGTGGGGGAAACAACCCCAGCCAAAAAAGAAGAAGAAAAAACGCCAGAAGAAAAAACCCCACAAAAAC
AAGAAAACTATATCAGTATTGATGATTTCAAAAAAGTAGAAATTAAAGTGGGGCTTATCAAAGAGGCTCAAAGGGTTGAA
AAGTCCAATAAATTACTGCGCTTAAAGGTGGATTTAGGTGAAAATCGTTTGAGGCAGATTATTTCAGGGATCGCTTTGGA
TTATGAGCCTGAAAATTTAATCGGTCAAATGGTGTGCGTGGTGGCTAATTTAAAATCCGCAAAGCTTATGGGCGAAATGA
GTGAGGGCATGATTTTAGCGGTGCGAGATAATGACAGTTTGGCCTTAATTAGCCCCACAAAAGAAAAAACCGCAGGAAGT
CTCATTAGCTAA

Upstream 100 bases:

>100_bases
AATTTAACCTCTAGCCTCTAAATTGCATGGCTTTATTTTCAAAAGCATGTTAAAATTAATGCGAATTTCTAAAACAAATT
AAGGTAACGCTATTACAAAA

Downstream 100 bases:

>100_bases
ATGCGATTAGGGGTGAATGAAGCGGTAGAGTTGAGTTTGGGCGAATTGCAAAACACGCCCTCAATCAGCTATTTTAATTC
TATTGTTTTGTCTTTAAACA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 643; Mature: 643

Protein sequence:

>643_residues
MQKSLITTPIYYVNDIPHIGHAYTTLIADTLKKYYTLQGEEVFFLTGTDEHGQKIEQSAKLRNQSPKAYADSISAIFKNQ
WDFFNLDYDGFIRTTDNEHQKCVQNAFEIMFEKGDIYKGTYSGYYCVSCESYCAISKADNINNKVLCPDCLRETTLLEEE
SYFFRLSAYEKPLLEFYAKNPEVILPIYRKNEVTSFIEQGLLDLSITRTSFEWGILLPKKMNDPKHVVYVWLDALLNYAS
ALGYLNGLENKMVHFECARHIVGKDILRFHAIYWPAFLMSLGLPLFKQLCVHGWWTIEGVKMSKSLGNVLDAQKLAMEYG
IEELRYFLLREVPFGQDGDFSKKALVERINANLNNDLGNLLNRLLGMAKKYFNYSLKSEKITTYYPKELEKVHQILDNAN
SFVPKMQLHKALEELFGVYDFLNKLIAKEEPWVLHKNNESEKLEALLSLIANALLQSSFLLYAFMPKSAMKLASAFNVKI
TPTNYERFFKSKNLQDIILQDTEPLFSKMEKVGETTPAKKEEEKTPEEKTPQKQENYISIDDFKKVEIKVGLIKEAQRVE
KSNKLLRLKVDLGENRLRQIISGIALDYEPENLIGQMVCVVANLKSAKLMGEMSEGMILAVRDNDSLALISPTKEKTAGS
LIS

Sequences:

