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The map label for this gene is nuoM [H]

Identifier: 108812301

GI number: 108812301

Start: 2418361

End: 2419893

Strand: Reverse

Name: nuoM [H]

Synonym: YPN_2139

Alternate gene names: 108812301

Gene position: 2419893-2418361 (Counterclockwise)

Preceding gene: 108812302

Following gene: 108812300

Centisome position: 53.37

GC content: 51.4

Gene sequence:

>1533_bases
ATGCTATTACCTTGGCTAATTCTTATCCCCTTTATCGGCGGTCTGCTGTGCTGGCAGCTTGAACGCTTCGGTACCAAAGT
ACCGCGTTGGATTGCGTTAATCGCGATGGGGCTGACATTGGTGCTTTCCCTGTATCTGTGGGGGCAGGGCAACTATTCAC
TGATGAACCCGACGGGTATTCCGCAGTGGCAGACATATTATAATGTGCCGTGGATCCCACGTTTCGGCATTAGATTCCAT
TTAGCGTTGGACGGTTTGTCCTTGCTTATGGTGGTGCTAACCGGCCTACTTGGTGTACTGGCGATCCTCTGTTCATGGCG
TGAAATTCAGCGCTATCAGGGTTTCTTCCACCTGAATCTGTTATGGATTCTGGGGGGGGTGATCGGCGTGTTCTTGGCTA
TCGACATGTTCCTGTTCTTCTTCTTCTGGGAAATGATGTTGGTACCCATGTACTTCCTAATTGCCTTGTGGGGCCATAAA
GCCTCAGACGGTAAAACCCGGATCACCGCGGCGACCAAGTTCTTTATTTATACGCAGGCCAGCGGCTTGATTATGTTGAT
TGCCATTCTGGGGCTGGTATTTGTGCACTTCAATGCCACCGGTATTTGGACATTCAACTACGAAGATCTGTTGAACACTC
CGATGTCGCGGAATGTAGAATATCTGCTGATGCTGGGTTTCTTTATCGCCTTTGCGGTAAAAATGCCAGTGGTGCCGTTA
CATGGCTGGTTGCCGGATGCACACAGCCAGGCACCTACCGCCGGGTCTGTTGACCTTGCGGGGATTTTGCTGAAGACCGC
CGCTTATGGTTTGCTGCGTTTCAGCCTGCCTCTGTTCCCGAATGCGTCTCATGAGTTTGCCCCTATCGCTATGTGGTTAG
GGGTTATCGGCATCTTCTACGGTGCATGGATGGCGTTTTGCCAAACAGATATCAAACGTCTTATTGCTTACACCAGCGTG
TCGCACATGGGCTTCGTATTGATTGCCATCTATACCGGCAGCCAACTGGCTTATCAGGGCGCGGTAATTCAGATGATTGC
TCACGGTTTATCAGCCGCGGGGATGTTCATTATCTGTGGTCAGTTGTACGAACGTTTGCATACGCGTGATATGCGCCAGA
TGGGCGGTTTGTGGGGGCGGATAAAATACCTGCCTGCGCTCTCCCTGTTCTTCGCTGTCGCGACATTGGGGATGCCGGGG
ACCGGTAACTTCGTCGGCGAATTCATGATCCTATTCGGCAGTTTCCAGGTCGTTCCAGTGATTACGGTGATCGCGACCTT
TGGCTTGGTATTTGCGTCGGTTTACTCGCTTATTATGATGCAGCGTGCTTATTACGGTACGCCAAAATCGGAGGAAGCCT
TACCGGGTATGACCGCGCGAGAACTGTCCATCGTCTTGCTGCTGGTGGTGTTGCTTGTGTTGCTGGGGGTTTACCCGCAG
CCGATCCTCGACACGTCCCATGCGGCAATGAGTAATGTGCAACAGTGGTTTTCAGCTGCATCTATTCCAGCTCCATCTAT
TACAAGGCCATAA

Upstream 100 bases:

>100_bases
GTACGCCGCCTCTATAGGTTTGGGTGCAGTGATTGTGCTGGCTCTGTTGCTTTTCGTGTAGATGCCTGAATAGGCTCATT
AATTAGGGACACAAAACGCC

Downstream 100 bases:

>100_bases
TTCGCCATGACAATAACTCCTCAACAACTGATCGCAATGCTACCGCTGTTGATCGTCGGATTGACGGTGGTGGTTGTGAT
GCTGTCCATTGCGTGGCGAC

Product: NADH dehydrogenase subunit M

Products: NA

Alternate protein names: NADH dehydrogenase I subunit M; NDH-1 subunit M; NUO13 [H]

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MLLPWLILIPFIGGLLCWQLERFGTKVPRWIALIAMGLTLVLSLYLWGQGNYSLMNPTGIPQWQTYYNVPWIPRFGIRFH
LALDGLSLLMVVLTGLLGVLAILCSWREIQRYQGFFHLNLLWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
ASDGKTRITAATKFFIYTQASGLIMLIAILGLVFVHFNATGIWTFNYEDLLNTPMSRNVEYLLMLGFFIAFAVKMPVVPL
HGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSLPLFPNASHEFAPIAMWLGVIGIFYGAWMAFCQTDIKRLIAYTSV
SHMGFVLIAIYTGSQLAYQGAVIQMIAHGLSAAGMFIICGQLYERLHTRDMRQMGGLWGRIKYLPALSLFFAVATLGMPG
TGNFVGEFMILFGSFQVVPVITVIATFGLVFASVYSLIMMQRAYYGTPKSEEALPGMTARELSIVLLLVVLLVLLGVYPQ
PILDTSHAAMSNVQQWFSAASIPAPSITRP

