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The map label for this gene is dsbE [H]

Identifier: 108812368

GI number: 108812368

Start: 2493535

End: 2494254

Strand: Reverse

Name: dsbE [H]

Synonym: YPN_2207

Alternate gene names: 108812368

Gene position: 2494254-2493535 (Counterclockwise)

Preceding gene: 108812369

Following gene: 108812367

Centisome position: 55.01

GC content: 49.58

Gene sequence:

>720_bases
ATGAAACCTCAATCCTCATTCAATAAGCTGATGTTTATTCCGCTGGTCCTTTTCTTACTGCTGGTCGTGGCATTCCTGAT
ACAACTGACCCGTAATGCCAACGGCGAAGATCCAACGATGTTGGAGTCGGCGCTGATCGGCAAGCCCGTACCGGCATTCA
AACTGGAATCCCTCGAACAGCCGGGTAAAACCTTTGATCAGGCGGTATTACGTGATGGTAAACCGATACTGCTGAATGTT
TGGGCAACATGGTGCCCAACTTGCCGTGCCGAGCATGAATACCTGAATAAATTGGCGGCTCAAGGTATCCGTGTGGTGGG
CCTGAATTATAAAGATGACCGTAGCAAAGCCGTGCAGTGGCTGAATTCGCTGGGTAACCCTTATTTTCTCAGTTTGTATG
ATGGTGATGGAATGTTGGGCCTGGATTTGGGGGTATACGGCGCACCAGAAACGTTCCTTATCGATGGTCAAGGGGTTATC
CGTTACCGCCATGCTGGTGATCTTAATGATCGGGTCTGGCAGCAGGAAATCTTACCGCTGTATAAAAAGTACCAGGGGGC
GCATGAGGTTACTCAGTCTGCTACTGGGTCTGATATTGAGCTGGGGAGTATTGGCGACCATCGATACTTATCGCTTCAGT
TCGGTAGAACAAGAGCAGCAATACCGTGCACTGACCGAGCAACTGCGTTGCCCAAAATGCCAGAACAACAGTATTGCTGA

Upstream 100 bases:

>100_bases
GCGGGATCTTTATGGCCGTCGGCGGCATCTTCTGTATGCTCGATCCGCGCTATCGGATGAGTAAAAAGCTAAAACGCGGT
GCGATACCGGAGGCAGAGTC

Downstream 100 bases:

>100_bases
CTCGAATGCCACCATTGCGGCCGATATGCGCGCTAAAGTGTATGAACTGATGCAACAAGGTTATTCCAAACAGCAGATTG
TTGATTATATGGTGGCCCGC

Product: thiol:disulfide interchange protein DsbE

Products: NA

Alternate protein names: Cytochrome c biogenesis protein CcmG [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MKPQSSFNKLMFIPLVLFLLLVVAFLIQLTRNANGEDPTMLESALIGKPVPAFKLESLEQPGKTFDQAVLRDGKPILLNV
WATWCPTCRAEHEYLNKLAAQGIRVVGLNYKDDRSKAVQWLNSLGNPYFLSLYDGDGMLGLDLGVYGAPETFLIDGQGVI
RYRHAGDLNDRVWQQEILPLYKKYQGAHEVTQSATGSDIELGSIGDHRYLSLQFGRTRAAIPCTDRATALPKMPEQQYC

Sequences:

>Translated_239_residues
MKPQSSFNKLMFIPLVLFLLLVVAFLIQLTRNANGEDPTMLESALIGKPVPAFKLESLEQPGKTFDQAVLRDGKPILLNV
WATWCPTCRAEHEYLNKLAAQGIRVVGLNYKDDRSKAVQWLNSLGNPYFLSLYDGDGMLGLDLGVYGAPETFLIDGQGVI
RYRHAGDLNDRVWQQEILPLYKKYQGAHEVTQSATGSDIELGSIGDHRYLSLQFGRTRAAIPCTDRATALPKMPEQQYC
>Mature_239_residues
MKPQSSFNKLMFIPLVLFLLLVVAFLIQLTRNANGEDPTMLESALIGKPVPAFKLESLEQPGKTFDQAVLRDGKPILLNV
WATWCPTCRAEHEYLNKLAAQGIRVVGLNYKDDRSKAVQWLNSLGNPYFLSLYDGDGMLGLDLGVYGAPETFLIDGQGVI
RYRHAGDLNDRVWQQEILPLYKKYQGAHEVTQSATGSDIELGSIGDHRYLSLQFGRTRAAIPCTDRATALPKMPEQQYC

Specific function: Involved in disulfide bond formation. Catalyzes a late, reductive step in the assembly of periplasmic c-type cytochromes, probably the reduction of disulfide bonds of the apocytochrome c to allow covalent linkage with the heme. Possible subunit of a heme

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein; Periplasmic side [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Escherichia coli, GI1788523, Length=176, Percent_Identity=73.2954545454545, Blast_Score=283, Evalue=6e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004799
- InterPro:   IPR013740
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335 [H]

Pfam domain/function: PF08534 Redoxin [H]

EC number: NA

Molecular weight: Translated: 26719; Mature: 26719

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS00194 THIOREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPQSSFNKLMFIPLVLFLLLVVAFLIQLTRNANGEDPTMLESALIGKPVPAFKLESLEQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHCC
PGKTFDQAVLRDGKPILLNVWATWCPTCRAEHEYLNKLAAQGIRVVGLNYKDDRSKAVQW
CCHHHHHHHHHCCCCEEEEEHHHHCCCCCHHHHHHHHHHHCCEEEEECCCCCCHHHHHHH
LNSLGNPYFLSLYDGDGMLGLDLGVYGAPETFLIDGQGVIRYRHAGDLNDRVWQQEILPL
HHHCCCCEEEEEECCCCEEEEEECCCCCCCEEEECCCCEEEEECCCCCHHHHHHHHHHHH
YKKYQGAHEVTQSATGSDIELGSIGDHRYLSLQFGRTRAAIPCTDRATALPKMPEQQYC
HHHHCCHHHHHHCCCCCCEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKPQSSFNKLMFIPLVLFLLLVVAFLIQLTRNANGEDPTMLESALIGKPVPAFKLESLEQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHCC
PGKTFDQAVLRDGKPILLNVWATWCPTCRAEHEYLNKLAAQGIRVVGLNYKDDRSKAVQW
CCHHHHHHHHHCCCCEEEEEHHHHCCCCCHHHHHHHHHHHCCEEEEECCCCCCHHHHHHH
LNSLGNPYFLSLYDGDGMLGLDLGVYGAPETFLIDGQGVIRYRHAGDLNDRVWQQEILPL
HHHCCCCEEEEEECCCCEEEEEECCCCCCCEEEECCCCEEEEECCCCCHHHHHHHHHHHH
YKKYQGAHEVTQSATGSDIELGSIGDHRYLSLQFGRTRAAIPCTDRATALPKMPEQQYC
HHHHCCHHHHHHCCCCCCEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]