>Translated_643_residues
MQKSLITTPIYYVNDIPHIGHAYTTLIADTLKKYYTLQGEEVFFLTGTDEHGQKIEQSAKLRNQSPKAYADSISAIFKNQ
WDFFNLDYDGFIRTTDNEHQKCVQNAFEIMFEKGDIYKGTYSGYYCVSCESYCAISKADNINNKVLCPDCLRETTLLEEE
SYFFRLSAYEKPLLEFYAKNPEVILPIYRKNEVTSFIEQGLLDLSITRTSFEWGILLPKKMNDPKHVVYVWLDALLNYAS
ALGYLNGLENKMVHFECARHIVGKDILRFHAIYWPAFLMSLGLPLFKQLCVHGWWTIEGVKMSKSLGNVLDAQKLAMEYG
IEELRYFLLREVPFGQDGDFSKKALVERINANLNNDLGNLLNRLLGMAKKYFNYSLKSEKITTYYPKELEKVHQILDNAN
SFVPKMQLHKALEELFGVYDFLNKLIAKEEPWVLHKNNESEKLEALLSLIANALLQSSFLLYAFMPKSAMKLASAFNVKI
TPTNYERFFKSKNLQDIILQDTEPLFSKMEKVGETTPAKKEEEKTPEEKTPQKQENYISIDDFKKVEIKVGLIKEAQRVE
KSNKLLRLKVDLGENRLRQIISGIALDYEPENLIGQMVCVVANLKSAKLMGEMSEGMILAVRDNDSLALISPTKEKTAGS
LIS
>Mature_643_residues
MQKSLITTPIYYVNDIPHIGHAYTTLIADTLKKYYTLQGEEVFFLTGTDEHGQKIEQSAKLRNQSPKAYADSISAIFKNQ
WDFFNLDYDGFIRTTDNEHQKCVQNAFEIMFEKGDIYKGTYSGYYCVSCESYCAISKADNINNKVLCPDCLRETTLLEEE
SYFFRLSAYEKPLLEFYAKNPEVILPIYRKNEVTSFIEQGLLDLSITRTSFEWGILLPKKMNDPKHVVYVWLDALLNYAS
ALGYLNGLENKMVHFECARHIVGKDILRFHAIYWPAFLMSLGLPLFKQLCVHGWWTIEGVKMSKSLGNVLDAQKLAMEYG
IEELRYFLLREVPFGQDGDFSKKALVERINANLNNDLGNLLNRLLGMAKKYFNYSLKSEKITTYYPKELEKVHQILDNAN
SFVPKMQLHKALEELFGVYDFLNKLIAKEEPWVLHKNNESEKLEALLSLIANALLQSSFLLYAFMPKSAMKLASAFNVKI
TPTNYERFFKSKNLQDIILQDTEPLFSKMEKVGETTPAKKEEEKTPEEKTPQKQENYISIDDFKKVEIKVGLIKEAQRVE
KSNKLLRLKVDLGENRLRQIISGIALDYEPENLIGQMVCVVANLKSAKLMGEMSEGMILAVRDNDSLALISPTKEKTAGS
LIS

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=510, Percent_Identity=34.5098039215686, Blast_Score=281, Evalue=2e-75,
Organism=Homo sapiens, GI14043022, Length=554, Percent_Identity=25.2707581227437, Blast_Score=139, Evalue=7e-33,
Organism=Escherichia coli, GI1788432, Length=691, Percent_Identity=26.7727930535456, Blast_Score=219, Evalue=5e-58,
Organism=Escherichia coli, GI1789455, Length=104, Percent_Identity=35.5769230769231, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI212645096, Length=485, Percent_Identity=35.0515463917526, Blast_Score=258, Evalue=1e-68,
Organism=Caenorhabditis elegans, GI71992781, Length=375, Percent_Identity=34.1333333333333, Blast_Score=177, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17540794, Length=582, Percent_Identity=25.2577319587629, Blast_Score=156, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6321610, Length=480, Percent_Identity=34.5833333333333, Blast_Score=264, Evalue=4e-71,
Organism=Saccharomyces cerevisiae, GI6321704, Length=521, Percent_Identity=24.1842610364683, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI21358483, Length=556, Percent_Identity=32.1942446043165, Blast_Score=269, Evalue=4e-72,
Organism=Drosophila melanogaster, GI24655311, Length=561, Percent_Identity=25.8467023172906, Blast_Score=163, Evalue=4e-40,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 73791; Mature: 73791