Sequences:

>Translated_510_residues
MLLPWLILIPFIGGLLCWQLERFGTKVPRWIALIAMGLTLVLSLYLWGQGNYSLMNPTGIPQWQTYYNVPWIPRFGIRFH
LALDGLSLLMVVLTGLLGVLAILCSWREIQRYQGFFHLNLLWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
ASDGKTRITAATKFFIYTQASGLIMLIAILGLVFVHFNATGIWTFNYEDLLNTPMSRNVEYLLMLGFFIAFAVKMPVVPL
HGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSLPLFPNASHEFAPIAMWLGVIGIFYGAWMAFCQTDIKRLIAYTSV
SHMGFVLIAIYTGSQLAYQGAVIQMIAHGLSAAGMFIICGQLYERLHTRDMRQMGGLWGRIKYLPALSLFFAVATLGMPG
TGNFVGEFMILFGSFQVVPVITVIATFGLVFASVYSLIMMQRAYYGTPKSEEALPGMTARELSIVLLLVVLLVLLGVYPQ
PILDTSHAAMSNVQQWFSAASIPAPSITRP
>Mature_510_residues
MLLPWLILIPFIGGLLCWQLERFGTKVPRWIALIAMGLTLVLSLYLWGQGNYSLMNPTGIPQWQTYYNVPWIPRFGIRFH
LALDGLSLLMVVLTGLLGVLAILCSWREIQRYQGFFHLNLLWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
ASDGKTRITAATKFFIYTQASGLIMLIAILGLVFVHFNATGIWTFNYEDLLNTPMSRNVEYLLMLGFFIAFAVKMPVVPL
HGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSLPLFPNASHEFAPIAMWLGVIGIFYGAWMAFCQTDIKRLIAYTSV
SHMGFVLIAIYTGSQLAYQGAVIQMIAHGLSAAGMFIICGQLYERLHTRDMRQMGGLWGRIKYLPALSLFFAVATLGMPG
TGNFVGEFMILFGSFQVVPVITVIATFGLVFASVYSLIMMQRAYYGTPKSEEALPGMTARELSIVLLLVVLLVLLGVYPQ
PILDTSHAAMSNVQQWFSAASIPAPSITRP

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=364, Percent_Identity=33.2417582417582, Blast_Score=165, Evalue=7e-41,
Organism=Homo sapiens, GI251831117, Length=348, Percent_Identity=27.8735632183908, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1788613, Length=513, Percent_Identity=85.3801169590643, Blast_Score=868, Evalue=0.0,
Organism=Escherichia coli, GI1788827, Length=470, Percent_Identity=24.468085106383, Blast_Score=115, Evalue=8e-27,
Organism=Escherichia coli, GI1788831, Length=421, Percent_Identity=26.603325415677, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1788614, Length=530, Percent_Identity=25.8490566037736, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI145693160, Length=438, Percent_Identity=27.3972602739726, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788829, Length=420, Percent_Identity=26.4285714285714, Blast_Score=73, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 56827; Mature: 56827

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLPWLILIPFIGGLLCWQLERFGTKVPRWIALIAMGLTLVLSLYLWGQGNYSLMNPTGI
CCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCC
PQWQTYYNVPWIPRFGIRFHLALDGLSLLMVVLTGLLGVLAILCSWREIQRYQGFFHLNL
CCCCCEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
LWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHKASDGKTRITAATKFFIYTQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEECC
SGLIMLIAILGLVFVHFNATGIWTFNYEDLLNTPMSRNVEYLLMLGFFIAFAVKMPVVPL
CHHHHHHHHHHHHHHHCCCEEEEEECHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEC
HGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSLPLFPNASHEFAPIAMWLGVIGIFY
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
GAWMAFCQTDIKRLIAYTSVSHMGFVLIAIYTGSQLAYQGAVIQMIAHGLSAAGMFIICG
HHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
QLYERLHTRDMRQMGGLWGRIKYLPALSLFFAVATLGMPGTGNFVGEFMILFGSFQVVPV
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCHHHHHH
ITVIATFGLVFASVYSLIMMQRAYYGTPKSEEALPGMTARELSIVLLLVVLLVLLGVYPQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
PILDTSHAAMSNVQQWFSAASIPAPSITRP
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLLPWLILIPFIGGLLCWQLERFGTKVPRWIALIAMGLTLVLSLYLWGQGNYSLMNPTGI
CCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCC
PQWQTYYNVPWIPRFGIRFHLALDGLSLLMVVLTGLLGVLAILCSWREIQRYQGFFHLNL
CCCCCEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
LWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHKASDGKTRITAATKFFIYTQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEECC
SGLIMLIAILGLVFVHFNATGIWTFNYEDLLNTPMSRNVEYLLMLGFFIAFAVKMPVVPL
CHHHHHHHHHHHHHHHCCCEEEEEECHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEC
HGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSLPLFPNASHEFAPIAMWLGVIGIFY
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
GAWMAFCQTDIKRLIAYTSVSHMGFVLIAIYTGSQLAYQGAVIQMIAHGLSAAGMFIICG
HHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
QLYERLHTRDMRQMGGLWGRIKYLPALSLFFAVATLGMPGTGNFVGEFMILFGSFQVVPV
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCHHHHHH
ITVIATFGLVFASVYSLIMMQRAYYGTPKSEEALPGMTARELSIVLLLVVLLVLLGVYPQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
PILDTSHAAMSNVQQWFSAASIPAPSITRP
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]