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKSLITTPIYYVNDIPHIGHAYTTLIADTLKKYYTLQGEEVFFLTGTDEHGQKIEQSAK
CCCCEECCCCCEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHH
LRNQSPKAYADSISAIFKNQWDFFNLDYDGFIRTTDNEHQKCVQNAFEIMFEKGDIYKGT
HHCCCCHHHHHHHHHHHHCCCCEEECCCCCEEECCCCHHHHHHHHHHHHHHHCCCEEECC
YSGYYCVSCESYCAISKADNINNKVLCPDCLRETTLLEEESYFFRLSAYEKPLLEFYAKN
CCCEEEECCCCCEEECCCCCCCCCEECHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHCCC
PEVILPIYRKNEVTSFIEQGLLDLSITRTSFEWGILLPKKMNDPKHVVYVWLDALLNYAS
CCEEEEEECCHHHHHHHHCCCCEEEEEEECCEEEEEECCCCCCCHHHHHHHHHHHHHHHH
ALGYLNGLENKMVHFECARHIVGKDILRFHAIYWPAFLMSLGLPLFKQLCVHGWWTIEGV
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCEEEECE
KMSKSLGNVLDAQKLAMEYGIEELRYFLLREVPFGQDGDFSKKALVERINANLNNDLGNL
EHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCHHHHH
LNRLLGMAKKYFNYSLKSEKITTYYPKELEKVHQILDNANSFVPKMQLHKALEELFGVYD
HHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHCHHHCCHHHHHHHHHHHHHHHHH
FLNKLIAKEEPWVLHKNNESEKLEALLSLIANALLQSSFLLYAFMPKSAMKLASAFNVKI
HHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHCEEEE
TPTNYERFFKSKNLQDIILQDTEPLFSKMEKVGETTPAKKEEEKTPEEKTPQKQENYISI
CCCHHHHHHHCCCCHHHHHCCCHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCHHCCEECC
DDFKKVEIKVGLIKEAQRVEKSNKLLRLKVDLGENRLRQIISGIALDYEPENLIGQMVCV
CCCEEEEEEEEHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHH
VANLKSAKLMGEMSEGMILAVRDNDSLALISPTKEKTAGSLIS
HHCCHHHHHHHHCCCCEEEEEECCCCEEEECCCCHHCCCCCCC
>Mature Secondary Structure
MQKSLITTPIYYVNDIPHIGHAYTTLIADTLKKYYTLQGEEVFFLTGTDEHGQKIEQSAK
CCCCEECCCCCEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHH
LRNQSPKAYADSISAIFKNQWDFFNLDYDGFIRTTDNEHQKCVQNAFEIMFEKGDIYKGT
HHCCCCHHHHHHHHHHHHCCCCEEECCCCCEEECCCCHHHHHHHHHHHHHHHCCCEEECC
YSGYYCVSCESYCAISKADNINNKVLCPDCLRETTLLEEESYFFRLSAYEKPLLEFYAKN
CCCEEEECCCCCEEECCCCCCCCCEECHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHCCC
PEVILPIYRKNEVTSFIEQGLLDLSITRTSFEWGILLPKKMNDPKHVVYVWLDALLNYAS
CCEEEEEECCHHHHHHHHCCCCEEEEEEECCEEEEEECCCCCCCHHHHHHHHHHHHHHHH
ALGYLNGLENKMVHFECARHIVGKDILRFHAIYWPAFLMSLGLPLFKQLCVHGWWTIEGV
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCEEEECE
KMSKSLGNVLDAQKLAMEYGIEELRYFLLREVPFGQDGDFSKKALVERINANLNNDLGNL
EHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCHHHHH
LNRLLGMAKKYFNYSLKSEKITTYYPKELEKVHQILDNANSFVPKMQLHKALEELFGVYD
HHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHCHHHCCHHHHHHHHHHHHHHHHH
FLNKLIAKEEPWVLHKNNESEKLEALLSLIANALLQSSFLLYAFMPKSAMKLASAFNVKI
HHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHCEEEE
TPTNYERFFKSKNLQDIILQDTEPLFSKMEKVGETTPAKKEEEKTPEEKTPQKQENYISI
CCCHHHHHHHCCCCHHHHHCCCHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCHHCCEECC
DDFKKVEIKVGLIKEAQRVEKSNKLLRLKVDLGENRLRQIISGIALDYEPENLIGQMVCV
CCCEEEEEEEEHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHH
VANLKSAKLMGEMSEGMILAVRDNDSLALISPTKEKTAGSLIS
HHCCHHHHHHHHCCCCEEEEEECCCCEEEECCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]